The Influences of Mifepristone, Norethisterone and Tamoxifen on the Glycosphingolipids Compositions from Human Chorionic Tissue during Early Pregnancy

By using the Ladisch partitioning and microscale-analysis of HPTLC,the comparative quantitative and qualitative studies of gangliosides (Gg)and neutral glycosphingolipids(N-GSL)compositions from human chorionic villi tissues of normal early pregnant women and women treated with mifepristone, norethisterone(NET)and tamoxifen(TAM)were reported in this paper.The patterns of Gg and N-GSL in three treated groups were similar to those in normal pregnant group.The total values of Gg from the chorionic villi tissues reduced significantly in three treated groups(P<0.01).In all treated groups,the amounts of NeuNAC-Gal-Glc-cer(GM3),NeuNAC-NeuNAC-Gal-Glc-cer(GD3)and Gal-GalNAC-(NeuNAC)-Gal-Glc-cer(GM1)were decreased significantly compared with those in normal(P<0.01orP<0.05).In NET and TAM groups,Neu NAC-Gal-GalNAC-(NeuNAC-NeuNAC)-Gal-Glc-cer(GT1b) was markedly lower than that in normal(P<0.01).The total values of N-GSL extracted from the chorionic villi tissues were obviously higher in mifepristone and TAM groups than those in normal(P<0.01).The Gal-Glc-cer(LacCer)(CDH)and Gal-Gal-Glc-cer(Gal-LacCer)(CTH)were greatly increased in mifepristone group as compared with normal P<0.05).Paragloboside:Gal-GalNAC-Gal-Glc-cer(PG)in NETgroup was significantly higher than that in normal(P<0.01).

Studies on the Effects of Steroid Hormone Contraceptives on Lipid, Lipoprotein and Apolipoprotein Metabolism in Chinese Women

Objective To explore the effects of steroid hormone contraceptives (SHCs), such as Mercilon, Chinese No. 1 pill, Post coital pill, Mesigyna, and Cyclofem, on lipid metabolism in Chinese women during the last decade Materials & Methods A total of 122 healthy volunteers who requested contraception were recruited in the study and one of the above mentioned SHCs was administered.Fasting serum samples were taken from each subject before, during and after treatments. Triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL c), low density lipoprotein cholesterol (LDL c), Apolipoprotein A I, A II and B were measured. The changes in the ratios of TC/HDL c, Apo A I/Apo B were also observed. Results Although the levels of lipid were either increased or decreased by any one of the 5 contraceptives, these changes recovered after withdrawal of the drug administration. No significant changes were detected in the ratios of TC/HDL c, Apo A I/Apo B during treatments. Conclusion The results suggest that there is no obvious impact on lipid profile induced by any of the 5 contraceptives within one year. The induced effects on lipid metabolism could be reduced by alternate use of 2 contraceptives with different biological activities.