Large-field objective lens for multi-wavelength microscopy at mesoscale and submicron resolution

Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view,typically around 1 mm.This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample,such as different brain areas.In addition,conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo.Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm,a numerical aperture of 0.5,and a working wavelength range from 400 to 1000 nm.We achieved a resolution of 0.74μm in fluorescent beads imaging.The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system,a confocal laser scanning system,and a two-photon imaging system.This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution.

Performance in the Fundamentals of Laparoscopic Surgery: Does it reflect global rating scales in the Objective Structured Assessment of Technical Skills in porcine laparoscopic surgery?

Objective:To correlate the utility of the Fundamentals of Laparoscopic Surgery(FLS)manual skills program with the Objective Structured Assessment of Technical Skills(OSATS)global rating scale in evaluating operative performance.Methods:The Asian Urological Surgery Training and Educational Group(AUSTEG)Laparoscopic Upper Tract Surgery Course implemented and validated the FLS program for its usage in laparoscopic surgical training.Delegates’basic laparoscopic skills were assessed using three different training models(peg transfer,precision cutting,and intra-corporeal suturing).They also performed live porcine laparoscopic surgery at the same workshop.Live surgery skills were assessed by blinded faculty using the OSATS rating scale.Results:From March 2016 to March 2019,a total of 81 certified urologists participated in the course,with a median of 5 years of post-residency experience.Although differences in task time did not reach statistical significance,those with more surgical experience were visibly faster at completing the peg transfer and intra-corporeal suturing FLS tasks.However,they took longer to complete the precision cutting task than participants with less experience.Overall OSATS scores correlated weakly with all three FLS tasks(peg transfer time:r=0.331,r^(2)=0.110;precision cutting time:r=0.240,r^(2)=0.058;suturing with intracorporeal knot time:r=0.451,r^(2)=0.203).Conclusion:FLS task parameters did not correlate strongly with OSATS globing rating scale performance.Although FLS task models demonstrated strong validity,it is important to assimilate the inconsistencies when benchmarking technical proficiency against real-life operative competence,as evaluated by FLS and OSATS,respectively.

Distributed Stochastic Optimization with Compression for Non-Strongly Convex Objectives

We are investigating the distributed optimization problem,where a network of nodes works together to minimize a global objective that is a finite sum of their stored local functions.Since nodes exchange optimization parameters through the wireless network,large-scale training models can create communication bottlenecks,resulting in slower training times.To address this issue,CHOCO-SGD was proposed,which allows compressing information with arbitrary precision without reducing the convergence rate for strongly convex objective functions.Nevertheless,most convex functions are not strongly convex(such as logistic regression or Lasso),which raises the question of whether this algorithm can be applied to non-strongly convex functions.In this paper,we provide the first theoretical analysis of the convergence rate of CHOCO-SGD on non-strongly convex objectives.We derive a sufficient condition,which limits the fidelity of compression,to guarantee convergence.Moreover,our analysis demonstrates that within the fidelity threshold,this algorithm can significantly reduce transmission burden while maintaining the same convergence rate order as its no-compression equivalent.Numerical experiments further validate the theoretical findings by demonstrating that CHOCO-SGD improves communication efficiency and keeps the same convergence rate order simultaneously.And experiments also show that the algorithm fails to converge with low compression fidelity and in time-varying topologies.Overall,our study offers valuable insights into the potential applicability of CHOCO-SGD for non-strongly convex objectives.Additionally,we provide practical guidelines for researchers seeking to utilize this algorithm in real-world scenarios.

Effect of low-degree astigmatism on the objective visual quality

AIM:To evaluate the effect of low-degree astigmatism on objective visual quality through the Optical Quality Analysis System(OQAS).METHODS:This study enrolled 46 participants(aged 23 to 30y,90 eyes)with normal or corrected-to-normal vision.The cylindrical lenses(0,0.5,0.75,1.0,and 1.25 D)were placed at the axial direction(180°,45°,90°,and 135°)in front of the eyes with the best correction to form 16 types of regular low-degree astigmatism.OQAS was used to detect the objective visual quality,recorded as the objective scattering index(OSI),OQAS values at contrasts of 100%,20%,and 9%predictive visual acuity(OV100%,OV20%,and OV9%),modulation transfer function cut-off(MTFcut-off)and Strehl ratio(SR).The mixed effect linear model was used to compare objective visual quality differences between groups and examine associations between astigmatic magnitude and objective visual quality parameters.RESULTS:Apparent negative relationships between the magnitude of low astigmatism and objective visual quality were observed.The increase of OSI per degree of astigmatism at 180°,45°,90°,and 135°axis were 0.38(95%CI:0.35,0.42),0.50(95%CI:0.46,0.53),0.49(95%CI:0.45,0.54)and 0.37(95%CI:0.34,0.41),respectively.The decrease of MTFcut-off per degree of astigmatism at 180°,45°,90°,and 135°axis were-10.30(95%CI:-11.43,-9.16),-12.73(95%CI:-13.62,-11.86),-12.75(95%CI:-13.79,-11.70),and-9.97(95%CI:-10.92,-9.03),respectively.At the same astigmatism degree,OSI at 45°and 90°axis were higher than that at 0°and 135°axis,while MTFcut-off were lower.CONCLUSION:Low astigmatism of only 0.50 D can significantly reduce the objective visual quality.

