两种离子型铱配合物的合成、光物理性质及对OH^(-)的检测

以两种2,2’-联吡啶衍生物为中性配体,2-(3-氟苯基)吡啶为环金属配体,合成了两种新型的离子型Ir(Ⅲ)配合物Ir1和Ir2。利用核磁、质谱和晶体结构分析确认了配合物Ir1和Ir2的结构。配合物Ir1和Ir2在CH_(2)Cl_(2)溶液中的最大发射波长分别为570和528 nm,为橙红光和黄光。两种配合物在无氧CH_(2)Cl_(2)溶液中的量子产率分别为0.88和0.57,磷光寿命分别为2.43和2.40μs。通过理论计算详细讨论了中性配体的取代基种类对其光谱性质的影响。在配合物Ir1的DMSO/H_(2)O溶液中加入OH^(-)后,其发射峰强度提高了近100倍,溶液的发光颜色由红色变为明亮的绿色,检出限为8.47×10^(-6)mol/L。机理研究表明,OH^(-)取代了Ir1的中性配体上的溴取代基,形成了新的含有羟基的配合物,从而改变了Ir1的光谱特性,实现对OH^(-)的高选择性和高灵敏度检测。

A New Characterization of Simple K_3-groups

In this paper, We show that the simple K\-3-groups can be characterized by the orders of their maximal abelian subgroups. That is, we have Theorem Let G be a finite group and M a simple K \-3-group. Then G is isomorphic to M if and only if the set of the orders of the maximal abelian subgoups of G is the same as that of M .

MiR-142-3p Regulates ILC1s by Targeting HMGB1 via the NF-κB Pathway in a Mouse Model of Early Pregnancy Loss

Objective Innate lymphoid cells(ILCs)are a class of newly discovered immunocytes.Group 1 ILCs(ILC1s)are identified in the decidua of humans and mice.High mobility group box 1(HMGB1)is predicted to be one of the target genes of miR-142-3p,which is closely related to pregnancy-related diseases.Furthermore,miR-142-3p and HMGB1 are involved in regulating the NF-κB signaling pathway.This study aimed to examine the regulatory effect of miR-142-3p on ILC1s and the underlying mechanism involving HMGB1 and the NF-κB signaling pathway.Methods Mouse models of normal pregnancy and abortion were constructed,and the alterations of ILC1s,miR-142-3p,ILC1 transcription factor(T-bet),and pro-inflammatory cytokines of ILC1s(TNF-α,IFN-γand IL-2)were detected in mice from different groups.The targeting regulation of HMGB1 by miR-142-3p in ILC1s,and the expression of HMGB1 in normal pregnant mice and abortive mice were investigated.In addition,the regulatory effects of miR-142-3p and HMGB1 on ILC1s were detected in vitro by CCK-8,Annexin-V/PI,ELISA,and RT-PCR,respectively.Furthermore,changes of the NF-κB signaling pathway in ILC1s were examined in the different groups.For the in vivo studies,miR-142-3p-Agomir was injected in the uterus of abortive mice to evaluate the abortion rate and alterations of ILC1s at the maternal-fetal interface,and further detect the expression of HMGB1,pro-inflammatory cytokines,and the NF-κB signaling pathway.Results The number of ILC1s was significantly increased,the level of HMGB1 was significantly upregulated,and that of miR-142-3p was considerably downregulated in the abortive mice as compared with the normal pregnant mice(all P<0.05).In addition,miR-142-3p was found to drastically inhibit the activation of the NF-κB signaling pathway(P<0.05).The number of ILC1s and the levels of pro-inflammatory cytokines were significantly downregulated and the activation of the NF-κB signaling pathway was inhibited in the miR-142-3p Agomir group(all P<0.05).Conclusion miR-142-3p can regulate ILC1s by targeting HMGB1 via the NF-κB signaling pathway,and attenuate the inflammation at the maternal-fetal interface in abortive mice.

香蕉14-3-3蛋白基因Ma-14-3-3d的克隆及序列分析(英文)

[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone 14-3-3 gene from banana; then the amplified sequence was sequenced and homologically analyzed. [Result] A new cDNA homologous with 14-3-3 protein genes were obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ) approaches. The full length of this cDNA was 866 bp encoding 197 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with 14-3-3 protein genes from other plants, and was designated as Musa acuminata 14-3-3 gene (Ma-14-3-3d). Phylogenetic analysis reveals that Ma-14-3-3d has closer genetic relationship with those from monocotyledon species than those from other species. [Conclusion] Ma-14-3-3d belongs to the same lineage of 14-3-3 from monocotyledon.

