Oleuropein-Specific-β-Glucosidase Activity Marks the Early Response of Olive Fruits (Olea europaea) to Mimed Insect Attack

Olive fruits are seriously deteriorated by pre and postharvest damage due to the attack of insects, such as Bactrocera olaea, which strongly alters the quality of olives. Defence response in olive fruits injured both by pathogens and by mechanical damages has been associated with the enzyme β-glucosidase, which specifically hydrolyses oleuropein, producing highly reactive aldehyde molecules. In situ detection of ^-glucosidase activity in olive fruit tissues following injury, which simulates Bactrocera oleae punctures, is reported. The assay was performed in two cultivars showing different degrees of susceptibilities to fly infestation. In both cultivars, the histochemical assay for β-glucosidase showed that within 20 min after the injury, a strong ^-glucosidase activity could be observed in the damaged tissues. Thereafter a progressive enzyme inactivation occurred starting from tissues around the boundary of the injury with decrease of the enzyme activity and stopped after 3 h. Whereas the mass of active cells reached a distance of （300±50） μm from the edge of the injury. Biochemical analyses showed that in extracts of the injured fruit, β-glucosidase activity rapidly increased within 20 min from injury, thereafter decreasing and reaching values comparable with those in intact fruits. Following puncture, the oleuropein contents did not change significantly in the high susceptibility cultivar, whereas it rapidly decreased in the cultivar showing low susceptibility. The results strongly suggest that olive fruits susceptible towards fly infestation could be related to the ability of the oleuropein-degrading-β-glucosidase to produce the highly reactive molecules in the damaged tissues. As a consequence of puncture, high level of peroxidase activity was detected. This feature also suggested that this enzyme could play a key role in the defence response against insect injuries.

Effect of Foliar Spray of Fe, GA3, Cultivars and Their Interactions on Growth of Olive （Olea europaea L.） Transplants cvs. Khithairy and Sorany

The study was conducted at the nursery of Malta station/Duhok. Kurdistan region-lraq, during the season 2011 to study the effect of GA3 and Fe on vegetative growth of two year old of two olive （Olea europaea L.） cultivars （Kithary and Sorany）, the transplant were sprayed with four levels （0, 250, 500 and 750 mg L^-1 GA3） and four levels of chelated iron （0, 25, 50 and 75 mg Fe L^-1）. Foliar application of GA3 at 500 mg Ll increased significantly stem diameter （mm）, leaf number, increasing GA3 level up 750 mg L^-1 significantly increase shoot length （cm） plantl, leaf area. However, 50 mg Fe L^-1 increased significantly leaf number, leaf area, chlorophyll content, and when treated with 75 mg Fe L^-1 significantly increase stem diameter, shoot length cm plant^-1, leaf fresh weight （g）, leaf dry weight （g）. While, Kithary cultivar significant dominated Sorany cultivar of the most studied characteristics. The interactions between GA3, Fe and cultivars significantly affected on the most of the characteristics studied, foliar sprays of Kithary cultivar with 750 mg Ll GA^-1 and 0 mg Fe Ll significantly increase chlorophyll content and leaf area, and Kithary cultivar when treated only by 75 mg Fe Ll increase significantly plant high （cm）, stem diameter （mm）, leaf dry weight （g） and leaf fresh weight when compared with the control.

Transplantation and Post-planting Tending and Management Techniques of Olive Trees(Olea europaea L.)

This paper introduced the preparation work before transplanting big olive trees(Olea europaea L.),including key technical measures such as plant excavation,transportation,planting,and post-planting tending.The aim was to provide scientific basis and feasible solutions for adjusting the density of olive trees in Shiyan City,and to assist in the high-quality development of the olive industry.

