Procyanidin A_1 and its digestive products alleviate acrylamide-induced IPEC-J2 cell damage through regulating Keap1/Nrf2 pathway

Our previous study has revealed that procyanidin A_(1)(A_(1))and its simulated digestive product(D-A,)can alleviate acrylamide(ACR)-induced intestine cell damage.However,the underlying mechanism remains unknown.In this study,we elucidated the molecular mechanism for and D-A_(1) to alleviate ACR-stimulated IPEC-J2 cell damage.ACR slightly activated nuclear factor erythroid 2-related factor 2(Nrf2)signaling and its target genes,but this activation could not reduce intestine cell damage.A_(1) and D-A_(1) could alleviate ACR-induced cell damage,but the effect was abrogated in cells transiently transfected with Nrf2 small interfering RNA(siRNA).Further investigation confirmed that A_(1) and D-A_(1) interacted with Ketch-like ECH-associated protein 1(Keapl),which boosted the stabilization of Nrf2,subsequently promoted the translocation of Nrf2 into the nucleus,and further increased the expression of antioxidant proteins,thereby inhibiting glutathione(GSH)consumption,maintaining redox balance and eventually alleviating ACR-induced cell damage.Importantly,there was no difference between A_(1) and D-A_(1) treated groups,indicating that A_(1) can tolerate gastrointestinal digestion and may be a potential compound to limit the toxicity of ACR.

Phosphatidylglycerol-containing ER-transport vesicles built and restore outer mitochondrial membrane and deliver nuclear DNA translation products to generate cardiolipin in the inner mitochondrial membrane

Phosphatidylglycerol (PG) an important membrane phospholipid required for the synthesis of diphos-phatidylglycerol (DPG) commonly known as cardiolipin (CL) was identified in the fraction of endo-plasmic reticulum (ER)-derived transport vesicles which had no affinity for Golgi. The vesicles were produced in the presence of Brefeldin A (BFA), the agent known to inhibit ER-Golgi transport, and found to display affinity to mitochondria. The analysis revealed that their cargo was not containing proteins that are transported to Golgi, and that their membrane was free of phosphatidylinositol (PI) and ceramides (Cer). The incubation of PG-containing transport vesicles with mitochondria afforded incorporation of their membrane into the Outer Mito-chondrial Membrane (OMM) and formation of lyso-phosphatidylglycerol (LPG). In turn, upon further incubation with fresh transport active cytosol, the mitochondrial LPG was converted to PG. The results of analysis of the OMM, Inner Mitochondrial Mem-brane (IMM) and Inner Mitochondrial Space Components (IMSC) strongly suggest that PG-containing transport vesicles deliver nuclear DNA translation products to the IMSC and thus facilitate CL synthesis in the IMM. In summary, our studies provide evidence that ER-generated PG-enriched transport vesicles represent the general pathway for restitution of mitochondrial membranes and the delivery of nuclear DNA translation products that generate CL, and thus sustain the mitochondrial matrix CL-dependent metabolic reactions.

Autophagy plays a protective role in advanced glycation end products-induced apoptosis of chondrocytes via regulation of tumor necrosis factor-α,nuclear factor-κ B and reactive oxygen species

Objective: To study the adverse effects of advanced glycation end products(AGEs) on chondrocytes and the role of autophagy in this process. Methods: Chondrocytes were harvested from the human articular cartilage tissues in surgery. AGEs were administered during chondrocytes culture. The rapamycin was used to induce autophagy. The cell viability was determined by 3-[4,5-dimethylthiazol2-yl]-2,5-diphenyl tetrazolium bromide(MTT) assay.The expression of tumor necrosis factor-α(TNF-α) and nuclear factor-κ B(NF-κ B) was detected by quantitative real-time polymerase chain reaction. The reactive oxygen species(ROS) production and apoptosis of the chondrocytes were determined by fluorescent probe and flow cytometer, respectively. Results: The chondrocytes viability was significantly reduced after 12 h incubation with AGEs(P<0.01)). In contrast, rapamycin pretreatment increased the chondrocytes viability through autophagy. AGEs increased TNF-α and NF-κ B mRNA expression of chondrocytes and autophagy receded or proceeded the change. AGEs increased intracellular ROS accumulation and autophagy reversed the change. AGEs accelerated chondrocytes apoptosis and autophagy suspended apoptosis. Conclusions: Accumulation of AGEs may have an adverse role for chondrocytes by increasing TNF-α and NF-κB expression, ROS accumulation and apoptosis; meanwhile, autophagy ameliorates the AGEsinduced adverse effects.

