A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

Role of macroscopic on-site evaluation of endoscopic ultrasoundguided fine-needle aspiration/biopsy:Results of a multicentric prospective study

BACKGROUND The concept of macroscopic on-site evaluation(MOSE)was introduced in 2015 when the endoscopist observed better diagnostic yield when the macroscopically visible core on MOSE was superior to 4 mm.Recent studies suggest that MOSE by the endoscopist may be an excellent alternative to rapid on-site evaluation,and some classi-fications have been published.Few studies have assessed the adequacy of histologic cores in MOSE during endoscopic ultrasound-guided fine-needle aspiration/biopsy(EUS-FNA/FNB).AIM To evaluate the performance of MOSE during EUS-FNA/FNB.METHODS This multicentric prospective study was conducted in 16 centers in 3 countries(Egypt,Iraq,and Morocco)and included 1108 patients with pancreatic,biliary,or gastrointestinal pathology who were referred for EUS examination.We prospectively analyzed the MOSE in 1008 patients with available histopathological reports according to 2 classifications to determine the adequacy of the histological core samples.Data management and analysis were performed using a Statistical Package for Social Sciences(SPSS)version 27.RESULTS A total of 1074 solid lesions were biopsied in 1008 patients with available cytopathological reports.Mean age was 59 years,and 509 patients(50.5%)were male.The mean lesion size was 38 mm.The most frequently utilized needles were FNB-Franseen(74.5%)and 22 G(93.4%),with a median of 2 passes.According to 2 classifications,618 non-bloody cores(61.3%)and 964 good samples(95.6%)were adequate for histological evaluation.The overall diagnostic yield of cytopathology was 95.5%.The cytological examination confirmed the diagnosis of malignancy in 861 patients(85.4%),while 45 samples(4.5%)were inconclusive.Post-procedural adverse events occurred in 33 patients(3.3%).Statistical analysis showed a difference between needle types(P=0.035)with a high sensitivity of FNB(97%).The analysis of the relationship between the MOSE-score and the final diagnosis showed a significant difference between the different scores of the MOSE(P<0.001).CONCLUSION MOSE is a simple method that allows endoscopists to increase needle passes to improve sample quality.There is significantly higher FNB sensitivity and cytopathology diagnostic yield with good MOSE cores.

基于LabVIEW-VISA的传感器检测虚拟实验平台设计

为提升应用本科机电专业学生智能传感应用能力,设计传感器检测虚拟实验平台。提出基于LabVIEWVISA的传感器检测虚拟实验平台及实验方法,在LabVIEW-VISA应用设计基础上搭建了传感器检测虚拟实验平台整体架构;设计了传感器实验箱、MyDAQ便携式测量、万用表、应变式传感器等主要硬件;进行了数据采集模块、数据处理、输出模块及信号处理电路等软件设计,利用信号处理电路实现数据在平台中的双通道传输,实现了预期目标。结果表明,设计的传感器检测虚拟实验平台实验验证灵敏度高、线性误差小,有利于提高人才实践动手能力培养。

Cheesy material on macroscopic on-site evaluation after endoscopic ultrasound-guided fine-needle biopsy:Don't miss the tuberculosis

Endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)is an excellent investigation to diagnose pancreatic lesions and has shown high accuracy for its use in pathologic diagnosis.Recently,macroscopic on-site evaluation(MOSE)performed by an endoscopist was introduced as an alternative to rapid on-site cytologic evaluation to increase the diagnostic yield of EUS-FNB.The MOSE of the biopsy can estimate the adequacy of the sample directly by the macroscopic evaluation of the core tissue obtained from EUS-FNB.Isolated pancreatic tuberculosis is extremely rare and difficult to diagnose because of its non-specific signs and symptoms.Therefore,this challenging diagnosis is based on endoscopy,imaging,and the bacteriological and histological examination of tissue biopsies.This uncommon presentation of tuberculosis can be revealed as pancreatic mass mimicking cancer.EUS-FNB can be very useful in providing a valuable histopathological diagnosis.A calcified lesion with a cheesy core in MOSE must be suggestive of tuberculosis,leading to the request of the GeneXpert,which can detect Mycobacterium tuberculosis deoxyribonucleic acid and resistance to rifampicin.A decent diagnostic strategy is crucial to prevent unnecessary surgical resection and to supply conservative management with antitubercular therapy.