Evaluation of the Incidence and Severity of Olive Leaf Spot Caused by <i>Spilocaea oleagina</i>on Olive Trees in Palestine

Olive Leaf Spot (OLS) is found in many parts of the world and cause readuced growth and yield in olive trees. In this study, investigations were carried out to measure the incidence (% infected leaves) and severity (number of lesions/leaf) of OLS in olive growing regions in Palestine, including the regions of Hebron, Bethlehem, Tulkarm, Salfit, Jenin, Nablus, Ramallah and Qalqiyah. OLS was found in all study areas with significantly higher percent incidences in Jenin and Nablus (67.16% ± 18.16% and 46.06% ± 23.70%, respectively). OLS was more severe in Nablus, Qalqilyah, Jenin and Tulkarm (severity grade 3.0 - 3.7). Analysis indicate that there is a positive correlation (R2 = 0.597) between disease incidence and severity. OLS appeared to be particularly severe on trees that were growing in regions with higher annual rainfall. The correlation between severity and rainfall was positive (R2 = 0.543).

Insecticidal Activity of an Indian Botanical Insecticide ULTRA ACT&#174 against the Olive Pest <i>Bactrocera oleae</i>(Diptera: Tephritidae) in Tunisia

Bactrocera oleae is the most economically damaging insect of olive in the Mediterranean. As a reliable alternative to synthetic chemical insecticides, botanical pesticides from plant extracts are also considered natural control methods safe for the environment and human health. In practical applications up to date, studies demonstrating toxically effects of biological control agents on the insect have been carried out in organic farming systems based on some industrial microbial formulations. But much less attention has been given to botanical insecticides against B. oleae. Thus, a certified botanical insecticide issue from various plants and approved per Indian and International organic standards was tested against larvae and pupae of the olive pest Bactrocera oleae in the east of Tunisia under laboratory conditions. The experiments were conducted with 3 normal treatments and 1 control. Two techniques were used for larvae and pupae: direct contact using concentration from 0.5;0.750;1 and 1.5 mg/l and fumigation treatment using 2 mg/l conducted in rectangular boxes (25&#176C - 27&#176C, 75% - 85% RH and 14:10 LD photo period). For larval stages, LC50 and LC90 values for Ultra-Act were 0.45 and 1.22 mg/L in direct contact and 1.5 - 2 mg/l with fumigation, respectively. For pupal stage, the ID25 and ID50 values were estimated to be 0.71 mg and 1.26 mg respectively corresponding to the inhibition of emergence of 25% and 50% of B. oleae adults from pupa. Our results indicate that sprayed Ultra-Act product has the potential to control the three olive fly instar larvae. Pupal emergency was decreased significantly with fumigation treatment. In conclusion, Ultra-Act is a promising botanical insecticide against B. oleae that could be used as a successful alternative for chemicals in integrated control methods of this pest in Tunisia.

Effect of hydro-alcoholic extract of Olea europaea on apoptosis-related genes and oxidative stress in a rat model of torsion/detorsion-induced ovarian damage

Objective: To evaluate the impact of Olea (O.) europaea extract on markers of oxidative stress and apoptosis of ovarian tissues in a rat model of torsion/detorsion-induced ovarian damage. Methods: A total of 28 Wistar female rats were randomly assigned into 4 groups, with 7 rats in each group. The sham group received a 2.5 cm longitudinal incision in the midline part of the abdomen which was then sutured with 5-0 nylon thread;the torsion/detorsion group underwent torsion induction for 3 h followed by reperfusion for 10 days;the torsion/detorsion+O. europaea group received 300 mg/kg hydro-alcoholic extract of O. europaea 30 min before detorsion, followed by reperfusion for 10 days;and the O. europaea group only received 300 mg/kg hydro-alcoholic extract of O. europaea for 10 days. After the treatment period, blood samples were taken;the levels of estrogen, glutathione peroxidase, superoxide dismutase, and malondialdehyde were assayed. The histological changes, as well as the rate of apoptosis in ovarian tissues, were also carried out by histomorphometric analysis at day 10 post-procedure. Results: Histological comparisons demonstrated a significant detrimental change in the torsion/detorsion group as compared with other groups. The number of pre-antral and antral follicles and corpus luteum was significantly decreased in the torsion/detorsion group compared with the sham group, while treatment with O. europaea could enhance their numbers (P<0.05). The index of apoptosis and the number of atretic body in the ovarian tissue were significantly higher in the torsion/detorsion group compared with the sham group (P<0.05). The concentrations of glutathione peroxidase, estrogen, and superoxide dismutase as well as the mRNA expression of Bcl-2 were considerably diminished in the torsion/detorsion group while they were elevated in the torsion/detorsion+O. europaea group (P<0.05) compared with the torsion/detorsion group. The serum malondialdehyde level and the mRNA expression of Bax were markedly increased during ischemia, while treatment with O. europaea significantly diminished the increased concentrations of malondialdehyde and Bax level in the torsion/detorsion+O. europaea group (P<0.05). Conclusions: O. europaea extract can reduce the degree of tissue damage induced by oxidative stress and apoptosis in the ovary following ovarian ischemia/reperfusion.