胃癌组织高迁移率族蛋白A2与基质金属蛋白酶-9表达与肿瘤侵袭转移的关系及预后意义

目的探讨胃癌组织中高迁移率族蛋白A2(HMGA2)及基质金属蛋白酶-9(MMP-9)表达与肿瘤侵袭和转移能力的关系及预后意义。方法采用免疫组化法(SP法)检测93例胃癌组织、30例正常胃黏膜组织中HMGA2、MMP-9的表达;收集患者临床病历资料,并进行随访。结果 HGMA2蛋白在胃癌组织中阳性表达率分别为明显高于正常胃黏膜(P<0.01);MMP-9蛋白在胃癌组织中阳性表达率明显高于正常胃黏膜(P<0.01)。HMGA2与MMP-9在胃癌组织中的蛋白阳性表达均与胃癌组织的肿瘤浸润深度、肿瘤分化程度、淋巴结转移、TNM分期有关(P<0.05);与患者的性别、年龄、肿瘤直径无关(P>0.05)。相关性分析发现,HMGA2与MMP-9在胃癌组织中的表达情况呈正相关(r=0.317,P<0.01)。经Kaplan-Meier生存分析显示,HMGA2、MMP-9蛋白表达阳性患者的生存率均低于表达阴性患者(P<0.01)。结论 HMGA2、MMP-9与胃癌浸润和转移有关,两者表达具有相互协同作用,对胃癌的侵袭和转移起重要的促进作用。HMGA2、MMP-9是影响预后的危险因素,可能成为胃癌治疗的新靶点。

丙泊酚对急性肺损伤大鼠HMGB-1表达的影响

目的探讨丙泊酚的肺保护作用及其可能作用机制。方法采用尾静脉注射内毒素建立急性肺损伤模型,丙泊酚和锌原卟啉同样经过尾静脉给药。将Wistar大鼠按随机数字表法分为实验对照组(C组)、脂多糖组(L组)、脂多糖+丙泊酚组(LP组)。C组尾静脉注射生理盐水;L组尾静脉注射LPS 7.5mg·kg-1;LP组尾静脉注射LPS 7.5mg·kg-1,同时静脉注射丙泊酚30mg·kg-1。给药前及开始后第3、6和12h测定动脉血氧分压(PaO2),实验结束后观察肺湿/干重(W/D)比值、肺组织病理学检查并进行肺损伤轻重程度评分,同时观察各组大鼠肺组织高迁移率族蛋白1(HMGB-1)含量。结果实验前各组PaO2无明显差异,注射LPS后L组PaO2持续下降,和C组相比其PaO2显著降低(P<0.05)。实验结束后L组和C组相比其W/D比值、肺组织病理学检查评分、HO-1及HMGB-1含量显著增加(P<0.05)。而LP组和L组相比其PaO2显著改善,而W/D比值、肺组织病理学检查评分及HMGB-1含量显著降低(P<0.05)。结论丙泊酚具有肺保护作用,且该作用可能与其抑制HMGB-1过度表达作用相关。

电化学法处理循环冷却水的研究进展

循环冷却水系统已广泛应用于工业生产的冷却过程,其显著提高水资源的利用效率。然而,结垢问题直接影响循环冷却系统的稳定性。电化学法可以有效缓解结垢带来的负面问题。因此,本文从两个方面(调控阴极面积,高效分离氢离子和氢氧根)详细阐述电化学法处理循环冷却水的最新研究进展,以更好地利用电化学法处理循环冷却水。

SSH环境下的销售“3-1分栏报表”生成技术与算法研究

针对某集团ERP系统SSH(Strust,Spring,Hibernate)环境下需要研发销售"3-1分栏报表"时遇到的数据操作过于频繁和算法复杂度太高的问题,给出了解决问题的思路,以业务员、区域、客户、产品系列4个分栏使用"Groupby"HQL语句生成List集合,再转换为二维字符串数组,进而给出了算法假设、设计和实现,最终生成数据矩阵,使复杂度降低为O(n3),经测试该方法比"通常方法"约快19倍。

