The purpose of our study was to establish a regeneration system for micropropagation of Populus euphratica Olivier. On the basis of an analysis of plant leaf mineral nutrients, a special medium was proposed, called MP...The purpose of our study was to establish a regeneration system for micropropagation of Populus euphratica Olivier. On the basis of an analysis of plant leaf mineral nutrients, a special medium was proposed, called MP2. In optimizing media for in vitro plant cultures including MS, B5 and MP2 media we employed hormones, auxin IAA, cytokine benzyladenine （BAP） and gibberellic acid （GA） in our factorial experiments on media. Adventitious shoots were derived from cuttings of adult plants taken from Xingii- ang, west China, on selected media with MP2^＋ 0.5 mg·L^-1BA＋0.1 mg·L^-1 NAA. The shoots were elongated on a medium with 0.25 mg·L^-1 BAP, 0.1 mg·L^-1NAA and 2 mg·L^-1 GA and were then rooted on a medium with 0.2-0.5 mg·L^-1 IBA. All the media were incorporated with 30 g·L^-1 sucrose and an adjusted pH at 6.3.展开更多
文摘The purpose of our study was to establish a regeneration system for micropropagation of Populus euphratica Olivier. On the basis of an analysis of plant leaf mineral nutrients, a special medium was proposed, called MP2. In optimizing media for in vitro plant cultures including MS, B5 and MP2 media we employed hormones, auxin IAA, cytokine benzyladenine （BAP） and gibberellic acid （GA） in our factorial experiments on media. Adventitious shoots were derived from cuttings of adult plants taken from Xingii- ang, west China, on selected media with MP2^＋ 0.5 mg·L^-1BA＋0.1 mg·L^-1 NAA. The shoots were elongated on a medium with 0.25 mg·L^-1 BAP, 0.1 mg·L^-1NAA and 2 mg·L^-1 GA and were then rooted on a medium with 0.2-0.5 mg·L^-1 IBA. All the media were incorporated with 30 g·L^-1 sucrose and an adjusted pH at 6.3.