Rapid on-site evaluation of endoscopic-ultrasound-guided fine-needle aspiration diagnosis of pancreatic masses

Endoscopic ultrasound (EUS) has become an essential tool for the study of pancreatic diseases. Specifically, EUS plays a pivotal role evaluating patients with a known or suspected pancreatic mass. In this setting, differential diagnosis remains a clinical challenge. EUS-guided fine-needle aspiration (FNA) and fine-needle biopsy (FNB) have been proven to be safe and useful tools in this setting. EUS-guided FNA and FNB, by obtaining cytological and/or histological samples, are able to diagnose pancreatic lesions with high sensitivity and specificity. In this context, several methodological features, trying to increase the diagnostic yield of EUS-guided FNA and FNB, have been evaluated. In this review, we focus on the role of rapid on-site evaluation (ROSE). From data reported in the literature, ROSE may increase diagnostic yield of EUS-FNA specimens by 10%-30%, and thus, diagnostic accuracy. However, we should point out that many recent studies have reported adequacy rates of &#x0003e; 90% without ROSE, indicating that, perhaps, at high-volume centers, ROSE may not be indispensable to achieve excellent results. The use of ROSE can be considered important during the learning curve of EUS-FNA, and also in hospital with diagnostic accuracy rates &#x0003c; 90%.

Improved edge lightweight YOLOv4 and its application in on-site power system work

A“cloud-edge-end”collaborative system architecture is adopted for real-time security management of power system on-site work,and mobile edge computing equipment utilizes lightweight intelligent recognition algorithms for on-site risk assessment and alert.Owing to its lightweight and fast speed,YOLOv4-Tiny is often deployed on edge computing equipment for real-time video stream detection;however,its accuracy is relatively low.This study proposes an improved YOLOv4-Tiny algorithm based on attention mechanism and optimized training methods,achieving higher accuracy without compromising the speed.Specifically,a convolution block attention module branch is added to the backbone network to enhance the feature extraction capability and an efficient channel attention mechanism is added in the neck network to improve feature utilization.Moreover,three optimized training methods:transfer learning,mosaic data augmentation,and label smoothing are used to improve the training effect of this improved algorithm.Finally,an edge computing equipment experimental platform equipped with an NVIDIA Jetson Xavier NX chip is established and the newly developed algorithm is tested on it.According to the results,the speed of the improved YOLOv4-Tiny algorithm in detecting on-site dress code compliance datasets is 17.25 FPS,and the mean average precision(mAP)is increased from 70.89%to 85.03%.

Random Forests Algorithm Based Duplicate Detection in On-Site Programming Big Data Environment

On-site programming big data refers to the massive data generated in the process of software development with the characteristics of real-time,complexity and high-difficulty for processing.Therefore,data cleaning is essential for on-site programming big data.Duplicate data detection is an important step in data cleaning,which can save storage resources and enhance data consistency.Due to the insufficiency in traditional Sorted Neighborhood Method(SNM)and the difficulty of high-dimensional data detection,an optimized algorithm based on random forests with the dynamic and adaptive window size is proposed.The efficiency of the algorithm can be elevated by improving the method of the key-selection,reducing dimension of data set and using an adaptive variable size sliding window.Experimental results show that the improved SNM algorithm exhibits better performance and achieve higher accuracy.

New Explorations of Laboratory and On-site Testing of Broadband Seismographs in China

According to different testing purposes, methods and available environmental conditions, the seismograph testing can be divided into laboratory and on-site testing, respectively. The testing of the seismograph's key parameters and other concerning technical specifications are well described in guide documents(China Earthquake Administration, 2017). This includes seismometer sensitivity, linearity and clip levels based on the shake table test, as well as the seismometer natural period, damping constant based on electrical calibration(Wang Guangfu,1986; Ple?inger A.,1993) and instrumental self-noise collocation estimation(Holcomb L.G., 1989; Sleeman R. et al., 2006). However, with the development of seismic observation technology, many new requirements for the performance evaluation of seismographs have been put forward, and new testing items and methods have emerged.