Blood group type antigens in pancreatic intraductal papillary mucinous neoplasms

BACKGROUND: There are few data on blood group(BG) types and types of pancreatic cancers. The aims of this study were to study BG types and BG-antigens in pancreatic intraductal papillary mucinous neoplasms(IPMNs). METHODS: BG type and tumor BG-antigen(glycoprotein) expression(studied by immunohistochemistry on tissue microarrays) were analyzed with regard to characteristics of 101 surgically resected pancreatic IPMNs. RESULTS: Non-O BG type predicted invasive carcinoma independently from high serum CA19-9 and male gender. BG type A was observed more frequently in women than in men. Chronic pancreatitis was more frequently seen in patients with BG type B or AB. Aberrant tumor expression(with regard to BG type) of loss of A antigen expression type occurred in 15.0% of IPMNs and of loss of B antigen expression type in 62.5% of IPMNs. Intraneoplasm BG-antigen expression was not related to dysplasia grade or invasion. CONCLUSION: The results of the study suggest that in pancreatic IPMN, non-O BG type predicted invasive carcinoma, whereas for intratumor BG-antigen expression no specific patterns were detected with regard to the progression of glandular epithelial dysplasia or invasion.

The effect of OH^(-) groups on the spectroscopic properties of erbium-doped tellurite glasses

A series of five different concentration erbium-doped tellurite glasses with various hydroxl groups were prepared. Infrared spectra of glasses were measured. In order to estimate the exact content of OH? groups in samples, various absorption coefficients of the OH? vibration band were analyzed under the different oxygen bubbling times. The absorption spectra of the glasses were measured, and the Judd-Ofelt intensity parameters ?i of samples with the different erbium ions concentration and OH? contents were calculated on the basis of the Judd-Ofelt theory. The peak stimulated emission cross-section of 4I13/2→4I15/2 transition of the samples was finally calculated by using the McCumber theory. The fluorescence spectra of Er3+:4I13/2→4I15/2 transition and the lifetime of Er3+:4I13/2 level of the samples were measured. The effects of OH? groups on the spectroscopic properties of Er3+ doped samples with the different concentrations were discussed. The results showed that the OH? groups had great influences on the Er3+ lifetime and the fluorescence peak intensity. The OH? group is a main influence factor of fluorescence quenching when the doping concentration of Er2O3 is smaller than 1.0 mol%, but higher after this concentration, the energy transfer of Er3+ ions turns into the main function of the fluorescence quenching. And basically, there is no influence on the other spectroscopic properties (FWHM, absorption spectra, peak stimulated emission cross section, etc.).

SYNTHESIS AND CHARACTERIZATION OF POLY{4-[2-(tert-BUTYLDIMETHYLSILOXY)ETHYL]STYRENE}AND ITS HYDROLYSIS DERIVATIVE

The living cationic polymerization of 4-[2-(tert-butyldimethylsiloxy)ethyl]styrene (TBDMES) was studied in methylcyclohexane (MeChx)/methylchloride (MeCl) (50/50 V/V) solvent mixture at -80 degrees C. The initiator 1,1-diphenylethylene (DPE) capped 2-chloro-2,4,4-trimethylpentane (TMPCl) was formed in situ in conjunction with titanium tetrachloride (TiCl(4)). The Lewis acidity of TiCl(4) was decreased by the addition of titanium(IV) isopropoxide (Ti(OiPr)(4)) to accomplish living polymerization of TBDMES. Hydrolysis of poly(TBDMES) in the presence of tetra-butylammonium fluoride yielded poly[4-(2-hydroxyethyl)styrene] (poly(HOES)). FT-IR, NMR and DSC demonstrated the hydrolysis was complete.

New Investigation on the Space Group of Chevkinite

Chevkinite specimen from a rare-earth mineral deposit in Sichuan, southwest of China have been studied in detail by means of transmission electron microscope (TEM).The selected area electron diffraction (SAED)and convergent beam electron diffraction (CBED) patterns , obtained from different crystal zone axis direction , proved coincidently that the space group of chevkinite is C2/m . Fringe lattice image observation showed the mineral crystal is structurally uniform in microscale , and it is an ideal specimen for electron diffraction analysis . The mineral studied here is similar to the one from Bayan Obo , Inner Mongolia , China , in chemical composition and REE distribution . The chemical formula of the crystal is (Ce , La ,… , Ca) 4 Fe2+ (Fe, Ti, Nb) 2 O8 (Si2O7)2 From our study , we come to the conclusion that the space group of the natural chevkinite is C2/m , instead of P21/a as synthetic one . Up to now , chevkinite compositionally similar to the synthetic one , in which the complete replacements of Ce , La by Nd and Fe by Mg or Co occur , has not been discovered in nature . So we suggest that the name for synthesized chevkinite should be further discussed .