Leaf morpho-physiology and phytochemistry of olive trees as affected by cultivar type and increasing aridity

The olive species(Olea europaea L.)is an ancient traditional crop grown under rainfed conditions in the Mediterranean Basin.In response to the growing national and international demand for olive oil,the olive cultivars are introduced into highly arid new bioclimatic areas.Subsequently,the morpho-physiology and phytochemistry of olive trees are potentially changing among cultivar types and geographical conditions.In the present work,we have undertaken an assessment on the impacts of geographical location and cultivar types on the leaf morpho-physiology and phytochemistry of olive trees.Thus,leaves of the two most cultivated olive tree varieties,Chemlal and Sigoise,were collected from three geographical regions(Setif,Batna,and Eloued)with increasing aridity in Algeria.Leaf samples from the geographical regions were analyzed using the standard physiological experiment,colorimetric method,and a chromatography assay.Leaves of both cultivars exhibited a significant variance in terms of the leaf shape index but not for the leaf tissue density,specific leaf weight,and specific leaf area.Photosynthetic pigment contents were affected by both cultivar type and geographical location,with the lowest pigment content recorded in the Sigoise cultivar from the Setif region.Compared with the Setif and Batna regions,dried leaves of both cultivars from the Eloued region showed the higher levels of the total polyphenol,total flavonoid,and total tannin,as well as a better antioxidant capacity.Liquid chromatography-mass spectrometry analysis of all leaf extracts identified the following phenolic acids as major compounds:oleuropein,naringin,apigenin-7-O-glucoside,kaempferol,quercetin,quercitrin,luteolin-7-O-naringenin,and quinic acid.Lower contents were found for p-Coumaric acid,trans-Ferulic acid,hyperoside,rutin,apigenin,caffeic acid,protocatechuic acid,o-Coumaric acid,and gallic acid.Also,epicatechin and catechin+were not found in the leaf extracts of the Sigoise cultivar.The leaf organic extracts in both cultivars displayed promising anti-cancer activity that was affected by geographical location and organic solvent polarity.Briefly,although increasing aridity and soil organic and mineral deficiency affected the leaf morpho-physiological parameters,both cultivars sustained a chemical richness,a good antioxidant,and an anti-tumoral capacity in leaves.Furthermore,the findings revealed that regardless the olive tree genotype,there was a significant impact of geographical location on the leaf morpho-physiology,bioactivity,and chemical composition,which may consequently modulate the growth and oil production of olive trees.