Objective Model Selection in Physics: Exploring the Finite Information Quantity Approach

Traditional methods for selecting models in experimental data analysis are susceptible to researcher bias, hindering exploration of alternative explanations and potentially leading to overfitting. The Finite Information Quantity (FIQ) approach offers a novel solution by acknowledging the inherent limitations in information processing capacity of physical systems. This framework facilitates the development of objective criteria for model selection (comparative uncertainty) and paves the way for a more comprehensive understanding of phenomena through exploring diverse explanations. This work presents a detailed comparison of the FIQ approach with ten established model selection methods, highlighting the advantages and limitations of each. We demonstrate the potential of FIQ to enhance the objectivity and robustness of scientific inquiry through three practical examples: selecting appropriate models for measuring fundamental constants, sound velocity, and underwater electrical discharges. Further research is warranted to explore the full applicability of FIQ across various scientific disciplines.

Eclipse计划系统MU Objective工具在鼻咽癌容积调强放射治疗计划中的应用研究

目的:探讨Varian Eclipse计划系统MU Objective工具在鼻咽癌容积调强放射治疗(VMAT)计划中的应用,旨在保证治疗计划质量的同时降低治疗计划的机器跳数(MU)。方法:选取已接受容积调强放射治疗技术的21例鼻咽癌患者,在未使用MU Objective工具的情况下给每例患者设计治疗参考计划(MU_(ref));将使用MU Objective工具对MU_(ref)进行重新优化,分别设置MU_(ref)的MU的30%、50%、70%、90%和120%,分别命名为MU_(30%)、MU_(50%)、MU_(70%)、MU_(90%)和MU_(120%)。将治疗计划MU_(30%)、MU_(50%)、MU_(70%)、MU_(90%)和MU_(120%)分别与MU_(ref)进行配对t检验统计分析,比较两种治疗计划的靶区、危及器官(OAR)剂量分布和计划的MU。结果:使用MU Objective工具对参考计划优化MU后,与参考计划相比部分靶区和OAR剂量体积参数差异虽有统计学意义,但无临床意义。治疗计划参数为MU_(30%)、MU_(50%)、MU_(70%)和MU_(90%)时,与MU_(ref)比较计划MU平均分别减少21.5%、19.5%、16.6%和8%,差异有统计学意义(t=9.652,t=8.843,t=8.963,t=11.323;P<0.05)。在MU_(120%)时,治疗计划MU平均增加1.7%,但未显著提高靶区剂量覆盖。结论:对于鼻咽癌VMAT计划,使用Eclipse计划系统的MU Objective工具在减少计划MU的同时可获得与参考计划相似的剂量分布。MU Objective工具可作为鼻咽癌或其他部位VMAT计划的常规使用工具,以保证计划质量同时减少放射治疗计划的MU。

Objective Identification and Climatic Characteristics of Heavy-Precipitation Northeastern China Cold Vortexes

The northeastern China cold vortex(NCCV)plays an important role in regional rainstorms over East Asia.Using the National Centers for Environmental Prediction Final reanalysis dataset and the Global Precipitation Measurement product,an objective algorithm for identifying heavy-precipitation NCCV(HPCV)events was designed,and the climatological features of 164 HPCV events from 2001 to 2019 were investigated.The number of HPCV events showed an upward linear trend,with the highest frequency of occurrence in summer.The most active region of HPCV samples was the Northeast China Plain between 40°–55°N.Most HPCV events lasted 3–5 days and had radii ranging from 250 to 1000 km.The duration of HPCV events with larger sizes was longer.About half of the HPCV events moved into(moved out of)the definition region(35°–60°N,115°–145°E),and half initiated(dissipated)within the region.The initial position was close to the western boundary of the definition region,and the final position was mainly near the eastern boundary.The locations associated with the precipitation were mostly concentrated within 2000 km southeast of the HPCV systems,and they were farther from the center in the cold season than in the warm season.

