ROS-hormone interaction in regulating integrative défense signaling of plant cell

The elaborate redox network of the cell,comprising of events like turnover of reactive oxygen species(ROS),redox sensing,signaling,expression of redox-sensitive genes,etc.,often orchestrates with other bonafide hormonal signaling pathways through their synergistic or antagonistic action in the plant cell.The redox cue generated in plant cells under fluctuating environmental conditions can significantly influence other hormonal biosynthetic or signaling mechanisms,thereby modulating physiology towards stress acclimation and defense.There is also strong evidence of the recruitment of ROS as a‘second messenger’in different hormonal signaling pathways under stress.Moreover,the retrograde signaling initiated by ROS also found to strongly influence hormonal homeostasis and signaling.The present review,in this aspect,is an effort towards understanding the regulatory roles of ROS in integrating and orchestrating other hormonal signaling pathways or vice versa so as to unfold the relationship between these two signaling episodes of plant cells under environmental odds.We also accentuate the significance of understanding the utterly complex interactions,which occur both at metabolic and genetic levels between ROS and phytohormones during stress combinations.Furthermore,the significant and decisive role of ROS turnover,particularly the contribution of RBOH(respiratory burst oxidase homologs)in the synergism of redox and hormone signaling during systemic acquired acclimation under stress is also discussed.

Integrated system of traditional THz time-domain spectroscopy and asynchronous optical sampling

Terahertz time-domain spectroscopy(THz-TDS)system,as a new means of spectral analysis and detection,plays an increasingly pivotal role in basic scientific research.However,owing to the long scanning time of the traditional THz-TDS system and the complex control of the asynchronous optical scanning(ASOPS)system,which requires frequent calibration,we combine traditional THz-TDS and ASOPS systems to form a composite system and propose an all-fiber trigger signal generation method based on the time overlapping interference signal generated by the collinear motion of two laser pulses.Finally,the time-domain and frequency-domain spectra are obtained by using two independent systems in the integrated systems.It is found that the full width at half maximum(FWHM)of the time-domain spectra and the spectral width of the frequency-domain spectra are almost the same,but the sampling speed of the ASOPS system is significantly faster than that of the traditional THz-TDS system,which conduces to the study of the transient characteristics of substances.

Study of CMOS integrated signal processing circuit in capacitive sensors

A CMOS integrated signal processing circuit based on capacitance resonance principle whose structure is simple in capacitive sensors is designed. The waveform of output voltage is improved by choosing bootstrap reference current mirror with initiate circuit, CMOS analogy switch and positive feedback of double-stage inverter in the circuit. Output voltage of this circuit is a symmetric square wave signal. The variation of sensitive capacitance, which is part of the capacitive sensors, can be denoted by the change of output voltage’s frequency. The whole circuit is designed with 1.5 μm P-well CMOS process and simulated by PSpice software. Output frequency varies from 261.05 kHz to 47.93 kHz if capacitance varies in the range of 1PF～15PF. And the variation of frequency can be easily detected using counter or SCU.

Quick System-Level DDR3 Signal Integrity Simulation Research

Double data rate synchronous dynamic random access memory （DDR3） has become one of the most mainstream applications in current server and computer systems. In order to quickly set up a system-level signal integrity （SI） simulation flow for the DDR3 interface, two system-level SI simulation methodologies, which are board-level S-parameter extraction in the frequency-domain and system-level simulation assumptions in the time domain, are introduced in this paper. By comparing the flow of Speed2000 and PowerSI/Hspice, PowerSI is chosen for the printed circuit board （PCB） board-level S-parameter extraction, while Tektronix oscilloscope （TDS7404） is used for the DDR3 waveform measurement. The lab measurement shows good agreement between simulation and measurement. The study shows that the combination of PowerSI and Hspice is recommended for quick system-level DDR3 SI simulation.

Novel non-coherent integration method using binary phase-coded radar signal

The m series with 511 bits is taken as an example being applied in non-coherent integra- tion algorithm. A method to choose the bi-phase code is presented, which is 15 kinds of codes are picked out of 511 kinds of m series to do non-coherent integration. It is indicated that the power in- creasing times of larger target sidelobe is less than the power increasing times of smaller target main- lobe because of the larger target＇ s pseudo-randomness. Smaller target is integrated from larger tar- get sidelobe, which strengthens the detection capability of radar for smaller targets. According to the sidelobes distributing characteristic, a method is presented in this paper to remove the estimated sidelobes mean value for signal detection after non-coherent integration. Simulation results present that the SNR of small target can be improved approximately 6. 5 dB by the proposed method.

