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铁皮石斛疫病及其病原菌 被引量:34
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作者 李静 张敬泽 +2 位作者 吴晓鹏 单卫星 徐同 《菌物学报》 CAS CSCD 北大核心 2008年第2期171-176,共6页
铁皮石斛疫病2001年发现在浙江义乌栽培田间,是由疫霉菌引起的。在田间,疫霉菌侵染茎基部,引起当年移植苗根腐、植株枯萎和死亡,但侵染2-3年植株幼嫩顶部仅引起顶枯症状。通过对病原菌形态学、交配型的观察,以及核糖体DNAITS序列分析,... 铁皮石斛疫病2001年发现在浙江义乌栽培田间,是由疫霉菌引起的。在田间,疫霉菌侵染茎基部,引起当年移植苗根腐、植株枯萎和死亡,但侵染2-3年植株幼嫩顶部仅引起顶枯症状。通过对病原菌形态学、交配型的观察,以及核糖体DNAITS序列分析,侵染铁皮石斛的5个分离菌株被鉴定为烟草疫霉菌Phytophthora nicotianae。致病性试验表明,铁皮石斛是烟草疫霉菌的寄主。 展开更多
关键词 卵菌 鉴定 烟草疫霉菌 铁皮石斛 疫病
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辽宁省农田作物根围的真菌(I) 被引量:11
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作者 梁晨 吕国忠 《沈阳农业大学学报》 CAS CSCD 2002年第3期185-187,共3页
报道了分离自辽宁省12市县农田作物根围真菌中的7属9种 :小克银汉霉属(Cunninghamella)1种 ,班尼小克银汉霉(CunninghamellabainieriNaumov) ;毛霉属(Mucor)1种 ,冻土毛霉冻土变种(MucorhiemalisWehmerf.hiemalis) ;毛壳菌属(Chaetomiu... 报道了分离自辽宁省12市县农田作物根围真菌中的7属9种 :小克银汉霉属(Cunninghamella)1种 ,班尼小克银汉霉(CunninghamellabainieriNaumov) ;毛霉属(Mucor)1种 ,冻土毛霉冻土变种(MucorhiemalisWehmerf.hiemalis) ;毛壳菌属(Chaetomium)2种:球毛壳(ChaetomiumglobosumKunze &Steud.) ;橄榄色毛壳(ChaetomiumolivaceumCooke &Ellis) ;光黑壳属(Preussia)2种:竞争光黑壳(Preussiaaemulans(Rehm)V.Arx) ,香蒲光黑壳(PreussiatypharumCain) ;假裸囊菌属(Pscudogymnoascus)1种,玫红假裸囊菌(PseudogymnoascusroseusRaillo) ;篮状菌属(Talaromyces)1种,黄色篮状菌(Talaromycesflavus(Klcker)Stolk &Samson) ;韦斯特壳属(Westerdykella)1种:散布韦斯特壳(Westerdykelladispersa(Clum)Cejp &Milko) ;其中班尼小克银汉霉、竞争光黑壳、香蒲光黑壳。 展开更多
关键词 辽宁 农田作物 根围 真菌 土壤
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茶多酚对不同植物病原菌抑制作用的研究 被引量:5
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作者 刘增辉 孟云 徐燕 《化学与生物工程》 CAS 2019年第3期22-26,共5页
茶多酚具有抑菌作用,但是对植物病原菌,尤其是对植物病原真菌的抑制作用目前还未有报道。以从绿茶中萃取的茶多酚粗品为供试药剂,通过室内菌丝生长速率法考察了茶多酚粗品对多种植物病原菌的抑制作用,并研究了温度对茶多酚抑菌活性的影... 茶多酚具有抑菌作用,但是对植物病原菌,尤其是对植物病原真菌的抑制作用目前还未有报道。以从绿茶中萃取的茶多酚粗品为供试药剂,通过室内菌丝生长速率法考察了茶多酚粗品对多种植物病原菌的抑制作用,并研究了温度对茶多酚抑菌活性的影响。结果表明,茶多酚对卵菌门病原菌的抑制效果较好(IC_(50)值为0.000 7~0.003 g·mL^(-1)),其次为炭疽菌属病原菌(IC_(50)值为0.003~0.05 g·mL^(-1)),而对水稻纹枯病菌、小麦纹枯病菌、小麦赤霉病菌和玉米弯孢病菌无明显抑制作用。同时,高浓度茶多酚对短小芽孢杆菌的抑制作用显著。当培养基温度为65℃时加入茶多酚,茶多酚的抑菌活性发生显著变化,对小麦纹枯病菌的抑制作用显著。因此,茶多酚可作为植物病原菌抑菌剂。 展开更多
关键词 茶多酚 植物病原菌 卵菌门 炭疽菌属 抑制作用
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腐霉与真菌(英文)
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作者 余永年 《江西科学》 2001年第1期55-60,共6页
系根据作者 2 0 0 0年 7月应韩国农业振兴厅国家农业科学技术研究院的邀请 ,在韩国讲学时的一篇手稿整理而成。全文共 3部分 :第 1部分简述 2 0世纪下半叶菌物学的三大成就。第 2部分讨论卵菌的特征及其有争议的分类地位。第
关键词 真菌 卵菌 腐霉 食用菌
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Diversity of non-Laboulbenialean fungi on millipedes
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作者 Enghoff H Reboleira ASPS De-Gang Zhao 《Studies in Fungi》 2017年第1期130-137,共8页