Multi‐objective particle swarm optimisation of complex product change plan considering service performance

Design change is an inevitable part of the product development process.This study proposes an improved binary multi‐objective PSO algorithm guided by problem char-acteristics(P‐BMOPSO)to solve the optimisation problem of complex product change plan considering service performance.Firstly,a complex product multi‐layer network with service performance is established for the first time to reveal the impact of change effect propagation on the product service performance.Secondly,the concept of service performance impact(SPI)is defined by decoupling the impact of strongly associated nodes on the service performance in the process of change affect propagation.Then,a triple‐objective selection model of change nodes is established,which includes the three indicators:SPI degree,change cost,and change time.Furthermore,an integer multi‐objective particle swarm optimisation algorithm guided by problem characteristics is developed to solve the model above.Experimental results on the design change problem of a certain type of Skyworth TV verify the effectiveness of the established optimisation model and the proposed P‐BMOPSO algorithm.

Multi-Objective Optimization with Artificial Neural Network Based Robust Paddy Yield Prediction Model

Agriculture plays a vital role in the food production process that occupies nearly one-third of the total surface of the earth.Rice is propagated from the seeds of paddy and it is a stable food almost used byfifty percent of the total world population.The extensive growth of the human population alarms us to ensure food security and the country should take proper food steps to improve the yield of food grains.This paper concentrates on improving the yield of paddy by predicting the factors that influence the growth of paddy with the help of Evolutionary Computation Techniques.Most of the researchers used to relay on historical records of meteorological parameters to predict the yield of paddy.There is a lack in analyzing the day to day impact of meteorological parameters such as direction of wind,relative humidity,Instant Wind Speed in paddy cultivation.The real time meteorological data collected and analysis the impact of weather parameters from the day of paddy sowing to till the last day of paddy harvesting with regular time series.A Robust Optimized Artificial Neural Network(ROANN)Algorithm with Genetic Algorithm(GA)and Multi Objective Particle Swarm Optimization Algorithm(MOPSO)proposed to predict the factors that to be concentrated by farmers to improve the paddy yield in cultivation.A real time paddy data collected from farmers of Tamilnadu and the meteorological parameters were matched with the cropping pattern of the farmers to construct the database.The input parameters were optimized either by using GA or MOPSO optimization algorithms to reconstruct the database.Reconstructed database optimized by using Artificial Neural Network Back Propagation Algorithm.The reason for improving the growth of paddy was identified using the output of the Neural Network.Performance metrics such as Accuracy,Error Rate etc were used to measure the performance of the proposed algorithm.Comparative analysis made between ANN with GA and ANN with MOPSO to identify the recommendations for improving the paddy yield.

Salient Object Detection Based on a Novel Combination Framework Using the Perceptual Matching and Subjective-Objective Mapping Technologies

The integrity and fineness characterization of non-connected regions and contours is a major challenge for existing salient object detection.The key to address is how to make full use of the subjective and objective structural information obtained in different steps.Therefore,by simulating the human visual mechanism,this paper proposes a novel multi-decoder matching correction network and subjective structural loss.Specifically,the loss pays different attentions to the foreground,boundary,and background of ground truth map in a top-down structure.And the perceived saliency is mapped to the corresponding objective structure of the prediction map,which is extracted in a bottom-up manner.Thus,multi-level salient features can be effectively detected with the loss as constraint.And then,through the mapping of improved binary cross entropy loss,the differences between salient regions and objects are checked to pay attention to the error prone region to achieve excellent error sensitivity.Finally,through tracking the identifying feature horizontally and vertically,the subjective and objective interaction is maximized.Extensive experiments on five benchmark datasets demonstrate that compared with 12 state-of-the-art methods,the algorithm has higher recall and precision,less error and strong robustness and generalization ability,and can predict complete and refined saliency maps.

