A 13.4 MeV deuteron beam from the 1.2 meter cyclotron of Sichuan University was used to determine theprotein content in wheat seeds on the basis of (d,p) reactions. The influence of the variation of the water content ...A 13.4 MeV deuteron beam from the 1.2 meter cyclotron of Sichuan University was used to determine theprotein content in wheat seeds on the basis of (d,p) reactions. The influence of the variation of the water content inseeds has been investigated.展开更多
The formation of·CCl3 radicals in liver nuclei was suggested by spin trapping of them with N-t-butyl-α-phenylnitrone followed by GC/MS detection of the resulting adduct. Comparison of its formation in microsomal...The formation of·CCl3 radicals in liver nuclei was suggested by spin trapping of them with N-t-butyl-α-phenylnitrone followed by GC/MS detection of the resulting adduct. Comparison of its formation in microsomal biotransformation of CCl4 was made. In aerobic nuclear activation mixtures containing NADPH and CCl4, significant decrease in the arachidonic acid content of nuclear lipids was observed (27. 8%, compared to control), the intensity of this decrease was lower than that occurring in the corresponding microsomal incubation mixtures (29.1%). Significant decreases in arachidonic acid content of nuclear and endoplasmic reticulum lipids were also observed in animals at 6 hours of poisoning with the haloalkane. During aerobic nuclear metabolism of CCl4 or CBrCl3, cholesterol oxidation products were detected: a ketocholesterol, an epoxide like structure and 7-ketocholesterol. Nuclear protein carbonyl formation was not promoted during nuclear CCl4 biotransformation. NADPH by itself may lead to protein carbonyl formation during prolonged periods of incubation. CBrCl3 in contrast, led to decreased protein carbonyl formation. No increase in nuclear protein carbonyl formation was observed in CCl4 intoxicated animals during periods of time between 1 to 6 hours after treatment. The results indicate that during nuclear biotransformation of CCl4 or CBrCl3 reactive free radicals, PUFA degradation, reactive aldehydes and cholesterol oxidation products are formed, nearby DNA and regulatory proteins.展开更多
The rates of 14 N/ 12 /C in wheat seeds wene measured by using 14 N( d,p ) 15 N and 12 C( d,p) 13 C reactions induced by 13.4MeV deuterons which wene produced by a cylotron.According to the measured 14 N/ 12 C rates,t...The rates of 14 N/ 12 /C in wheat seeds wene measured by using 14 N( d,p ) 15 N and 12 C( d,p) 13 C reactions induced by 13.4MeV deuterons which wene produced by a cylotron.According to the measured 14 N/ 12 C rates,the relative protein contents in different wheat sorts were defermined and compared with the results obtained by the Kjeldahl method.展开更多
文摘A 13.4 MeV deuteron beam from the 1.2 meter cyclotron of Sichuan University was used to determine theprotein content in wheat seeds on the basis of (d,p) reactions. The influence of the variation of the water content inseeds has been investigated.
文摘The formation of·CCl3 radicals in liver nuclei was suggested by spin trapping of them with N-t-butyl-α-phenylnitrone followed by GC/MS detection of the resulting adduct. Comparison of its formation in microsomal biotransformation of CCl4 was made. In aerobic nuclear activation mixtures containing NADPH and CCl4, significant decrease in the arachidonic acid content of nuclear lipids was observed (27. 8%, compared to control), the intensity of this decrease was lower than that occurring in the corresponding microsomal incubation mixtures (29.1%). Significant decreases in arachidonic acid content of nuclear and endoplasmic reticulum lipids were also observed in animals at 6 hours of poisoning with the haloalkane. During aerobic nuclear metabolism of CCl4 or CBrCl3, cholesterol oxidation products were detected: a ketocholesterol, an epoxide like structure and 7-ketocholesterol. Nuclear protein carbonyl formation was not promoted during nuclear CCl4 biotransformation. NADPH by itself may lead to protein carbonyl formation during prolonged periods of incubation. CBrCl3 in contrast, led to decreased protein carbonyl formation. No increase in nuclear protein carbonyl formation was observed in CCl4 intoxicated animals during periods of time between 1 to 6 hours after treatment. The results indicate that during nuclear biotransformation of CCl4 or CBrCl3 reactive free radicals, PUFA degradation, reactive aldehydes and cholesterol oxidation products are formed, nearby DNA and regulatory proteins.
文摘The rates of 14 N/ 12 /C in wheat seeds wene measured by using 14 N( d,p ) 15 N and 12 C( d,p) 13 C reactions induced by 13.4MeV deuterons which wene produced by a cylotron.According to the measured 14 N/ 12 C rates,the relative protein contents in different wheat sorts were defermined and compared with the results obtained by the Kjeldahl method.
