A novel immunosensor for determination of clenbuterol was described in this paper. Horseradish peroxidase (HRP) and Monoclonal antibody against clenbuterol was covalently immobilized onto the cellulose acetate membran...A novel immunosensor for determination of clenbuterol was described in this paper. Horseradish peroxidase (HRP) and Monoclonal antibody against clenbuterol was covalently immobilized onto the cellulose acetate membrane which was used as one-touch immunochips. After 20 min of competitive reaction of clenbuterol in samples against glucose oxidase-conjugated clenbuterol (CLB-GOD) with anti-clenbuteol antibody immobilized on the membrane, the one-touch transducer was fixed on the Nafion - ferrocene modified glass-carbon electrode (GCE, working electrode) by “O” ring. The increased current changes (ΔI) were recorded for the quantitative determination of clenbuterol by derivative cyclic voltammetry. Experimental results showed that a very effective system for electron transmission had been established using dual-enzyme system and Nafion-ferrocene modified GCE. Quantitative analysis of clenbuterol could be easily performed on this kind of immunosensor, which showed the advantage of time-saving and high sensitivity (The detecting limit of this kind of immunosensor can be down to 100 ng/L). In addition, other conditions affecting the electrochemical response were also discussed in detail.展开更多
FRANCISCO Little enjoys a stable lifestyle in Beijing. Every workday morning, he rides Mobike, one of the shared bike brands, from his apartment to the subway station and takes the subway to his place of work, At noon...FRANCISCO Little enjoys a stable lifestyle in Beijing. Every workday morning, he rides Mobike, one of the shared bike brands, from his apartment to the subway station and takes the subway to his place of work, At noon, he orders lunch in the office or goes to a nearby Starbucks for coffee. After work, he buys fresh vegetables at a local market before heading home. On weekends, he either travels to other cities or meets up with friends to try out new restaurants, All of these activities are done without needing any cash, just via a touch on his cellphone.展开更多
文摘A novel immunosensor for determination of clenbuterol was described in this paper. Horseradish peroxidase (HRP) and Monoclonal antibody against clenbuterol was covalently immobilized onto the cellulose acetate membrane which was used as one-touch immunochips. After 20 min of competitive reaction of clenbuterol in samples against glucose oxidase-conjugated clenbuterol (CLB-GOD) with anti-clenbuteol antibody immobilized on the membrane, the one-touch transducer was fixed on the Nafion - ferrocene modified glass-carbon electrode (GCE, working electrode) by “O” ring. The increased current changes (ΔI) were recorded for the quantitative determination of clenbuterol by derivative cyclic voltammetry. Experimental results showed that a very effective system for electron transmission had been established using dual-enzyme system and Nafion-ferrocene modified GCE. Quantitative analysis of clenbuterol could be easily performed on this kind of immunosensor, which showed the advantage of time-saving and high sensitivity (The detecting limit of this kind of immunosensor can be down to 100 ng/L). In addition, other conditions affecting the electrochemical response were also discussed in detail.
文摘FRANCISCO Little enjoys a stable lifestyle in Beijing. Every workday morning, he rides Mobike, one of the shared bike brands, from his apartment to the subway station and takes the subway to his place of work, At noon, he orders lunch in the office or goes to a nearby Starbucks for coffee. After work, he buys fresh vegetables at a local market before heading home. On weekends, he either travels to other cities or meets up with friends to try out new restaurants, All of these activities are done without needing any cash, just via a touch on his cellphone.