Inhibitory effects of methanolic Olea europaea and acetonic Acacia laeta on growth of Babesia and Theileria

Objective:To evaluate the antipiroplasmic activities of methanolic extract of Olea europaea(MOE)and acetonic extract of Acacia laeta(AAL)against Babesia and Theileria parasites in vitro and evaluate the chemotherapeutic effects of these extracts against Babesia(B.)microti in vivo.Methods:Fluorescence assay using SYBR Green 1 nucleic acid stain was used to detect inhibitory effects of the two extracts as well as the combination effects of the two extracts with diminazene aceturate and atovaquone on four Babesia species and Theileria equi in vitro while for in vivo experiments,8-weekold female BALB/c mice were injected intraperitoneally with 1× 107 B.microti-iRBCs and treated orally at a dose of 150 mg/kg of both extracts.Results:The half maximal inhibitory concentration(IC50)values of AAL against B.bovis,B.bigemina,B.divergens,B.caballi,and Theileria equi were lower than those of MOE extracts.Toxicity assay on Madin-Darby bovine kidney,mouse embryonic fibroblast(NIH/3T3),and human foreskin fibroblast cell lines showed that MOE and AAL affected only the viability of Madin-Darby bovine kidney cell line with half maximal effective concentrations(EC50)of(794.7±41.9)and(873.9±17.5)μg/mL,respectively.The oral treatments of MOE and AAL at 150 mg/kg inhibited the growth of B.microti in mice by 80.4% and 64.4%,respectively.The MOE and diminazene aceturate combination showed a higher chemotherapeutic effect than that of monotherapy.Conclusions:MOE and AAL have the potential to be an alternative remedy for treating piroplasmosis.Furthermore,the combination therapy of MOE + DA was more potent against B.microti infection in mice than their monotherapies.

Structural Features of the Leaf and Fruit of the Emerald Variety <i>Olea europaea</i>L., Growing under the Introduction Conditions of Surkhandarya

For the first time, the anatomical structure of the leaf, petiole and fruit of the emerald variety Olea europaea, which grows under the conditions of introduction in the Oltinsay district of the Surkhandarya region of Uzbekistan, was studied. The following characteristic diagnostic features were determined: in the leaf—the dorsiventral type of leaf mesophyll;thick-walled outer walls of the epidermis;slightly sinuous outlines of the cells of the adaxial epidermis, rectilinear-abaxial;hypostomotic leaves;non-submerged stomata of the anomocytic type;thyroid silver-gray and white-fluffy star-shaped squamous-numerous on the adaxial epidermis than on the adaxial;chlorophyll-bearing palisade and spongy parenchyma;closed collateral type of conductive bundles;the most scleralized leaf due to the presence of filamentous scleroids in it (long, thin, resembling fibers);in the petiole—parenchymal-beam type of structure;the location of the angular collenchyma under the epidermis;closed collateral type of conductive bundles;the presence of thick-walled parenchymal cells and the presence of filiformscleroids;pericarp of the fetus consists of a rigid 1-layer exocarp, parenchymal mesocarp, and sclerenchymal endocarp;the presence of scleroids—stony cells and branched sclerenchymal fibers with drops of oil in the parenchymal cells. Diagnostic signs revealed by us reflect xeromorphic of this species. All the signs were compared, and we came to the conclusion that the anatomical signs of the leaf and the fetus can be useful for providing diagnostic signs for distinguishing the studied taxa, can be used in taxonomy, and can serve to identify plant materials.

Modelling the impacts of cover crop management strategies on the water use,carbon exchange and yield of olive orchards

Cover crops have long been proposed as an alternative soil management for minimizing erosion rates in olive stands while providing additional ecosystem services.However,the trade-off between these benefits and the competition for water with the trees makes the definition of optimal management practices a challenging task in semiarid climates.This work presents an improved version of OliveCan,a process-based simulation model of olive orchards that now can simulate the main impacts of cover crops on the water and carbon balances of olive orchards.Albeit simple in its formulation,the new model components were developed to deal with different cover crop management strategies.Examples are presented for simulation runs of a traditional olive orchard in the conditions of southern Spain,evaluating the effects of different widths for the strip occupied by the cover crop(Fcc)and two contrasting mowing dates.Results revealed that high Fccresulted in lower olive yields,but only when mowing was applied at the end of spring.In this regard,late mowing and high Fccwas associated with lower soil water content from spring to summer,coinciding with olive flowering and the earlier stages of fruit growth.Fccwas also negatively correlated with surface runoff irrespective of the mowing date.On the other hand,net ecosystem productivity(NEP)was substantially affected by both Fccand mowing date.Further simulations under future climate scenarios comparing the same management alternatives are also presented,showing substantial yield reductions by the end of the century and minor or negligible changes in NEP and seasonal runoff.