New records are given for secondary capilliconidia of Basidiobolus from several species of millipedes(Diplopoda)belonging to three different orders.The anamorph‘Thaxteriola‟stage of Rhynchomyces is recorded(with doub... New records are given for secondary capilliconidia of Basidiobolus from several species of millipedes(Diplopoda)belonging to three different orders.The anamorph‘Thaxteriola‟stage of Rhynchomyces is recorded(with doubt)from a Brazilian millipede belonging to the order Spirostreptida.An enigmatic fungus showing characteristics of Coreomycetopsis,Hormiscioideus and Antennopsis is recorded from two species of Danish millipedes of the order Julida.Peculiar structures,tentatively referred to fungi are recorded from several millipede orders where they occur between micro-scutes of the external cuticle. 展开更多
关键词 Amphoromorpha Antennopsis ASCOMYCOTA Basidiobolus Coreomycetopsis Diplopoda Hormiscioideus Mucoromycotina Pyxidiophora Rhynchomyces Zygomycota
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Fungal diversity notes 1611-1716: taxonomic and phylogenetic contributions on fungal genera and species emphasis in south China
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作者 Indunil C.Senanayake Walter Rossi +104 位作者 Marco Leonardi Alex Weir Mark McHugh Kunhiraman C.Rajeshkumar Rajnish K.Verma Samantha C.Karunarathna Saowaluck Tibpromma Nikhil Ashtekar Sreejith K.Ashtamoorthy Sanjay Raveendran Gurmeet Kour Aishwarya Singh Saúl De la Peña-Lastra Antonio Mateos Miroslav Kolařík Vladimír Antonín HanaŠevčíková Fernando Esteve-Raventós Ellen Larsson Fermín Pancorbo Gabriel Moreno Alberto Altés Yolanda Turégano Tian-Ye Du Li Lu Qi-Rui Li Ji-Chuan Kang Sugantha Gunaseelan Kezhocuyi Kezo Malarvizhi Kaliyaperumal Jizhen Fu Milan C.Samarakoon Yusufjon Gafforov Shakhnoza Teshaboeva Pradeep C.Kunjan Arya Chamaparambath Adam Flakus Javier Etayo Pamela Rodriguez-Flakus Mikhail P.Zhurbenko Nimali Ide Silva Danushka S.Tennakoon KPDeepna Latha Patinjareveettil Manimohan KNAnil Raj Mark S.Calabon Abdollah Ahmadpour Zeinab Heidarian Zahra Alavi Fatemeh Alavi Youbert Ghosta Razmig Azizi Mei Luo Min-Ping Zhao Nuwan D.Kularathnage Li Hua Yun-Hui Yang Chun-Fang Liao Hai-Jun Zhao Anis S.Lestari Subashini C.Jayasiri Feng-Ming Yu Lei Lei Jian-Wei Liu Omid Karimi Song-Ming Tang Ya-Ru Sun Yong Wang Ming Zeng Zin H.Htet Benedetto T.Linaldeddu Artur Alves Alan J.L.Phillips Carlo Bregant Lucio Montecchio AndréDe Kesel Vincent P.Hustad Andrew N.Miller Anna G.Fedosova Viktor Kučera Mubashar Raza Muzammil Hussain Yan-Peng Chen Vinodhini Thiyagaraja Deecksha Gomdola Achala R.Rathnayaka Asha J.Dissanayake Nakarin Suwannarach Sinang Hongsanan Sajeewa S.N.Maharachchikumbura Lakmali S.Dissanayake Nalin N.Wijayawardene Rungtiwa Phookamsak Saisamorn Lumyong E.B.Gareth Jones Neelamanie Yapa Dhanushka N.Wanasinghe Ning Xie Mingkwan Doilom Ishara S.Manawasinghe Jian-Kui(Jack)Liu Qi Zhao Biao Xu Kevin D.Hyde Jiage Song 《Fungal Diversity》 SCIE 2023年第5期161-403,共243页
