Evaluation of the Efficacy of Alanyl-Glutamine for Nutritional Support in Septicemia

Objective:To evaluate the therapeutic efficacy of alanyl-glutamine(Ala-Gln)in the nutritional support of sepsis.Methods:120 cases of sepsis patients admitted to the hospital in the past three years were selected and grouped by randomization method,Group A was treated with Ala-Gln,and Group B was treated with conventional nutritional support therapy,and the therapeutic effects were compared.Results:Before treatment,there was no difference in nutritional indexes,immune function indexes,and inflammatory factors between the two groups(P>0.05).After treatment,the nutritional indexes of Group A were higher than those of Group B,the immune function indexes were higher than those of Group B,and the inflammatory factors were lower than those of Group B(P<0.05).Except for mortality,the regression of group A was better than that of group B(P<0.05).Conclusion:Ala-Gln can improve the nutritional indexes of septicemia patients during the treatment period,enhance their immune function,reduce the inflammatory response of the body,and promote the regression of the disease.

Prognostic impact of inflammatory and nutritional biomarkers in pancreatic cancer

Pancreatic cancer is usually associated with a poor prognosis.Surgery is the main curative treatment but pancreatic operations are aggressive and new tools that help clinicians to predict surgical and prognostic outcomes are necessary.Lu et al recently published a retrospective,single centre cohort study evaluating the impact of seven nutritional and inflammatory markers in pancreatic cancer surgical patients:The albumin-to-globulin ratio,prognostic nutritional index(PNI),systemic immune-inflammation index(SII),neutrophil-to-lymphocyte ratio(NLR),platelet-to-lymphocyte ratio(PLR),nutritional risk index,and the geriatric nutritional risk index.A significant correlation was found between the PNI,SII,NLR,and PLR and a hospital discharge of less than 15 days.In a univariable analysis,PNI,SII,NLR and PLR were significantly related to recurrence-free survival and,in a multivariable analysis PNI was associated with overall survival.Various meta-analyses corroborate the results in terms of prognosis but individual studies are discordant on their usefulness.Besides,the cut-off values for these markers vary significantly between studies and there are no clinical trials comparing them to identify the most relevant ones.These are limitations when implementing nutritional and inflammatory biomarkers into clinical practice and further studies are needed in order to answer these questions.

Improving postoperative outcomes in patients with pancreatic cancer:Inflammatory and nutritional biomarkers

This editorial assesses the prognostic value of preoperative inflammatory and nutritional biomarkers in patients undergoing surgical resection for pancreatic cancer.Lu et al evaluated the ability of seven biomarkers to predict postoperative recovery and long-term outcomes.These biomarkers were albumin-to-globulin ratio,prognostic nutritional index(PNI),systemic immune-inflammation index,neutrophil-to-lymphocyte ratio,platelet-to-lymphocyte ratio,nutritional risk index,and geriatric nutritional risk index.The PNI was found to be a strong predictor of both overall and recurrence-free survival,underscoring its clinical relevance in managing patients with pancreatic cancer.

Investigation of Dominant Populations of Late-summer Phytoplankton and Comprehensive Nutritional Evaluation of Water Quality in Bailang Lake

[Objective] This study aimed to investigate the dominant populations of late-summer phytoplankton and conduct comprehensive nutritional evaluation of water quality in Bailang Lake. [Method] A series of water quality survey and phytoplankton sampling was conducted in Bailang Lake in September 2011, a total of four sam- pling points were set, to detect and analyze the total nitrogen, total phosphorus, ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and permanganate index in water samples and further conduct comprehensive nutritional evaluation of the water quality factors using comprehensive nutritional state index method. [Result] Water in Bailang Lake was neutral to slightly alkaline with pH of 8.57. The average water trans- parency was 0.4 m, with relatively high content of dissolved oxygen. The content of total nitrogen was relatively high of 3.043 mg/L averagely. Comprehensive nutritional evaluation showed that the water quality of Bailang Lake belonged to moderate eu- trophication. As can be seen from various comprehensive nutritional state indices TLI, the contribution of transparency, chlorophyll a and total nitrogen to the eutrophi- cation in Bailang Lake was the greatest, followed by the permanganate index and total phosphorus. Statistical analysis showed that the average weight-biomass of phy- toplankton in Bailang Lake was 6.442 mg/L, and the average was 146.46x104 individuals/ml. There were eight dominant species in Bailang Lake, including Scenedesmus, Tetraedron, Merismopedia sinica, Phormidium, Merismopedia, Tribonema, Chroomonas and Synedra. [Conclusion] This study provided data informa- tion for the environmental protection and fisheries production in Bailang Lake.

