A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

Acupuncture:Effective and Recommended but More Implementation Needed

Acupuncture enjoys a robust evidence base for dozens of clinical conditions and decades of research exploring its mechanisms of action. It has over 9,000 positive recommendations from official government and clinical guidelines. However, it still remains relatively inaccessible in the United States, Europe and elsewhere, especially compared to the strength of evidence-based recommendations for its use. Acupuncture would benefit from robust implementation strategies, utilizing insights and approaches from implementation science. The clinical use of Botox for migraine suffered from weaker evidence of effectiveness and greater evidence of harm, but using a streamlined and robust implementation strategy, Allergan was able to achieve widespread implementation from when it began its efforts around 2010. Such a systematic approach that identifies and overcomes barriers to implementation for acupuncture would benefit millions of people who currently are offered less effective and more invasive treatments, contrary to the recommendations of clinical guidelines.

Implementation Status and Development Strategies of Study Tour in Primary Schools in Nanchang City : A Case Study of L Primary School

In recent years,with the support and promotion of relevant policies such as the Opinions on Promoting Study Tour for Primary and Secondary School Students jointly issued by the Ministry of Education and 11 other departments,a wave of study tour has been sparked nationwide from individual pilot projects to gradual promotion.In this paper,students and teachers from grades 4 to 6 of L Primary School in Nanchang City,Jiangxi Province were selected as the survey objects.Combining the survey questionnaire,the current situation of conducting study tour in the primary school was sorted out and analyzed in depth from several aspects such as study tour cognition,study tour participation,study tour implementation,etc.Based on the survey results and relevant research,the current problems in conducting study tours in primary schools were summarized,and targeted improvement measures were proposed,in order to promote the healthy development of study tours in primary schools.

Role of macroscopic on-site evaluation of endoscopic ultrasoundguided fine-needle aspiration/biopsy:Results of a multicentric prospective study

BACKGROUND The concept of macroscopic on-site evaluation(MOSE)was introduced in 2015 when the endoscopist observed better diagnostic yield when the macroscopically visible core on MOSE was superior to 4 mm.Recent studies suggest that MOSE by the endoscopist may be an excellent alternative to rapid on-site evaluation,and some classi-fications have been published.Few studies have assessed the adequacy of histologic cores in MOSE during endoscopic ultrasound-guided fine-needle aspiration/biopsy(EUS-FNA/FNB).AIM To evaluate the performance of MOSE during EUS-FNA/FNB.METHODS This multicentric prospective study was conducted in 16 centers in 3 countries(Egypt,Iraq,and Morocco)and included 1108 patients with pancreatic,biliary,or gastrointestinal pathology who were referred for EUS examination.We prospectively analyzed the MOSE in 1008 patients with available histopathological reports according to 2 classifications to determine the adequacy of the histological core samples.Data management and analysis were performed using a Statistical Package for Social Sciences(SPSS)version 27.RESULTS A total of 1074 solid lesions were biopsied in 1008 patients with available cytopathological reports.Mean age was 59 years,and 509 patients(50.5%)were male.The mean lesion size was 38 mm.The most frequently utilized needles were FNB-Franseen(74.5%)and 22 G(93.4%),with a median of 2 passes.According to 2 classifications,618 non-bloody cores(61.3%)and 964 good samples(95.6%)were adequate for histological evaluation.The overall diagnostic yield of cytopathology was 95.5%.The cytological examination confirmed the diagnosis of malignancy in 861 patients(85.4%),while 45 samples(4.5%)were inconclusive.Post-procedural adverse events occurred in 33 patients(3.3%).Statistical analysis showed a difference between needle types(P=0.035)with a high sensitivity of FNB(97%).The analysis of the relationship between the MOSE-score and the final diagnosis showed a significant difference between the different scores of the MOSE(P<0.001).CONCLUSION MOSE is a simple method that allows endoscopists to increase needle passes to improve sample quality.There is significantly higher FNB sensitivity and cytopathology diagnostic yield with good MOSE cores.

A High Efficiency Hardware Implementation of S-Boxes Based on Composite Field for Advanced Encryption Standard

The SubBytes (S-box) transformation is the most crucial operation in the AES algorithm, significantly impacting the implementation performance of AES chips. To design a high-performance S-box, a segmented optimization implementation of the S-box is proposed based on the composite field inverse operation in this paper. This proposed S-box implementation is modeled using Verilog language and synthesized using Design Complier software under the premise of ensuring the correctness of the simulation result. The synthesis results show that, compared to several current S-box implementation schemes, the proposed implementation of the S-box significantly reduces the area overhead and critical path delay, then gets higher hardware efficiency. This provides strong support for realizing efficient and compact S-box ASIC designs.