This article is the 15th contribution in the Fungal Diversity Notes series,wherein 115 taxa from three phyla,nine classes,28 orders,48 families,and 64 genera are treated.Fungal taxa described and illustrated in the pr... This article is the 15th contribution in the Fungal Diversity Notes series,wherein 115 taxa from three phyla,nine classes,28 orders,48 families,and 64 genera are treated.Fungal taxa described and illustrated in the present study include a new family,five new genera,61 new species,five new combinations,one synonym,one new variety and 31 records on new hosts or new geographical distributions.Ageratinicolaceae fam.nov.is introduced and accommodated in Pleosporales.The new genera introduced in this study are Ageratinicola,Kevinia,Pseudomultiseptospora(Parabambusicolaceae),Marasmiellomycena,and Vizzinia(Porotheleaceae).Newly described species are Abrothallus altoandinus,Ageratinicola kunmingensis,Allocryptovalsa aceris,Allophoma yuccae,Apiospora cannae,A.elliptica,A.pallidesporae,Boeremia wisteriae,Calycina papaeana,Clypeo-coccum lichenostigmoides,Coniochaeta riskali-shoyakubovii,Cryphonectria kunmingensis,Diaporthe angustiapiculata,D.campylandrae,D.longipapillata,Diatrypella guangdongense,Dothiorella franceschinii,Endocalyx phoenicis,Epicoc-cum terminosporum,Fulvifomes karaiensis,F.pannaensis,Ganoderma ghatensis,Hysterobrevium baoshanense,Inocybe avellaneorosea,I.lucida,Jahnula oblonga,Kevinia lignicola,Kirschsteiniothelia guangdongensis,Laboulbenia caprina,L.clavulata,L.cobiae,L.cosmodisci,L.nilotica,L.omalii,L.robusta,L.similis,L.stigmatophora,Laccaria rubriporus,Lasiodiplodia morindae,Lyophyllum agnijum,Marasmiellomycena pseudoomphaliiformis,Melomastia beihaiensis,Nemania guangdongensis,Nigrograna thailandica,Nigrospora ficuum,Oxydothis chinensis,O.yunnanensis,Petriella thailandica,Phaeoacremonium chinensis,Phialocephala chinensis,Phytophthora debattistii,Polyplosphaeria nigrospora,Pronectria loweniae,Seriascoma acutispora,Setoseptoria bambusae,Stictis anomianthi,Tarzetta tibetensis,Tarzetta urceolata,Tetraploa obpyriformis,Trichoglossum beninense,and Tricoderma pyrrosiae.We provide an emendation for Urnula ailaoshanensis Agaricus duplocingulatoides var.brevisporus introduced as a new variety based on morphology and phylogeny. 展开更多
关键词 AGARICOMYCETES Ascomycota BASIDIOMYCOTA DOTHIDEOMYCETES Eurotiomycetes Fungal diversity Geoglossomycetes Geographical distribution Host records Incertae sedis Laboulbeniomycetes LECANOROMYCETES Leotiomycetes Novel taxa New combination OOMYCOTA Peronosporea Pezizomycetes Phylogeny SORDARIOMYCETES South Chinese fungi Taxonomy
