Optimization of ISSR-PCR Reaction System and Preliminary Construction of ISSR Fingerprinting of Some Species in Bryaceae

[Objective] The aim was to provide molecular basis for the identification of species in the moss family Bryaceae by the construction of inter-simple sequence repeats （ISSR） fingerprinting. [Method] In order to seek standardizing PCR reaction set-up, an orthogonal design was used to optimize ISSR-PCR amplification system of Bryaceae in five factors （Mg2＋, dNTPs, primer, DNA template, Taq DNA polymerase） at four levels respectively. [Result] A suitable ISSR reaction system was obtained, namely： 20 μl reaction system containing 5 ng of DNA template, 0.2 μmol/L primer, 2.25 mmol/L MgCl2, 0.6 U of Taq DNA polymerase, 0.4 mmol/L dNTPs. Proper annealing temperature was found at 48-50 ℃.The above system and six ISSR-PCR primers were used for the PCR amplification of 14 samples from Bryaceae and the related species in Mniaceae. A total of 86 bands were amplified, all showed polymorphism. NJ cluster analysis showed a star-shaped cladogram. [Conclusion] The results manifested that ISSR fingerprinting could provide the appropriate degree of polymorphism at low taxonomic level, so it would be a useful tool to provide additional evidence for resolving taxonomic relationships at the species level of Bryaceae.

Relationships among hamstring muscle optimal length and hamstring flexibility and strength

Background:Hamstring muscle strain injury(hamstring injury) due to excessive muscle strain is one of the most common injuries in sports.The relationships among hamstring muscle optimal lengths and hamstring flexibility and strength were unknown,which limited our understanding of risk factors for hamstring injury.This study was aimed at examining the relationships among hamstring muscle optimal length and flexibility and strength.Methods:Hamstring flexibility and isokinetic strength data and three-dimensional kinematic data for hamstring isokinetic tests were collected for11 male and 10 female recreational athletes.The maximal hamstring muscle forces,optimal lengths,and muscle lengths in standing were determined for each participant.Results:Hamstring muscle optimal lengths were significantly correlated to hamstring flexibility score and gender,but not to hamstring strength.The greater the flexibility score,the longer the hamstring muscle optimal length.With the same flexibility score,females tend to have shorter hamstring optimal muscle lengths compared to males.Hamstring flexibility score and hamstring strength were not correlated.Hamstring muscle optimal lengths were longer than but not significantly correlated to corresponding hamstring muscle lengths in standing.Conclusion:Hamstring flexibility may affect hamstring muscle maximum strain in movements.With similar hamstring flexibility,hamstring muscle maximal strain in a given movement may be different between genders.Hamstring muscle lengths in standing should not be used as an approximation of their optimal lengths in calculation of hamstring muscle strain in musculoskeletal system modeling.

Optimization of evidence-based research in the prevention and treatment of coronary heart disease with traditional Chinese medicine:A comprehensive review

Coronary heart disease(CHD) has severely impacted the lives and health of patients for ages. Although the modern methods used for the prevention and treatment of CHD have been increasingly perfected,clinical problems remain that require solutions. In the prevention and treatment of CHD with traditional Chinese medicine(TCM), great therapeutic advantages have been demonstrated. However, the expression of its advantages relies on scientific evidence-based research and evaluation, which require further optimization and improvement. Therefore, this review aims to comprehensively emphasize our understanding of the current deficiencies in TCM evidence-based studies and the necessity of adopting scientific evidence-based optimization methods for use in research. In this process, evidence should be produced in a standard manner, and the advantages of TCM patterns should be accurately stated because these factors contribute to efficient transformation and proper verification of the obtained evidence.Additionally, focusing on patient-oriented medicine and doctor-patient relationships is also essential.These methods are of great significance to prove the clinical efficacy of TCM and promote its development.