Research on the Evaluation System of Community Daycare Center Planning and Implementation from the Perspective of Subject-Object Relationships

Through literature review,we found that evaluations of urban planning implementation often focus narrowly on either the subject(stakeholders)or the object(projects),while micro-level aspects such as site selection and construction are primarily considered within the context of planning implementation.There is insufficient research on the evaluation of implementation.Community daycare centers play a crucial role in the community home care model,yet there is relatively little research on their usage efficiency,satisfaction levels,and spatial evaluation of planning implementation.Based on the theoretical understanding of the human environment,including the subject,object,and their interactions,an evaluation system for the planning and implementation of community day care centers was constructed,incorporating subjective evaluation,objective evaluation,and comparative analysis.

Research on the Evaluation of the Implementation Effect of Curriculum Examination Reform: Taking the Course of “Introduction to Language” as an Example

“Introduction to Language,”as the core course of the English major,mainly reveals the basic laws of language,enabling the students to have the ability to apply linguistic theories to explain language phenomena and solve specific language problems.This course is highly theoretical and has a certain degree of difficulty.The evaluation of student grades has always been based on examination reform,but several problems have been encountered during the implementation process.This article analyzes the current situation of the reform and implementation of the“Introduction to Language”course examination in applied universities and puts forth suggestions and specific measures to improve the assessment of the course,aiming to enhance the teaching effectiveness of the course and scientifically and comprehensively evaluate students’abilities.

Research on the Implementation Path of Labor Education in Colleges and Universities from an International Perspective

Universities shoulder the important mission of talent cultivation,scientific research,social service,cultural heritage and innovation,and international exchange and cooperation.International exchange and cooperation have become the fifth major function of universities.Based on the background of the globalization era,undergraduate colleges and universities should pay more attention to the cultivation of students’intercultural communication and understanding ability while cultivating their professional skills,and take expanding students’international vision and enhancing the international competitiveness of China’s education as important tasks.Labor education is an important content of the national education system,which has the comprehensive nurturing value of cultivating morality,increasing intelligence,strengthening physical fitness,and cultivating beauty.Higher education institutions should further optimize the system of labor education,expand students’space and field of thinking in the process of labor education,and broaden students’international perspectives.

Study on Path Selection and Implementation Effect of Counselors in College Students’Career Planning Education

Under the background of a severe job market and fierce talent competition,the career planning education of college students is of great importance.In this study,a combination of quantitative and qualitative research methods was used;500 students in Shenzhen Polytechnic of Information Technology were surveyed by questionnaire,and 40 counselors were interviewed in depth to comprehensively analyze the role and practical effect of counselors in career planning education.The research showed that the role positioning of counselors includes the guide of students’career development,emotional support,and job-hunting strategy,and the specific path selection involves the strengthening of professional knowledge,the construction of interpersonal relationship networks,and personalized counseling.Based on the survey and interview data,this paper constructed a model for enhancing the role of counselors in career planning education and put forward a set of targeted work paths.

Research on the Implementation Path of Ideological and Political Education in the“Horticultural Plant Production Technology”Course under the Rural Revitalization Strategy

The horticultural industry plays an important role in the rural revitalization strategy.As the core course of horticultural technology,“Horticultural Plant Production Technology”shoulders an important mission in serving the rural revitalization strategy and cultivating a group of rural revitalization talents who truly love agriculture,know agricultural techniques,and understand agriculture.Under the dimension of ideological and political education in the curriculum,based on the strategy of rural revitalization,the implementation path of ideological and political education in the curriculum is expanded and improved from the construction of education models,curriculum team building,excavation of ideological and political elements,and construction of evaluation systems,in order to improve the level of ideological and political education in horticultural plant production technology courses and provide certain reference and guidance for the ideological and political construction of similar agricultural vocational education courses.

Construction of Ideological and Political Education in English Teacher Education Courses in Local Normal Universities:Objectives,Connotations,and Implementation Paths

The integration of ideological and political education into university curricula represents a new trend in holistic,comprehensive,and all-around education.The concept of“ideological and political education in English teacher education courses”embodies an interdisciplinary approach to curriculum reform.Grounded in the principle of fostering moral integrity,it aims to create high-quality courses by seamlessly integrating the transmission of knowledge and skills with the guidance of ideological values.This study explores the status quo,significance,and implementation pathways for integrating ideological and political education into English teacher education courses,providing valuable insights for future curricular development.

On the Models of the Bilingual Education and Their Implementation Conditions

This paper carefully probes into the main models of the bilingual education in the world, deeply analyses the conditions and requirements on the implementation of the bilingual education in our schools, and clearly differentiates TEFL and TESL as well as bilingual education. The writer thinks that in China we teach English as a foreign language instead of a second language, that the bilingual education is the supreme level of the foreign language acquisition, and that the final goal of the bilingual education is to make learners simultaneously think and use Chinese and English in both ways as well as communicate with one another in two languages according to the situational context. Therefore, it is not easy to practice the bilingual education in a country like China, which is still poor in the English language context. But the situation will change as China enters WTO and the globalization speeds up.

VLSI Implementation of a Single-Chip DVB-C Demodulator

A single-chip DVB-C quadrature amplitude modulation（QAM） demodulator is proposed,which integrates a 3.3V 10bit 40MSPS analog-to-digital converter and a forward error correction decoder. The demodulator chip can support 4-256 QAM with variable bit rate up to 80Mbps. It features a wide carrier offset acquisition range,optimal demodulation algorithm,and small circuit area. The chip is implemented in SMIC 0.25μm 1P5M mixed-signal CMOS technology with a die size of 3.5mm×3. 5mm. The maximum power consumption is 447mW.