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Fungal diversity notes 253-366:taxonomic and phylogenetic contributions to fungal taxa 被引量:6
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作者 Guo Jie Li Kevin D.Hyde +138 位作者 Rui Lin Zhao Sinang Hongsanan Faten Awad Abdel-Aziz Mohamed A.Abdel-Wahab Pablo Alvarado Genivaldo Alves-Silva Joseph F.Ammirati Hiran A.Ariyawansa Abhishek Baghela Ali Hassan Bahkali Michael Beug D.Jayarama Bhat Dimitar Bojantchev Thitiya Boonpratuang Timur S.Bulgakov Erio Camporesi Marcela CBoro Oldriska Ceska Dyutiparna Chakraborty Jia Jia Chen K.W.Thilini Chethana Putarak Chomnunti Giovanni Consiglio Bao Kai Cui Dong Qin Dai Yu Cheng Dai Dinushani A.Daranagama Kanad Das Monika C.Dayarathne Eske De Crop Rafael J.V.De Oliveira Carlos Alberto Fragoso de Souza JoséIde Souza Bryn T.M.Dentinger Asha J.Dissanayake Mingkwan Doilom E.Ricardo Drechsler-Santos Masoomeh Ghobad-Nejhad Sean P.Gilmore Aristóteles Góes-Neto MichałGorczak Charles H.Haitjema Kalani Kanchana Hapuarachchi Akira Hashimoto Mao Qiang He John K.Henske Kazuyuki Hirayama Maria J.Iribarren Subashini C.Jayasiri Ruvishika S.Jayawardena Sun Jeong Jeon Gustavo H.Jerônimo Ana L.Jesus E.B.Gareth Jones Ji Chuan Kang Samantha C.Karunarathna Paul M.Kirk Sirinapa Konta Eric Kuhnert Ewald Langer Haeng Sub Lee Hyang Burm Lee Wen Jing Li Xing Hong Li Kare Liimatainen Diogo Xavier Lima Chuan Gen Lin Jian Kui Liu Xings Zhong Liu Zuo Yi Liu J.Jennifer Luangsa-ard Robert Lücking H.Thorsten Lumbsch Saisamorn Lumyong Eduardo M.Leaño Agostina V.Marano Misato Matsumura Eric H.C.McKenzie Suchada Mongkolsamrit Peter E.Mortimer Thi Thuong Thuong Nguyen Tuula Niskanen Chada Norphanphoun Michelle A.O’Malley Sittiporn Parnmen Julia Pawłowska Rekhani H.Perera Rungtiwa Phookamsak Chayanard Phukhamsakda Carmen L.A.Pires-Zottarelli Olivier Raspé Mateus A.Reck Sarah C.O.Rocha AndréL.C.M.Ade Santiago Indunil C.Senanayake Ledo Setti Qiu Ju Shang Sanjay K.Singh Esteban B.Sir Kevin V.Solomon Jie Song Prasert Srikitikulchai Marc Stadler Satinee Suetrong Hayato Takahashi Takumasa Takahashi Kazuaki Tanaka Li Ping Tang Kasun M.Thambugala Donnaya Thanakitpipattana Michael K.Theodorou Benjarong Thongbai Tuksaporn Thummarukcharoen Qing Tian Saowaluck Tibpromma Annemieke Verbeken Alfredo Vizzini Josef Vlasák Kerstin Voigt Dhanushka N.Wanasinghe Yong Wang Gothamie Weerakoon Hua An Wen Ting Chi Wen Nalin N.Wijayawardene Sarunyou Wongkanoun Marta Wrzosek Yuan Pin Xiao Jian Chu Xu Ji Ye Yan Jing Yang Shu Da Yang Yu Hu Jin Feng Zhang Jie Zhao Li Wei Zhou Derek Peršoh Alan J.L.Phillips Sajeewa S.N.Maharachchikumbura 《Fungal Diversity》 SCIE 2016年第3期1-237,共237页
