Objective:To evaluate the phytochemical constituents and the antioxidant activity of hydroalcoholic extract of Nymphaea nouchali seed locally prescribed as a diet for diabetes mellitus.Methods:The antioxidant and free...Objective:To evaluate the phytochemical constituents and the antioxidant activity of hydroalcoholic extract of Nymphaea nouchali seed locally prescribed as a diet for diabetes mellitus.Methods:The antioxidant and free radical scavenging activity of hydroalcoholic extract of the plant was assessed against 1,1 diphenyl-2-picryl hydrazyl(DPPH),nitric oxide and lipid peroxidation using standard protocols.Total phenolics,flavonoids and tannins were also determined.Results:Phytochemical analysis revealed the presence of phenols,flavones,tannins,protein,reducing sugars,glycosides,saponins,alkaloids and steroids.The activities of plant extract against DPPH,nitric oxide and lipid peroxidation was concentration dependent with IG_(50)value of42.82,23.58 and 54.65μg/mL respectively.The total antioxidant capacity was high with 577.73 mg vitamin E/g of the extract and showed a moderately high vitamin C content of 197.22 mg/g.The total tannin content of hydroalcoholic seed extract was high(195.84 GE/g),followed by phenolics(179.56 GE/g)and flavonoids(23.55 QE/g).Conclusion:Our findings provide evidence that the crude extract of Nymphaea nouchali is a potential source of natural antioxidants and this justifies its use in folkloric medicine.展开更多
[ Objective] The study aimed to explore the approaches of utilizing Nymphaea tetragona Georgi by doing research on removing nitrogen and phosphorus from sewage using N. tetragona Georgi. [Method] With N. tetragona Geo...[ Objective] The study aimed to explore the approaches of utilizing Nymphaea tetragona Georgi by doing research on removing nitrogen and phosphorus from sewage using N. tetragona Georgi. [Method] With N. tetragona Georgi as the research object, a comparative analysis on the content of total nitrogen （TN） and total phosphorus （TP） was carded out after N. tetragona Georgi was planted in sewage. TN content was deter- mined by alkaline potassium persulfate digestion -UV spectrophotometric method （GB11894 -89）, and TP content was measured by using ammo- nium molybdate spectrophotometric method （GBl1893 -89）. [ Result] The removal rate of TN and TP reached 12.31% and 8.64% respectively under natural conditions after N. tetragona Georgi was cultivate in the sewage for 13 days. Afterward, the removal rate has no significant change. [ Conclusion] N. tetragona Georgi can purify sewage to some degree, and the removal efficiency of TN was higher than that of TP.展开更多
Studies conducted in the delta and lower valley of Senegal river have demonstrated through ethnobotanical surveys, the importance of Nymphaea lotus L. and Nymphaea micrantha Guill. et Perr in the diet and in the pharm...Studies conducted in the delta and lower valley of Senegal river have demonstrated through ethnobotanical surveys, the importance of Nymphaea lotus L. and Nymphaea micrantha Guill. et Perr in the diet and in the pharmacopoeia. The seeds are used with good appreciation by the indigenous population as a welding food. But studies addressing their biochemical and nutritional aspects are still very rare in the literature. This study was initiated in order to fill this gap and allow a better appreciation of the nutritional and energetic potentialities of these seeds. For this purpose, seeds were collected in effluent from the Senegal River, dried in the shade and put into three different batches: a batch of red seeds of N. lotus, a batch of Black seeds of N. lotus and a batch of seeds of N. micrantha. The biochemical analyses gave their moisture content (9.17% ± 0.2% to 13.36% ± 3.46%), aw (0.46% to 0.71%), protein (7.90% ± 1.25% to 8.24% ± 0.5%), lipid (2.32% ± 0.45% to 4.29% ± 1.0%), carbohydrate (74.49% ± 0.9% and 77.66% ± 1.65%), mineral matter (1.77% ± 0.06% to 2.55% ± 0.08%). All three types of seeds have high levels of potassium (481.10% ± 17.62% to 770.81% ± 42.33%), magnesium (312.71% ± 14.08% to 518.67% ± 15.23%) and calcium (188.81% ± 8.52% to 296.52% ± 16%. Their energy values (350.54 ± 15.21 kcal/100g to 375.7 ± 3.48 Kcal/100g), are closed to other cereals consumed in the human diet in Senegal. Therefore, these seeds offer the opportunity to diversify the diet in the delta and lower valley of the Senegal River.展开更多