Reduction of signal reflection along through silicon via channel in high-speed three-dimensional integration circuit

The through silicon via （TSV） technology has proven to be the critical enabler to realize a three-dimensional （3D） gigscale system with higher performance but shorter interconnect length. However, the received digital signal after trans- mission through a TSV channel, composed of redistribution layers （RDLs）, TSVs, and bumps, is degraded at a high data-rate due to the non-idealities of the channel. We propose the Chebyshev multisection transformers to reduce the signal reflec- tion of TSV channel when operating frequency goes up to 20 GHz, by which signal reflection coefficient （$11） and signal transmission coefficient （$21） are improved remarkably by 150% and 73.3%, respectively. Both the time delay and power dissipation are also reduced by 4% and 13.3%, respectively. The resistance-inductance-conductance-capacitance （RLGC） elements of the TSV channel are iterated from scattering （S）-parameters, and the proposed method of weakening the signal reflection is verified using high frequency simulator structure （HFSS） simulation software by Ansoft.

3D printing of osteocytic Dll4 integrated with PCL for cell fate determination towards osteoblasts in vitro

Since 3D printed hard materials could match the shape of bone,cell survival and fate determination towards osteoblasts in such materials have become a popular research target.In this study,a scaffold of hardmaterial for 3D fabrication was designed to regulate developmental signal(Notch)transduction guiding osteoblast differentiation.We established a polycaprolactone(PCL)and cell-integrated 3D printing system(PCI3D)to reciprocally print the beams of PCL and cell-laden hydrogel for a module.This PCI3D module holds good cell viability of over 87%,whereas cells show about sixfold proliferation in a 7-day culture.The osteocytic MLO-Y4 was engineered to overexpress Notch ligand Dll4,making up 25%after mixing with 75%stromal cells in the PCI3D module.Osteocytic Dll4,unlike other delta-like family members such as Dll1 or Dll3,promotes osteoblast differentiation and themineralization of primary mouse and a cell line of bone marrow stromal cells when cultured in a PCI3D module for up to 28 days.Mechanistically,osteocytic Dll4 could not promote osteogenic differentiation of the primary bone marrow stromal cells(BMSCs)after conditional deletion of the Notch transcription factor RBPjκby Cre recombinase.These data indicate that osteocytic Dll4 activates RBPjκ-dependent canonical Notch signaling in BMSCs for their oriented differentiation towards osteoblasts.Additionally,osteocytic Dll4 holds a great potential for angiogenesis in human umbilical vein endothelial cells within modules.Our study reveals that osteocytic Dll4 could be the osteogenic niche determining cell fate towards osteoblasts.This will open a new avenue to overcome the current limitation of poor cell viability and low bioactivity of traditional orthopedic implants.

Long term integration of radar signals with unknown Doppler shift for ubiquitous radar

Ubiquitous radar is a new radar system that provides continuous and uninterrupted multifunction capability within a coverage volume. Continuous coverage from close-in ＂pop-up＂ targets in clutter to long-range targets impacts selection of waveform parameters. The coherent processing interval （CPI） must be long enough to achieve a certain signal-to-noise ratio （SNR） that ensures the efficiency of detection. The condition of detection in the case of low SNR is analyzed, and three different cases that would occur during integration are discussed and a method to determine the CPI is presented. The simulation results show that targets detection with SNR as low as -26 dB in the experimental system can possibly determine the CPI.

Digital synthesis of programmable photonic integrated circuits

Programmable photonic waveguide meshes can be programmed into many different circuit topologies and thereby provide a variety of functions.Due to the complexity of the signal routing in a general mesh,a particular synthesis algorithm often only accounts for a specific function with a specific cell configuration.In this paper,we try to synthesize the programmable waveguide mesh to support multiple configurations with a more general digital signal processing platform.To show the feasibility of this technique,photonic waveguide meshes in different configurations(square,triangular and hexagonal meshes)are designed to realize optical signal interleaving with arbitrary duty cycles.The digital signal processing(DSP)approach offers an effective pathway for the establishment of a general design platform for the software-defined programmable photonic integrated circuits.The use of well-developed DSP techniques and algorithms establishes a link between optical and electrical signals and makes it convenient to realize the computer-aided design of optics–electronics hybrid systems.