Notes on 113 fungal taxa are compiled in this paper,including 11 new genera,89 new species,one new subspecies,three new combinations and seven reference specimens.Awide geographic and taxonomic range of fungal taxa ar... Notes on 113 fungal taxa are compiled in this paper,including 11 new genera,89 new species,one new subspecies,three new combinations and seven reference specimens.Awide geographic and taxonomic range of fungal taxa are detailed.In the Ascomycota the new genera Angustospora(Testudinaceae),Camporesia(Xylariaceae),Clematidis,Crassiparies(Pleosporales genera incertae sedis),Farasanispora,Longiostiolum(Pleosporales genera incertae sedis),Multilocularia(Parabambusicolaceae),Neophaeocryptopus(Dothideaceae),Parameliola(Pleosporales genera incertae sedis),and Towyspora(Lentitheciaceae)are introduced.Newly introduced species are Angustospora nilensis,Aniptodera aquibella,Annulohypoxylon albidiscum,Astrocystis thailandica,Camporesia sambuci,Clematidis italica,Colletotrichum menispermi,C.quinquefoliae,Comoclathris pimpinellae,Crassiparies quadrisporus,Cytospora salicicola,Diatrype thailandica,Dothiorella rhamni,Durotheca macrostroma,Farasanispora avicenniae,Halorosellinia rhizophorae,Humicola koreana,Hypoxylon lilloi,Kirschsteiniothelia tectonae,Lindgomyces okinawaensis,Longiostiolum tectonae,Lophiostoma pseudoarmatisporum,Moelleriella phukhiaoensis,M.pongdueatensis,Mucoharknessia anthoxanthi,Multilocularia bambusae,Multiseptospora thysanolaenae,Neophaeocryptopus cytisi,Ocellularia arachchigei,O.ratnapurensis,Ochronectria thailandica,Ophiocordyceps karstii,Parameliola acaciae,P.dimocarpi,Parastagonospora cumpignensis,Pseudodidymosphaeria phlei,Polyplosphaeria thailandica,Pseudolachnella brevifusiformis,Psiloglonium macrosporum,Rhabdodiscus albodenticulatus,Rosellinia chiangmaiensis,Saccothecium rubi,Seimatosporium pseudocornii,S.pseudorosae,Sigarispora ononidis and Towyspora aestuari.New combinations are provided for Eutiarosporella dactylidis(sexual morph described and illus trated)and Pseudocamarosporium pini.Descriptions,illustrations and/or reference specimens are designated for Aposphaeria corallinolutea,Cryptovalsa ampelina,Dothiorella vidmadera,Ophiocordyceps formosana,Petrakia echinata,Phragmoporthe conformis and Pseudocamarosporium pini.The new species of Basidiomycota are Agaricus coccyginus,A.luteofibrillosus,Amanita atrobrunnea,A.digitosa,A.gleocystidiosa,A.pyriformis,A.strobilipes,Bondarzewia tibetica,Cortinarius albosericeus,C.badioflavidus,C.dentigratus,C.duboisensis,C.fragrantissimus,C.roseobasilis,C.vinaceobrunneus,C.vinaceogrisescens,C.wahkiacus,Cyanoboletus