Analysis and Evaluation of Nutritional Quality in Chinese Radish (Raphanus sativus L.)

Radish （Raphanus sativus L.） is an important vegetable crop worldwide. High nutritional quality was critical in its genetic improvement and production. The nutritional quality of 42 Chinese radish cultivars was analyzed in this study. The contents of six nutritional facts, dry matter （DM）, crude fiber （CF）, total soluble sugar （TSS）, vitamin C （Vc）, protein, and nitrate, ranged from 29.7 to 88.2, 4.507 to 18.546, 2.233 to 15.457, 0.1416 to 0.3341, 0.34 to 1.15, and 1.81 to 5.89 g·kg^-1 fresh weight （FW）, respectively. Significant differences among the 42 radish cultivars were detected in the contents of nutritional facts. The data were subjected to cross-correlation analysis and principal component analysis （PCA）. It was found that DM content was positively correlated with the content of TSS （r=0.7104）, Vc （r=0.4011） and protein （r=0.4120）. Vitamin C （Vc） content of radish showed a positive correlation （r = 0.3300） with the protein content. According to the principal component analysis, out of the 42 radish cultivars, Nau-17, Nau-28, Nau-6, Nau-11, Nau-10, Nau-27, and Nau-31 were detected with very high scores in comprehensive evaluation. It could be concluded that abundant diversity of nutritional fact content occurred in different radish genotypes, and PCA analysis was effective for selecting radish germplasm with high quality. The results could contribute useful knowledge of nutritional quality, and provide important germplasms for the elite cultivar development and the inheritance study of nutritional facts in radish.

Sensory Evaluation and Nutritional Composition of Developed Papaya-Gooseberry Jam

Jams are delicious and nutritious spreads typically made from fruit, sugar and pectin that ensure availability of fruits in off-season. The study aimed to develop papaya-gooseberry jam and to evaluate sensory and nutritional parameters. Six variations of papaya: gooseberry jam was prepared besides the control T0 (100% papaya). The successive variations were T1 (95:5), T2 (90:10), T3 (85:15), T4 (80:20), T5 (75:25) and T6 (70:30). T-test and one-way analysis of variance (ANOVA) were applied to find the significant difference in sensory and nutritional parameters in different variations. Among different variations, the treatment (T4) was found to be the best on the basis of sensory evaluation with incorporation levels of 80 percent papaya and 20 percent gooseberry. The identified treatment (T4) was analyzed for nutritional composition in comparison to control (T0). Nutritional analysis showed that treatment (T0) contained maximum energy (353 Kcal/100g), protein (0.50 g/100g), carbohydrate (87.14 g/100g), fat (0.19 g/100g) and beta-carotene (195.0 g/100g) whereas the treatment (T4) was found to be high in nutrients such as fiber (1.32 g/100g), calcium (92.0 mg/100g), phosphorus (14.13 mg/100g), vitamin C (19.16 mg/100g) and antioxidant activity (98.0%). Thus, results signified that treatment (T4) was considered best in sensory and nutritional qualities and gooseberry could be successfully incorporated in fruit jams to enhance the nutritional properties of the food products.

Comprehensive evaluation of nutritional status before and after hematopoietic stem cell transplantation in 170 patients with hematological diseases

