The increasing atmospheric carbon dioxide concentration, caused by fossil fuel combustion and deforestation, plays an important role in plant growth and development. Wheat, as a major staple crop, adapts to climate ch...The increasing atmospheric carbon dioxide concentration, caused by fossil fuel combustion and deforestation, plays an important role in plant growth and development. Wheat, as a major staple crop, adapts to climate change by tuning its inherent molecular mechanism, which is not well understood. The present study employed the RNA-Seq method to generate transcriptome profiles of the wheat Norin 10 in response to elevated CO_2 in comparison with ambient CO_2. The 10 895 787 high-quality clean reads of Norin 10 were assembled de novo using Trinity（without a reference genome） resulting in a total of 18 206 candidate transcripts with significant BLAST matches. GO enrichment analysis of Norin 10 at different CO_2 concentrations showed that some functional genes related to plastids, precursor metabolites, and energy, thylakoid and photosynthesis were apparently enriched at elevated CO_2（550 μmol mol^–1） in contrast to that at ambient CO_2（400 μmol mol^–1）; these findings were further confirmed by RT-PCR analysis. The findings demonstrated the specific effects of elevated CO_2 during long-term period in free air CO_2 enrichment（FACE） on transcriptome response of the high yielding wheat variety, Norin 10, which has a large spike.展开更多
Since its first release in 2008,Norine remains the unique resource completely devoted to nonribosomal peptides(NRPs).They are very attractive microbial secondary metabolites,displaying a remarkable diversity of struct...Since its first release in 2008,Norine remains the unique resource completely devoted to nonribosomal peptides(NRPs).They are very attractive microbial secondary metabolites,displaying a remarkable diversity of structure and functions.Norine(http://bioinfo.lifl.fr/NRP)includes a database now containing more than 1160 annotated peptides and user-friendly interfaces enabling the querying of the database,through the annotations or the structure of the peptides.Dedicated tools are associated for structural comparison of the compounds and prediction of their biological activities.In this paper,we start by describing the knowledgebase and the dedicated tools.We then present some user cases to show how useful Norine is for the discovery of novel nonribosomal peptides.展开更多
基金financial supports from the National Basic Research Program of China(973 Program,2012CB955904)the Agricultural Science and Technology Innovation Program(ASTIP)of Chinese Academy of Agricultural Sciences
文摘The increasing atmospheric carbon dioxide concentration, caused by fossil fuel combustion and deforestation, plays an important role in plant growth and development. Wheat, as a major staple crop, adapts to climate change by tuning its inherent molecular mechanism, which is not well understood. The present study employed the RNA-Seq method to generate transcriptome profiles of the wheat Norin 10 in response to elevated CO_2 in comparison with ambient CO_2. The 10 895 787 high-quality clean reads of Norin 10 were assembled de novo using Trinity（without a reference genome） resulting in a total of 18 206 candidate transcripts with significant BLAST matches. GO enrichment analysis of Norin 10 at different CO_2 concentrations showed that some functional genes related to plastids, precursor metabolites, and energy, thylakoid and photosynthesis were apparently enriched at elevated CO_2（550 μmol mol^–1） in contrast to that at ambient CO_2（400 μmol mol^–1）; these findings were further confirmed by RT-PCR analysis. The findings demonstrated the specific effects of elevated CO_2 during long-term period in free air CO_2 enrichment（FACE） on transcriptome response of the high yielding wheat variety, Norin 10, which has a large spike.
文摘Since its first release in 2008,Norine remains the unique resource completely devoted to nonribosomal peptides(NRPs).They are very attractive microbial secondary metabolites,displaying a remarkable diversity of structure and functions.Norine(http://bioinfo.lifl.fr/NRP)includes a database now containing more than 1160 annotated peptides and user-friendly interfaces enabling the querying of the database,through the annotations or the structure of the peptides.Dedicated tools are associated for structural comparison of the compounds and prediction of their biological activities.In this paper,we start by describing the knowledgebase and the dedicated tools.We then present some user cases to show how useful Norine is for the discovery of novel nonribosomal peptides.