hymenoglutinosus,Fomitiporia atlantica,F.subtilissima,Ganoderma wuzhishanensis,Inonotus shoreicola,Lactifluus armeniacus,L.ramipilosus,Leccinum indoaurantiacum,Musumecia alpina,M.sardoa,Russula amethystina subp.tengii and R.wangii are introduced.Descriptions,illustrations,notes and/or reference specimens are designated for Clarkeinda trachodes,Dentocorticium ussuricum,Galzinia longibasidia,Lentinus stuppeus and Leptocorticium tenellum.The other new genera,species new combinations are Anaeromyces robustus,Neocallimastix californiae and Piromyces finnis from Neocallimastigomycota,Phytophthora estuarina,P.rhizophorae,Salispina,S.intermedia,S.lobata and S.spinosa from Oomycota,and Absidia stercoraria,Gongronella orasabula,Mortierella calciphila,Mucor caatinguensis,M.koreanus,M.merdicola and Rhizopus koreanus in Zygomycota. 展开更多
关键词 ASCOMYCOTA BASIDIOMYCOTA Neocallimastigomycota oomycota.zygomycota Phylogeny Taxonomy New genus New species
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菌物基因组测定研究进展 被引量:5
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作者 刘淑艳 李广 李玉 《吉林农业大学学报》 CAS CSCD 北大核心 2014年第1期1-9,16,共10页
菌物基因组因其相对较小、基因结构较简单、容易被测序和注释、细胞周期短、易于遗传操作等优点而成为真核生物基因组研究的最佳模式生物。文中综述了菌物基因组的结构特点,整理了菌物基因组测序相关信息,并简单描述了重要模式菌物的基... 菌物基因组因其相对较小、基因结构较简单、容易被测序和注释、细胞周期短、易于遗传操作等优点而成为真核生物基因组研究的最佳模式生物。文中综述了菌物基因组的结构特点,整理了菌物基因组测序相关信息,并简单描述了重要模式菌物的基因组概况,为进一步开展菌物基因组研究提供重要数据信息。 展开更多
关键词 基因组 黏菌 子囊菌 担子菌 卵菌
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中国腐霉新记录种Pythium heterothallicum的分离鉴定及致病性测定 被引量:8
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作者 甘辉林 柴兆祥 +1 位作者 楼兵干 李金花 《菌物学报》 CAS CSCD 北大核心 2010年第4期494-501,共8页
2007年9-10月,在甘肃兰州、张掖等地采集玉米、豇豆、葡萄、白菜等作物收获后的耕作层土壤,采用花瓣诱导和组织分离相结合的方法分离土壤腐霉菌。对腐霉菌株经菌丝先端切割法纯化培养后,以形态和培养特性为基础进行了初步鉴定,发现有7... 2007年9-10月,在甘肃兰州、张掖等地采集玉米、豇豆、葡萄、白菜等作物收获后的耕作层土壤,采用花瓣诱导和组织分离相结合的方法分离土壤腐霉菌。对腐霉菌株经菌丝先端切割法纯化培养后,以形态和培养特性为基础进行了初步鉴定,发现有7株腐霉菌的形态学和培养特性一致。以其中的P3-1A为代表菌株,结合rDNA-ITS序列和β-tubulin基因序列测定和比对,将这7个菌株鉴定为Pythium heterothallicum,该菌为中国新记录,异宗配合种。通过土埋法,对玉米、番茄、黄瓜等3种作物进行了致病性测定,结果表明该腐霉菌对玉米具有致病性,可引起根腐、根尖变褐、茎基腐等症状,抑制植株生长,使植株表现不同程度的矮化,但对番茄、黄瓜未见有明显为害。 展开更多
关键词 卵菌 rDNA-ITS序列 β微管蛋白基因序列 致病性
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大豆疫霉菌的EMS化学诱变 被引量:2
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作者 哈霞 胡中慧 +2 位作者 王蕾 权军利 单卫星 《菌物学报》 CAS CSCD 北大核心 2010年第2期222-227,共6页
以甲基磺酸乙酯(ethylmethane sulfonate,EMS)为诱变剂,通过其对大豆疫霉菌Phytophthora sojae休止孢萌发的影响,确定化学诱变条件。通过收集单卵孢子,建立了包含640个单卵孢子系的突变体库,其中约有50%的诱变菌系在培养性状和菌落形态... 以甲基磺酸乙酯(ethylmethane sulfonate,EMS)为诱变剂,通过其对大豆疫霉菌Phytophthora sojae休止孢萌发的影响,确定化学诱变条件。通过收集单卵孢子,建立了包含640个单卵孢子系的突变体库,其中约有50%的诱变菌系在培养性状和菌落形态方面发生了明显变化,菌落形态多样,表现出较紧密或松散,近圆形或不规则;气生菌丝减少,生长速度较慢或快;在卵孢子产量方面,8.13%的菌系有增加,20.41%的菌系减少,27.82%的菌系极少或者没有卵孢子产生,43.64%的菌系卵孢子产量类似野生型。以质膜氢离子泵蛋白基因PsPMA1(plasma membrane H+-ATPase1)为对象,通过TILLING技术,从320个大豆疫霉菌突变体中获得9个突变体,进一步确认了EMS对大豆疫霉菌的诱变效果,并且估算EMS对大豆疫霉菌的诱变频率至多每115kb发生一个核苷酸变异。新构建的突变体库为开展大豆疫霉病菌的功能基因组研究奠定了遗传材料基础。 展开更多