Population Dynamic of Olive Pit Scale,Pollinia pollini Costa(Hemiptera:Asterolecanidae)on Two Olive Cultivars in North Region of Jordan

Olive tree,Olea europaea L.,is considered the most important tree in Jordan.The olive pit scale,Pollinia pollini Costa(Hemiptera:Asterolecanidae)has become a destructive threat to some olive groves in Jordan.A field study was conducted in an olive grove located in Northern Jordan during the growing season of 2015 to determine the population dynamics of this pest on two olive cultivars:Grossidi and Nebali Baladi,grown under rain-fed conditions.Terminal twigs were randomly sampled from the periphery of the canopy on each experimental tree at on month intervals throughout the entire year.Each sample was inspected thoroughly under a dissecting microscope in the laboratory for the presence of the immature and mature pit scale insect.The Grossidi olive cultivar was significantly more susceptible to P.pollini than Nebali Baladi,as indicated by the development of higher populations throughout the year.There was strong evidence that P.pollini overwintered as adults.Three generations were recorded per annum.The first generation occurred in February and March,followed by others from May to June and from August to November,respectively.The first two generations were significantly larger than the third one.Based on these observations,the greatest abundance of crawlers occurs in mid-March and mid-May,and therefore,application of insecticides is recommended during this period of time,since crawlers lack a waxy covering on their body and thus facilitates the chemical penetration through the cuticle,making them more susceptible to insecticides.Moreover,farmers are encouraged to cultivate the native Nebali Baladi cultivar rather than the more susceptible,introduced Grossidi cultivar in areas subject to heavy infestation.

A Comparative Experimental Research on the Traditional Cultivation Technology and Intensive Cultivation Technology of Olea europaea

In order to improve the intensive cultivation and management technology of Olea europaea in the high density orchard,through an analysis on the 3 a growth,yield and orchard construction cost for different varieties and different densities in Gansu Wudu ordinary O.europaea orchard and close O.europaea planting orchard,the appropriate conditions,varieties and density for intensive orchard construction were initially selected.The results showed that the tree height growth in the ordinary orchard was 119%of the tree height growth in the high density orchard,while the ground diameter growth in the ordinary orchard was 112%of the ground diameter growth in the high density orchard,but the yield in the ordinary orchard was only 62%of the yield in the high density orchard;of the 8 new varieties introduced from Spain,"Arbequina","Arbosana"and"Koroneiki"were the appropriate varieties for the intensive orchard construction;the ratio of the ordinary orchard construction cost to the high density orchard construction cost was 1∶6.24.Under high water and fertilizer conditions,the ratio of the ordinary orchard yield to the high density orchard yield was 1∶4.481.Therefore,in the flat land area,using the appropriate intensive cultivars,and integrating the high-tech achievements and advanced management tools,can increase output per unit area,and greatly save manpower and land costs,to achieve the purpose of high quality,high yield and stable production.In the production practice,it is necessary to vigorously promote the intensive cultivation technology of O.europaea.

The Actual Trends of Olive Growing in Albania

The olive tree （Olea Europaea） owns the status of national patrimony in Albania. In the past, olive plantations were dictated by Agricultural Policies, where olive trees were cultivated mainly in marginal lowland areas and hills. The highest efficiency in olive production is reached mainly in the Southern and Central regions of the country. After 1990s, olive farming has been highly impacted due to shifts in the economic system. Its interest in the economical aspect is going to be very important in the near future. The government has developed a national scheme for the intensification of olive farming by increasing the planted area by 60,000 ha, or in total to 20 million olive trees. Our study presents an overall view of actual trends of olive farming, olive industry, subsequent environmental problems resulting from farming and olive mills by-products, and finally the role that the government has to play in that sector,

Development and Countermeasures of Olea europaea Industry in Gansu Province

Valleys along Bailong River, Longnan City, Gansu Province are optimal growth region for olive in China, and olive industry has become a local special sup- porting industry, playing a key role in economic advancement and farmer＇s income improvement. The research analyzed development and restriction factors of olive in- dustry, and proposed some countermeasures, which are of significance for olive in- dustry development in Gansu Province.

