A series of modified poly(methyl methacrylate) adsorbents were prepared and their adsorption abilities to endotoxin,which is a component of the outer membrance of Gram-negative bacteria,were compared. The results show...A series of modified poly(methyl methacrylate) adsorbents were prepared and their adsorption abilities to endotoxin,which is a component of the outer membrance of Gram-negative bacteria,were compared. The results showed that the adsorbent modified by dimethylamine ligand with a long spacer had the best capacity for adsorbing endotoxin. The adsorbent P-N4,having a hydroxyl group at β -site of the ligand,could form the hydrogen bond with phosphate group. The electrostatic interaction,hydrogen bond and cooperative effect of octatomic ring worked simultaneously. All these factors are responsible for the strong interactions between endotoxin molecule and the adsorbent.展开更多
Endotoxin is lipopolysaccharide(LPS) derived from the cell membrane of gram-negative bacteria. The concentration of endotoxin in endotoxemia patients′ blood increases quickly and leads to severe sepsis, leading to se...Endotoxin is lipopolysaccharide(LPS) derived from the cell membrane of gram-negative bacteria. The concentration of endotoxin in endotoxemia patients′ blood increases quickly and leads to severe sepsis, leading to severe hypertension, cardiovascular continues to have a high failure and death, which is a major cause of death in patients and continues to have a high mortality despite appropriate surgery, potent antibiotic and intensive supportive therapy was conducted. Removal of endotoxin duly and effectually from patients′ blood is very important in clinic. Hemoperfusion can clear toxins from patient′s blood by adsorbent, not only small molecular toxins but also large ones in time. In this paper, the hemoperfusion adsorbent for removing endotoxin was prepared. The physical and chemical parameter of the adsorbent is investigated and the surface structure of adsorbent was investigated through ESEM. The influence of weights of adsorbents and adsorbing time on adsorption ability was also studied. The results showed that the adsorbent with lysine as the ligand had good affinity to endotoxin and the best adsorption capacity of it was 253.1 EU/g when the initial concentration is 10 EU/mL. The blood compatibility of the adsorbent was also studied and the results indicated that the adsorbent has clinic application foreground.展开更多
文摘目的探讨oXiris-内毒素吸附技术治疗终末期肾病(end-stage renal disease,ESRD)合并脓毒症的疗效。方法回顾性收集2020年1月1日—2023年7月30日于河南科技大学第一附属医院住院治疗的ESRD合并脓毒症患者41例,其中oXiris组22例,连续性静脉-静脉血液滤过(continuous veno-venous hemofiltration,CVVH)联合血液灌流(hemoperfusion,HP)组19例。比较2组患者住院死亡率、ICU住院时长、机械通气时长、连续性肾脏替代治疗时长、机械通气患者数、使用滤器数以及在血液净化治疗开始时及治疗后急性生理与慢性健康评分(acute physiology and chronic health evaluation,APACHE-Ⅱ)、去甲肾上腺素(norepinephrine,NE)用量以及实验室检查结果。结果oXiris组患者机械通气时长少于CVVH+HP组(Z=-3.749,P=0.001)。治疗后oXiris组APACHE-Ⅱ评分(t=-4.481,P=0.001)、NE用量(t=-2.036,P=0.049)、C反应蛋白(Z=-2.850,P=0.004)、白细胞介素-6(Z=-2.512,P=0.012)水平较CVVH+HP组降低。结论oXiris-内毒素吸附技术能有效降低ESRD合并脓毒症患者炎症介质,且效果优于CVVH+HP,具有良好的临床应用价值。
文摘A series of modified poly(methyl methacrylate) adsorbents were prepared and their adsorption abilities to endotoxin,which is a component of the outer membrance of Gram-negative bacteria,were compared. The results showed that the adsorbent modified by dimethylamine ligand with a long spacer had the best capacity for adsorbing endotoxin. The adsorbent P-N4,having a hydroxyl group at β -site of the ligand,could form the hydrogen bond with phosphate group. The electrostatic interaction,hydrogen bond and cooperative effect of octatomic ring worked simultaneously. All these factors are responsible for the strong interactions between endotoxin molecule and the adsorbent.
文摘Endotoxin is lipopolysaccharide(LPS) derived from the cell membrane of gram-negative bacteria. The concentration of endotoxin in endotoxemia patients′ blood increases quickly and leads to severe sepsis, leading to severe hypertension, cardiovascular continues to have a high failure and death, which is a major cause of death in patients and continues to have a high mortality despite appropriate surgery, potent antibiotic and intensive supportive therapy was conducted. Removal of endotoxin duly and effectually from patients′ blood is very important in clinic. Hemoperfusion can clear toxins from patient′s blood by adsorbent, not only small molecular toxins but also large ones in time. In this paper, the hemoperfusion adsorbent for removing endotoxin was prepared. The physical and chemical parameter of the adsorbent is investigated and the surface structure of adsorbent was investigated through ESEM. The influence of weights of adsorbents and adsorbing time on adsorption ability was also studied. The results showed that the adsorbent with lysine as the ligand had good affinity to endotoxin and the best adsorption capacity of it was 253.1 EU/g when the initial concentration is 10 EU/mL. The blood compatibility of the adsorbent was also studied and the results indicated that the adsorbent has clinic application foreground.