The integrated monitoring system for running parameters of key mining equipment based on condition monitoring technology

An integrated monitoring system for running parameters of key mining equipmenton the basis of condition monitoring technology and modern communication networktechnology was developed.The system consists of a client computer with functions ofsignal acquisition and processing, and a host computer in the central control room.Thesignal acquisition module of the client computer can collect the running parameters fromvarious monitoring terminals in real-time.The DSP high-speed data processing system ofthe main control module can quickly achieve the numerical calculation for the collectedsignal.The signal modulation and signal demodulation are completed by the frequencyshift keying circuit and phase-locked loop frequency circuit, respectively.Finally, the signalis sent to the host computer for logic estimation and diagnostic analysis using the networkcommunication technology, which is helpful for technicians and managers to control therunning state of equipment.

Research on Network-based Integrated Condition Monitoring Unit for Rotating Machinery

In this paper, a network-based monitoring unit for condition monitoring andfault diagnosis of rotating machinery is designed and implemented. With the technology of DSP(Digital signal processing) , TCP/IP, and simultaneous acquisition, a mechanism of multi-process andinter-process communication, the integrating problem of signal acquisition, the data dynamicmanagement and network-based configuration in the embedded condition monitoring system is solved. Itoffers the input function of monitoring information for network-based condition monitoring and afault diagnosis system.

Integration of omics studies indicates that species-dependent molecular mechanisms govern male fertility

Background Comparative and comprehensive omics studies have recently been conducted to provide a comprehensive understanding of the biological mechanisms underlying infertility.However,because these huge omics datasets often contain irrelevant information,editing strategies for summarizing and filtering the data are necessary prerequisite steps for identifying biomarkers of male fertility.Here,we attempted to integrate omics data from spermatozoa with normal and below-normal fertility from boars and bulls,including transcriptomic,proteomic,and metabolomic data.Pathway enrichment analysis was conducted and visualized using g:Profiler,Cytoscape,EnrichmentMap,and AutoAnnotation to determine fertility-related biological functions according to species.Results In particular,gamete production and protein biogenesis-associated pathways were enriched in bull spermatozoa with below-normal fertility,whereas mitochondrial-associated metabolic pathways were enriched in boar spermatozoa with normal fertility.These results indicate that below-normal fertility may be determined by aberrant regulation of protein synthesis during spermatogenesis,and the modulation of reactive oxygen species generation to maintain capacitation and the acrosome reaction governs boar sperm fertility.Conclusion Overall,this approach demonstrated that distinct molecular pathways drive sperm fertility in mammals in a species-dependent manner.Moreover,we anticipate that searching for species-specific signaling pathways may aid in the discovery of fertility-related biomarkers within large omics datasets.

Signal and Power Integrity Challenges for High Density System-on-Package

As the increasing desire for more compact,portable devices outpaces Moore’s law,innovation in packaging and system design has played a significant role in the continued miniaturization of electronic systems.Integrating more active and passive components into the package itself,as the case for system-on-package(SoP),has shown very promising results in overall size reduction and increased performance of electronic systems.With this ability to shrink electrical systems comes the many challenges of sustaining,let alone improving,reliability and performance.The fundamental signal,power,and thermal integrity issues are discussed in detail,along with published techniques from around the industry to mitigate these issues in SoP applications.

Software/Hardware Integrating Model of Embedded Reconfigurable Computing System for Digital Signal Processing

The system model of embedded reconfigurable computing system is discussed firstly,followed by the construction of the software and hardware platform.Based on the platform,the function unit and control models of digital signal processing tasks are described.According the functional unit model and control model,a software/hardware integrating model with dataflow control infrastructure is constructed.The software/hardware partition is carried out in the experiments of ultrasonic signal processing,and the results show that the system model is practicable.

Study on key technologies of GNSS-based train state perception for traincentric railway signaling

The application of Global Navigation Satellite Systems(GNSSs)in the intelligent railway systems is rapidly developing all over the world.With the GNSs-based train positioning and moving state perception,the autonomy and flexibility of a novel train control system can be greatly enhanced over the existing solutions relying on the track-side facilities.Considering the safety critical features of the railway signaling applications,the GNSS stand-alone mode may not be sufficient to satisfy the practical requirements.In this paper,the key technologies for applying GNSS in novel train-centric railway signaling systems are investigated,including the multi-sensor data fusion,Virtual Balise(VB)capturing and messaging,train integrity monitoring and system performance evaluation.According to the practical characteristics of the novel train control system under the moving block mode,the details of the key technologies are introduced.Field demonstration results of a novel train control system using the presented technologies under the practical railway operation conditions are presented to illustrate the achievable performance feature of autonomous train state perception using BeiDou Navigation Satellite System(BDS)and related solutions.It reveals the great potentials of these key technologies in the next generation train control system and other GNSS-based railway implementations.