Evaluation on Radar Reflectivity-Rainfall Rate (Z-R) Relationships for Guyana

The constant development of science and technology in weather radar results in high-resolution spatial and temporal rainfall estimates and improved early warnings of meteorological phenomena such as flood [1]. Weather radars do not measure the rainfall amount directly, so a relationship between the reflectivity (Z) and rainfall rate (R), called the Z-R relationship (Z = aRb), where a and b are empirical constants, can be used to estimate the rainfall amount. In this research, mathematical techniques were used to find the best climatological Z-R relationships for the Low Coastal Plain of Guyana. The reflectivity data from the S-Band Doppler Weather Radar for February 17 and 21, 2011 and May 8, 2012 together with the daily rainfall depths at 29 rainfall stations located within a 150 km radius were investigated. A climatological Z-R relationship type Z = 200R1.6 (Marshall-Palmer) configured by default into the radar system was used to investigate the correlation between the radar reflectivity and the rainfall by gauges. The same data sets were used with two distinct experimental Z-R relationships, Z = 300R1.4 (WSR-88D Convective) and Z = 250R1.2 (Rosenfeld Tropical) to determine if any could be applicable for area of study. By comprehensive regression analysis, New Z-R and R-Z relationships for each of the three events aforementioned were developed. In addition, a combination of all the samples for all three events were used to produce another relationship called “All in One”. Statistical measures were then applied to detect BIAS and Error STD in order to produce more evidence-based results. It is proven that different Z-R relationships could be calibrated into the radar system to provide more accurate rainfall estimation.

Understanding the Variability of Z-R Relationships Caused by Natural Variations in Raindrop Size Distributions(DSD):Implication of Drop Size and Number

In the issue of rainfall estimation by radar through the necessary relationship between radar reflectivity Z and rain rate R (Z-R), the main limitation is attributed to the variability of this relationship. Indeed, several pre-vious studies have shown the great variability of this relationship in space and time, from a rainfall event to another and even within a single rainfall event. Recent studies have shown that the variability of raindrop size distributions and thereby Z-R relationships is therefore, more the result of complex dynamic, thermody-namic and microphysical processes within rainfall systems than a convective/stratiform classification of the ground rainfall signature. The raindrop number and size at ground being the resultant of various processes mentioned above, a suitable approach would be to analyze their variability in relation to that of Z-R relation-ship. In this study, we investigated the total raindrop concentration number NT and the median volume di-ameter D0 used in numerous studies, and have shown that the combination of these two ‘observed’ parame-ters appears to be an interesting approach to better understand the variability of the Z-R relationships in the rainfall events, without assuming a certain analytical raindrop size distribution model (exponential, gamma, or log-normal). The present study is based on the analysis of disdrometer data collected at different seasons and places in Africa, and aims to show the degree of the raindrop size and number implication in regard to the Z-R relationships variability.

Research on the Optimal Vegetation Cover for Remote Sensing Assessment of Soil Erosion Risk Using the Temporal Matching Relationship between Rainfall and Vegetation

Vegetation cover derived from remote sensing image is widely used for soil erosion risk assessment, but there is no clear guideline to select the most appropriate temporal satellite data. It is common practice that satellite data during growing season are randomly selected and used in soil erosion risk assessment. However, the effectiveness of vegetation in protecting the soil is quite different even if it is the same growing season since vegetation covers change as they grow. This article aims to provide a method of choosing optimal vegetation cover for studying soil erosion risk using remote sensing, that is, the vegetation cover in the most appropriate temporal period. Based on the temporal relationship of the two most active impact factors, rainfall and vegetation, an index of RV is developed and used to indicate the relative erosion risk during the year. The results show that annual variation of rainfall is significant, and vegetation is relatively stable, resulting in their matching relationship is different in each year. The correlation coefficient reaches 0.89 between RV and real sediment transport during the period when rainfall can cause soil erosion. In other words, RV is a good indicator of soil erosion. Therefore, there is a good correlation between RV maximum and the optimal vegetation cover, which can help facilitate erosion research in the future, showing good potential for successful application in other places.