Numerical analysis on element creation by nuclear transmutation of fission products

A burnup calculation was performed to analyze the Apr`es ORIENT process, which aims to create highlyvaluable elements from fission products separated from spent nuclear fuels. The basic idea is to use nuclear transmutation induced by a neutron capture reaction followed by a β-decay, thus changing the atomic number Z of a target element in fission products by 1 unit. LWR(PWR) and FBR(MONJU) were considered as the transmutation devices. High rates of creation were obtained in some cases of platinum group metals(44Ru by FBR,46 Pd by LWR) and rare earth(64Gd by LWR,66 Dy by FBR). Therefore, systems based on LWR and FBR have their own advantages depending on target elements. Furthermore, it was found that creation rates of even Z(= Z + 1) elements from odd Z ones were higher than the opposite cases. This creation rate of an element was interpreted in terms of "average 1-group neutron capture cross section of the corresponding target element σc Z defined in this work. General trends of the creation rate of an even(odd) Z element from the corresponding odd(even) Z one were found to be proportional to the 0.78th(0.63th) power of σc Z, however with noticeable dispersion. The difference in the powers in the above analysis was explained by the difference in the number of stable isotopes caused by the even-odd effect of Z.

AN IMPROVED MODEL FOR COMPUTING SOLUTION DYNAMICS OF NATURAL PRODUCTS WITH ^(13)C NUCLEAR MAGNETIC RELAXATION

The fully anisotropic molecular overall tumbling model with methyl conformation jumps internal rotation among three equivalent sites is proposed,the overall tumbling rotation rates and the methyl internal rotation rates of ponicidin are computed with this model from ~C relaxation parameters.

Nuclear dynamics and particle production near threshold energies in heavy-ion collisions

Recent progress of the quantum molecular dynamics model for describing the dynamics of heavy-ion collisions is viewed, in particular the nuclear fragmentation, isospin physics, particle production and in-medium effect, hadron-induced nuclear reactions, hypernucleus, etc.The neck fragmentation in Fermi-energy heavy-ion collisions is investigated for extracting the symmetry energy at subsaturation densities. The isospin effects, in-medium properties, and the behavior of high-density symmetry energy in medium-and high-energy heavy-ion collisions are thoroughly discussed. The hypernuclide dynamics formed in heavy-ion collisions and in hadron-induced reactions is analyzed and addressed in the future experiments at the high-intensity heavy-ion accelerator facility(HIAF).

High glucose reduces Nrf2-dependent cRAGE release and enhances inflammasome-dependent IL-1βproduction in monocytes:the modulatory effects of EGCG

Soluble receptor for advanced glycation end products(sRAGE)acts as a decoy sequestering of RAGE ligands,thus preventing the activation of the ligand-RAGE axis linking human diseases.However,the molecular mechanisms underlying sRAGE remain unclear.In this study,THP-1 monocytes were cultured in normal glucose(NG,5.5 mmol/L)and high glucose(HG,15 mmol/L)to investigate the effects of diabetesrelevant glucose concentrations on sRAGE and interleukin-1β(IL-1β)secretion.The modulatory effects of epigallocatechin gallate(EGCG)in response to HG challenge were also evaluated.HG enhanced intracellular reactive oxygen species(ROS)generation and RAGE expression.The secretion of sRAGE,including esRAGE and cRAGE,was reduced under HG conditions,together with the downregulation of a disintegrin and metallopeptidase 10(ADAM10)and nuclear factor erythroid 2-related factor 2(Nrf2)nuclear translocation.Mechanistically,the HG effects were counteracted by siRAGE and exacerbated by siNrf2.Chromatin immunoprecipitation results showed that Nrf2 binding to the ADAM10 promoter and HG interfered with this binding.Our data reinforce the notion that RAGE and Nrf2 might be sRAGE-regulating factors.Under HG conditions,the treatment of EGCG reduced ROS generation and RAGE activation.EGCG-stimulated cRAGE release was likely caused by the upregulation of the Nrf2-ADAM10 pathway.EGCG inhibited HG-mediated NLRP3 inflammasome activation at least partly by stimulating sRAGE,thereby reducing IL-1βrelease.

