Influence of norcantharidin on proliferation,proliferation-related gene proteins prolifera-ting cell nuclear antigen and Ki-67 of human gallbladder carcinoma GBC-SD cells

BACKGROUND: Gallbladder carcinoma is a highly lethal and aggressive disease with early metastasis, strong invasion and poor prognosis. Most patients with this disease are at the advanced and un-resectable stage and should be consi- dered for palliative treatment such as chemotherapy and ra- diotherapy. Unfortunately, reports of chemotherapy and radiotherapy for gallbladder carcinoma are disappointing. We investigated the influence of norcantharidin (NCTD) on proliferation, proliferation-related gene proteins PCNA and Ki-67 of human gallbladder carcinoma GBC-SD cells in vitro. METHODS: GBC-SD cell lines of human gallbladder carci- noma were cultured by the cell culture technique. The ex- periment was divided into NCTD group and control group. The tetrazolium-based colorimetric assay was used to evaluate cell growth. The streptavidin-biotin complex method was used to determine the expressions of prolifera- tion-related gene proteins PCNA and Ki-67 of human gall- bladder carcinoma GBC-SD cells. RESULTS: NCTD inhibited the growth and proliferation of GBC-SD cells from 10 mg/L or after 6 hours in a dose- and time-dependent manner, with the IC50 value of 56.18 μg/ ml at 48 hours. After treatment with NCTD, the expression of PCNA (0.932 ±0.031 vs. 0.318 ±0.023, P<0.001) and Ki-67 (0.964 ±0.092 vs. 0.297 ±0.018, P<0.001) proteins were decreased significantly. CONCLUSION: NCTD inhibits the proliferation of human gallbladder carcinoma GBC-SD cells in vitro and the expres- sion of their proliferation-related gene proteins PCNA and Ki-67.

Expression of COX-2, PCNA, Ki-67 and p53 in gastrointestinal stromal tumors and its relationship with histopathological parameters

AIM: To investigate the expression of Cyclooxygenase-2 (COX-2), proliferating cell nuclear antigen (PCNA), Ki-67 and p53 in gastrointestinal stromal tumors (GISTs) and its relationship with histopathological parameters. METHODS: Twenty-five GISTs were examined by light microscopy and immunohistochemistry. c-kit, CD34, SMA, S-100 protein, COX-2, PCNA, Ki-67 and p53 were detected immunohistochemically and the relationship was evaluated among histopathologic parameters such as mitotic index (MI), tumor grade, tumor size, COX-2, PCNA, Ki-67 and p53. RESULTS: COX-2 protein expression was found in 19 of 25 (76%) of the tumors, and expression was noted in the cytoplasm of the tumor cells. p53 was significantly related to MI and tumor grade but no relationship was found between COX-2, proliferation markers and MI, tumor grade and tumor size. CONCLUSION: COX-2 is expressed in most GISTs and it may play an important role in the proliferation and progression of these tumors or a useful marker to identify GIST. Although immunohistochemical assessment of p53 can be used for distinguishing the risk groups of GISTs, tumor size and mitotic rate should be considered at the same time.

The predictive value of vascular endothelial growth factor and Ki-67 expression on neoadjuvant therapy in rectal cancer

Objective： To investigate the expressions of vascular endothelial growth factor （VEGF） and proliferating cell nuclear antigen （Ki-67） in patients with rectal adenocarcinoma and their associations with neoadjuvant therapy. Methods： The expressions of Ki-67 and VEGF in 32 cases of rectal adenocarcinoma, including both pretreatment tumor biopsies and postoperative specimen, were detected by immunohistochemistry using specific antibodies, and were correlated with clinicopathological factors. Results： The intensity of VEGF staining was significantly correlated with lymph nodal metastasis （P =0.033）, depth of tumor invasion （P =0.007） and tumor stage （P= 0.016）, but not with histological types, tumor sizes, patients＇ ages and genders （P 〉 0.05）. Low level of VEGF expression had significant correlation with the high sensitivity of response to neoadjuvant therapy （P = 0.016）. The transient increase of VEGF expression could be seen after neoadjuvant therapy （P = 0.035）. Ki-67 labeling index （Ki-67-LI） was significantly correlated with lymph node metastasis （P = 0.028）, but not correlated to tumor sizes, patients＇ ages and genders （P 〉 0.05）. Tumors with lower Ki-67-LI were more sensitive to neoadjuvant therapy （P = 0.032）. The Ki-67 level decreased after neoadjuvant therapy, but no statistical significance was found （P 〉 0.05）. Conclusion： Our results demonstrate that the expression of VEGF and Ki-67 in pretreatment rectal adenocarcinoma biopsies may be predictive of tumor response to neoadjuvant therapy.