The nutritional and therapeutic importance of Olea europaea-a review

Olea europaea(Family:Oleaceae)is the characteristic fruit tree of the Mediterranean basin.The oil extracted from the fruit was the essential product,olive oil was used for cooking and as salad oil.It was preferred for its flavor and beneficial health effects.While,low grade oil was used in cosmetics,as lubricants and for soap production.Plant parts were traditionally used in the treatment of diabetes,malaria,hypertension,coughs,asthma,lumbago,rheumatism,kidney problems,urinary tract infections,nose bleeding,for eye infections and to relieve sore throats.About 676 distinct chemical compounds were identified in the Olea europaea,they were included(fatty acids,phenolic compounds,alcohols,volatiles,phospholipids,triterpenic acids,sterols,hydrocarbons,sugars,amino acids,tocopherols,pigments,and many other compounds).Olea europaea extracts and oil possessed many pharmacological activities included cardiovascular,anti-obesity,antidiabetic,inflammatory,analgesic,antioxidant,antimicrobial,antiparasitic,anti-anticancer,immunomodulatory,respiratory,endocrine,reproductive and protective effects.This review discussed the contents,pharmacological,nutritional and therapeutic activities of Olea europaea.

Arbuscular Mycorrhizal Fungal Diversity Associated with Olea europaea L. Growing in Yunnan Province, Southwestern China

Olive(Olea europaea L.)is one of the most important and widely cultivated fruit trees,with high economic,ecological,cultural and scientific value.China began introducing and cultivating olive in the 1960s,and Yunnan Province is one of the main growing areas.Improving the cultivation and productivity of this tree crop species is an important challenge.Olive is a typical mycotrophic species and the potential of arbuscular mycorrhizal fungi(AMF)for this plant is well recognized;nevertheless,studies of olive AMF in China are still very limited.Roots and rhizosphere soils of olive were sampled from five representative growing sites in the Yunnan Province of China to investigate the AMF colonization status in the root systems,the AMF community in the olive orchards and the edaphic factors influencing the arbuscular mycorrhizal(AM)parameters.Root samples of olive trees from different growing sites generally showed AMF colonization,suggesting that autochthonous AMF manifest a high efficiency in colonizing the roots of olive plants.The spore density on the five sites ranged from 81.6 to 350 spores per 20 g soil.Twenty-three AMF species from 9 genera were identified in total,and Glomeraceae was the dominant family.The findings of our study suggested a high AMF diversity harbored by olive growing in different areas of the Yunnan Province,Southwestern China.Furthermore,the hyphal colonization in roots positively correlated with soil pH and EC.The arbuscule colonization in olive roots negatively correlated with soil pH,EC,OM,TN,TP and AN.The spore density positively correlated with OM,TN,AN,AP and sand content.Finally,the Shannon index of AMF in the rhizosphere soil positively correlated with the clay content,but negatively correlated with soil pH,TN and silt content.The high diversity of autochthonous AMF in Yunnan is promising for screening AMF isolates for utilization in the efficient cultivation of this crop.

