An actin gene （BPH-Actin3） from the important rice pest brown planthopper, Nilaparvata lugens, was cloned and gene expression was characterized at different development stages. The gene was 1461bp with an open readi...An actin gene （BPH-Actin3） from the important rice pest brown planthopper, Nilaparvata lugens, was cloned and gene expression was characterized at different development stages. The gene was 1461bp with an open reading frame of l l31bp coding for a 376 amino acids protein, with 330 nucleotides of the 3＇-untranslation region （3＇-UTR）. The amino acid sequence deduced from the nucleotide sequence showed higher similarities to other insect muscle actins （94-95%） than those to non-muscle actins （87-93%）. The 3＇-UTR contains several AU-rich elements （AREs） AUUUA/UAAAU and one extended ARE UAAAAAU, which may function in regulating mRNA decay. Northern blot and RT-PCR studies showed BPH-Actin3 expressed at brown planthopper full larval stages with the highest mRNA levels at 3rd and 4th instar stages, but not expressed at egg and adult stages. Because the 3rd and 4th instars are the key development stages for brown planthopper wing-form determination, it was thought BPH-Actin3 might play important roles in brown planthopper wing development.展开更多
文摘An actin gene （BPH-Actin3） from the important rice pest brown planthopper, Nilaparvata lugens, was cloned and gene expression was characterized at different development stages. The gene was 1461bp with an open reading frame of l l31bp coding for a 376 amino acids protein, with 330 nucleotides of the 3＇-untranslation region （3＇-UTR）. The amino acid sequence deduced from the nucleotide sequence showed higher similarities to other insect muscle actins （94-95%） than those to non-muscle actins （87-93%）. The 3＇-UTR contains several AU-rich elements （AREs） AUUUA/UAAAU and one extended ARE UAAAAAU, which may function in regulating mRNA decay. Northern blot and RT-PCR studies showed BPH-Actin3 expressed at brown planthopper full larval stages with the highest mRNA levels at 3rd and 4th instar stages, but not expressed at egg and adult stages. Because the 3rd and 4th instars are the key development stages for brown planthopper wing-form determination, it was thought BPH-Actin3 might play important roles in brown planthopper wing development.