Objective： To investigate the nutritional status of patients before and after hematopoietic stem cell transplantation（HSCT）, and explore optimal methods for assessing nutritional status in patients with hematological diseases.Methods： This cohort study enrolled 170 patients who were diagnosed with hematological diseases and underwent allogeneic HSCT in the Department of Hematology, Peking University People＇s Hospital between May2011 and April 2013. We used fixed-point continuous sampling and four nutritional screening tools, Nutritional Risk Screening 2002（NRS-2002）, Mini Nutritional Assessment（MNA）, Subjective Global Assessment（SGA） and Malnutrition Universal Screening Tools（MUST）, in combination with body measurements, to extensively screen and evaluate nutritional risks and status in patients receiving HSCT before entering and after leaving laminar air flow rooms.Results： After HSCT, patients had significant reduction in weight, hip circumference, waist-hip ratio, calf circumference, mid-upper arm circumference, and suprailiac skinfold thickness compared with pre-HSCT measurements. Before HSCT, NRS-2002 identified that 21.2% of patients were at nutritional risks, compared with100% after HSCT. MUST indicated that before HSCT, 11.77% of patients were at high nutritional risk,compared with 59.63% after HSCT. MNA assessed that 0.06% of patients were malnourished before HSCT,compared with 19.27% after HSCT. SGA identified that before HSCT, 1.76% of patients had mild to severe malnutrition, which increased to 83.3% after HSCT. There is a significant increase in the nutritional risk and malnutrition in patients who received HSCT.Conclusions： Before HSCT, some patients already had nutritional risk or nutritional deficiencies, and prompt and close nutritional screening or assessment should be performed. The nutritional status of patients after HSCT was generally deteriorated compared with that before transplantation. Body measurements should be taken more frequently during the subsequent treatment window in the laminar air flow rooms. After HSCT, it is recommended to combine MNA and SGA to fully evaluate the nutritional status, and thus provide timely and reasonable nutritional support.

Nutritional evaluation of different cultivars of potatoes(Solanum tuberosum L.) from China by grey relational analysis(GRA) and its application in potato steamed bread making

Chemical composition(moisture,protein,starch,ash,fiber,fat),vitamins(vitamin C,vitamin B1,vitamin B2),total polyphenol content,antioxidant capacity,minerals,and amino acid of 14 potato cultivars in China were evaluated.The results indicated that all parameters varied significantly among different potato cultivars.The total starch,crude protein and fat content ranged between 57.42–67.83%,10.88–14.10% and 0.10–0.73% dry weight(DW),respectively.Moreover,the consumption of potato increased remarkably the dietary intake of vitamins,K,Mn and Cu.In addition,the chemical score of amino acid varied considerably between different cultivars,which ranged from 54(Neida 3 and Neida 41) to 71(Neida 34).Grey relational analysis(GRA) indicated that Neida 26 exhibited the most comprehensive nutritional values among potato cultivars,followed by Neida 42.Different potato flours had a significant effect on the quality parameters of potato steamed bread(PSB),Neida 26 and Neida 34 were more suitable for making PSB.

Nutritional Evaluation of Complementary Food Formulations from Maize, Soybean and Peanut Fortified with <i>Moringa oleifera</i>Leaf Powder

Nutritional evaluation of complementary food formulations from maize, soybean and peanut fortified with Moringa oleifera leaf powder was carried out. Maize, soybean and peanut were blended in a ratio of 60:30:10 to produce a complementary food, which was then fortified. While the unfortified food product (sample A) served as control, the other three formulations were fortified with 5%, 10% and 15% Moringa leaf powder to give three samples (B, C and D respectively) of fortified food. Nutritional composition determination and feeding trials were then carried out, using two weeks old male albino rats to determine the performance of the food formulations. While the crude protein, crude fibre, and ash contents of the diets increased significantly (p with fortification, with values ranging from 16.04% to 17.59%, 2.25% to 4.42% and 1.40% to 2.50% respectively, crude fat and carbohydrate decreased significantly (p < 0.05), with concomitant decrease in energy, with values ranging from 23.48% to 20.80%, 49.32% to 47.63% and 472.76% to 448.08 kcal/100g respectively in samples A to D. PER values significantly (p < 0.05) improved up to 10% substitution, from 1.77 in unfortified (sample A) to 1.90 in 10% fortified (sample C), but declined at 15% substitution (sample D) to 1.69. Similarly, NPR values increased from 0.71 to 0.76 and 0.68. However, all the PER values including that of Nestle Cerelac (2.04) were lower than, though within the same range, with the value of 2.10 recommended by the Protein Advisory Group (PAG) for complementary foods. Sample C (10% Moringa flour blend) gave the best performance after rat feeding trials.

Nutritional evaluation,fatty acid analysis and anticancer properties investigation of seeds from kan-zaw,a potential diet oil and medicinal plant

