Taxodiorneria peizhongii Z. J. Ye, J. J. Zhang et S. H. Pan was regarded as a new Intergenerlc hybrid between Taxodlum mucronatum Tenore （as the female donor） and Cryptomeria fortunei Hoolbrenk ex Otto et Dletr （as...Taxodiorneria peizhongii Z. J. Ye, J. J. Zhang et S. H. Pan was regarded as a new Intergenerlc hybrid between Taxodlum mucronatum Tenore （as the female donor） and Cryptomeria fortunei Hoolbrenk ex Otto et Dletr （as the male donor）. To confirm the authenticity of the intergeneric hybrid, we analyzed the rbcL gene and the Internal transcribed spacer （ITS） of 26S-18S ribosomal RNA gene of the three species using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and arbitrarily primed PCR （AP-PCR）, and ob- tained the following results：i） Taxodiomeria peizhongii had the same RFLP maps of the rbcL gene and the ITS as Taxodlum mucronatum, but was different from C. fortunei; II） a 311-bp PCR amplification product was obtained In C. fortunei by AP-PCR of ITS, but was not found in Taxodiomeria peizhongii. Our results have demonstrated that C. fortunei did not provide any genome for Taxodiomeria peizhongii, Implying that T. peizhongii Is not an Intergenerlc hybrid between the two species.展开更多
基金National Natural Science Foundation of China （39970061）.
文摘Taxodiorneria peizhongii Z. J. Ye, J. J. Zhang et S. H. Pan was regarded as a new Intergenerlc hybrid between Taxodlum mucronatum Tenore （as the female donor） and Cryptomeria fortunei Hoolbrenk ex Otto et Dletr （as the male donor）. To confirm the authenticity of the intergeneric hybrid, we analyzed the rbcL gene and the Internal transcribed spacer （ITS） of 26S-18S ribosomal RNA gene of the three species using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and arbitrarily primed PCR （AP-PCR）, and ob- tained the following results：i） Taxodiomeria peizhongii had the same RFLP maps of the rbcL gene and the ITS as Taxodlum mucronatum, but was different from C. fortunei; II） a 311-bp PCR amplification product was obtained In C. fortunei by AP-PCR of ITS, but was not found in Taxodiomeria peizhongii. Our results have demonstrated that C. fortunei did not provide any genome for Taxodiomeria peizhongii, Implying that T. peizhongii Is not an Intergenerlc hybrid between the two species.