Objective:To investigate bioactive phytochemicals and antioxidant activities of Nymphaea nouchali and to explore its anticancer pathways by a network pharmacology approach.Methods:Using a spectrophotometer and high-pe...Objective:To investigate bioactive phytochemicals and antioxidant activities of Nymphaea nouchali and to explore its anticancer pathways by a network pharmacology approach.Methods:Using a spectrophotometer and high-performance liquid chromatography-diode array detector(HPLC-DAD),we quantified bioactive phytochemicals in methanolic extract of Nymphaea nouchali tuber.The extracts were investigated for in vitro antioxidant properties.Targets of these bioactive phytochemicals were predicted and anticancer-associated pathways were analyzed by a network pharmacology approach.Moreover,we identified the predicted genes associated with cancer pathways and the hub genes in the protein-protein interaction network of predicted genes.Results:Quantitative results indicated the total phenolics,total flavonoids,and total proanthocyanidins in the methanolic extract of Nymphaea nouchali tuber.HPLC-DAD analysis showed rutin(39.44 mg),catechin(39.20 mg),myricetin(30.77 mg),ellagic acid(11.05 mg),gallic acid(3.67 mg),vanillic acid(0.75 mg),rosmarinic acid(4.81 mg),p-coumaric acid(3.35 mg),and quercetin(0.90 mg)in 1 g of dry extract.The extract showed the radical scavenging activities of 2,2-diphenyl-1-picrylhydrazyl,2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)and N,N-dimethyl-pphenylenediamine.By using network pharmacology,we predicted 130 target genes associated with cancer pathways.The top hub genes(IL6,AKT1,EGFR,JUN,PTGS2,MAPK3,CASP3,and CXCL8)were also identified,which were associated with cancer pathways and interacted with bioactive phytochemicals of the methanolic extract of Nymphaea nouchali tuber.Conclusions:Our study provides insights into the mechanism of anticancer activities of the methanolic extract of Nymphaea nouchali tuber.展开更多
基金Supported by the University Grants Commission,Maulana Azad National Fellowship Scheme 2012-2013,Government of India.Grant No.MANF-201213-CHR-TAM-15932
文摘Objective:To evaluate the phytochemical constituents and the antioxidant activity of hydroalcoholic extract of Nymphaea nouchali seed locally prescribed as a diet for diabetes mellitus.Methods:The antioxidant and free radical scavenging activity of hydroalcoholic extract of the plant was assessed against 1,1 diphenyl-2-picryl hydrazyl(DPPH),nitric oxide and lipid peroxidation using standard protocols.Total phenolics,flavonoids and tannins were also determined.Results:Phytochemical analysis revealed the presence of phenols,flavones,tannins,protein,reducing sugars,glycosides,saponins,alkaloids and steroids.The activities of plant extract against DPPH,nitric oxide and lipid peroxidation was concentration dependent with IG_(50)value of42.82,23.58 and 54.65μg/mL respectively.The total antioxidant capacity was high with 577.73 mg vitamin E/g of the extract and showed a moderately high vitamin C content of 197.22 mg/g.The total tannin content of hydroalcoholic seed extract was high(195.84 GE/g),followed by phenolics(179.56 GE/g)and flavonoids(23.55 QE/g).Conclusion:Our findings provide evidence that the crude extract of Nymphaea nouchali is a potential source of natural antioxidants and this justifies its use in folkloric medicine.
