The forming mechanism of microemulsion of sodium dodecyl sulfonate, alcohols, water and isooctane was studied, with particular emphasis on the effect of molecular weight and concentration of alcohols. Phase diagram of...The forming mechanism of microemulsion of sodium dodecyl sulfonate, alcohols, water and isooctane was studied, with particular emphasis on the effect of molecular weight and concentration of alcohols. Phase diagram of the four components, alcohol, sodium dodecyl sulfonate, water and isooctane, was used as a means of study, through which the microemulsion regions were deter-mined. Phase diagram of sodium dodecyl sulfonate/n-pentanol/isooctane/water system at km = 2 (km = Wn-pentanol / WSDS ) is presented. The variation of conductivities of different microemulsion samples with water was measured. From the conductivities we investigated a change in structure from water droplets in oil (W/O) at low water content to liquid crystal at intermediate water content and a struc-ture of oil droplets in water (O/W) at high water content.展开更多
The derivative fluorimetric characteristics of Tb—SDS and Tb—SDS—phenanthroline systems have been studied. The optimum conditions were obtained. The 2nd—derivative spectrofluorimetric method in the systems has bee...The derivative fluorimetric characteristics of Tb—SDS and Tb—SDS—phenanthroline systems have been studied. The optimum conditions were obtained. The 2nd—derivative spectrofluorimetric method in the systems has been applied to the determination of trace amounts of terbium, in rare earth ore and their oxides. The results are satisfactory.展开更多
Dodecyl benzenesulfonic acid(DBSA)surfactant was used in the present study to find the effect of concentrationon its electrical conductance in solution from 293-323 K above and below the critical micelle concentration...Dodecyl benzenesulfonic acid(DBSA)surfactant was used in the present study to find the effect of concentrationon its electrical conductance in solution from 293-323 K above and below the critical micelle concentration(CMC).Themicellization parameters i.e.degree of counter ion binding(β),aggregation number(n)and number of counter ion micelle(m)were measured.The interaction of DBSA with polyvinylpyrrolidone(PVP)was also studied at 293 K throughconductance and surface tension measure ments.A number of important parameters i.e.critical aggregation concentration(CAC),Gibb's free energy(△G)and binding ratio(R)were determined and the effect of NaCl on the CAC and polymersaturation point(PSP)was also investigated.展开更多
Background Keratinocytes play a crucial role in the biological function of skin barrier.The relationship between sodium lauryl sulfate （SLS） and keratinocytes has been studied.However,the cytotoxicity and effects of...Background Keratinocytes play a crucial role in the biological function of skin barrier.The relationship between sodium lauryl sulfate （SLS） and keratinocytes has been studied.However,the cytotoxicity and effects of sodium dodecyl benzene sulfonate （SDBS）,a common detergent similar to SLS,on keratinocytes are still not known.This study aimed to investigate the effects of SDBS on cytotoxicity and expression of proinflammatory cytokines in cultured human keratinocytes.Methods This study was carried out using the keratinocytes cell line,HaCaT cells.The cytotoxicity of SDBS on HaCaT cells was evaluated with cell counting kit-8 （CCK-8） and phase-contrast microscopy.After exposure to different concentrations of SDBS,the total RNA of the HaCaT cells was extracted for evaluating the relative mRNA expression of IL-1α,IL-6,IL-8,and TNF-α by qPCR.The supernatants of cells were collected for measuring the levels of IL-6 and IL-8 by enzyme-linked immunosorbent assay （ELISA）.Results SDBS at concentrations of 20 Jg/ml and over showed direct cytotoxicity and induced morphological changes of the HaCaT cells.The mRNA expressions of IL-1a,IL-6,IL-8,and TNF-α in different concentrations of SDBS at different time were comparable with that of controls.SDBS at concentrations of 5,10,and 15 μg/ml had no significant effects on IL-6 and IL-8 excretion from HaCaT cells after 24-hour exposure.Moreover,no significant effects on the IL-6 and IL-8 excretion were found after 10 and 15 μg/ml S DBS stimulations for 6,12,and 24 hours,respectively.Conclusion SDBS at higher concentrations had cytotoxicity on HaCaT cells but had no effects on the mRNA expression of IL-1α,IL-6,IL-8,and TNF-α,that was different from SLS.展开更多
