Objective:To determine the antibiotic resistance of Vibrio choleras(V.cholerae) O1 biotype El Tor serotype Ogawa isolates involved in an outbreak of watery diarrhea in Kolkata,and to explore the role of plasmid in med...Objective:To determine the antibiotic resistance of Vibrio choleras(V.cholerae) O1 biotype El Tor serotype Ogawa isolates involved in an outbreak of watery diarrhea in Kolkata,and to explore the role of plasmid in mediating antibiotic resistance.Methods:Antibiotic susceptibility and minimum inhibitory concentration(MIC) values of antibiotics for the isolated V.cholerae 01 Ogawa(n=12) were determined by disk diffusion and agar dilution methods,respectively,using ampicillin(Am),chloramphenicol(C),trimethoprim(Tm),tetracycline(T).erythromycine(Er), nalidixic acid(Nx).ciprofloxacin(Cp),amikacin(Ak) and cefotaxime(Cf).Plasmid curing of multidrug resistant(MDR) V.cholerae 01 Ogawa strains was done following ethidium bromide treatment.Following electrophoresis,the plasmid DNAs,extracted from the isolated MDR V. cholerae O1 Ogawa strains and their cured derivatives,were visualized and documented in ’gel doc’ system.Results:The outbreak causing V.cholerae O1 Ogawa isolates were MDR as determined by disk diffusion susceptibility test,and MIC determination.The isolates showed three different drug resistance patterns:AmTmTErNx(for 6 isolates).TmTErCp(for 5 isolates), and AmTniNx(for one isolate),and showed uniform sensitivity to C,Ak and Cf.The loss of plasmids with the concomitant loss of resistance to Am,Tm,T and Er of the isolates occurred following ethidium bromide treatment.Conclusions:The current findings suggest that the V. cholerae O1 Ogawa associated with the cholera outbreak were MDR,and resistance to Am,Tm,T and Er among the isolates were plasmid mediated.展开更多
于眼症是异基因骨髓移植后慢性移植物抗宿主病（chroic graft versus host disease，cGVHD）最多见的眼部并发症之一。Ogawa等报道，患者一旦出现于眼症状，干眼症的病情发展迅速，虽不危及生命却严重影响生活质量。现将我院门诊随诊的...于眼症是异基因骨髓移植后慢性移植物抗宿主病（chroic graft versus host disease，cGVHD）最多见的眼部并发症之一。Ogawa等报道，患者一旦出现于眼症状，干眼症的病情发展迅速，虽不危及生命却严重影响生活质量。现将我院门诊随诊的1例异基因骨髓移植患者的眼部检查及治疗情况报告如下。展开更多
第一次中日临床疼痛联合研讨会
第一次中日疼痛治疗研讨会于1992年7月27日在日本札幌市（Sapporo）召开，当时是第26回日本疼痛学会在旭川市（Asahikawa）召开之后的卫星会。组织与发起这次会议的是当时日本疼痛学会主席小川秀道教授（...第一次中日临床疼痛联合研讨会
第一次中日疼痛治疗研讨会于1992年7月27日在日本札幌市（Sapporo）召开，当时是第26回日本疼痛学会在旭川市（Asahikawa）召开之后的卫星会。组织与发起这次会议的是当时日本疼痛学会主席小川秀道教授（Hidemichi Ogawa, MD; President.The 26th Congress of Japan Society of Pain Clinicians ; Professsor and Chairman, Department of Anesthesiology and Critical Care Medicine, Asahikawa Medical College）。展开更多
文摘Objective:To determine the antibiotic resistance of Vibrio choleras(V.cholerae) O1 biotype El Tor serotype Ogawa isolates involved in an outbreak of watery diarrhea in Kolkata,and to explore the role of plasmid in mediating antibiotic resistance.Methods:Antibiotic susceptibility and minimum inhibitory concentration(MIC) values of antibiotics for the isolated V.cholerae 01 Ogawa(n=12) were determined by disk diffusion and agar dilution methods,respectively,using ampicillin(Am),chloramphenicol(C),trimethoprim(Tm),tetracycline(T).erythromycine(Er), nalidixic acid(Nx).ciprofloxacin(Cp),amikacin(Ak) and cefotaxime(Cf).Plasmid curing of multidrug resistant(MDR) V.cholerae 01 Ogawa strains was done following ethidium bromide treatment.Following electrophoresis,the plasmid DNAs,extracted from the isolated MDR V. cholerae O1 Ogawa strains and their cured derivatives,were visualized and documented in ’gel doc’ system.Results:The outbreak causing V.cholerae O1 Ogawa isolates were MDR as determined by disk diffusion susceptibility test,and MIC determination.The isolates showed three different drug resistance patterns:AmTmTErNx(for 6 isolates).TmTErCp(for 5 isolates), and AmTniNx(for one isolate),and showed uniform sensitivity to C,Ak and Cf.The loss of plasmids with the concomitant loss of resistance to Am,Tm,T and Er of the isolates occurred following ethidium bromide treatment.Conclusions:The current findings suggest that the V. cholerae O1 Ogawa associated with the cholera outbreak were MDR,and resistance to Am,Tm,T and Er among the isolates were plasmid mediated.
文摘于眼症是异基因骨髓移植后慢性移植物抗宿主病（chroic graft versus host disease，cGVHD）最多见的眼部并发症之一。Ogawa等报道，患者一旦出现于眼症状，干眼症的病情发展迅速，虽不危及生命却严重影响生活质量。现将我院门诊随诊的1例异基因骨髓移植患者的眼部检查及治疗情况报告如下。
文摘第一次中日临床疼痛联合研讨会
第一次中日疼痛治疗研讨会于1992年7月27日在日本札幌市（Sapporo）召开，当时是第26回日本疼痛学会在旭川市（Asahikawa）召开之后的卫星会。组织与发起这次会议的是当时日本疼痛学会主席小川秀道教授（Hidemichi Ogawa, MD; President.The 26th Congress of Japan Society of Pain Clinicians ; Professsor and Chairman, Department of Anesthesiology and Critical Care Medicine, Asahikawa Medical College）。
