AIM:To construct the recombinant lentivirus expression plasmid,pLenti6/V5-NT4 p53(N15)-antennapedia(Ant),and study its effect on HepG2 cells.METHODS:Plasmid pLenti6/V5-NT4 p53(N15)-Ant was constructed incorporating th...AIM:To construct the recombinant lentivirus expression plasmid,pLenti6/V5-NT4 p53(N15)-antennapedia(Ant),and study its effect on HepG2 cells.METHODS:Plasmid pLenti6/V5-NT4 p53(N15)-Ant was constructed incorporating the following functional regions,including signal peptide sequence and pro-region of neurotrophin 4,N-terminal residues 12-26 of p53 and 17 amino acid drosophila carrier protein,Ant.Hepatocellular carcinoma(HepG2)cells were used for transfection.3-[4,5-dimethyl-thiazol-2yl]-2,5 diphenyl tetrazolium bromide(MTT)assay,lactate dehydrogenase(LDH)release assay,transmission electron microscopy(TEM)and flow cytometric analysis(FCM)were employed to investigate the effects of LV-NT4(Si)-p53(N15)-Ant in vitro on HepG2 cells.In vivo experiment was also performed to investigate the inhibitory effect of LV-NT4(Si)-p53(N15)-Ant on tumor growth in nude mice.RESULTS:LV-NT4(Si)-p53(N15)-Ant significantly suppressed the growth of HepG2 cells.MTT assay showed that the growth of HepG2 cells was mucj more significantly inhibited by LV-NT4(Si)-p53(N15)-Ant than by LV-EGFP.The inhibition rate for HepG2 cell growth in the two groups was 46.9% and 94.5%,respectively,48 h after infection with LV-NT4(Si)-p53(N15)-Ant,and was 33.9% and 95.8%,respectively,72 h after infection with LV-NT4(Si)-p53(N15)-Ant(P < 0.01).Light microscopy and TEM showed morphological changes in HepG2 cells infected with LV-NT4(Si)-p53(N15)-Ant,but no signif icant changes in HepG2 cells infected with LV-EGFP.Changes were observed in ultra-structure of HepG2 cells infected with LV-NT4(Si)-p53(N15)-Ant,with degraded membranes,resulting in necrosis.LDH release from HepG2 cells was analyzed at 24,48,72 and 96 h after infection with LV-NT4(Si)-p53(N15)-Ant and LV-EGFP,which showed that LDH release was signif icantly higher in LV-NT4(Si)-p53(N15)-Ant treatment group(682 IU/L)than in control group(45 IU/L,P < 0.01).The longer the time was after infection,the bigger the difference was in LDH release.FCM analysis showed that LV-NT4(Si)-p53(N15)-Ant could induce two different kinds of cell death:necrosis and apoptosis,with apoptosis being the minor type and necrosis being the main type,suggesting that LV-NT4(Si)-p53(N15)-Ant exerts its anticancer effect on HepG2 cells by inducing necrosis.The in vivo study showed that LV-NT4(Si)-p53(N15)-Ant significantly inhibited tumor growth with an inhibition rate of 66.14% in terms of tumor size and weight.展开更多
[Na (N- (p-chlorophenyl ) aza-15--crown--5 ) (Et2O )]2 (Na2W0.5 Mo7.5 O26), Mr= 2119. 4, monoclinic, space group P21/c, a= 17. 803(4), b= 13. 674 (3), c= 14. 610(3), β= 112. 33(3)°, V=3290(1) A3, Z= 2, Dc= 2. 13...[Na (N- (p-chlorophenyl ) aza-15--crown--5 ) (Et2O )]2 (Na2W0.5 Mo7.5 O26), Mr= 2119. 4, monoclinic, space group P21/c, a= 17. 803(4), b= 13. 674 (3), c= 14. 610(3), β= 112. 33(3)°, V=3290(1) A3, Z= 2, Dc= 2. 139 g/cm3, μ= 1.704 mm-1, F (000) = 2079, R = 0. 026, Rw= 0. 030. The supermolecular complexconsists of one [Na2W0.5Mo7.5O28)2- anion in which only two Mo atoms are replaced byW in 25% (M= l/4W+3/4Mo) and the two Na+ are linked symmetrically to neighbouring [W0.5Mo7.5O26]4- units on the right and the left respectively and two complexcanons in which each Na+ deviates from the plane of the four ether oxygens and is coordinated to every hetero--atom in the crownether ring, and is also coordinated to the Oatom of Et2O.展开更多
The mean activity coefficient of 5, 10,15 , 20-tetrakis (P-methoxyl-O-sulfophenyl)porphyrin sodium in dilute aqueous solution has been determined in the modality range 0. 00547-0. 08871 mol · kg-1at 273. 2 K by t...The mean activity coefficient of 5, 10,15 , 20-tetrakis (P-methoxyl-O-sulfophenyl)porphyrin sodium in dilute aqueous solution has been determined in the modality range 0. 00547-0. 08871 mol · kg-1at 273. 2 K by the freezing-point depression method . The results of γ± are 0. 9945-0. 7695, it is in close agreement with that by isopiestic method.展开更多