Layered double hydroxides(LDHs)have been shown to be effective adsorbents for boron.However,solid-liquid separation is still a problem when separating boron from industrial radioactive waste liquid.In this research,th...Layered double hydroxides(LDHs)have been shown to be effective adsorbents for boron.However,solid-liquid separation is still a problem when separating boron from industrial radioactive waste liquid.In this research,three types of Mg-Al-LDHs including Mg-Al-LDH(NO_(3)^(-)),Mg-Al-LDH(Cl^(-))and Mg-Al-LDH(SO_(4)^(2-))were applied to adsorb boron,and moreover sodium dodecylbenzenesulfonate(SDBS)was used to float the LDH particles from aqueous solution after boron adsorption.The results showed that 60 min was sufficient for the equilibrium adsorption of the three LDHs.The boron adsorption capacity of three LDHs was determined as follows:Mg-Al-LDH(NO_(3)^(-))>Mg-Al-LDH(Cl^(-))>Mg-Al-LDH(SO_(4)^(2-)),and was 2.0,0.98 and 0.2 mmol·g^(-1),each ranging from 0 to 80 mmol·L^(-1)with the initial boron concentration.The efficiency of boron removal by Mg-Al-LDH(NO_(3)^(-))and SDBS can reach up to 89.7%.Furthermore,the boron flotation mechanism of SDBS and LDHs has been studied,since SDBS as a flotation agent can react with LDHs and penetrate into the interlayer of LDHs in addition to electrostatic attraction.Therefore,LDHs in solution can be floated onto the foam layer to be separated from the solution,and the clarified solution was obtained.The method is simple and promising for boron removal from aqueous solution.展开更多
The molecular non-covalent interaction often originates from the electrostatic attraction and accords with the Langmuir isothermal adsorption. The sodium dodecyl benzene sulfonate (SDBS)-polychrome blue B (PCB)-protei...The molecular non-covalent interaction often originates from the electrostatic attraction and accords with the Langmuir isothermal adsorption. The sodium dodecyl benzene sulfonate (SDBS)-polychrome blue B (PCB)-protein [bovine serum albumin (BSA), ovalbumin (OVA) and myoglobin (MB)] ternary reaction has been investigated at pH 3.88. Protein to replace PCB from the PCB-SDBS binding product was used to characterize the assembly of an invisible-spectral compound, SDBS, on proteins by measuring the variation of PCB light-absorption by the microsurface adsorption-spectral correction (MSASQ technique. The effect of ionic strength and temperature on the aggregation was studied. Results showed that the aggregates SDBS(92)(.)BSA, SDBS(58)(.)OVA and (SDBS15MB)-M-. at 30 degreesC and SDBS(83)(.)BSA, SDBS(39)(.)OVA and (SDBS10MB)-M-. at 50 degreesC are formed.展开更多
基金Supported by the Natural Science Foundation of Zhejiang Province (No. 299018).
文摘The forming mechanism of microemulsion of sodium dodecyl sulfonate, alcohols, water and isooctane was studied, with particular emphasis on the effect of molecular weight and concentration of alcohols. Phase diagram of the four components, alcohol, sodium dodecyl sulfonate, water and isooctane, was used as a means of study, through which the microemulsion regions were deter-mined. Phase diagram of sodium dodecyl sulfonate/n-pentanol/isooctane/water system at km = 2 (km = Wn-pentanol / WSDS ) is presented. The variation of conductivities of different microemulsion samples with water was measured. From the conductivities we investigated a change in structure from water droplets in oil (W/O) at low water content to liquid crystal at intermediate water content and a struc-ture of oil droplets in water (O/W) at high water content.
基金Project supported by the National Natural Science Foundation of China
文摘The derivative fluorimetric characteristics of Tb—SDS and Tb—SDS—phenanthroline systems have been studied. The optimum conditions were obtained. The 2nd—derivative spectrofluorimetric method in the systems has been applied to the determination of trace amounts of terbium, in rare earth ore and their oxides. The results are satisfactory.
文摘Dodecyl benzenesulfonic acid(DBSA)surfactant was used in the present study to find the effect of concentrationon its electrical conductance in solution from 293-323 K above and below the critical micelle concentration(CMC).Themicellization parameters i.e.degree of counter ion binding(β),aggregation number(n)and number of counter ion micelle(m)were measured.The interaction of DBSA with polyvinylpyrrolidone(PVP)was also studied at 293 K throughconductance and surface tension measure ments.A number of important parameters i.e.critical aggregation concentration(CAC),Gibb's free energy(△G)and binding ratio(R)were determined and the effect of NaCl on the CAC and polymersaturation point(PSP)was also investigated.
