Neonatal overfeeding in mice aggravates the development of methionine and choline-deficient diet-induced steatohepatitis in adulthood

Overfeeding in early life is associated with obesity and insulin resistance in adulthood.In the present study,a well-characterized mouse model was used to investigate whether neonatal overfeeding increases susceptibility to the development of non-alcoholic steatohepatitis(NASH)following feeding with a methionine and choline-deficient(MCD)diet.Neonatal overfeeding was induced by adjusting litters to 3 pups per dam(small litter size,SL)in contrast to 10 pups per dam as control(normal litter size,NL).At 11 weeks of age,mice were fed with standard(S)or a methionine and choline-deficient(MCD)diet for 4 weeks.Glucose tolerance tests,tissue staining with haematoxylin and eosin,oil-red O and immunohistochemistry for F4/80,reverse transcription-quantitative polymerase chain reaction(RT-qPCR)and western blotting were performed.Compared with NL mice,SL mice exhibited higher body weight gain from 2 weeks of age throughout adulthood,and more profound glucose intolerance as adults.Sterol regulatory element-binding protein 1c and fatty acid synthase mRNA expression levels in liver were upregulated in SL mice at 3 weeks of age.MCD diet induced typical NASH,especially in SL-MCD mice,evidenced by marked fat accumulation,macrovescular steatosis,ballooned hepatocytes,inflammatory cells infiltration and tumour necrosis factor-a mRNA upregulation in the liver,as well as increased alanine aminotransferase and aspartate aminotransferase levels in the serum.There were no significant differences in liver fibrosis in all groups.Overfeeding during early life exhibited effect with administration of MCD diet in inducing adverse effects on the metabolic function and in promoting the progression of NASH in mice,possibly mediated through dysregulated lipid metabolism in hepatocytes and aggravated hepatic inflammation.

Prepartum dietary energy intake alters adipose tissue transcriptome profiles during the periparturient period in Holstein dairy cows

Background:The aim of the study was to investigate the effect of energy overfeeding during the dry period on adipose tissue transcriptome profiles during the periparturient period in dairy cows.Methods:Fourteen primiparous Holstein cows from a larger cohort receiving a higher-energy diet(1.62 Mcal of net energy for lactation/kg of dry matter;15%crude protein)for ad libitum intake to supply 150%(OVR)or 100%(CTR)of energy requirements from dry off until parturition were used.After calving,all cows received the same lactation diet.Subcutaneous adipose tissue(SAT)biopsies were collected at−14,1,and 14d fromparturition(d)and used for transcriptome profiling using a bovine oligonucleotide microarray.Data mining of differentially expressed genes(DEG)between treatments and due to sampling time was performed using the Dynamic Impact Approach(DIA)and Ingenuity Pathway Analysis(IPA).Results:There was a strong effect of over-feeding energy on DEG with 2434(False discovery rate-corrected P<0.05)between OVR and CTR at−14 d,and only 340 and 538 at 1 and 14 d.The most-impacted and activated pathways in the Kyoto Encyclopedia of Genes and Genomes(KEGG)database that were highlighted by DIA analysis at−14 d in OVR vs.CTR included 9 associated with carbohydrate metabolism,with‘Pyruvate metabolism’,‘Glycolysis/gluconeogenesis’,and‘Pentose phosphate pathway’among the most-activated.Not surprisingly,OVR led to marked activation of lipid metabolism(e.g.‘Fatty acid biosynthesis’and‘Glycerolipid metabolism’).Unexpected metabolic pathways that were activated at−14 d in OVR included several related to metabolism of amino acids(e.g.branched chain)and of cofactors and vitamins(thiamin).Among endocrine and immune system pathways,at−14 d OVR led to marked activation of‘PPAR signalling’and‘Antigen processing and presentation’.Among key pathways affected over time in OVR,a number were related to translation(e.g.mTOR signaling),endocrine/immune signaling(CXCR4 and IGF1),and lipid metabolism(oxidative phosphorylation)with greater activation in OVR vs.CTR specifically at−14 d.Although statistical differences for several pathways in OVR vs.CTR nearly disappeared at 1 and 14 vs.−14 d,despite the well-known catabolic state of adipose depots after calving,the bioinformatics analyses suggested important roles for a number of signaling mechanisms at−14 vs.14 than 1 vs.-14 d.This was particularly evident in cows fed to meet predicted energy requirements during the dry period(CTR).Conclusions:Data underscored a strong activation by overfeeding energy of anabolic processes in the SAT exclusively prepartum.The study confirmed that higher-energy diets prepartum drive a transcriptional cascade of events orchestrated in part by the activation of PPARγthat regulate preadipocyte differentiation and lipid storage in SAT.Novel aspects of SAT biology to energy overfeeding or change in physiologic state also were uncovered,including the role of amino acid metabolism,mTOR signaling,and the immune system.

STUDY OF STRUCTURE AND RIGIDITY OF AIR-JET TEXTURED YARN(ATY)

This paper deals with the structure of air-jet textured yarn(ATY),especilly its core structurein dedail.It is described that overfeed of supply yarn does influence the ATY structure.Then thebending rigidity of ATY has been measured and analyzed.The results shows that various supplyyarns affect the core structure and bending rigidity of ATY.

Absorption and Permeability of Air-jet Textured Glass Fiber Yarn and Its Fabric for Resin

The Absorption and permeability of air-jet textured glass fiber yarn and its fabric and bonded tenacity of the fabric have been studied in relation to the main parameters, air supplied pressure and overfeed ratio.It has been observed that increasing the level of main parameters improves the coated ratio of the textured yarn and its fabric as well as the bonded tenacity of the fabric made of textured yarn. Comparison of absorption or permeability between textured and supplied yarns, and comparison of absorption or permeability and bonded tenacity between the two kinds of fabrics have been elucidated.

The influence of metabolic imbalances and oxidative stress on the outcome of critically ill polytrauma patients: a review

The critically ill polytrauma patient presents with a series of associated pathophysiologies secondary to the traumatic injuries. The most important include systemic inflammatory response syndrome (SIRS), sepsis, oxidative stress (OS), metabolic disorders, and finally multiple organ dysfunction syndrome (MODS) and death. The poor outcome of these patients is related to the association of the aforementioned pathologies. The nutrition of the critically ill polytrauma patient is a distinct challenge because of the rapid changes in terms of energetic needs associated with hypermetabolism, sepsis, SIRS, and OS. Moreover, it has been proven that inadequate nutrition can prolong the time spent on a mechanical ventilator and the length of stay in an intensive care unit (ICU). A series of mathematical equations can predict the energy expenditure (EE), but they have disadvantages, such as the fact that they cannot predict the EE accurately in the case of patients with hypermetabolism. Indirect calorimetry (IC) is another method used for evaluating and monitoring the energy status of critically ill patients. In this update paper, we present a series of pathophysiological aspects associated with the metabolic disaster affecting the critically ill polytrauma patient. Furthermore, we present different non-invasive monitoring methods that could help the intensive care physician in the adequate management of this type of patient.