细胞核抗原抗体Ki-67及趋化因子受体4与甲状腺癌患者相关病理学参数的关联性分析

目的分析细胞核抗原抗体Ki-67、趋化因子受体4(CXCR4)与甲状腺癌(TC)患者相关病理学参数的关联性。方法选取2016年5月至2019年4月本院72例TC组织标本为TC组,另选取同期36例甲状腺肿组织标本为良性组。以免疫组织化学法测定两组标本Ki-67、CXCR4表达情况,分析其表达与TC病理学特征的关联性。结果TC组Ki-67、CXCR4阳性表达率分别为69.44%(50/72)、76.39%(55/72),均高于良性组5.56%(2/36)、33.33%(12/36),差异均有统计学意义(均P<0.05);Ⅲ~Ⅳ期TC患者Ki-67阳性表达率87.88%(29/33),高于Ⅰ~Ⅱ期53.85%(21/39),淋巴结转移TC患者Ki-67阳性表达率84.38%(27/32),高于非淋巴结转移TC患者57.50%(23/40),Ⅲ~Ⅳ期TC患者CXCR4阳性表达率93.94%(31/33),高于Ⅰ~Ⅱ期61.54%(24/39),淋巴结转移TC患者CXCR4阳性表达率96.88%(31/32),高于非淋巴结转移TC患者60.00%(24/40),差异均有统计学意义(均P<0.05)。结论Ki-67、CXCR4在TC中阳性表达率高,均与TC临床分期、淋巴结转移有密切关系,是诊断TC患者病情的重要指标,有利于指导临床治疗、评估预后。

颅内良性脑膜瘤全切术后复发的临床病理特征及血管内皮生长因子、细胞周期相关核抗原Ki-67抗体和p53的表达分析

目的探讨肿瘤全切术后颅内良性脑膜瘤复发的临床病理特征及血管内皮生长因子(VEGF)、细胞周期相关核抗原Ki-67抗体(MIB-1)和p53表达分析。方法选取2007年1月至2018年1月间南宁市第一人民医院收治的119例行手术治疗的颅内良性脑膜瘤患者瘤体标本,记录患者性别、年龄、肿瘤位置、肿瘤直径、边界、瘤周水肿、肿瘤现状和病理类型等临床资料,采用免疫组化染色法检测VEGF、MIB-1及p53蛋白表达,观察上述资料与良性脑膜瘤复发的相关性。结果随访中患者复发23例(19. 3%)。复发和未复发患者性别、年龄、体质量指数、肿瘤部位、肿瘤直径、肿瘤形状、肿瘤边界、病理学类型及Simpson分级比较,差异均有统计学意义(均P <0. 05),瘤周水肿无统计学差异(P> 0. 05)。复发患者VEGF阳性率和MIB-1表达高于未复发患者,差异均有统计学意义(均P <0. 05),两者p53蛋白表达,差异无统计学意义(P> 0. 05)。结论良性脑膜瘤全切术后复发率较高,复发除与一般临床特征、肿瘤特征及手术切除等有关外,与血管生存和细胞增殖活跃程度相关,与p53蛋白表达无关。

Effect of preoperative transcatheter arterial chemoembolization on proliferation of hepatocellular carcinoma cells