Payenapara lleloneura Kurz.(Kan-zaw),an endemic medicinal plant only found in Tanintharyi Region of Myanmar,is widely used in the treatment of various cancer and different ailments.In the present research,the seeds were phytochemical investigated for their nutritional potential for their use as functional foods or novel diet oil resources.Nutritional evaluation showed that the seeds are rich in fats and carbohydrates(soluble sugars and starch).Fatty acid analyses showed that the seeds accumulate very richα-eleostearic acid(α-ESA,18:3^(Δ9 cis,11-trans),13 trans),an important conjugated fatty acid,up to more than 70% of total fatty acids.The seed oil derived from the Kan-zaw tree contains approximately 3.25% β-eleostearic acid(18:3^(Δ9 trans,11 trans,13 trans)),an unusual conjugated fatty acid that imparts a potent anticancer application and industrially important drying qualities to Kan-zaw oil.Physicochemical properties of the Kan-zaw seeds were examined;petroleum ether(60–90℃)extract of seed oils were also investigated for the saponification value,iodine value and estimation of acid value.Further,the present study investigated cytotoxic potential of ethanol,methanol,acetone,chloroform Kan-zaw seed extracts and commercial Kan-zaw oil against human cervical cancer cell line(HeLa).The Kan-zaw extracts and oil have shown significant anticancer activity on HeLa cells.

Nutritional evaluation in cirrhosis: Emphasis on the phase angle

Protein-calorie malnutrition (PCM) is a common condition in cirrhotic patients, leading to a worse prognosis, complications, poor quality of life and lower survival rates. Among ways of assessing nutritional status, there are anthropometric methods such as the evaluation of the triceps skinfold, the arm circumference, the arm muscle circumference and the body mass index, and non-anthropometric methods such as the subjective global assessment, the handgrip strength of non-dominant hand, and the bioelectrical impedance analysis (BIA). PCM is frequently under-diagnosed in clinical settings in patients with cirrhosis due to the limitations of nutritional evaluation methods in this population. BIA is a useful method, but cannot be indicated in patients with abnormal body composition. In these situations, the phase angle (PA) has been used, and can become an important tool in assessing nutritional status in any situation. The PA is superior to anthropometric methods and might be considered as a nutritional indicator in cirrhosis. The early characterization of the nutritional status in patients with cirrhosis means an early nutritional intervention, with a positive impact on patients&rsquo; overall prognosis. Among the usually accepted methods for nutritional diagnosis, the PA provides information in a quick and objective manner.

Nutritional evaluation of caseins and whey proteins and their hydrolysates from Protamex

Whey protein concentrate (WPC 80) and sodium caseinate were hydrolyzed by Protamex to 5%, 10%, 15%, and 20% degree of hydrolysis (DH). WPC 80, sodium caseinate and their hydrolysates were then analyzed, compared and evaluated for their nutritional qualities. Their chemical composition, protein solubility, amino acid composition, essential amino acid index (EAA index), biological value (BV), nutritional index (NI), chemical score, enzymic protein efficiency ratio (E-PER) and in vitro protein digestibility (IVPD) were determined. The results indicated that the enzymatic hydrolysis of WPC 80 and sodium caseinate by Protamex improved the solubility and IVPD of their hydrolysates. WPC 80, sodium caseinate and their hydrolysates were high-quality proteins and had a surplus of essential amino acids compared with the FAO/WHO/UNU (1985) reference standard. The nutritive value of WPC 80 and its hydrolysates was superior to that of sodium caseinate and its hydrolysates as indicated by some nutritional parameters such as the amino acid composition, chemical score, EAA index and predicted BV. However, the E-PER was lower for the WPC hydrolysates as compared to unhydrolyzed WPC 80 but sodium caseinate and its hydrolysates did not differ significantly. The nutritional qualities of WPC 80, sodium caseinate and their hydrolysates were good and make them appropriate for food formulations or as nutritional supplements.

Determination of Content of Amino Acid in Abalone by Precolumn Derivatization and its Nutritional Value Evaluation

[Objectives] This study was conducted to optimize the determination conditions of amino acids from abalone. [Methods] The sample was treated by acid hydrolysis method and subjected to 2,4-2 nitro fluorobenzene column derivatization. The amino acid content in abalone was determined by HLPC,and the nutritional value of the amino acids was evaluated with egg protein model put forward by Institute of Nutrition and Food Hygiene,Chinese Academy of Preventive Medicine. [Results] Abalone contains full amino acids. According to the FAO/WHO ideal,it is a high-quality protein source and suitable for supplement of protein source for human body. [Conclusions]The experimental method has simple operation and could achieve a good effect with wide linear range and correlation coefficient over 0. 999 8,and the obtained results are satisfactory.