图像引导和点云空间约束的公路洒落物检测定位方法

公路洒落物是影响交通安全的重要因素之一,为了解决中小尺度公路洒落物检测中的漏检、误检以及难以定位等问题,本文提出了一种图像引导和点云空间约束的公路洒落物检测定位方法。该方法使用改进的YOLOv7-OD网络处理图像数据获取二维目标预测框信息,将目标预测框投影到激光雷达坐标系下得到锥形感兴趣区域(region of interest,ROI)。在ROI区域内的点云空间约束下,联合点云聚类和点云生成算法获得不同尺度的洒落物在三维空间中的检测定位结果。实验表明:改进的YOLOv7-OD网络在中尺度目标上的召回率和平均精度分别为85.4%和82.0%,相比YOLOv7网络分别提升6.6和8.0个百分点;在小尺度目标上的召回率和平均精度分别为66.8%和57.3%,均提升5.3个百分点;洒落物定位方面,对于距离检测车辆30~40 m处的目标,深度定位误差为0.19 m,角度定位误差为0.082°,实现了多尺度公路洒落物的检测和定位。

基于改进YOLOX与多级数据关联的行人多目标跟踪算法研究

目标跟踪是计算机视觉领域的基本问题,行人多目标跟踪在智能监控、智慧交通等多个领域有着广泛的应用前景。然而实际跟踪场景中存在频繁遮挡、尺度变化等情况,给多目标跟踪算法带来了极大的挑战。为了进一步提升跟踪精度,在DeepSORT的基础上,提出一种基于改进YOLOX与多级数据关联的行人多目标跟踪算法。对于检测器,为了增强网络的特征表达能力,提高检测精度,在YOLOX骨架网络与颈部网络分别引入ECA通道注意力模块与ASFF自适应特征融合模块。对于身份识别特征,为了减少数据关联步骤的错误匹配数量,提高跟踪效率,使用轻量的OSNet重识别网络与NSA卡尔曼滤波获取目标特征。对于数据关联,为了减少身份切换次数,避免目标丢失,将检测与跟踪都进行分类处理,使用不同的相似性计算方法,实现基于检测置信度与轨迹状态的多级数据关联。实验结果表明:与改进前YOLOX与DeepSORT简单结合的算法相比,在YOLOX中引入ECA模块与ASFF模块使误检数量大幅降低,使用YOLOX-s模型时降幅可达17%;结合OSNet模型与NSA卡尔曼滤波的特征提取方法能提高跟踪稳定性,IDF1指标提高0.77%,IDSW减少947;基于检测置信度与轨迹状态的多级数据关联算法可以明显改善跟踪性能,MOTA指标提升3.36%。算法最终在MOT17与MOT20测试集上的MOTA达80.4%与77.7%,IDF1达78.4%与76.7%。提出的行人多目标跟踪方法相较于其他先进算法在跟踪精度与跟踪速度上达到更好的平衡,可为工业上在线行人多目标跟踪应用提供参考。

CNN-Transformer特征融合多目标跟踪算法

在卷积神经网络(CNN)中,卷积运算能高效地提取目标的局部特征,却难以捕获全局表示;而在视觉Transformer中,注意力机制可以捕获长距离的特征依赖,但会忽略局部特征细节。针对以上问题,提出一种基于CNN-Transformer双分支主干网络进行特征提取和融合的多目标跟踪算法CTMOT(CNN-transformer multi-object tracking)。使用基于CNN和Transformer双分支并行的主干网络分别提取图像的局部和全局特征。使用双向桥接模块(two-way braidge module,TBM)对两种特征进行充分融合。将融合后的特征输入两组并行的解码器进行处理。将解码器输出的检测框和跟踪框进行匹配,完成多目标跟踪任务。在多目标跟踪数据集MOT17、MOT20、KITTI以及UADETRAC上进行评估,CTMOT算法的MOTP和IDs指标在四个数据集上均达到了SOTA效果,MOTA指标分别达到了76.4%、66.3%、92.36%和88.57%,在MOT数据集上与SOTA方法效果相当,在KITTI数据集上达到SOTA效果。由于同时完成目标检测和关联,能够端到端进行目标跟踪,跟踪速度可达35 FPS,表明CTMOT算法在跟踪的实时性和准确性上达到了较好的平衡,具有较大潜力。

层级引导的增强型多目标萤火虫算法

针对多目标萤火虫算法在求解过程中易产生振荡和聚集现象,导致开发能力较弱、求解精度不佳的问题,提出一种层级引导的增强型多目标萤火虫算法(hierarchical guided enhanced multi-objective firefly algorithm,HGEMOFA)。构建层级引导模型,利用非支配排序获得不同层级个体,用优势层个体引导劣势层个体进化,明确引导方向,解决了进化过程中出现的振荡,减少了聚集现象的出现,增强了算法收敛性;引入莱维飞行扰动最优层个体,增强算法的全局搜索能力;每代进化完成后,对当前种群采用变异机制,增强算法的局部开发能力;把变异后的种群和前一代种群合并进行环境选择,筛选出和前一代种群规模相同的子代,避免优势解丢失。实验结果表明:HGEMOFA能有效增强解的收敛性和多样性。