关键词 卵菌 卵孢子 化学诱变 突变体库
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西藏接合菌物种多样性初探 被引量:3
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作者 李政宏 普布次仁 +2 位作者 吕美林 旺姆 刘小勇 《微生物学通报》 CAS CSCD 北大核心 2018年第6期1250-1261,共12页
【背景】接合菌在自然界广泛分布,在工业、食品、医药、生物防治等方面扮演着重要的角色,部分接合菌为有害菌。西藏地区接合菌只有少数零星的报道,其系统调查研究几乎还是空白,大量潜在的物种需要分离、鉴定、认识、保藏和开发。【目的... 【背景】接合菌在自然界广泛分布,在工业、食品、医药、生物防治等方面扮演着重要的角色,部分接合菌为有害菌。西藏地区接合菌只有少数零星的报道,其系统调查研究几乎还是空白,大量潜在的物种需要分离、鉴定、认识、保藏和开发。【目的】了解西藏地区的接合菌物种多样性和生物资源现状,为该地区有害接合菌的控制和有益接合菌的开发利用奠定基础。【方法】对西藏全境7个地区19个代表县的701个样品采用平板稀释分离法得到单菌落,然后进行形态和分子(SSU、ITS和LSU r DNA)鉴定,并在此基础上进行生物多样性以及优势和稀有属种分析。【结果】得到西藏接合菌10属26种,包括5个西藏已知种和21个西藏新记录种;其中4个为中国新记录种,分别是:类变形被孢霉(Mortierella amoeboidea)、球孢高山被孢霉(M.globalpina)、灰褐毛霉(Mucor brunneogriseus)、暗色毛霉(M.fuscus)。西藏接合菌的多样性指数分析表明,不同地区间的物种数量和组成存在显著差异,排在前4位的分别是波密县、米林县、当雄县和八宿县。属种分析显示,西藏接合菌的2个优势属为被孢霉属(Mortierella)和毛霉属(Mucor);3个优势种为高山被孢霉(Mortierella alpina)、冻土毛霉(Mucor hiemalis)、匍枝根霉(Rhizopus stolonifer);常见属8个,分别是犁头霉属(Absidia)、放射毛霉属(Actinomucor)、小克银汉霉属(Cunninghamella)、根毛霉属(Rhizomucor)、根霉属(Rhizopus)、共头霉属(Syncephalastrum)、伞形霉属(Umbelopsis)和接霉属(Zygorhynchus);常见种9个,稀有种14个。【结论】西藏地区接合菌资源丰富,各地区生物多样性差异显著,稀有物种占一半以上的比例提示西藏自然环境保护的重要性。 展开更多
关键词 接合菌门 新记录 生物多样性 遗传多样性 西藏
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基于高通量测序分析连栀矾溶液发酵炮制过程中真菌菌群多样性变化 被引量:10
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作者 袁学刚 叶羊 +5 位作者 赵甲元 谢洁 金艳 肖宇航 王战国 张晓喻 《中草药》 CAS CSCD 北大核心 2018年第18期4259-4268,共10页
目的探究传统连栀矾溶液发酵炮制过程中真菌菌群多样性和优势菌群,为科学发酵提供理论依据。方法采用Illumina HiSeq高通量测序技术测定不同发酵时间上下层连栀矾溶液中真菌ITS2区序列,应用QIIME、Mothur和R等软件整理和统计样品序列数... 目的探究传统连栀矾溶液发酵炮制过程中真菌菌群多样性和优势菌群,为科学发酵提供理论依据。方法采用Illumina HiSeq高通量测序技术测定不同发酵时间上下层连栀矾溶液中真菌ITS2区序列,应用QIIME、Mothur和R等软件整理和统计样品序列数目和操作分类单元(OTU)数量,分析样品中真菌菌群的组成、丰度、分布、alpha多样性、beta多样性,阐明炮制过程中真菌菌群结构、多样性及丰富度的动态变化。结果 4个不同的发酵时期共获得用于分析的有效序列数为207 068,稀疏曲线表明测序深度充分,OTUs的数量接近于饱和,能充分展示连栀矾溶液的真菌群落结构,其主要由子囊菌门(Ascomycota,74.21%)、担子菌门(Basidiomycota,7.31%)、罗兹菌门(Rozellomycota,2.62%)、接合菌门(Zygomycota,0.66%)和球囊菌门(Glomeromycota,0.01%)组成,优势菌门为子囊菌门。通过分析OTUs数量、chao1指数,ACE指数、Shannon指数,表明连栀矾溶液真菌菌群有较高的丰富度和多样性,并且在炮制过程中上下层总丰富度和多样性随发酵时间的增加逐渐增加,其中上层样品的丰富度、多样性逐渐增加,下层样品的丰富度、多样性先降低后增加,且上层样品的丰富度、多样性明显高于下层样品。结论以高通量测序技术分析连栀矾溶液发酵炮制过程中真菌菌群结构、多样性及丰富度的动态变化可为指导其科学发酵提供参考。 展开更多
关键词 连栀矾溶液 真菌 菌群多样性 ILLUMINA HiSeq高通量测序技术 发酵 炮制 优势菌群 ITS2 菌群丰富度 子囊菌门 担子菌门 罗兹菌门 接合菌门 球囊菌门
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One stop shop:backbones trees for important phytopathogenic genera:Ⅰ(2014) 被引量:7