都市圈轨道交通多网融合的信号系统方案研究

结合都市圈网络化、公交化特点,提出都市圈轨道交通信号系统融合的技术目标,即采用现有技术C2/C2+ATO与CBTC系统的融合以及采用新型互联互通技术的信号系统间的融合。分析现有信号主流的CTCS系统与CBTC系统的地面和车载设备在技术层面、工作机理、系统功能等差异的基础上提出“地对车兼容”和“车对地兼容”的互联互通方案,结合各目前相关单位在开展的研究情况,分析新型市域铁路互联互通技术路线。

新工科背景下专业实习体系探索与实践——以轨道交通信号与控制专业为例

为适应新工科对人才培养的新要求,针对轨道交通信号与控制专业的实习体系在实践过程中存在的主要问题,通过深化产教融合、强化虚实结合、细化考评综合,建立纵向“三阶段”逐级递进、横向“双形式”互补的实习新模式,形成“一平台,双引擎,三大素质”的实习教学新理念,构建“一核心,三过程,九指标”的综合能力评价新机制。在实习组织形式、实习资源整合、实习评价方式等方面进行积极探索和大胆实践,对其他工科专业的实习教学体系建立有一定借鉴和参考意义。

四网融合的信号系统方案研究

城市轨道交通、市域(郊)铁路和城际铁路建设均在向网络化运营推进,各种轨道交通之间的互联互通已经是工程建设必须面临的问题,而信号系统的兼容性则是实现各种轨道交通互联互通的一个基础条件。目前,CBTC系统和CTCS列控系统在各自轨道交通领域均处于统治性的市场地位,两种列控系统对土建条件的需求截然不同,由此导致工程规模、功能定位等各方面的重大差异。重点论述两种列控系统在运营能力、公交化运营等方面的差异,针对目前互联互通所面临的技术难点,提出解决方案,为后续工程设计提供一个可行的思路。

光储备池通感一体通信噪声抑制方案(封面文章·特邀)

通感一体技术可利用已大范围铺设的光纤网实现智能传感,具备智能识别、信息融合优势。在同波长通感共传信道中,通感信号串扰及光纤传输噪声严重影响通信信号质量,需提升发射功率来保证低误码率,但增大了非线性噪声和功耗。提出了基于光储备池计算神经网络的通感共传信道通信失真均衡方法,搭建了信道均衡仿真系统,实现了低发射功率下前向通信信号噪声均衡。周期线性调频光和56 Gb/s四电平脉冲幅度调制信号分别用于分布式瑞利散射传感和数据通信。仿真结果表明,在5~14 dBm入纤功率时,与未均衡信号相比该方法可提供高于3个数量级以上的误码率优化。此方法在短距离模块间15~24 km光纤传输和0.5~2.0 GHz带宽线性调频感测脉冲下均实现了2个数量级以上的误码率下降。依托光储备池计算芯片的低能耗和低延时优势,该方法可为进一步提升通感一体系统中噪声抑制能力提供理论支撑。

基于惠更斯等效原理的高速高密度PCB分级建模方法

当带宽达到20 GHz以上时(波长从15 mm到无穷小),高速高密度电子系统的内部电路电磁环境变得十分复杂,越来越难以建模和分析预测,进一步当带宽达到40 GHz以上时,电路电磁环境问题将变得更加尖锐.为在设计阶段能够对电磁作用过程、作用效应进行预测评估及控制,需要精确的建模方法和针对大尺度问题的快速计算技术,尤其是超大带宽超高速混合电路和集成电路.本文提出了基于混合电路环境电磁计算基础理论的跨尺度处理技术,通过惠更斯等效原理和电磁奇异性几何结构的电磁收敛降速机理的利用,解决了多维交叉多尺度电路电磁环境场路融合的高效率高精度建模技术挑战;利用惠更斯等效原理和基尔霍夫积分方程,在区域边界面上定义了惠更斯端口,提出了对于任意复杂印制电路板(Printed Circuit Board,PCB)同时垂直方向和水平方向区域分解的一般方法,实现了任意PCB结构的分级分类处理和模块化封装,提高了高速高密度电子系统分析的灵活度;提出了基于几何结构电磁学多重本地本征展开的技术途径,发展了基于模式和区域分解分割的快速并行处理技术,通过电磁锐变区域本征描述及场分布的本征基函数表征,实现了高精度和高计算速度兼得,减少了复杂电子系统的计算时间和设计时间.统计数据表明,本文提出的方法在0~40 GHz大带宽频率范围内频偏误差为3.7%、幅度偏差为±3 dB.本文提出的PCB分级建模分析方法可以应用于高端电子通信系统设计,提升我国宽带高速数模系统的高效电路设计和环境电磁调控能力,缩短产品研发周期.