Objective:Small molecules in snow chrysanthemum such as flavonoids,phenolic compounds and amino acids have been extensively investigated.No study to date has focused on water-soluble oligosaccharides.The objective of ...Objective:Small molecules in snow chrysanthemum such as flavonoids,phenolic compounds and amino acids have been extensively investigated.No study to date has focused on water-soluble oligosaccharides.The objective of this study is identification and determination of water-soluble oligosaccharides in snow chrysanthemum.Methods:The oligosaccharides in snow chrysanthemum were identified by high performance thin layer chromatography(HPTLC),liquid chromatography-mass spectrometry(LC-MS)combined MS library and methylation analysis for the first time.Subsequently the oligosaccharides were determined by high performance liquid chromatography with a charged aerosol detector(HPLC-CAD).Results:The oligosaccharides in snow chrysanthemum were identified as inulin-type fructooligosaccharides(FOS).The yield of FOS(DP3~DP13)in the first extraction was over 97.6%.The RSDs of repeatability in three sample amount levels(0.08 g,0.1 g,0.12 g)are lower than 4.8%and the RSDs of stability are less than 3.5%.The recoveries of FOS(DP3~13)were ranging from 96.9%to 105.6%.The contents of FOS(DP3~DP13)in flowers of snow chrysanthemum from different regions of China were greatly variant.Conclusion:This is the first time to identify and quantify FOS in snow chrysanthemum which is helpful for its performance in the in the fields of biomedical,agriculture and functional food industry as well as development of the quality control methods.In addition,the identification approach developed in this work can also be used for screening potential natural sources containing FOS.展开更多
文摘利用超高效液相色谱-三重四极杆串联质谱联用法(ultra-high performance liquid chromatography-triple quadrupole mass spectrometry, UPLC-QQQ-MS)比较2种寄主肉苁蓉经过不同干燥处理后的10种苯乙醇苷类成分含量。将肉苁蓉干燥品粉碎,粉末过60目筛,用50%(体积分数)甲醇溶解后超声提取,用Agilent Eclipse Plus C18柱(2.1 mm×150 mm, 1.8μm)分离,以A相(0.1%甲酸水溶液)-B(乙腈)相作为流动相进行梯度洗脱,利用电喷雾离子源,在UPLC-QQQ-MS的动态多反应模式下检测。结果表明,不同干燥处理的寄主肉苁蓉苯乙醇苷类成分含量具有明显差异,冷冻干燥处理所得的肉苁蓉中10种苯乙醇苷类含量最高,其次为80℃烘干样品。虽然冷冻干燥对于肉苁蓉所保留10种苯乙醇苷类成分最高,但基于大规模生产考虑,80℃烘干更具有经济效益。
基金supported by the National Key Research and Development Project(SQ2018YPC170480,2018YFC1708202)the National Natural Science Foundation of China(81774009)+1 种基金the Key science and technology projects of Inner Mongolia Autonomous Region(2019ZD004)Open Project Program of Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology,China(MDK 2019040)。
文摘Objective:Small molecules in snow chrysanthemum such as flavonoids,phenolic compounds and amino acids have been extensively investigated.No study to date has focused on water-soluble oligosaccharides.The objective of this study is identification and determination of water-soluble oligosaccharides in snow chrysanthemum.Methods:The oligosaccharides in snow chrysanthemum were identified by high performance thin layer chromatography(HPTLC),liquid chromatography-mass spectrometry(LC-MS)combined MS library and methylation analysis for the first time.Subsequently the oligosaccharides were determined by high performance liquid chromatography with a charged aerosol detector(HPLC-CAD).Results:The oligosaccharides in snow chrysanthemum were identified as inulin-type fructooligosaccharides(FOS).The yield of FOS(DP3~DP13)in the first extraction was over 97.6%.The RSDs of repeatability in three sample amount levels(0.08 g,0.1 g,0.12 g)are lower than 4.8%and the RSDs of stability are less than 3.5%.The recoveries of FOS(DP3~13)were ranging from 96.9%to 105.6%.The contents of FOS(DP3~DP13)in flowers of snow chrysanthemum from different regions of China were greatly variant.Conclusion:This is the first time to identify and quantify FOS in snow chrysanthemum which is helpful for its performance in the in the fields of biomedical,agriculture and functional food industry as well as development of the quality control methods.In addition,the identification approach developed in this work can also be used for screening potential natural sources containing FOS.