Water Consumption and Vegetative Growth Progress in Resistant and Susceptible Olive Cultivars Infected by Verticillium dahliae

Purpose: Water consumption and vegetative growth in resistant (“Frantoio”) and susceptible (“Picual”) olive cultivars infected with Verticillium dahliae were assessed. Methods: Plants were inoculated with the pathogen and disease evaluated in growth chamber. Mean daily water consumption and vegetative growth were recorded during 13 weeks. Results: Water consumption significantly decreased in inoculated plants compared to non-inoculated plants during the 3rd - 6th weeks after inoculation period. From the 9th week, a significantly lower consumption occurred in infected “Picual” plants compared with “Frantoio” plants. Vegetative growth was reduced in inoculated plants of both cultivars, although the reduction of the fresh weight was remarkably higher in “Picual” compared with controls due to defoliation and tissue desiccation, impeding the production of new shoots and leaves. Conversely, in “Frantoio”, defoliation was significantly reduced to low levels. Conclusions: Water consumption and vegetative growth in plants of resistant (“Frantoio”) and susceptible (“Picual”) olive cultivars inoculated by Verticillium dahliae were consistently associated with the progress of the wilt symptoms and the level of resistance of the cultivar. Growth and water consumption reductions in “Picual” were irreversible in almost all plants, while “Frantoio” plants were able to maintain water translocation, overcome the infection and avoid vascular occlusion, and restart vegetative growth.

Detection of Verticillium dahliae in Olive Groves Using Canine Detection Units

Verticillium wilt is one of the most significant agricultural diseases in the world, in view of the fact that not only does it affec olive groves but also a wide variety of fruit, vegetable and ornamental plants. Currently, the most efficient and economical method of control involves the use of plant species that are resistant to the disease. However, as there are few varieties of olive tree with this characteristic, early diagnosis and the replacement of affected trees are key strategies to combat the disease. The present research proposes the use of a canine unit that is specifically trained to detect Verticillium dahliae as an early detection method for the fungus in olive groves. For the odorous samples are produced in the laboratory from V. dahliae, it is calculated that the dog dis-plays a level of sensitivity of 97% and specificity of 95%, and an initial field assay shows that the sensitivity and specificity are constant when working with infected olive trees. In view of the fact that there is currently no curative treatment for this disease and the affected plants are completely lost, fast on-site detection provides significant advantages in terms of preventing the transmission of the disease. Moreover, it also enables focalized treatment to be provided on plots of land and plants and, in combination with the latest prevention methods, real-time detection of affected olive trees enables a reduction in the costs involved in vaccination, thereby contributing overall to a reduction in the financial losses caused by verticillium wilt.

Predicting ADME/Tox properties of hydroxytyrosol in the leaves of Olea europaea L.

In order to study the possibility of hydroxytyrosol(HT)as a drug,we used SwissADME system to predict ADME of HT and pkCSM system to predict Tox of HT.The results show that hydroxytyrosol meets the Lipinski’s five principles of drug-like properties.With strong efficacy and pharmacological activity,HT has high drug-likeness degree.With good bioavailability,it can be easily absorbed by the gastrointestinal tract,though not absorbed by skin.Hydroxytyrosol has not only a strong potency and pharmacological activity,but also no liver toxicity and skin allergy.Tox data predicts that it has mutagenic potential,which may be the result of overreduction.

Effect of Regulated Deficit Irrigation on Productivity,Quality and Water Use in Olive cv“Manzanilla”

The objective of this experiment was to determine the effect of different regulated deficit irrigation (RDI) strategies on productivity, oil quality and water-use efficiency on olive grown in the Sonoran Desert. The experiments were carried out in 2009 and 2010, and in a ten years old traditional (10 × 5 m) “Manzanilla” olive orchard. The control treatment was irrigated at 100% ETc during the whole season while RDI treatments were applied at 75% ETc or 50% ETc. The two RDI were applied during two phenological stages: at postharvest to evaluate the effect on table olive or from pit hardening to harvest to evaluate the effect on oil olive. Our results indicated that RDI applying 50% ETc during postharvest period reduced significantly fruit set and table olive yield, while applied during pit hardening to harvest period, it decreased oil yield but increased oil content. The RDI applying an ETc of 75% during the postharvest period gave similar table olive yield to the control, and applied form of pit hardening to harvest also gave similar oil yield to the control. The RDI using an ETc of 75% resulted in the highest water-use efficiency for oil or table olive production.