Assessment and Implementation Intention of Circular Agriculture in Jiangsu Province with a Case of 20 Farmers' Professional Cooperatives in Huai'an City

Circular agriculture is of practical significance for Jiangsu agriculture. The research conducted quantitative analysis on circular agriculture in Jiangsu by estab- lishing assessment index system of circular agriculture development, In 2013, most indices of Jiangsu circular agriculture kept higher than national levels, but many problems exist such as excessive uses of chemical fertilizer, pesticide and mulch. Therefore, it is effective for advancing circular agriculture in Jiangsu to stabilize gov- emment fiscal support and broaden financing channels.

Product Data Management Implementation

On the basis of PDM(product data management) definition and its connotation, the factors to ensure implementation success are analyzed. The definition phase, analysis phase, design phase, build and test phase, and post production phase during PDM implementation are described. The implementation is divided into ten processes, which consist of the above different phases. The relationships between phases and processes are illustrated. Finally, a workflow is proposed to guide the implementing at a fixed price.

EFL Learner-Focused Curriculum Redesign,Implementation and Evaluation——a case from China Agricultural University

The paper investigates the learner-focused English as a Foreign Langue(EFL) curricular innovation in China Agricultural University(referred to as CAU in the following text) from the perspectives of curricular formulation,implementation and evaluation of EFL learning & teaching in the one-year duration from Autumn semester of 2016 to Spring semester of 2017.A discussion of the effects of the curricular restructuring is presented.

The Defects and Future Research Direction of the Auditof the Policy Implementation Effect 2 China

近年来,政策执行效果审计在中国逐渐引起了关注.论文首先介绍了政策执行效果审计的内涵和研究的意义,在此基础上简要阐述了政策执行效果审计的发展现状,并指出了其缺陷,最后提出了未来的研究应重点关注建立政策执行效果审计理论框架体系、完善政策执行效果审计评价指标体系、探索对政策本身的评价以及将政策执行效果审计与新兴互联网工程及信息技术结合起来等几个方面.

FAST IMPLEMENTATION OF CONVOLUTION BACKPROJECTION ALGORITHM IN SPOTLIGHT SAR

A fast implementation of the convolution backprojection（CBP）algorithm in spotlight synthetic aperture radar（SAR）is presented based on the fast Fourier transform（FFT）.Traditionally,the computation of the ＇backprojection＇ process is expensive,since resampling in the process is implemented by using the interpolation operation.By analyzing the relative location relationship among different pixels,the algorithm realizes the ＇backprojection＇ using a series of FFTs instead of the interpolation operation.The point target simulation validates that the new algorithm accelerates the CBP algorithm,and the computational rate increases about 85%.

Design and FPGA Implementation of a new hyperchaotic system

In this paper, a new four-dimensional autonomous hyperchaotic system is designed for generating complex chaotic signals. In the design, its parameters are selected according to the requirements for chaos and hyperchaos. The hyperchaotic nature is verified theoretically by using the bifurcation analysis and demonstrated experimentally by the implementation of an analogue electronic circuit. Moreover, the Field Programmable Gate Array （FPGA） technology is applied to implementing a continuous system in a digital form by using a chip of Altera Cyclone II EP2C35F484C8. The digital sequence generated from the FPGA device is observed in our experimental setup.

Findings from 2010 Global Adult Tobacco Survey: Implementation of MPOWER Policy in China

Objective To assess the implementation of five key tobacco control policies in China： protection from second-hand smoke （SHS）; offering help to quit; health warnings regarding tobacco use; the enforcement of bans on tobacco advertising, promotion, and sponsorship; and increasing tobacco taxes and prices. Methods Using 2010 Global Adults Tobacco Survey in China （GATS-China）, 10 indicators are used to assess the implementation of five key tobacco control policies of MPOWER in China. Results Overall, 63.3% and 72.7% of adultsnoticed people smoking indoor workplaces and public places, respectively. Approximately 60% of smokers were not asked about their smoking habits and approximately 67% were not advised to quit on their visit to a health worker. Sixty percent of adults noticed health warning messages on cigarette packaging and in the media in the last 30 days, 63.6% stated that they would not consider quitting. Twenty percent of respondents noticed tobacco advertising, promotion, and/or sponsorship activities in the 30 days prior to the survey. Among them, 76.3% noticed the direct advertising and 50% noticed from TV programs. Although purchasing price of one pack of cigarettes ranged from 1 to 200 RMB, 50% of current smokers （about 150 million） spent 5 RMB or less on one pack of cigarette. The expenditure on 100 packets of cigarettes represents 2% of 2009 GDP per capita. Conclusion The average score for the implementation of the 5 policies of MPOWER in China is 37.3 points, indicating tobacco control policies in China is poor and there is a large gaps from the FCTC requirements.