STUDY ON PERSISTENT INFECTION OF COXSACKIEVIRUSGROUP B IN MURINE MODEL OF CHRONICVIRAL MYOCARDITIS

Objective To investigate the dynamic change of coxsackievirus B3 (CVB3) in murine model ofchronic myocarditis and revel the molecular mechanism of the persistent infection of the tirus.Methods Strand - specific RT- PCR(ssRT- PCR), quantitative RT- PCR (qRT - PCR) and multiplexRT- PCR(mRT- PCR). Results The positive strand of CVB3 RNA existed in heart tissue up to 3 monthsalthough its amount decreased by 103～4 folds from acute to chronic phase. The negative strand RNA for virusreplication kept its amount on la moleculars per gram heart tissue. Some conserved areas of virus RNA 5’NTRand 3’NTR were lost in chronic phase. Conclusion The virus kept replication during the whole phase ofmyocarditis and speeded down on chronic period in the status of persistent infection. That may be due to theterminal lose of CVB RNA.

A NEW POLYMER-BOUND 1,2-DIOL AS A PROTECTING AGENT FOR SYMMETRICAL DIALDEHYDE

A novel polymer-bound 1,2-diol, 3-polystyrylsulfonyl-1,2-propanediol (6) had been prepared by the reaction of sodium polystyrylsulfinate with allyl bromide, followed by oxidation and. hydrolysis or directly with 3-chloro-1,2-propanediol in the presence of a phase transfer catalyst, n-tetrabutylammonium iodide. The capacity of resin 6 for terephthaidehyde reached 1.43 mmol/g. The aldehydic groups attached to polymer 6 reacted with hydroxylamine hydrochloride or reduced by sodium borohydride giving p-formylbenzaldoxime (yield: 89%)and p-formyl-benzalcohol (yield: 734%), respectively. The high yields of these polymer-supported reactions showed that the polymer 6 possessed the effective isolation of its reactive sites.

STUDIES ON ORGANIC PEROXIDE/N, NDI(2-α-METHYL-ACRYLOYLOXY PROPYL)-PARA-TOLUIDINE BINARY SYSTEMS

The polymerization of methyl methacrylate (MMA) initiated by organic peroxide and polymerizable aromatic tertiary amine such as N, N-di (2-α-methylacryloyloxy propyl)-p-toluidine (MP)_2PT binary system has been studied. It was found that the (MP)_2PT promotes MMA polymerization, and the kinetics of MMA polymerization fits the radical polymerization rate equation. Based on the ESR studies and the end-group analysis the initiation mechanism is proposed.

Regulated CO adsorption by the electrode with OH^(-) repulsive property for enhancing C–C coupling

Electrochemical CO_(2) reduction driven by renewable electricity is one of the promising strategies to store sus-tainable energy as fuels.However,the selectivity of value-added multi-carbon products remains poor for further application of this process.Here,we regulate CO adsorption by forming a Nafion layer on the copper(Cu)electrode that is repulsive to OH^(-),contributing to enhanced selectivity of CO_(2) reduction to C_(2) products with the suppression of C 1 products.The operando Raman spectroscopy indicates that the local OH^(-)would adsorb on part of active sites and decrease the adsorption of CO.Therefore,the electrode with repulsive to OH^(-)can adjust the concentration of OH^(-),leading to the increased adsorption of CO and enhanced C–C coupling.This work shows that electrode design could be an effective strategy for improving the selectivity of CO_(2) reduction to multi-carbon products.

Influence of functional groups on the self-assembly of liquid crystals

Functional groups in the molecule play an important role in the molecular o rganization process.To reveal the influence of functional groups on the self-assembly at interface,herein,the self-assembly structures of three liquid crystal molecules,which only differ in the functional groups,are explicitly characterized by using scanning tunneling microscopy(STM).The high-resolution STM images demonstrate the difference between the supramolecular assembly structures of three liquid crystal molecules,which attribute to the hydrogen bonding interaction andπ-πstacking interaction between different functional groups.The density functional theory(DFT)results also confirm the influence of these functional groups on the self-assemblies.The effort on the self-assembly of liquid crystal molecules at interface could enhance the understanding of the supramolecular assembly mechanism and benefit the further application of liquid crystals.

