In order to study the pathogenesis of hypertension associated with noninsulin dependent diabetes mellitus (NIDDM), Plasma glucose, insulin levels at fasting and following an oral glucose load were measured. Na +K +pum...In order to study the pathogenesis of hypertension associated with noninsulin dependent diabetes mellitus (NIDDM), Plasma glucose, insulin levels at fasting and following an oral glucose load were measured. Na +K +pump and Ca 2+ pump activities of red blood cell membrane were also assessed. Hypertensive patients with normal or impaired glucose tolerance (NGT, or IGT) had hyperinsulinemia. Obese hypertensive patients also had hyperinsulinemia, while nonobese hypertensive patients had no hyperinsulinemia, but exhibited a delay in insulin response to oral glucose tolerance test (OGTT). In multivariate analysis, considering the factors of age, BMI and plasma glucose level, DBP were still positively related to both 30 min insulin level and IAUC, but negatively correlated to activities of Na +K +pump and Ca 2+ pump. These results demonstrated that a link between obesity, hpertension and NIDDM is the insulin resistance and/or hyperinsulinemia.展开更多
Objective To construct the human obese(ob) cDNA clone in the Chinese, and analyze the expression of the ob gene in adipose tissue of obese, non obese subjects and nooinsulin dependent diabetes mellitus (NIDDM) Chin...Objective To construct the human obese(ob) cDNA clone in the Chinese, and analyze the expression of the ob gene in adipose tissue of obese, non obese subjects and nooinsulin dependent diabetes mellitus (NIDDM) Chinese patients Methods A ob cDNA clone was isolated by reverse transcription polymerase chain reaction (RT PCR) Four groups of Chinese subjects participated in the study: 1) 12 obese subjects [body mass index (BMI): 28 5±2 3? kg/m 2]; 2) 11 non obese subjects (BMI: 21 0±1 5? kg/m 2); 3) 8 obese NIDDM patients (BMI: 27 0±1 4?kg/m 2); 4) 11 non obese NIDDM patients (BMI: 21 2±1 4?kg/m 2) The expression of ob gene mRNA in abdominal subcutaneous adipose tissue was examined using RNA dot blot hybridization with a digoxigenin labeled human ob cDNA probe The hybridized signals were quantitated by densitometry Results A full human ob cDNA fragment which included a glutamine codon at +49 was obtained A base substitution (A to G) in the coding region at position 287 was found, resulting in a glutamine being replaced by an arginine Expression of the ob gene was significantly higher in Chinese obese subjects compared to non obese ones ( P <0 05), and positively correlated with the BMI No significant difference in the amount of ob mRNA was detected between non diabetic and diabetic groups at the same BMI level Conclusions We constructed a full length human ob cDNA clone. The expression of the ob gene was significantly higher in Chinese obese subjects than in non obese ones. The metabolic and hormonal changes associated with NIDDM are not the main factors regulating the expression of the ob gene.展开更多
文摘In order to study the pathogenesis of hypertension associated with noninsulin dependent diabetes mellitus (NIDDM), Plasma glucose, insulin levels at fasting and following an oral glucose load were measured. Na +K +pump and Ca 2+ pump activities of red blood cell membrane were also assessed. Hypertensive patients with normal or impaired glucose tolerance (NGT, or IGT) had hyperinsulinemia. Obese hypertensive patients also had hyperinsulinemia, while nonobese hypertensive patients had no hyperinsulinemia, but exhibited a delay in insulin response to oral glucose tolerance test (OGTT). In multivariate analysis, considering the factors of age, BMI and plasma glucose level, DBP were still positively related to both 30 min insulin level and IAUC, but negatively correlated to activities of Na +K +pump and Ca 2+ pump. These results demonstrated that a link between obesity, hpertension and NIDDM is the insulin resistance and/or hyperinsulinemia.
文摘Objective To construct the human obese(ob) cDNA clone in the Chinese, and analyze the expression of the ob gene in adipose tissue of obese, non obese subjects and nooinsulin dependent diabetes mellitus (NIDDM) Chinese patients Methods A ob cDNA clone was isolated by reverse transcription polymerase chain reaction (RT PCR) Four groups of Chinese subjects participated in the study: 1) 12 obese subjects [body mass index (BMI): 28 5±2 3? kg/m 2]; 2) 11 non obese subjects (BMI: 21 0±1 5? kg/m 2); 3) 8 obese NIDDM patients (BMI: 27 0±1 4?kg/m 2); 4) 11 non obese NIDDM patients (BMI: 21 2±1 4?kg/m 2) The expression of ob gene mRNA in abdominal subcutaneous adipose tissue was examined using RNA dot blot hybridization with a digoxigenin labeled human ob cDNA probe The hybridized signals were quantitated by densitometry Results A full human ob cDNA fragment which included a glutamine codon at +49 was obtained A base substitution (A to G) in the coding region at position 287 was found, resulting in a glutamine being replaced by an arginine Expression of the ob gene was significantly higher in Chinese obese subjects compared to non obese ones ( P <0 05), and positively correlated with the BMI No significant difference in the amount of ob mRNA was detected between non diabetic and diabetic groups at the same BMI level Conclusions We constructed a full length human ob cDNA clone. The expression of the ob gene was significantly higher in Chinese obese subjects than in non obese ones. The metabolic and hormonal changes associated with NIDDM are not the main factors regulating the expression of the ob gene.
