MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attra...MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attracted significant interest, as abnormal miRNA expression is identified to contribute to serious human diseases such as cancers. Traditional methods for miRNA detection do not meet current demands. In particular, nanomaterial-based methods, nucleic acid amplification-based methods such as rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), strand-displacement amplification (SDA) and some enzyme-free amplifications have been employed widely for the highly sensitive detection of miRNA. MiRNA functional research and clinical diagnostics have been accelerated by these new techniques. Herein, we summarize and discuss the recent progress in the development of miRNA detection methods and new applications. This review will provide guidelines for the development of follow-up miRNA detection methods with high sensitivity and specificity, and applicability to disease diagnosis and therapy.展开更多
目的难治性肺炎支原体肺炎及重症肺炎病情重,并发症多,严重影响患儿健康及日后生活,需尽早明确诊断及治疗。本研究采用实时荧光核酸恒温扩增检测技术(simultaneous amplification and testing,SAT)对社区获得性肺炎患儿咽拭子及肺炎支...目的难治性肺炎支原体肺炎及重症肺炎病情重,并发症多,严重影响患儿健康及日后生活,需尽早明确诊断及治疗。本研究采用实时荧光核酸恒温扩增检测技术(simultaneous amplification and testing,SAT)对社区获得性肺炎患儿咽拭子及肺炎支原体肺炎患儿肺泡灌洗液标本进行检测,评估其在儿童肺炎支原体肺炎中应用价值。方法选取2017-09-01-2018-12-31郑州大学附属儿童医院住院的社区获得性肺炎患儿300例,并采用颗粒凝集法检测肺炎支原体血清抗体(pneumonia mycoplasma serum antibody,MP-Ab)、SAT检测肺炎支原体RNA;评估SAT在肺炎支原体肺炎临床诊断中的实用价值。结果SAT检测阳性共128例,其中肺炎支原体肺炎患儿120例,非肺炎支原体肺炎患儿8例;单份肺炎支原体血清抗体阳性125例,其中肺炎支原体肺炎91例,非肺炎支原体肺炎34例。SAT用于诊断肺炎支原体肺炎的真实性(约登指数=0.83)高于单份肺炎支原体血清抗体(约登指数=0.46);SAT用于诊断肺炎支原体肺炎时,与临床的一致性(Kappa值=0.84,P<0.001)高于单份肺炎支原体血清抗体(Kappa值=0.47,P<0.001)。56例肺炎支原体肺炎患儿进行肺泡灌洗检测,灌洗液阳性53例,咽拭子阳性51例,两种标本阳性检出率差异无统计学意义,χ2=2.322,P=0.128。结论SAT在肺炎支原体肺炎的诊断中应用价值高;SAT在不同类型标本中的肺炎支原体检出一致性好。展开更多
基金financial support from the National Natural Science Foundation of China(Grant 81573389)the National Key R&D Program of China(2017YFC0908600)
文摘MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attracted significant interest, as abnormal miRNA expression is identified to contribute to serious human diseases such as cancers. Traditional methods for miRNA detection do not meet current demands. In particular, nanomaterial-based methods, nucleic acid amplification-based methods such as rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), strand-displacement amplification (SDA) and some enzyme-free amplifications have been employed widely for the highly sensitive detection of miRNA. MiRNA functional research and clinical diagnostics have been accelerated by these new techniques. Herein, we summarize and discuss the recent progress in the development of miRNA detection methods and new applications. This review will provide guidelines for the development of follow-up miRNA detection methods with high sensitivity and specificity, and applicability to disease diagnosis and therapy.
