It is critical to establish a direct and precise method with a high sensitivity and selectivity in analytical chemistry. In this research, making use of a well known phenomenon of capillary flow, we have proposed an...It is critical to establish a direct and precise method with a high sensitivity and selectivity in analytical chemistry. In this research, making use of a well known phenomenon of capillary flow, we have proposed an image analysis method of nucleic acids at the price of a small amount of sample. When a droplet of the supramolecular complex solution, formed by neutral red and nucleic acids(NA) under an approximate neutral condition, was placed on the hydrophobic surface of dimethyl dichlorosilane pretreated glass slides, and it was evaporated, the supramolecular complex exhibited the periphery of the droplet due to the capillary effect, and accumulated there to form a red capillary flow directed assembly ring(CFDAR). A typical CFDAR has an outer diameter of (2 r ) about 1.18 mm and a ring width(2 δ ) of about 41 μm. Depending on the experimental conditions, a variety of CFDAR can be assembled. The experimental results are in agreement with our former theoretical discussion. It was found that when a droplet volume is 0.1 μL, the fluorescence intensity of the CFDAR formed by the NR NA is in proportion to the content of calf thymus DNA in the range of 0-0.28 ng, fish sperm DNA of 0-0.24 ng and yeast RNA of 0-0.16 ng with the limit of detection(3 σ ) of 1 7, 1.4 and 0.9 pg, respectively for the three nucleic acids.展开更多
The crystal structure of the title complex salt has been determined by single-crystal X-ray structure analysis. The crystal data are as follows; Monoclinic, P21/c, a=15.6480(10)A,b=16.7870(10)A, c=10.347(2)A, β=90.79...The crystal structure of the title complex salt has been determined by single-crystal X-ray structure analysis. The crystal data are as follows; Monoclinic, P21/c, a=15.6480(10)A,b=16.7870(10)A, c=10.347(2)A, β=90.790(10), V=2717.7(6)A3, Z=3, and R=0.0333 for 4789 unique reflections. The complex anion has a pseudo-octahedral structure distorted more than the CrⅢand CoⅢ analogs, in which cach iminodiacetato ligand (ida2-) is coordinated in a facial fashion with the two N atoms in a cis configuration, resulting in an unsyin-fac structure.展开更多
以盆栽的C4植物-湖南稷子 Echinochloafrumentacea 为材料,用6-苄氨基嘌呤 BA 和脱落酸 ABA 定位涂抹湖南稷子的穗、上位和下位叶片,分析了植物体激素平衡的局部改变对整株水平上Na+、K+和游离脯氨酸分配的调节.实验结果表明,ABA和具有...以盆栽的C4植物-湖南稷子 Echinochloafrumentacea 为材料,用6-苄氨基嘌呤 BA 和脱落酸 ABA 定位涂抹湖南稷子的穗、上位和下位叶片,分析了植物体激素平衡的局部改变对整株水平上Na+、K+和游离脯氨酸分配的调节.实验结果表明,ABA和具有细胞分裂素活性的BA是调控Na+、K+及游离脯氨酸在不同层位叶中分配的重要因素.ABA涂抹湖南稷子的上位叶片,上位叶片中的Na+比其下位叶片高35.0%;用ABA涂抹湖南稷子的下位叶片,下位叶片中的K+比其上位叶片高31.4%,下位叶鞘中的K+比其上位叶鞘高53.7%.用BA涂抹湖南稷子的下位叶片,下位叶片中的K+和脯氨酸分别比其上位叶片高16.5%和31.7%;用BA或ABA定位涂抹植物地上不同部位,引起植物整株水平上Na+、K+向光合作用强的部位,特别是向活跃期的穗中选择性运输的能力增强,游离脯氨酸也多集中于代谢旺盛的光合器官和生殖器官.展开更多
基金Supported by the NationalNaturalScience Foundation of China( No. 2 0 175 0 1) and U niversity Key Teachers Programdirected under the Ministry of Education ofP.R.China( No. 2 0 0 0 - 6 5 )
文摘It is critical to establish a direct and precise method with a high sensitivity and selectivity in analytical chemistry. In this research, making use of a well known phenomenon of capillary flow, we have proposed an image analysis method of nucleic acids at the price of a small amount of sample. When a droplet of the supramolecular complex solution, formed by neutral red and nucleic acids(NA) under an approximate neutral condition, was placed on the hydrophobic surface of dimethyl dichlorosilane pretreated glass slides, and it was evaporated, the supramolecular complex exhibited the periphery of the droplet due to the capillary effect, and accumulated there to form a red capillary flow directed assembly ring(CFDAR). A typical CFDAR has an outer diameter of (2 r ) about 1.18 mm and a ring width(2 δ ) of about 41 μm. Depending on the experimental conditions, a variety of CFDAR can be assembled. The experimental results are in agreement with our former theoretical discussion. It was found that when a droplet volume is 0.1 μL, the fluorescence intensity of the CFDAR formed by the NR NA is in proportion to the content of calf thymus DNA in the range of 0-0.28 ng, fish sperm DNA of 0-0.24 ng and yeast RNA of 0-0.16 ng with the limit of detection(3 σ ) of 1 7, 1.4 and 0.9 pg, respectively for the three nucleic acids.
文摘The crystal structure of the title complex salt has been determined by single-crystal X-ray structure analysis. The crystal data are as follows; Monoclinic, P21/c, a=15.6480(10)A,b=16.7870(10)A, c=10.347(2)A, β=90.790(10), V=2717.7(6)A3, Z=3, and R=0.0333 for 4789 unique reflections. The complex anion has a pseudo-octahedral structure distorted more than the CrⅢand CoⅢ analogs, in which cach iminodiacetato ligand (ida2-) is coordinated in a facial fashion with the two N atoms in a cis configuration, resulting in an unsyin-fac structure.
文摘以盆栽的C4植物-湖南稷子 Echinochloafrumentacea 为材料,用6-苄氨基嘌呤 BA 和脱落酸 ABA 定位涂抹湖南稷子的穗、上位和下位叶片,分析了植物体激素平衡的局部改变对整株水平上Na+、K+和游离脯氨酸分配的调节.实验结果表明,ABA和具有细胞分裂素活性的BA是调控Na+、K+及游离脯氨酸在不同层位叶中分配的重要因素.ABA涂抹湖南稷子的上位叶片,上位叶片中的Na+比其下位叶片高35.0%;用ABA涂抹湖南稷子的下位叶片,下位叶片中的K+比其上位叶片高31.4%,下位叶鞘中的K+比其上位叶鞘高53.7%.用BA涂抹湖南稷子的下位叶片,下位叶片中的K+和脯氨酸分别比其上位叶片高16.5%和31.7%;用BA或ABA定位涂抹植物地上不同部位,引起植物整株水平上Na+、K+向光合作用强的部位,特别是向活跃期的穗中选择性运输的能力增强,游离脯氨酸也多集中于代谢旺盛的光合器官和生殖器官.
