With the clinical development and application of intracytoplasmic sperm injection(ICSI)technology in human assisted reproduction,the influence of oocyte quality on embryo development has been paid more and more attent...With the clinical development and application of intracytoplasmic sperm injection(ICSI)technology in human assisted reproduction,the influence of oocyte quality on embryo development has been paid more and more attention.So far,there have been many reports on oocyte morphology affecting embryo development.It has been found in some works that the appearance of smooth endoplasmic reticulum clusters(SERC)in oocytes may affect the fertilization and embryo development of oocytes.However,with the increasing reports of SERC-containing oocytes obtained by in vitro fertilization and healthy offspring in recent years,there is still some controversy on whether to continue to use SERC-containing oocytes for the following assisted reproductive therapy in clinical practice.Based on this,this review aims to review the research progress of SERC in oocytes in recent years.展开更多
objective:The management of oocytes affected by smooth endoplasmic reticulum aggregates(SERa)remains debatable.To understand how to manage SERa+oocytes and cycles,we performed a retrospective cohort study and analyzed...objective:The management of oocytes affected by smooth endoplasmic reticulum aggregates(SERa)remains debatable.To understand how to manage SERa+oocytes and cycles,we performed a retrospective cohort study and analyzed the impact of SERa+cycles and oocytes on clinical and neonatal outcomes.Methods:We included 4856 cycles(149 SERa+and 4707 SERa-)from 4201 women(age:21-42 years)who received in vitro fertilization/intracytoplasmic sperm injection(IVF/ICSl)treatments at the Center of Reproductive Medicine,First Affliated Hospital of Army Military University,from 2016 to 2019.SERa+cycles had at least one SERa oocyte in the oocyte cohort.All 1722 oocytes in the SERa+cycle were divided into SERa+(405)and SERa-(1317)oocytes.Results:The rates of two pronuclei(2PN)and high-quality embryos were lower in SERa+cycles than in SERa-cycles,regardless of IVF or ICSI(P<0.05).As the proportion of SERa+oocytes increased in the SERa+cycles,the rate of high-quality embryos declined gradually(P<0.05).Furthermore,the rate of 2PN in SERa+oocytes was significantly lower than that in SERa-oocytes(P<0.05).Regardless of whether IVF or ICSl insemination was performed,no significant differences in terms of clinical pregnancy rate and spontaneous abortion rate were observed between SERa+and SERa-cycles or between SERa+and SERa-oocytes(P>0.05).conclusion:Normal fertilization with SERa+cycles and oocytes was substantially reduced,regardless of the insemination method.Embryos originating from SERa+oocytes can be transferred when there are no other options,but fully informed consent and strict follow-up of fetal development are mandatory.展开更多