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作者 Kevin D.Hyde R.Henrik Nilsson +37 位作者 S.Aisyah Alias Hiran A.Ariyawansa Jaime E.Blair Lei Cai Arthur W.A.M.de Cock Asha J.Dissanayake Sally L.Glockling Ishani D.Goonasekara Michal Gorczak Matthias Hahn Ruvishika S.Jayawardena Jan A.L.van Kan Matthew H.Laurence C.AndréLévesque Xinghong Li Jian-Kui Liu Sajeewa S.N.Maharachchikumbura Dimuthu S.Manamgoda Frank N.Martin Eric H.C.McKenzie Alistair R.McTaggart Peter E.Mortimer Prakash V.R.Nair Julia Pawlowska Tara L.Rintoul Roger G.Shivas Christoffel F.J.Spies Brett A.Summerell Paul W.J.Taylor Razak B.Terhem Dhanushka Udayanga Niloofar Vaghefi Grit Walther Mateusz Wilk Marta Wrzosek Jian-Chu Xu JiYe Yan Nan Zhou 《Fungal Diversity》 SCIE 2014年第4期21-125,共105页
Many fungi are pathogenic on plants and cause significant damage in agriculture and forestry.They are also part of the natural ecosystem and may play a role in regulating plant numbers/density.Morphological identifica... Many fungi are pathogenic on plants and cause significant damage in agriculture and forestry.They are also part of the natural ecosystem and may play a role in regulating plant numbers/density.Morphological identification and analysis of plant pathogenic fungi,while important,is often hampered by the scarcity of discriminatory taxonomic characters and the endophytic or inconspicuous nature of these fungi.Molecular(DNA sequence)data for plant pathogenic fungi have emerged as key information for diagnostic and classification studies,although hampered in part by non-standard laboratory practices and analytical methods.To facilitate current and future research,this study provides phylogenetic synopses for 25 groups of plant pathogenic fungi in the Ascomycota,Basidiomycota,Mucormycotina(Fungi),and Oomycota,using recent molecular data,up-to-date names,and the latest taxonomic insights.Lineagespecific laboratory protocols together with advice on their application,as well as general observations,are also provided.We hope to maintain updated backbone trees of these fungal lineages over time and to publish them jointly as new data emerge.Researchers of plant pathogenic fungi not covered by the present study are invited to join this future effort.Bipolaris,Botryosphaeriaceae,Botryosphaeria,Botrytis,Choanephora,Colletotrichum,Curvularia,Diaporthe,Diplodia,Dothiorella,Fusarium,Gilbertella,Lasiodiplodia,Mucor,Neofusicoccum,Pestalotiopsis,Phyllosticta,Phytophthora,Puccinia,Pyrenophora,Pythium,Rhizopus,Stagonosporopsis,Ustilago and Verticillium are dealt with in this paper. 展开更多
关键词 ASCOMYCOTA BASIDIOMYCOTA ENDOPHYTES Mucormycotina Molecular identification OOMYCOTA Plant pathogens PROTOZOA
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