文摘Background Keratinocytes play a crucial role in the biological function of skin barrier.The relationship between sodium lauryl sulfate （SLS） and keratinocytes has been studied.However,the cytotoxicity and effects of sodium dodecyl benzene sulfonate （SDBS）,a common detergent similar to SLS,on keratinocytes are still not known.This study aimed to investigate the effects of SDBS on cytotoxicity and expression of proinflammatory cytokines in cultured human keratinocytes.Methods This study was carried out using the keratinocytes cell line,HaCaT cells.The cytotoxicity of SDBS on HaCaT cells was evaluated with cell counting kit-8 （CCK-8） and phase-contrast microscopy.After exposure to different concentrations of SDBS,the total RNA of the HaCaT cells was extracted for evaluating the relative mRNA expression of IL-1α,IL-6,IL-8,and TNF-α by qPCR.The supernatants of cells were collected for measuring the levels of IL-6 and IL-8 by enzyme-linked immunosorbent assay （ELISA）.Results SDBS at concentrations of 20 Jg/ml and over showed direct cytotoxicity and induced morphological changes of the HaCaT cells.The mRNA expressions of IL-1a,IL-6,IL-8,and TNF-α in different concentrations of SDBS at different time were comparable with that of controls.SDBS at concentrations of 5,10,and 15 μg/ml had no significant effects on IL-6 and IL-8 excretion from HaCaT cells after 24-hour exposure.Moreover,no significant effects on the IL-6 and IL-8 excretion were found after 10 and 15 μg/ml S DBS stimulations for 6,12,and 24 hours,respectively.Conclusion SDBS at higher concentrations had cytotoxicity on HaCaT cells but had no effects on the mRNA expression of IL-1α,IL-6,IL-8,and TNF-α,that was different from SLS.
基金financially supported by the National Natural Science Foundation of China(U20A20150)the National Key Research and Development Program of China(2018YFC1903802)+1 种基金the Youth Scientific Research Fund of Qinghai University(2022QGY-4)the Kunlun Talent Program of Qinghai Province。
文摘Layered double hydroxides(LDHs)have been shown to be effective adsorbents for boron.However,solid-liquid separation is still a problem when separating boron from industrial radioactive waste liquid.In this research,three types of Mg-Al-LDHs including Mg-Al-LDH(NO_(3)^(-)),Mg-Al-LDH(Cl^(-))and Mg-Al-LDH(SO_(4)^(2-))were applied to adsorb boron,and moreover sodium dodecylbenzenesulfonate(SDBS)was used to float the LDH particles from aqueous solution after boron adsorption.The results showed that 60 min was sufficient for the equilibrium adsorption of the three LDHs.The boron adsorption capacity of three LDHs was determined as follows:Mg-Al-LDH(NO_(3)^(-))>Mg-Al-LDH(Cl^(-))>Mg-Al-LDH(SO_(4)^(2-)),and was 2.0,0.98 and 0.2 mmol·g^(-1),each ranging from 0 to 80 mmol·L^(-1)with the initial boron concentration.The efficiency of boron removal by Mg-Al-LDH(NO_(3)^(-))and SDBS can reach up to 89.7%.Furthermore,the boron flotation mechanism of SDBS and LDHs has been studied,since SDBS as a flotation agent can react with LDHs and penetrate into the interlayer of LDHs in addition to electrostatic attraction.Therefore,LDHs in solution can be floated onto the foam layer to be separated from the solution,and the clarified solution was obtained.The method is simple and promising for boron removal from aqueous solution.
文摘The molecular non-covalent interaction often originates from the electrostatic attraction and accords with the Langmuir isothermal adsorption. The sodium dodecyl benzene sulfonate (SDBS)-polychrome blue B (PCB)-protein [bovine serum albumin (BSA), ovalbumin (OVA) and myoglobin (MB)] ternary reaction has been investigated at pH 3.88. Protein to replace PCB from the PCB-SDBS binding product was used to characterize the assembly of an invisible-spectral compound, SDBS, on proteins by measuring the variation of PCB light-absorption by the microsurface adsorption-spectral correction (MSASQ technique. The effect of ionic strength and temperature on the aggregation was studied. Results showed that the aggregates SDBS(92)(.)BSA, SDBS(58)(.)OVA and (SDBS15MB)-M-. at 30 degreesC and SDBS(83)(.)BSA, SDBS(39)(.)OVA and (SDBS10MB)-M-. at 50 degreesC are formed.
