A variety of luminol derivatives with N-and O-substitution have been synthesized with broad functional group tolerance. O-esterification has been demonstrated for the first time as a promising way to prepare enhanced ...A variety of luminol derivatives with N-and O-substitution have been synthesized with broad functional group tolerance. O-esterification has been demonstrated for the first time as a promising way to prepare enhanced CL reagents for sensing hemin, bloodstain and horseradish peroxidase(HRP). The most effective analogue with a deuterated acetyl group exhibited greater potential than luminol for bloodstain imaging and HRP imaging in western blotting(WB). In addition, O-etherification can greatly suppress CL signal that has been applied to design a CL probe for β-glucosidase(β-Glu). This study offers important and useful information regarding the luminol modification and shows great potential to use O-substituted analogues for enhanced CL analysis.展开更多
基金projects of the Department of Education of Guangdong Province(Nos.2021ZDZX2030 and 2020ZDZX2017)Guangdong Basic and Applied Basic Research Foundation(No.2021A1515110115)Guangzhou Basic and Applied Basic Research Foundation(No.202102020548)for funding support。
文摘A variety of luminol derivatives with N-and O-substitution have been synthesized with broad functional group tolerance. O-esterification has been demonstrated for the first time as a promising way to prepare enhanced CL reagents for sensing hemin, bloodstain and horseradish peroxidase(HRP). The most effective analogue with a deuterated acetyl group exhibited greater potential than luminol for bloodstain imaging and HRP imaging in western blotting(WB). In addition, O-etherification can greatly suppress CL signal that has been applied to design a CL probe for β-glucosidase(β-Glu). This study offers important and useful information regarding the luminol modification and shows great potential to use O-substituted analogues for enhanced CL analysis.