针对硝酸盐对地下水污染的严重性 ,介绍了用 Ca O除去 CO2 和 H2 O的测定氮同位素比值的燃烧管方法和利用 Ag NO3+C(石墨 )生成 CO2 的测定 NO-3 中氧同位素比值的燃烧法 ;研究了用 15N和 18O同位素分析地下水中 NO-3 的来源和判断硝化...针对硝酸盐对地下水污染的严重性 ,介绍了用 Ca O除去 CO2 和 H2 O的测定氮同位素比值的燃烧管方法和利用 Ag NO3+C(石墨 )生成 CO2 的测定 NO-3 中氧同位素比值的燃烧法 ;研究了用 15N和 18O同位素分析地下水中 NO-3 的来源和判断硝化作用和反硝化作用的发生机理。展开更多
基金Supported by The National Natural Science Foundation of China,No.30471942the Key Science Research Project of Shaanxi Province,No.2004k11-G3
文摘AIM:To construct the recombinant lentivirus expression plasmid,pLenti6/V5-NT4 p53(N15)-antennapedia(Ant),and study its effect on HepG2 cells.METHODS:Plasmid pLenti6/V5-NT4 p53(N15)-Ant was constructed incorporating the following functional regions,including signal peptide sequence and pro-region of neurotrophin 4,N-terminal residues 12-26 of p53 and 17 amino acid drosophila carrier protein,Ant.Hepatocellular carcinoma(HepG2)cells were used for transfection.3-[4,5-dimethyl-thiazol-2yl]-2,5 diphenyl tetrazolium bromide(MTT)assay,lactate dehydrogenase(LDH)release assay,transmission electron microscopy(TEM)and flow cytometric analysis(FCM)were employed to investigate the effects of LV-NT4(Si)-p53(N15)-Ant in vitro on HepG2 cells.In vivo experiment was also performed to investigate the inhibitory effect of LV-NT4(Si)-p53(N15)-Ant on tumor growth in nude mice.RESULTS:LV-NT4(Si)-p53(N15)-Ant significantly suppressed the growth of HepG2 cells.MTT assay showed that the growth of HepG2 cells was mucj more significantly inhibited by LV-NT4(Si)-p53(N15)-Ant than by LV-EGFP.The inhibition rate for HepG2 cell growth in the two groups was 46.9% and 94.5%,respectively,48 h after infection with LV-NT4(Si)-p53(N15)-Ant,and was 33.9% and 95.8%,respectively,72 h after infection with LV-NT4(Si)-p53(N15)-Ant(P < 0.01).Light microscopy and TEM showed morphological changes in HepG2 cells infected with LV-NT4(Si)-p53(N15)-Ant,but no signif icant changes in HepG2 cells infected with LV-EGFP.Changes were observed in ultra-structure of HepG2 cells infected with LV-NT4(Si)-p53(N15)-Ant,with degraded membranes,resulting in necrosis.LDH release from HepG2 cells was analyzed at 24,48,72 and 96 h after infection with LV-NT4(Si)-p53(N15)-Ant and LV-EGFP,which showed that LDH release was signif icantly higher in LV-NT4(Si)-p53(N15)-Ant treatment group(682 IU/L)than in control group(45 IU/L,P < 0.01).The longer the time was after infection,the bigger the difference was in LDH release.FCM analysis showed that LV-NT4(Si)-p53(N15)-Ant could induce two different kinds of cell death:necrosis and apoptosis,with apoptosis being the minor type and necrosis being the main type,suggesting that LV-NT4(Si)-p53(N15)-Ant exerts its anticancer effect on HepG2 cells by inducing necrosis.The in vivo study showed that LV-NT4(Si)-p53(N15)-Ant significantly inhibited tumor growth with an inhibition rate of 66.14% in terms of tumor size and weight.
文摘[Na (N- (p-chlorophenyl ) aza-15--crown--5 ) (Et2O )]2 (Na2W0.5 Mo7.5 O26), Mr= 2119. 4, monoclinic, space group P21/c, a= 17. 803(4), b= 13. 674 (3), c= 14. 610(3), β= 112. 33(3)°, V=3290(1) A3, Z= 2, Dc= 2. 139 g/cm3, μ= 1.704 mm-1, F (000) = 2079, R = 0. 026, Rw= 0. 030. The supermolecular complexconsists of one [Na2W0.5Mo7.5O28)2- anion in which only two Mo atoms are replaced byW in 25% (M= l/4W+3/4Mo) and the two Na+ are linked symmetrically to neighbouring [W0.5Mo7.5O26]4- units on the right and the left respectively and two complexcanons in which each Na+ deviates from the plane of the four ether oxygens and is coordinated to every hetero--atom in the crownether ring, and is also coordinated to the Oatom of Et2O.
文摘The mean activity coefficient of 5, 10,15 , 20-tetrakis (P-methoxyl-O-sulfophenyl)porphyrin sodium in dilute aqueous solution has been determined in the modality range 0. 00547-0. 08871 mol · kg-1at 273. 2 K by the freezing-point depression method . The results of γ± are 0. 9945-0. 7695, it is in close agreement with that by isopiestic method.
文摘针对硝酸盐对地下水污染的严重性 ,介绍了用 Ca O除去 CO2 和 H2 O的测定氮同位素比值的燃烧管方法和利用 Ag NO3+C(石墨 )生成 CO2 的测定 NO-3 中氧同位素比值的燃烧法 ;研究了用 15N和 18O同位素分析地下水中 NO-3 的来源和判断硝化作用和反硝化作用的发生机理。