Experiment of dynamic seepage of tight/shale oil under matrix fracture coupling

A physical simulation method with a combination of dynamic displacement and imbibition was established by integrating nuclear magnetic resonance(NMR)and CT scanning.The microscopic production mechanism of tight/shale oil in pore throat by dynamic imbibition and the influencing factors on the development effect of dynamic imbibition were analyzed.The dynamic seepage process of fracking-soaking-backflow-production integration was simulated,which reveals the dynamic production characteristics at different development stages and their contribution to enhancing oil recovery(EOR).The seepage of tight/shale reservoirs can be divided into three stages:strong displacement and weak imbibition as oil produced rapidly by displacement from macropores and fractures,weak displacement and strong imbibition as oil produced slowly by reverse imbibition from small pores,and weak displacement and weak imbibition at dynamic equilibrium.The greater displacement pressure results in the higher displacement recovery and the lower imbibition recovery.However,if the displacement pressure is too high,the injected water is easy to break through the front and reduce the recovery degree.The higher the permeability,the greater the imbibition and displacement recovery,the shorter the time of imbibition balance,and the higher the final recovery.The fractures can effectively increase the imbibition contact area between matrix and water,reduce the oil-water seepage resistance,promote the oil-water displacement between matrix and fracture,and improve the oil displacement rate and recovery of the matrix.The soaking after fracturing is beneficial to the imbibition replacement and energy storage of the fluid;also,the effective use of the carrying of the backflow fluid and the displacement in the mining stage is the key to enhancing oil recovery.

Sigma Meson Production in Nuclear Reactions

The sigma meson production in p ＋ ^12C and p A- ^40Ca reactions at the incident energy Ep = 1.5 GeV is investigated within the Quantum Molecular Dynamics model. The simulation results indicate a distinctive A dependence of the sigma production, that is, the increase of A is followed by an increase of the production cross sections. We find that the σ meson production in proton-induced reactions is strongly medium-dependent, and the produced σ mesons decaying in a denser medium experience a stronger mass shift towards lower masses. This mass shift is an experimentally accessible observable in the final state pion pairs, which do not suffer from reabsorption by the surrounding nucleons. It is pointed out that the ratio of measured sigma cross sections as a function of the sigma invariant-mass from various reactions is a good probe to explore the existence of the σ meson in a dense nuclear environment.

Studies on Capacity Expansion of Fuel Plants for Nuclear Research Reactors

The demand for nuclear fuel for research reactors is rising worldwide. Thus, the production facilities of this kind of fuel need reliable guidance on how to augment their production in order to meet the increasing demand efficiently and safely. We proposed a specific procedure for increasing production capacity. That procedure was tested with data from a real plant, which produces plate-type fuel elements loaded with LEU U3Si2-Al fuel. The test was made by means of discrete event simulation, and the results indicated the proposed procedure is efficient in raising production capacity.

Rapidity and Pseudorapidity Distributions of theVarious Hadron-Species Produced in High EnergyNuclear Collisions: A Systematic Approach

With the help of a phenomenological approach outlined in the text in some detail, we have dealt here with the description of the plots on rapidity and pseudorapidity spectra of some hadron-secondaries produced in various nucleus-nucleus interactions at high energies. The agreement between the measured data and the attempted fits are, on the whole, modestly satisfactory excepting a very narrow central region in the vicinity of y = η = 0. At last, hints to how the steps suggested in the main body of the text to proceed with the description of the measured data given in the plots could lead finally to a somewhat systematic methodology have also been made.

Entropy Production and Fractal Dimensions in Heavy Ion Nuclear Reaction at Intermediate Energies

The characteristics of the nonlinear dynamics in the Heavy Ion Collision (HIC) at intermediate energies have been studied by evaluating the productions of the Generalized Entropy (GE) and the Multifragmentation Entropy (ME) as well as the features of the information and fractal dimensions within the Isospin Quantum Molecular Dynamical Model compensated by the lattice methods. Results demonstrate from various views that the existence of deterministic chaos in the dynamical process of reaction.

Study on efficiency of DCP for nuclear hydrogen production

With many advantages, hydrogen is considered as the fuel of the future. But there is no natural resourceof hydrogen and it must be produced by other kinds of energy. As for the primary energy, nuclear energy is a promis-ingalternative. Using heat from nuclear reactor to produce hydrogen is receiving more and more concerns in recentyears. This paper mainly emphasizes the study of the direct contact pyrolysis (DCP) of methane using heat from nu-clearreactor. A facility was designed to investigate the efficiency of DCP process in certain conditions. The experi-mentalresults show that this process produces only hydrogen and carbon. The conversion efficiency increases withtemperature and residence time, but decreases as flow rate increases. The highest efficiency of DCP obtained in thisexperiment is about 22%.