文摘[ Objective] The study aimed to explore the approaches of utilizing Nymphaea tetragona Georgi by doing research on removing nitrogen and phosphorus from sewage using N. tetragona Georgi. [Method] With N. tetragona Georgi as the research object, a comparative analysis on the content of total nitrogen （TN） and total phosphorus （TP） was carded out after N. tetragona Georgi was planted in sewage. TN content was deter- mined by alkaline potassium persulfate digestion -UV spectrophotometric method （GB11894 -89）, and TP content was measured by using ammo- nium molybdate spectrophotometric method （GBl1893 -89）. [ Result] The removal rate of TN and TP reached 12.31% and 8.64% respectively under natural conditions after N. tetragona Georgi was cultivate in the sewage for 13 days. Afterward, the removal rate has no significant change. [ Conclusion] N. tetragona Georgi can purify sewage to some degree, and the removal efficiency of TN was higher than that of TP.
文摘Studies conducted in the delta and lower valley of Senegal river have demonstrated through ethnobotanical surveys, the importance of Nymphaea lotus L. and Nymphaea micrantha Guill. et Perr in the diet and in the pharmacopoeia. The seeds are used with good appreciation by the indigenous population as a welding food. But studies addressing their biochemical and nutritional aspects are still very rare in the literature. This study was initiated in order to fill this gap and allow a better appreciation of the nutritional and energetic potentialities of these seeds. For this purpose, seeds were collected in effluent from the Senegal River, dried in the shade and put into three different batches: a batch of red seeds of N. lotus, a batch of Black seeds of N. lotus and a batch of seeds of N. micrantha. The biochemical analyses gave their moisture content (9.17% ± 0.2% to 13.36% ± 3.46%), aw (0.46% to 0.71%), protein (7.90% ± 1.25% to 8.24% ± 0.5%), lipid (2.32% ± 0.45% to 4.29% ± 1.0%), carbohydrate (74.49% ± 0.9% and 77.66% ± 1.65%), mineral matter (1.77% ± 0.06% to 2.55% ± 0.08%). All three types of seeds have high levels of potassium (481.10% ± 17.62% to 770.81% ± 42.33%), magnesium (312.71% ± 14.08% to 518.67% ± 15.23%) and calcium (188.81% ± 8.52% to 296.52% ± 16%. Their energy values (350.54 ± 15.21 kcal/100g to 375.7 ± 3.48 Kcal/100g), are closed to other cereals consumed in the human diet in Senegal. Therefore, these seeds offer the opportunity to diversify the diet in the delta and lower valley of the Senegal River.
基金carried out under the research and development(R and D)project of the Bangladesh Council of Scientific and Industrial Research
文摘Objective:To investigate bioactive phytochemicals and antioxidant activities of Nymphaea nouchali and to explore its anticancer pathways by a network pharmacology approach.Methods:Using a spectrophotometer and high-performance liquid chromatography-diode array detector(HPLC-DAD),we quantified bioactive phytochemicals in methanolic extract of Nymphaea nouchali tuber.The extracts were investigated for in vitro antioxidant properties.Targets of these bioactive phytochemicals were predicted and anticancer-associated pathways were analyzed by a network pharmacology approach.Moreover,we identified the predicted genes associated with cancer pathways and the hub genes in the protein-protein interaction network of predicted genes.Results:Quantitative results indicated the total phenolics,total flavonoids,and total proanthocyanidins in the methanolic extract of Nymphaea nouchali tuber.HPLC-DAD analysis showed rutin(39.44 mg),catechin(39.20 mg),myricetin(30.77 mg),ellagic acid(11.05 mg),gallic acid(3.67 mg),vanillic acid(0.75 mg),rosmarinic acid(4.81 mg),p-coumaric acid(3.35 mg),and quercetin(0.90 mg)in 1 g of dry extract.The extract showed the radical scavenging activities of 2,2-diphenyl-1-picrylhydrazyl,2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)and N,N-dimethyl-pphenylenediamine.By using network pharmacology,we predicted 130 target genes associated with cancer pathways.The top hub genes(IL6,AKT1,EGFR,JUN,PTGS2,MAPK3,CASP3,and CXCL8)were also identified,which were associated with cancer pathways and interacted with bioactive phytochemicals of the methanolic extract of Nymphaea nouchali tuber.Conclusions:Our study provides insights into the mechanism of anticancer activities of the methanolic extract of Nymphaea nouchali tuber.