Dynamical energy equipartition of the Toda model with additional on-site potentials

We study the dynamical energy equipartition properties in the integrable Toda model with additional uniform or disordered on-site energies by extensive numerical simulations. The total energy is initially equidistributed among some of the lowest frequency linear modes. For the Toda model with uniform on-site potentials, the energy spectrum keeps its profile nearly unchanged in a relatively short time scale. On a much longer time scale, the energies of tail modes increase slowly with time. Energy equipartition is far away from being attached in our studied time scale. For the Toda model with disordered on-site potentials, the energy transfers continuously to the high frequency modes and eventually towards energy equipartition. We further perform a systematic study of the equipartition time teq depending on the energy density ε and the nonlinear parameter α in the thermodynamic limit for the Toda model with disordered on-site potentials. We find teq∝ （1/ε）^a（1/α）^b, where b≈ 2a. The values of a and b are increased when increasing the strengths of disordered on-site potentials or decreasing the number of initially excited modes.

Increased Effluent Dosage Effects on On-Site Wastewater Treatment Systems of Differing Architecture Type

Approximately 20% of homes nationwide use an on-site treatment system as a form of household wastewater management. However, approximately 10% to 20% of on-site treatment systems malfunction each year, many of which have either failed or exceeded the soil’s long-term acceptance rate (LTAR), causing environmental and human health risks. The objective of this field study was to evaluate the effects of soil condition (e.g., wet and dry) and product architecture type [i.e., chamber, gravel-less-pipe (GLP), polystyrene-aggregate, and pipe-and-aggregate] on in-product solution storage and biomat thickness in a profile-limited soil in northwest Arkansas under increased loading rates and to estimate the LTAR for each product. During Phase I of this study (March 13 to October 4, 2013), effluent loading rates were approximately doubled, while rates were approximately quadrupled during Phase II (October 8, 2013 to May 29, 2014), from the maximum allowable loading rate for each product. The pipe-and-tire-chip, 46-cm-wide trench pipe-and-gravel, and the 25-cm diameter GLP products had the greatest (p < 0.001), while the 31-cm-width and the 5.4-m-long chambers had the lowest (p < 0.001) in-product solution storage during wet-soil conditions of Phase I monitoring. The 25-cm diameter GLP product had the greatest (p < 0.001), while the 61-cm-width, 5.4-m-long chamber had the lowest (p < 0.001) in-product solution storage during Phase II. Results of this study indicate that some alternative products may be able to effectively handle effluent loading rates in excess of those currently allowed by the State of Arkansas. Further research will be required to confirm these interpretations.

A rapid on-site calibration method for measuring dynamic envelope curve of high-speed trains

Dynamic envelope curve is a significant parameter to assess the running safety of high-speed trains.Up to now the method based on binocular stereo vision is the only way available to measure the dynamic envelope curve of a train,the speed of which is over200km/h.Nevertheless the method has two limitations,one is large field-〇f-view(FO V),the other is calibration time.Hence portable calibration equipment,easy-t〇-build target and rapid calibration algorithm are required to complete the calibration.In this paper,a new rapid on-site calibration method with large FOV based on binocular stereo vision is proposed.To address these issues,a light target has been designed,the rail coordinate system(RCS)is represented by40fixed retroreflective points on the target,and they are utilized to calibrate the parameters of two cameras.In addition,two cameras merely capture a single image of the target simultaneously,and the intrinsic and extrinsic parameters of the cameras can be calculated rapidly.To testify the proposed method,the experiments have been conducted and the results reveal that the accuracy can reach+1mm,which can meet the measurement requirement.

On-Site Calibration Method of Dosimeter Based on X-Ray Source

The real-time monitoring of environmental radiation dose for nuclear fa-cilities is an important part of safety, in order to guarantee the accuracy of the monitoring results regular calibration is necessary. Around nuclear facilities there are so many environmental dosimeters installed dispers-edly, because of its huge quantity, widely distributed, and in real-time monitoring state;it will cost lots of manpower and finance if it were tak-en to calibrate on standard laboratory;what’s more it will make the en-vironment out of control. To solve the problem of the measurement ac-curacy of the stationary gamma radiation dosimeter, an on-site calibra-tion method is proposed. The radioactive source is X-ray spectrum, and the dose reference instrument which has been calibrated by the national standard laboratory is a high pressure ionization. On-site calibration is divided into two parts;firstly the energy response experiment of dosim-eter for high and low energy is done in the laboratory, and the energy response curve is obtained combining with Monte Carlo simulation;sec-ondly experiment is carried out in the field of the measuring dosimeter, and the substitution method to calibrate the dosimeter is used;finally the calibration coefficient is gotten through energy curve correction. In order to verify the accuracy of on-site calibration method, the calibrated dosimeter is test in the standard laboratory and the error is 3.4%. The re-sult shows that the on-site calibration method using X-ray is feasible, and it can improves the accuracy of the measurement results of the stationary &gamma;-ray instrument;what’s more important is that it has great reference value for the radiation safety management and radiation environment evaluation.

