Cotton(Gossypium hirsutum L.) is the most important textile fiber and the second most important oil seed source in the world.To control excessive growth of cotton plant height,which may result in shading and lodging,f...Cotton(Gossypium hirsutum L.) is the most important textile fiber and the second most important oil seed source in the world.To control excessive growth of cotton plant height,which may result in shading and lodging,farmers and researchers have used plant growth regulators that展开更多
定向诱导基因组局部突变(targeting induced local lesions in genomes,TILLING)技术将化学诱变与高通量突变检测技术相结合,可高效、快速地从突变群体中鉴定出目标基因突变位点。本文在概述TILLING技术应用于水稻、小麦、玉米、大豆等...定向诱导基因组局部突变(targeting induced local lesions in genomes,TILLING)技术将化学诱变与高通量突变检测技术相结合,可高效、快速地从突变群体中鉴定出目标基因突变位点。本文在概述TILLING技术应用于水稻、小麦、玉米、大豆等作物突变研究现状的基础上,重点综述了TILLING分析群体构建与突变位点检测方法的技术改进与发展,探讨了TILLING技术目前存在的问题与前景。展开更多
Mutants showing spontaneous cell death in the absence of pathogen attack are called lesion mimic mutants （lmm） （Lorrain et al., 2003）. These mutants usually exhibit typical hypersensitive responses （HRs） within ...Mutants showing spontaneous cell death in the absence of pathogen attack are called lesion mimic mutants （lmm） （Lorrain et al., 2003）. These mutants usually exhibit typical hypersensitive responses （HRs） within or around the lesion spots, which are frequently observed in plants challenged with avirulent pathogens （Lorrain et al., 2003）. A number of these mutants have been characterized in rice （Zeng et al., 2004）, Arabidopsis （Guo et al., 2013）, maize （Wang et al., 2013） and barley （Persson et al., 2008）. Most lmm show enhanced resistance to various pathogens （Huang et al., 2011）, because HR is usually accompanied with enhanced defense responses, such as reactive oxygen species （ROS） activation （Qiao et al., 2010） and increased expression of pathogenesis-related genes （Lorrain et al., 2003）. Additionally, most lmm exhibit defects in growth and development due to the disordered physiolog- ical and molecular processes caused by the lesion spots. Thus, lmm are powerful tools for the study of the molecular mech- anisms of cell death, plant development and disease resistance （Lorrain et al., 2003; Babu et al., 2011）.展开更多
文摘Cotton(Gossypium hirsutum L.) is the most important textile fiber and the second most important oil seed source in the world.To control excessive growth of cotton plant height,which may result in shading and lodging,farmers and researchers have used plant growth regulators that
文摘定向诱导基因组局部突变(targeting induced local lesions in genomes,TILLING)技术将化学诱变与高通量突变检测技术相结合,可高效、快速地从突变群体中鉴定出目标基因突变位点。本文在概述TILLING技术应用于水稻、小麦、玉米、大豆等作物突变研究现状的基础上,重点综述了TILLING分析群体构建与突变位点检测方法的技术改进与发展,探讨了TILLING技术目前存在的问题与前景。
基金supported by grants from the National Natural Science Foundation of China No. 31401351 to J. Wang, and No. 31171622 and No. 31371705 to X. W. Chensupported by the "Hundred Talents Plan" Foundation of Sichuan to X. Chenthe Specialized Research Fund for Doctoral Program of Higher Education (No. 20135103120004) to J. Wang
文摘Mutants showing spontaneous cell death in the absence of pathogen attack are called lesion mimic mutants （lmm） （Lorrain et al., 2003）. These mutants usually exhibit typical hypersensitive responses （HRs） within or around the lesion spots, which are frequently observed in plants challenged with avirulent pathogens （Lorrain et al., 2003）. A number of these mutants have been characterized in rice （Zeng et al., 2004）, Arabidopsis （Guo et al., 2013）, maize （Wang et al., 2013） and barley （Persson et al., 2008）. Most lmm show enhanced resistance to various pathogens （Huang et al., 2011）, because HR is usually accompanied with enhanced defense responses, such as reactive oxygen species （ROS） activation （Qiao et al., 2010） and increased expression of pathogenesis-related genes （Lorrain et al., 2003）. Additionally, most lmm exhibit defects in growth and development due to the disordered physiolog- ical and molecular processes caused by the lesion spots. Thus, lmm are powerful tools for the study of the molecular mech- anisms of cell death, plant development and disease resistance （Lorrain et al., 2003; Babu et al., 2011）.
