The genomic sequence of the attenuated hog cholera virus Lapinized Chinese strain (HCLV) was determined from overlapping cDNA clones. The viral RNA of HCLV stain comprised 12 310 nucleotide (nt) including 374 nt and 2...The genomic sequence of the attenuated hog cholera virus Lapinized Chinese strain (HCLV) was determined from overlapping cDNA clones. The viral RNA of HCLV stain comprised 12 310 nucleotide (nt) including 374 nt and 239 nt at the 5′ and 3′-noncoding region, respectively. The complete genome sequence contained one large open reading frame which encoded an amino acid sequence of 3 898 residues with a calculated molecular weight of 437×103. Although there were mostly only small differences between the sequence of the HCLV strain and the published sequences of strains ALD, GPE?, Alfort and Brescia, there was one notable insertion of 12 nucleotides, TTTTCTTTTTTC in the 3′ non-coding region of HCLV strain.展开更多
An attenuated hepatitis A virus (HAV) strain (H2 strain) has been developed successfully by Mao et al. in our laboratory, which has been proven to have a good immanogenicity in animal and human trials. The molecular b...An attenuated hepatitis A virus (HAV) strain (H2 strain) has been developed successfully by Mao et al. in our laboratory, which has been proven to have a good immanogenicity in animal and human trials. The molecular biological research for H2 strain has been carried out in order to darify the attenuation mechanism of H2 strain on the molecular level.展开更多
INTRODUCTIONHepatocellular carcinoma(HCC)is one of the major causes of death in the word.The mechanism of carcinogenesis is unknown,although it is widely accepted that HBV and HCV are clsely related to liver cancer[1-...INTRODUCTIONHepatocellular carcinoma(HCC)is one of the major causes of death in the word.The mechanism of carcinogenesis is unknown,although it is widely accepted that HBV and HCV are clsely related to liver cancer[1-5[1-5].Previously,a variety of studies have described the differences in gene expression which distinguished tumor from nontumor[6-11].Cloning of the genes,especially the genes associated with HBV and HCV,is still very important to account for the development of liver cancer.展开更多
INTRODUCTIONThe mechanism of hepatocellular carcinoma(HCC)is still unclear,although some genes have been found to play a role in the transformation of liver cells,and a variety of studies have described differences in...INTRODUCTIONThe mechanism of hepatocellular carcinoma(HCC)is still unclear,although some genes have been found to play a role in the transformation of liver cells,and a variety of studies have described differences in gene expression which distinguished tumor from nontumor[1-6].The new genes,especially the functional genes directly related with tumor are still worth being found.The purpose of our study is to find the different genes between human liver tumor and normal tissues using suppression subtractive hybridization.展开更多
A gene library of the infectious spleen and kidney necrosis virus(ISKNV) from mandarinfish,Siniperca chuatsi(Basilewsky) genome was established ISKNV DNA was cleaved with EcoRI,BamHI,HindⅢ,KpnI and PstI and the resul...A gene library of the infectious spleen and kidney necrosis virus(ISKNV) from mandarinfish,Siniperca chuatsi(Basilewsky) genome was established ISKNV DNA was cleaved with EcoRI,BamHI,HindⅢ,KpnI and PstI and the resulting fragments were inserted into the corresponding sites of the pBluescript Ⅱ KS plasmid vector using T4 DNA ligase Bacterial colonies harboring recombinant plasmids were selected All cloned fragments were individually identified by digestion of the recombinant plasmid DNA with different restriction enzymes and screened by hybridization of recombinant plasmid DNA to viral DNA Using these recombinant plasmids,the physical maps of the genome were constructed for BamHI,HindⅢ and KpnI restriction展开更多
文摘The genomic sequence of the attenuated hog cholera virus Lapinized Chinese strain (HCLV) was determined from overlapping cDNA clones. The viral RNA of HCLV stain comprised 12 310 nucleotide (nt) including 374 nt and 239 nt at the 5′ and 3′-noncoding region, respectively. The complete genome sequence contained one large open reading frame which encoded an amino acid sequence of 3 898 residues with a calculated molecular weight of 437×103. Although there were mostly only small differences between the sequence of the HCLV strain and the published sequences of strains ALD, GPE?, Alfort and Brescia, there was one notable insertion of 12 nucleotides, TTTTCTTTTTTC in the 3′ non-coding region of HCLV strain.
文摘An attenuated hepatitis A virus (HAV) strain (H2 strain) has been developed successfully by Mao et al. in our laboratory, which has been proven to have a good immanogenicity in animal and human trials. The molecular biological research for H2 strain has been carried out in order to darify the attenuation mechanism of H2 strain on the molecular level.
文摘INTRODUCTIONHepatocellular carcinoma(HCC)is one of the major causes of death in the word.The mechanism of carcinogenesis is unknown,although it is widely accepted that HBV and HCV are clsely related to liver cancer[1-5[1-5].Previously,a variety of studies have described the differences in gene expression which distinguished tumor from nontumor[6-11].Cloning of the genes,especially the genes associated with HBV and HCV,is still very important to account for the development of liver cancer.
文摘INTRODUCTIONThe mechanism of hepatocellular carcinoma(HCC)is still unclear,although some genes have been found to play a role in the transformation of liver cells,and a variety of studies have described differences in gene expression which distinguished tumor from nontumor[1-6].The new genes,especially the functional genes directly related with tumor are still worth being found.The purpose of our study is to find the different genes between human liver tumor and normal tissues using suppression subtractive hybridization.
文摘A gene library of the infectious spleen and kidney necrosis virus(ISKNV) from mandarinfish,Siniperca chuatsi(Basilewsky) genome was established ISKNV DNA was cleaved with EcoRI,BamHI,HindⅢ,KpnI and PstI and the resulting fragments were inserted into the corresponding sites of the pBluescript Ⅱ KS plasmid vector using T4 DNA ligase Bacterial colonies harboring recombinant plasmids were selected All cloned fragments were individually identified by digestion of the recombinant plasmid DNA with different restriction enzymes and screened by hybridization of recombinant plasmid DNA to viral DNA Using these recombinant plasmids,the physical maps of the genome were constructed for BamHI,HindⅢ and KpnI restriction