Nuclear magnetic resonance study of the formation and dissociation process of nature gas hydrate in sandstone

In this work,the authors monitored the formation and dissociation process of methane hydrate in four different rock core samples through nuclear magnetic resonance(NMR)relaxation time(T_(2))and 2D imaging measurement.The result shows that the intensity of T_(2) spectra and magnetic resonance imaging(MRI)signals gradually decreases in the hydrate formation process,and at the same time,the T_(2) spectra move toward the left domain as the growth of hydrate in the pores of the sample accelerates the decay rate.The hydrate grows and dissociates preferentially in the purer sandstone samples with larger pore size and higher porosity.Significantly,for the sample with lower porosity and higher argillaceous content,the intensity of the T_(2) spectra also shows a trend of a great decrease in the hydrate formation process,which means that high-saturation gas hydrate can also be formed in the sample with higher argillaceous content.The changes in MRI of the sample in the process show that the formation and dissociation of methane hydrate can reshape the distribution of water in the pores.

Productions of kaon and lambda by 50 GeV protons incident on nuclear targets

A Glauber multiple scattering theory in terms of pations and nucleons is applied to study productions of kaon, lambda and antilambda ill 50 GeV proton-nucleus scatterings at JHF (the Japan Hadron Facility). Tile ratios of tile differential cross sections of nucleontungsten to nucleon-beryllium are calclutated versus the transverse momentum at central rapidity. The cancellation between the single and double scattering amplitudes leads to nuclear enhancement of strangeness production at central rapidity.

Nuclear Effect on Gluon Distribution Function and Associate Production of J/Ψ and y with Large P_T in High Energy p-Fe Collision

With three theoretical models of nuclear effects on gluon distribution functions,the differentialcross sections and the total cross sections for associate production of J/ψ and γ with large P_T in high energyp-Fe collisions are calculated.

New developments at nuclear emulsion experiment

Three new nuclear cmulsion techniques used for measuring emission angles ofcharged particles in central events at ultra-high evergy heavy-ion collisions are fully described.

Studies on Production Planning of Dispersion Type U3Si2-Al Fuel in Plate-Type Fuel Elements for Nuclear Research Reactors

Several fuel plants that supply nuclear research reactors need to increase their production capacity in order to meet the growing demand for this kind of nuclear fuel. After the enlargement of the production capacity of such plants, there will be the need of managing the new production level. That level is usually the industrial one, which poses challenges to the managerial staff. Such challenges come from the fact that several of those plants operate today on a laboratorial basis and do not carry inventory. The change to the industrial production pace asks for new actions regarding planning and control. The production process based on the hydrolysis of UF6 is not a frequent production route for nuclear fuel. Production planning and control of the industrial level of fuel production on that production route is a new field of studies. The approach of the paper consists in the creation of a mathematical linear model for minimization of costs. We also carried out a sensitivity analysis of the model. The results help in minimizing costs in different production schemes and show the need of inventory. The mathematical model is dynamic, so that it issues better results if performed monthly. The management team will therefore have a clearer view of the costs and of the new, necessary production and inventory levels.

Comissioning of the lEA-R1 Nuclear Reactor New Heat Exchanger

This work presents results on the commissioning of the new heat exchanger of the IEA-R1 nuclear reactor in the occasion of its operational power upgrade from 2 MW to 5 MW, in comparison to the values calculated in the project of IESA Design and Equipments Company. This reactor is a swimming pool type, light water moderated and with graphite reflectors, used for research purposes and medical radioisotopes production. During monitoring procedures, issues were observed on the reactor operation at 5 MW mainly due to the ageing of the reactor＇s oldest heat exchanger （TC-A） and excessive vibrations at high flow rates on the other installed heat exchanger （TC-B）. So it was decided to provide a new IESA heat exchanger with 5 MW capacity to definitely substitute the TC-A heat exchanger. The results show that the IEA-R1 nuclear reactor can be operated safely and continuously at 5 MW with the new IESA heat exchanger.

A method of analysing experimental data of nuclear reaction cross sections

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