目的:研究卵胞浆内精子注射(ICSI)周期卵母细胞滑面内质网(sER)聚集对早期胚胎体外发育及妊娠结局的影响并分析其原因。方法:99例患者ICSI治疗107个周期,于HCG日测定血清激素E2、P、LH浓度以及子宫内膜厚度。根据所获MⅡ期卵母细胞是否...目的:研究卵胞浆内精子注射(ICSI)周期卵母细胞滑面内质网(sER)聚集对早期胚胎体外发育及妊娠结局的影响并分析其原因。方法:99例患者ICSI治疗107个周期,于HCG日测定血清激素E2、P、LH浓度以及子宫内膜厚度。根据所获MⅡ期卵母细胞是否出现sER聚集分为A、B两组,A组为所获MⅡ期卵母细胞均未出现sER聚集周期共90例;B组为有至少一枚MⅡ期卵母细胞出现sER聚集周期,共17例。比较A、B两组HCG日E2、P、LH水平、子宫内膜厚度,及治疗周期数、年龄、病程、Gn总量、Gn天数、获卵数、受精率、可用胚胎率、优质胚胎率、移植胚胎数、周期临床妊娠率、种植率、流产率和原发、继发不孕患者比例的差异。并且对比B组sER聚集阳性[sER(+)]与sER聚集阴性[sER(-)]卵母细胞的受精率、可用胚胎率和优质胚胎率。结果:B组HCG日E2水平明显高于A组(P<0.05,3141.18±604.47 vs 2635.12±825.46),而两组间P和LH水平以及子宫内膜厚度均无显著差异(P>0.05)。B组优质胚胎率显著低于A组(P<0.05,47.83%vs 57.67%),而B组Gn天数(P<0.01,13.35±1.66 vs 11.83±2.4)和流产率(P<0.05,100%vs 17.86%)均明显高于A组,两组在年龄、治疗周期数、病程、Gn总量、获卵数、受精率、可用胚胎率、移植胚胎数、临床妊娠率、种植率以及原发、继发不孕患者比例等各方面均无显著差异(P>0.05)。B组sER(+)卵母细胞的优质胚胎率显著低于sER-卵母细胞(P<0.01,20.69%vs 52.9%),而受精率和可用胚胎率无显著性差异(P>0.05)。结论:ICSI周期卵母细胞sER聚集对早期胚胎体外发育及妊娠结局均有不良影响,sER聚集可能与HCG日高E2水平及长时间Gn刺激有关。展开更多
人类辅助生殖技术过程中的促排卵药物会导致卵母细胞胞浆出现多种形态异常。卵母细胞滑面内质网聚集(aggregates of smooth endoplasmic reticulum,SERa)就是胞浆异常的一种,能够影响胚胎质量和临床结局。临床上针对SERa的卵母细胞的处...人类辅助生殖技术过程中的促排卵药物会导致卵母细胞胞浆出现多种形态异常。卵母细胞滑面内质网聚集(aggregates of smooth endoplasmic reticulum,SERa)就是胞浆异常的一种,能够影响胚胎质量和临床结局。临床上针对SERa的卵母细胞的处理方法不统一。有健康新生儿出生报道认为丢弃SERa的卵母细胞是一种不谨慎的表现,特别是对于可用卵子数量有限的夫妇,可能增加移植周期取消率,导致受孕机会减低。对于SERa的形成原因,生殖工作者应该继续深入研究其机制,寻找避免SERa形成的干预策略,改善不孕症患者的临床结局。展开更多
基金National Natural Science Foundation of China (No.82072880,81960283)Science and Technology Project of Hainan Province (No.LCYX202102)Key Science and Technology Project of Hainan Province (No.ZDKJ2017007)。
文摘With the clinical development and application of intracytoplasmic sperm injection(ICSI)technology in human assisted reproduction,the influence of oocyte quality on embryo development has been paid more and more attention.So far,there have been many reports on oocyte morphology affecting embryo development.It has been found in some works that the appearance of smooth endoplasmic reticulum clusters(SERC)in oocytes may affect the fertilization and embryo development of oocytes.However,with the increasing reports of SERC-containing oocytes obtained by in vitro fertilization and healthy offspring in recent years,there is still some controversy on whether to continue to use SERC-containing oocytes for the following assisted reproductive therapy in clinical practice.Based on this,this review aims to review the research progress of SERC in oocytes in recent years.
基金supported by the Prevention and Control Research Project of Reproductive Health and Major Birth Defects(2017YFC1002001)Study on the Safety of Reproductive and Assisted Reproductive Technology in Aged Women(cstc2017shms-zdyx0035).