Nutritional evaluation of Kedrostis africana (L.) Cogn:An edible wild plant of South Africa

Objective:To evaluate the nutritional composition and elemental constituents of Kedrostis africana and their safety aspect.Methods:Proximate parameters(moisture,ash,crude fibre,crude fat,proteins,and carbohydrate and energy) were evaluated using ALASA methods,and elemental analysis by ICP-OES technique.Results:The results from nutritional analysis showed that the tuber used for this study had a low content of crude fat and high content of ash,crude protein,crude fibre,carbohydrate and energy having the recommended dietary allowances.The tuber was rich in major minerals Na,K,Ca and Mg,there was sufficient amount of trace elements Fe,Cu,and Zn while the anti-nutrients oxalate,phytate,alkaloids,and saponins were detected in amounts that are not harmful according to Food and Agriculture Organization/World Health Organization.Conclusions:The outcome of this study suggests that this wild plant has very good nutritional potentials to meet the recommended dietary allowance and it could be a cheap source of essential nutrients that may ameliorate most nutritional challenges and can contribute remarkably to the amount of nutrient intake in human and animal diet.

Analysis and Evaluation of the Nutritional Components in Holotrichia parallela Motschulsky

The nutritional ingredients of adult Holotrichia parallela Motschulsky were analyzed comprehensively in this study.It was showed that the content of water in H.parallela was 78.39%,and the content of dry substances was 21.61%;among dry substances,crude protein,crude fat,ash and crude fiber accounted for 70.27%,16.26%,3.61% and 10.36%,respectively.The mineral elements,trace mineral elements and heavy metals were also determined by Microwave Digestion-Inductively Coupled Plasma Mass Spectrometry.The results indicated that the contents of major elements including potassium（K）,magnesium（Mg）,Calcium（Ca）,iron（Fe）were rich,especially the potassium（K）,up to 2 851 mg/kg.In addition,the content of copper was 8.770 167 mg/kg,and the selenium was 0.108 232 mg/kg.It was suggested that H.parallela could be used as good human food and animal feed.

Progresses on Personalized Nutritional Evaluation and Recommendation

Health is maintained by a state of dynamic homeostasis in which nutrient intake and ex- penditure are of good balance. Therefore, it is important to know exactly the nutritional value of food sources, as well as the nutritional requirements of individuals, in order to achieve optimal nutrition. Considering the interaction between diet and individual back- ground, nutritional evaluation and recommendation has become a complicate issue needing further investigations. While traditional nutrition research has made significant progress in population nutrition, modern nutrition research is now becoming possible to focus on personalized nutrition in health promotion, disease prevention, performance improvement, and risk assessment of individual with the development of emerging omics technologies. This review tried to summarize the methods used in nutritional evaluation and recom- mendation as well as their applications. Though personal nutrition evaluation and recommendation are still not well-established, utilization of these advanced technologies may expand our knowledge in bioavailability and bioefficacy of diet ingredients, pathophysiological changes in response to dietary intervention, as well as nutrition-associated disease biomarkers discovery, and thus contributing to personalized nutrition.

Nutritional Evaluation by Nurses and Physical Recovery of Malnourished Patients after Esophagectomy

Following an esophagectomy, nurses’ patient nutrition observations are important for physical recovery and quality of life. The study proposed to identify the factors associated with physical recovery among esophageal cancer patients after surgery based on nurses’ observations and nutritional evaluations. Data were collected from the nursing records of 21 esophagectomy patients. The data included patients’ rate of weight loss, body mass index (BMI), energy intake, and postoperative complications. Patients were divided into two groups according to their weight loss rate: <8% and ≥8%. Patient outcomes were compared between the two groups. There was no difference in average BMI before or after surgery between the two groups. There was no difference in albumin levels during hospitalization;however, after esophagectomy, energy intake at discharge was insufficient in both groups. The ≥8% weight loss may be facilitated, or at least confounded, by the presence of recurrent nerve paralysis and aspiration pneumonia. Thus, the nursing records typically identified the need to check for recurrent laryngeal nerve palsy and aspiration pneumonia in conjunction with nutritional assessment to enhance patients’ physical recovery. When weight loss was significant, nurses provided oral intake support with the NST, and patients maintained oral intake levels similar to the group with weight loss under 8%. In order to better support physical recovery among malnourished, post esophagectomy patients, nurses need to monitor for signs of recurrent nerve palsy and aspiration pneumonia in addition to nutritional intake.

