Object: To localize the mRNA coding for VEGF at Ultrastractural level in human breast cancer by using digoxigenin-labeled cDNA probes. Methods: Nonradioactive in situ hybridization at electron microscopic level was em...Object: To localize the mRNA coding for VEGF at Ultrastractural level in human breast cancer by using digoxigenin-labeled cDNA probes. Methods: Nonradioactive in situ hybridization at electron microscopic level was employed to detected VEGF mRNA in breast cancer. Result: Cancer cells and endothelial cell of angiogensis show dark color in experiment sections. No dark color can be found in control sections. Positive hybridization signals showed dark dot and were located in various compartments of the breast cancer cell and endothelial cell in experiment section. No labeling was observed in control sections. In experiment sections, the staining appeared concentrated in cytoplasm and nucleus of the breast cancer cell and endothelial cell. Conclusion: Nonradioactive in situ hybridization at electron microscopic level is efficient for direct observation of the target site mRNA of VEGF in the cytoplasm and nucleus.展开更多
The nonradioactive in situ hybridization with anti digoxigenin goldand silver enhancement method was used to locate the exogenous gene on cheomosomes of transgenic pigs. After development, the gold silver particle...The nonradioactive in situ hybridization with anti digoxigenin goldand silver enhancement method was used to locate the exogenous gene on cheomosomes of transgenic pigs. After development, the gold silver particles could be easily detected via a light microscope. The experimental results demonstrated that the exogenous gene in the transgenic pig was integrated on chromosomes randomly.展开更多
基金This work was supported by NationalNatural Science Foundation of China (No. 39870314).
文摘Object: To localize the mRNA coding for VEGF at Ultrastractural level in human breast cancer by using digoxigenin-labeled cDNA probes. Methods: Nonradioactive in situ hybridization at electron microscopic level was employed to detected VEGF mRNA in breast cancer. Result: Cancer cells and endothelial cell of angiogensis show dark color in experiment sections. No dark color can be found in control sections. Positive hybridization signals showed dark dot and were located in various compartments of the breast cancer cell and endothelial cell in experiment section. No labeling was observed in control sections. In experiment sections, the staining appeared concentrated in cytoplasm and nucleus of the breast cancer cell and endothelial cell. Conclusion: Nonradioactive in situ hybridization at electron microscopic level is efficient for direct observation of the target site mRNA of VEGF in the cytoplasm and nucleus.
文摘The nonradioactive in situ hybridization with anti digoxigenin goldand silver enhancement method was used to locate the exogenous gene on cheomosomes of transgenic pigs. After development, the gold silver particles could be easily detected via a light microscope. The experimental results demonstrated that the exogenous gene in the transgenic pig was integrated on chromosomes randomly.