基于LMIENet图像增强的矿井下低光环境目标检测方法

煤矿井下工作环境复杂,存在人造光源亮度低、粉尘多和水气密度大等不利因素,导致现有的目标检测算法在应用到煤矿井下时,存在提取特征困难、目标识别和定位精度低等问题。提出一种煤矿井下低照度环境目标检测算法,由矿井低光图像增强模块LMIENet和目标检测模块组成,使用图像增强模块对原始图像进行画质提升,恢复各类图像信息,再使用目标检测网络对增强图像进行特定目标检测,有效提高检测的精确度。在图像增强模块中,改进Zero-DCE算法设计轻量级增强参数预测网络,计算像素级增强参数矩阵,用于低光照图像的亮度调整和画质增强,该网络通过设计的非参考损失函数隐性衡量图像的增强效果,引导网络进行无监督学习,使网络能够不依赖配对数据集对原始图像进行自适应的画质增强。目标检测模块中,采用YOLO v8n目标检测模型,其轻量化的模型尺寸和高灵活性可避免模型整体复杂度过高;采用Focal-EIoU Loss改进回归损失函数,有效加速模型收敛并提升模型检测精度。实验结果显示:与经典目标检测算法Faster R–CNN,SSD,RetinaNet,FCOS等相比,提出算法在自建矿井人员数据集上表现出色,低光照环境下目标检测的mAP@0.5达到98.0%,mAP@0.5∶0.95达64.8%,在实验环境中单帧图像推理时间仅11 ms,优于其他对比方法,证明提出算法能够有效实现在煤矿井下低照度复杂环境下的目标检测,且耗时短、计算效率高。

基于改进YOLOv8的无人机航拍图像目标检测算法

针对现存无人机航拍图像目标检测算法检测精度较低、模型较为复杂的问题,提出一种改进YOLOv8的目标检测算法。在骨干网络引入多尺度注意力EMA,捕捉细节信息,以提高模型的特征提取能力;改进C2f模块,减小模型的计算量。提出了轻量级的Bi-YOLOv8特征金字塔网络结构改进YOLOv8的颈部,增强了模型多尺度特征融合能力,改善网络对小目标的检测精度。使用WIoU Loss优化原网络损失函数,引入一种动态非单调聚焦机制,提高模型的泛化能力。在无人机航拍数据集VisDrone2019上的实验表明,提出算法的mAP50为40.7%,较YOLOv8s提升了1.5%,参数量降低了42%,同时相比于其他先进的目标检测算法在精度和速度上均有提升,证明了改进算法的有效性和先进性。

边缘信息增强的显著性目标检测网络

针对显著性目标检测任务中识别结果边缘模糊的问题,提出了一种能够充分利用边缘信息增强边缘像素置信度的新模型。该网络主要有两个创新点:设计三重注意力模块,利用预测图的特点直接生成前景、背景和边缘注意力,并且生成注意力权重的过程不增加任何参数;设计边缘预测模块,在分辨率较高的网络浅层进行有监督的边缘预测,并与网络深层的显著图预测融合,细化了边缘。在6种常用公开数据集上用定性和定量的方法评估了该模型,并且与其他模型进行充分对比,证明设计的新模型能够取得最优的效果。此外,该模型参数量为30.28 M,可以在GTX 1080 Ti显卡上达到31帧·s^(-1)的预测速度。

基于局部特征深度信息的绝缘子小样本缺陷检测

基于深度学习的目标检测技术已广泛应用于绝缘子缺陷检测中,然而现有目标检测算法主要基于大量缺陷样本训练网络模型,无法对少样本缺陷进行准确识别。针对绝缘子缺陷检测过程中缺陷样本量不足的问题,该文提出了一种基于局部特征深度信息的绝缘子小样本缺陷检测方法。首先采用旋转目标检测网络改进Faster R-CNN(faster region-based convolutional neural network)模型提取绝缘子串区域,然后对绝缘子串特征进行划分,提取绝缘子串局部特征并基于深度推土距离(deep earth mover’s distance,Deep EMD)网络实现小样本缺陷检测。实验结果表明,在玻璃绝缘子自爆缺陷检测中,所提出方法采用2张训练样本可取得与现有目标检测方法 200张训练样本相同的效果,采用10张训练样本的绝缘子自爆检测在与真值框的交并比阈值为0.5至0.95之间的平均精度(mean average precision,mAP)达到0.65,该方法为小样本电力设备缺陷智能化检测提供了新的方法和思路。

OBE理念下自动化一流本科专业课程教学改革与实践

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