Nutritional Analysis and Physiological Activity Evaluation of Pneumatophorus japonicus Sauce

The nutritional composition of Pneumatophorus japonicus sauce was analyzed and compared with other fish sauces. The sodium chloride content of the sample Pneumatophorus japonicus sauce was greater than 25%. The total soluble nitrogen content was greater than 2.1 g/100mL, and the amino nitrogen content was greater than 1.2 g/100mL. The mineral content was abundant in the Pneumatophorus japonicus sauce, the calcium content and the magnesium content were 391.50±0.03 and 375.00±0.02 mg/L respectively, much higher than those in other samples. The iron content and the zinc content were 22.30±0.04 and 2.80±0.03 mg/L, respectively. The content of free amino acids was 5 552.17 mg/100mL, the content of essential amino acids was 2 666.00 mg/100mL,The taurine content was 113.05 mg/100mL. The antioxidant activity of the fermentation broth was 90.48%, while the TCA soluble peptide content was 29.04 mg/mL. Pneumatophorus japonicus sauce is nutritionally rich and has special physiological activity.

Evaluation of Dietary Diversity, Nutrient Adequacy and Nutritional Status of Pre-school Children in Three Local Government Areas of Ibadan, Nigeria

Malnutrition remains a public health challenge in Nigeria, partly due to poor diversity of diets. Pre-school children are among the vulnerable groups who are mostly affected in communities, as their nutritional status is largely influenced by the quality of diets they consume. Most studies aimed at malnutrition among under-five children have been focused on meeting protein-energy needs without much consideration for micronutrients needs. Dietary diversity has been advocated as a means of meeting nutritional requirements of children. This study was designed to assess the dietary diversity in relation to nutrient adequacy and nutritional status of pre-school children in Ibadan. The descriptive cross-sectional study was carried out among 552 pre-schoolers aged 3-5 years in Egbeda, Ona-Ara and Ido Three Local Government Areas (LGAs) of Ibadan metropolis. A structured questionnaire was used to obtain information on socio-demographic characteristics of mother and child. Nutritional status was determined using anthropometric indices. Food frequency questionnaire of 9 food groups was used to obtain dietary diversity scores (DDS) classified as low (< 4 food groups), average (4 to 6 food groups), and high (≥ 7 food groups);and 24-hour dietary recall was used to obtain nutrient adequacy of diet. Data were analysed using descriptive statistics, Pearson’s correlation coefficient, and level of significance set at p < 0.05. Malnutrition among pre-schoolers was more prevalent among males (22.0%) than females (18.4%). Stunting was the most prevalent form of malnutrition (26.8%). The DDS of pre-schoolers was low (3.2 ± 1.9). The most consumed food group was cereals, roots and tubers (83.9%) while fruit was least consumed (6.5%). Nutrient adequacy ratio for carbohydrate was highest (1.2 ± 0.2) and least in fibre (0.2 ± 0.2), while mean adequacy ratio for food consumed was (0.72 ± 0.13). There was weak relationship between DDS and nutritional status—wasting (r =-0.067), stunting (r =-0.002) and underweight (r =-0.056). Low dietary diversity and malnutrition existed among pre-school children in the three LGAs. Nutrition education and enlightenment programmes are required by the parents/guardians of pre-school children on importance of dietary diversity in these areas.

Nutritional and Safety Evaluation of Local Weight-Gain Formulas in the Kingdom of Saudi Arabia (KSA) Markets

This study was conducted to examine the nutritional content, contamination levels, and biological effects of 3 local prepared formulas. Formula (1) contained mixed nuts;formula (2) contained moghat, honey and royal jelly, while formula (3) contained honey, fenugreek and royal jelly. Groups of albino rats (90 rats) were randomly allocated to diets of control group or 1 - 3 treatment groups. The tested diets contained 15% carbohydrate. The weight and food intake were recorded. Blood tests were conducted to determine total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, triglycerides, glucose, urea, creatinine, white blood cell count (WBC), red blood cell count (RBC), haemoglobin (HB), mean corpuscular volume (MCV), and haematocrit (HCT). Results showed that bacterial contamination was found in formula (1) and formula (3). Fungal contamination was detected in formula (2) and formula (3). Body weight increased significantly in the rats fed treatment formulas compared to the control group. The blood tests indicated significant increases in the levels of glucose, WBC, HCT, total cholesterol, LDL cholesterol, and triglycerides as well as a significant decrease in HDL level in the group (2) was fed formula (1) the control. In conclusion, these findings indicate that these local formulas may not be safe, and further studies are required to understand the consequences of a long-term consumption of these formulas.