An Experience of On-site PD Testing for Condition Monitoring of an 11 kV PILC Cable Insulation System

A cable circuit of a substation in the United Kingdom showed high level of PD activities during a survey using hand hold PD testing equipment. The authors were invited to carry out on-site PD testing experiment to further diagnose and locate the potential problem of the cable system. This paper presents the experience of the present authors carrying out the cable test. Following a brief introduction to the experiment equipments and physical connections, the paper analyses the data collected from the testing, including PD pulse shape analysis, frequency spectrum analysis and phase resolved PD pattern analysis. Associated with PD propagation direction identification, PD source diagnosis and localisation was made. Four different types of sensors, which were adapted during the testing, are shown to have different frequency bandwidths and performed differently. Aider comparing the parameters of the sensor and the PD signals detected by individual sensor, optimal PD monitoring bandwidth for cable system is suggested.

基于LabVIEW VISA智能车无线调试系统

使用低成本的无线传输模块并结合LabVIEW可以快速开发成本低廉无线数据调试系统,为各种移动设备的研发提供实用的工具。介绍一种低成本车用无线调试系统,主要说明了基于XS128单片机硬件系统的设计和基于LabVIEW VISA的虚拟仪器设计。实现了通过单个串口进行多路数据的采集、无线传输及上位机显示,并具备下位机参数修改功能。对系统的稳定性进行了一定的测试,给出了稳定性和数据传输率的测试结果。

基于VISA技术的自动测试软件研制

介绍了一种用于核测量的自动测试软件的开发。该软件通过使用VISA技术和面向对象技术,实现了在一台PC机上利用多种仪器控制接口同时控制多台型号不同的数据记录设备;并且通过网络编程技术,实现了基于LAN的实验数据传输、备份与显示。同时对软件主要功能的实现方法作出介绍。

USB-GPIB控制器及VISA函数库的设计

本文将USB2.0接口应用于GPIB控制器的设计与开发,介绍了USB—GPIB控制器的硬件实现和VISA函数库软件设计中的技术难点及解决方案,包括:通信协议、资源管理、格式化输入/输出和资源互斥访问等问题,并提出了基于此控制器的自动测试系统组建方案。目前,采用本文所提出的设计方案开发的USB—GPIB控制器和相关VISA函数库已经产品化,其主要性能指标已达到或优于国内外同类产品。

虚拟仪器软件结构VISA的研究

VISA是VXI即插即用系统联盟制定的标准化I／O软件，是实现VXI自动测试系统标准化的关键。讨论了VISA的应用与实现中的主要问题。

LXI标准下的网络VISA设计与实现

LXI仪器的软件驱动器以网络VISA作为其I/O接口以适应LXI总线标准的要求,可见网络VISA的设计势在必行;在研究了LXI标准和仪器驱动器设计方法的基础上,提出将VISA函数编制成32位动态链接库提供给上层函数或应用程序调用的设计方法;具体实现则结合TCP/IP网络传输协议,通过VXI-11网络发现机制和远程过程调用完成网络控制端与仪器的寻址和I/O通信;最后给出了LXI标准下的网络VISA几个主要函数的设计流程图和具体程序,程序代码通过在Microsoft Visual C++环境下建立应用工程调用动态连接库测试连接、程控LAN设备成功证明可行。

基于VISA的事件处理方法与实现

通过对虚拟仪器系统I/O接口软件———VISA特点及其资源描述的分析 ,着重比较分析了几种基于VISA的事件处理方法 ,并结合实例 ,给出了各种方法的实现 ,在虚拟仪器系统软件设计中有广泛的运用。

利用LabWindows/CVI的VISA体系对仪器进行I/O的操作

介绍了虚拟仪器的基本概念和VISA体系的主要特点,并举例说明利用VISA提供的函数库对仪器进行I/O操作的过程,并在此基础上提出了一种新的虚拟仪器测试系统设计方法。