AIM： To evaluate the effect of preoperative transcatheter arterial chemoembolization （TACE） on proliferation of hepatocellular carcinoma （HCC） cells. METHODS： A total of 136 patients with HCC underwent liver resection. Of 136 patients, 79 patients received 1 to 5 courses of TACE prior to liver resection （TACE group）, who were further subdivided into four groups： Group A （n = 11） who received i to 4 courses of chemotherapy alone; Group B （n = 33） who received i to 5 courses of chemotherapy combined with iodized oil; Group C （n = 23） who received i to 3 courses of chemotherapy combined with iodized oil and gelatin sponge; and Group D （n = 12） who received 1 to 3 courses of chemotherapy combined with iodized oil, ethanol and gelatin sponge. The other 57 patients only received liver resection （non- TACE group）. The expressions of Ki-67 and proliferating cell nuclear antigen （PCNA） protein were detected in the liver cancer tissues by immunohistochemical method. RESULTS： The Ki-67 protein expression was significantly lower in Groups C and D as compared with non-TACE group （31.35% ± 10.85% vs 44.43% ± 20.70%, 30.93% ± 18.10% vs 44.43% ± 20.70%, respectively, P 〈 0.05）. The PCNA protein expression was significantly lower in Groups C and D as compared with non-TACE group （49.61% ± 15.11% vs 62.92% ± 17.21%, 41.16% ± 11.83% vs 62.92% ± 17.21%, respectively, P 〈 0.05）. The Ki-67 protein expression was significantly higher in Group A as compared with non-TACE group （55.44% ± 13.72% vs 44.43% ± 20.70%, P 〈 0.05）. The PCNA protein expression was significantly higher in Groups A and B as compared with non-TACE group （72.22% ± 8.71% vs 62.92% ± 17.21%, 69.91% ± 13.38% vs 62.92% ± 17.21%, respectively, P 〈0.05）. CONCLUSION： Preoperative multi-material TACE suppresses the proliferation of HCC cells, while a single material embolization and chemotherapy alone enhance the proliferation of HCC cells.

Molecular biomarkers of cell proliferation in ameloblastomas

Cell proliferation is a vital biological process that is important for all living organisms because of its role in growth and the maintenance of tissue homeostasis.The control of this important process differs greatly among benign and malignant neoplasms,and the evaluation of cell proliferation in neoplasms has become a common tool used by pathologists to provide useful information pertaining to diagnosis,clinical behavior,and treatment.The usefulness of information regarding cell proliferation has led to numerous studies on the value of these methods for diagnosing different types of tumors and for clinical decision making.Ameloblastomas are no exception.This review discusses the use of several classical molecular proliferation markers,including Ki-67,proliferating cell nuclear antigen,cyclin D1 and DNA topoisomeraseⅡalpha,to characterize ameloblastomas and proposes the use of new proliferation markers used previously to characterize other neoplasms.The use of these biomark-ers offers valuable opportunities to evaluate the biological behavior of this type of odontogenic tumor.

先天性胰胆管合流异常与胆囊癌的关系

目的 从细胞增殖活性的角度了解胰胆管合流异常 (AAPB)和胆囊癌的关系。方法 ABC免疫组化法。结果 增殖细胞核抗原 (PCNA)和单克隆抗体Ki 6 7阳性细胞在AAPB合并胆囊癌的非癌部 (B组 )为 (9 2± 3 8) % ,高于不伴AAPB的胆囊癌非癌部 [D组 ,(6 6± 5 ) % ],明显高于单纯的慢性胆囊炎组 [E组 ,(1 8± 1 0 % ) ];另外 ,不伴有其他胆道疾患的AAPB(C组 )的上皮细胞增殖率[(7 4± 2 7) % ],也明显高于单纯的慢性胆囊炎组。结论 胆囊上皮细胞增殖活性在有AAPB时明显高于无AAPB者 ,此与慢性胆囊炎的存在无关。这一增殖活性的增高可能与上皮恶性变密切相关 。