Large-signal characterizations of DDR IMPATT devices based on group Ⅲ–Ⅴ semiconductors at millimeter-wave and terahertz frequencies

Large-signal （L-S） characterizations of double-drift region （DDR） impact avalanche transit time （IM- PATT） devices based on group III-V semiconductors such as wurtzite （Wz） GaN, GaAs and InP have been carried out at both millimeter-wave （mm-wave） and terahertz （THz） frequency bands. A L-S simulation technique based on a non-sinusoidal voltage excitation （NSVE） model developed by the authors has been used to obtain the high frequency properties of the above mentioned devices. The effect of band-to-band tunneling on the L-S properties of the device at different mm-wave and THz frequencies are also investigated. Similar studies are also carried out for DDR IMPATTs based on the most popular semiconductor material, i.e. Si, for the sake of comparison. A compara- tive study of the devices based on conventional semiconductor materials （i.e. GaAs, InP and Si） with those based on Wz-GaN shows significantly better performance capabilities of the latter at both mm-wave and THz frequencies.

Refraction characteristics of phononic crystals

Some of the most interesting refraction prop- erties of phononic crystals are revealed by examining the anti-plane shear waves in doubly periodic elastic composites with unit cells containing rectangular and/or elliptical multi- inclusions. The corresponding band structure, group velocity, and energy-flux vector are calculated using a powerful mixed variational method that accurately and efficiently yields all the field quantities over multiple frequency pass-bands. The background matrix and the inclusions can be anisotropic, each having distinct elastic moduli and mass densities. Equifrequency contours and energy-flux vectors are read- ily calculated as functions of the wave-vector components. By superimposing the energy-flux vectors on equifrequency contours in the plane of the wave-vector components, and supplementing this with a three-dimensional graph of the corresponding frequency surface, a wealth of information is extracted essentially at a glance. This way it is shown that a composite with even a simple square unit cell con- taining a central circular inclusion can display negative or positive energy and phase velocity refractions, or simply performs a harmonic vibration （standing wave）, depending on the frequency and the wave-vector. Moreover, that the same composite when interfaced with a suitable homoge- neous solid can display： （1） negative refraction with negative phase velocity refraction; （2） negative refraction with pos- itive phase velocity refraction; （3） positive refraction with negative phase velocity refraction; （4） positive refraction with positive phase velocity refraction; or even （5） completereflection with no energy transmission, depending on the fre- quency, and direction and the wavelength of the plane-wave that is incident from the homogeneous solid to the interface. For elliptical and rectangular inclusion geometries, analyti- cal expressions are given for the key calculation quantities. Expressions for displacement, velocity, linear momentum, strain, and stress components, as well as the energy-flux and group velocity components are given in series form. The general results are illustrated for rectangular unit cells, one with two and the other with four inclusions, although any number of inclusions can be considered. The energy-flux and the accompanying phase velocity refractions at an inter- face with a homogeneous solid are demonstrated. Finally, by comparing the results of the present solution method with those obtained using the Rayleigh quotient and, for the lay- ered case, with the exact solutions, the remarkable accuracy and the convergence rate of the present solution method are demonstrated.

A nearly analytic exponential time difference method for solving 2D seismic wave equations

In this paper, we propose a nearly analytic exponential time difference （NETD） method for solving the 2D acoustic and elastic wave equations. In this method, we use the nearly analytic discrete operator to approximate the high-order spatial differential operators and transform the seismic wave equations into semi-discrete ordinary differential equations （ODEs）. Then, the converted ODE system is solved by the exponential time difference （ETD） method. We investigate the properties of NETD in detail, including the stability condition for 1-D and 2-D cases, the theoretical and relative errors, the numerical dispersion relation for the 2-D acoustic case, and the computational efficiency. In order to further validate the method, we apply it to simulating acoustic/elastic wave propagation in mul- tilayer models which have strong contrasts and complex heterogeneous media, e.g., the SEG model and the Mar- mousi model. From our theoretical analyses and numerical results, the NETD can suppress numerical dispersion effectively by using the displacement and gradient to approximate the high-order spatial derivatives. In addition, because NETD is based on the structure of the Lie group method which preserves the quantitative properties of differential equations, it can achieve more accurate results than the classical methods.