文摘objective:The management of oocytes affected by smooth endoplasmic reticulum aggregates(SERa)remains debatable.To understand how to manage SERa+oocytes and cycles,we performed a retrospective cohort study and analyzed the impact of SERa+cycles and oocytes on clinical and neonatal outcomes.Methods:We included 4856 cycles(149 SERa+and 4707 SERa-)from 4201 women(age:21-42 years)who received in vitro fertilization/intracytoplasmic sperm injection(IVF/ICSl)treatments at the Center of Reproductive Medicine,First Affliated Hospital of Army Military University,from 2016 to 2019.SERa+cycles had at least one SERa oocyte in the oocyte cohort.All 1722 oocytes in the SERa+cycle were divided into SERa+(405)and SERa-(1317)oocytes.Results:The rates of two pronuclei(2PN)and high-quality embryos were lower in SERa+cycles than in SERa-cycles,regardless of IVF or ICSI(P<0.05).As the proportion of SERa+oocytes increased in the SERa+cycles,the rate of high-quality embryos declined gradually(P<0.05).Furthermore,the rate of 2PN in SERa+oocytes was significantly lower than that in SERa-oocytes(P<0.05).Regardless of whether IVF or ICSl insemination was performed,no significant differences in terms of clinical pregnancy rate and spontaneous abortion rate were observed between SERa+and SERa-cycles or between SERa+and SERa-oocytes(P>0.05).conclusion:Normal fertilization with SERa+cycles and oocytes was substantially reduced,regardless of the insemination method.Embryos originating from SERa+oocytes can be transferred when there are no other options,but fully informed consent and strict follow-up of fetal development are mandatory.
文摘目的:研究卵胞浆内精子注射(ICSI)周期卵母细胞滑面内质网(sER)聚集对早期胚胎体外发育及妊娠结局的影响并分析其原因。方法:99例患者ICSI治疗107个周期,于HCG日测定血清激素E2、P、LH浓度以及子宫内膜厚度。根据所获MⅡ期卵母细胞是否出现sER聚集分为A、B两组,A组为所获MⅡ期卵母细胞均未出现sER聚集周期共90例;B组为有至少一枚MⅡ期卵母细胞出现sER聚集周期,共17例。比较A、B两组HCG日E2、P、LH水平、子宫内膜厚度,及治疗周期数、年龄、病程、Gn总量、Gn天数、获卵数、受精率、可用胚胎率、优质胚胎率、移植胚胎数、周期临床妊娠率、种植率、流产率和原发、继发不孕患者比例的差异。并且对比B组sER聚集阳性[sER(+)]与sER聚集阴性[sER(-)]卵母细胞的受精率、可用胚胎率和优质胚胎率。结果:B组HCG日E2水平明显高于A组(P<0.05,3141.18±604.47 vs 2635.12±825.46),而两组间P和LH水平以及子宫内膜厚度均无显著差异(P>0.05)。B组优质胚胎率显著低于A组(P<0.05,47.83%vs 57.67%),而B组Gn天数(P<0.01,13.35±1.66 vs 11.83±2.4)和流产率(P<0.05,100%vs 17.86%)均明显高于A组,两组在年龄、治疗周期数、病程、Gn总量、获卵数、受精率、可用胚胎率、移植胚胎数、临床妊娠率、种植率以及原发、继发不孕患者比例等各方面均无显著差异(P>0.05)。B组sER(+)卵母细胞的优质胚胎率显著低于sER-卵母细胞(P<0.01,20.69%vs 52.9%),而受精率和可用胚胎率无显著性差异(P>0.05)。结论:ICSI周期卵母细胞sER聚集对早期胚胎体外发育及妊娠结局均有不良影响,sER聚集可能与HCG日高E2水平及长时间Gn刺激有关。
文摘人类辅助生殖技术过程中的促排卵药物会导致卵母细胞胞浆出现多种形态异常。卵母细胞滑面内质网聚集(aggregates of smooth endoplasmic reticulum,SERa)就是胞浆异常的一种,能够影响胚胎质量和临床结局。临床上针对SERa的卵母细胞的处理方法不统一。有健康新生儿出生报道认为丢弃SERa的卵母细胞是一种不谨慎的表现,特别是对于可用卵子数量有限的夫妇,可能增加移植周期取消率,导致受孕机会减低。对于SERa的形成原因,生殖工作者应该继续深入研究其机制,寻找避免SERa形成的干预策略,改善不孕症患者的临床结局。