“City-Scene” Characteristics and Optimization Strategies Research on Three Gorges Reservoir Area of the Post-Three Gorges Era

In the long-term symbiotic evolution process,the town areas along the Yangtze River and its natural and cultural landscapes have presented a sound "city-scene" landscape form. But with the implementation of the Three Gorges project,the town relocation as well as the flooding in the natural and human landscape area,the original harmonious "city-scene" pattern started to collapse and fell into the situation of "fragmentation" and "islanding". The differentiation between city and landscape,uncoordinated scale and fractured urban context become pressing issues that affect the integral form optimization of the town areas. This paper aims at responding to the call of the government about proceeding the construction of the Yangtze River economic belt under the ecological and green strategy and seeking an attuned "cityscene" relationship of the town area. Based on the analysis of the dynamic evolution of the "city-scene" relationship,the paper also pointed out that the urban construction around the reservoir area in the postThree Gorges era was the key stage of "scene-city" integration and symbiosis while the maintenance of scenery pattern,the harmony of space scale and the protection of living culture should be applied as the vital strategies.

基于最优化算法的北京市新一代天气雷达Z-R关系研究

运用最优化方法,选取北京市新一代天气雷达2007年4场降雨的基数据资料与对应的雨量计资料进行Z-R关系研究,对不同类型的降雨和不同距离范围的雷达估测点分别优化。结果表明:运用优化后的Z-R关系估测降水量的误差要比缺省的Z-R关系式直接计算出降水量的误差小得多;层状云降水的估测结果优于对流云降水的估测;距离雷达站较近的地区其估测结果优于较远的地区。

雷达估测对流性降水的误差空间分布及Z-R关系的优化

新一代天气雷达联合雨量站数据进行定量降水估测中,雷达回波强度和雨量站观测值较好的匹配是准确拟合出Z-R关系的关键。对流性降水回波强度具有时空变化大的特点,严重影响了雷达和雨量站数据的匹配以及定量降水估测的精度。利用河南地区3次强对流天气过程的雷达和雨量站数据,通过分析雷达对流性降水与地面雨量站观测值的误差空间分布,找到如下规律:雷达回波强度严重高估点和低估点大部分位于强回波的边缘地区(单点回波强度随时间变化较大);部分位于强回波中心的雨量站观测值为零(部分时刻)。通过分析可知,突发的强降水和强回波中心容易造成雨量站观测值误差较大,因此对Z-R关系拟合造成了较大的影响。根据对流性降水回波强度变化特点和本文得到的回波强度与雨量站雨量匹配关系,在Z-R关系拟合中,提出了加入回波强度随时间变化的指标对误差较大雨量站进行剔除的方法,也提出了一种优化的回波强度平均方法和累积时间,得到了用于对流性降水估测的最佳方案。详细分析以上因素对降水估测的影响表明,通过根据回波强度随时间变化的指标对雨量站进行剔除和使用最佳降水估测方案,提高了对流性降水估测的精度。

Mass transfer mechanism and relationship of gas–liquid annular flow in a microfluidic cross-junction device

Mass transfer performance of gas–liquid two-phase flow at microscale is the basis of application of microreactor in gas–liquid reaction systems.At present,few researches on the mass transfer property of annular flow have been reported.Therefore,the mass transfer mechanism and relationship of gas–liquid annular flow in a microfluidic cross-junction device are studied in the present study.We find that the main factors,i.e.,flow pattern,liquid film thickness,liquid hydraulic retention time,phase interface fluctuation,and gas flow vorticity,which influence the flow mass transfer property,are directly affected both by gas and liquid flow velocities.But the influences of gas and liquid velocities on different mass transfer influencing factors are different.Thereout,the fitting relationships between gas and liquid flow velocities and mass transfer influencing factors are established.By comparing the results from calculations using fitting equations and simulations,it shows that the fitting equations have relatively high degrees of accuracy.Finally,the Pareto front,namely the Pareto optimal solution set,of gas and liquid velocity conditions for the best flow mass transfer property is obtained using the method of multi-objective particle swarm optimization.It is proved that the mass transfer property of the gas–liquid two-phase flow can be obviously enhanced under the guidance of the obtained Pareto optimal solution set through experimental verification.

Water-cut rising mechanism and optimized water injection technology for deepwater turbidite sandstone oilfield: A case study of AKPO Oilfield in Niger Delta Basin, West Africa

Through the analysis of the reservoir connection relationship and the water-cut rising rules after water breakthrough in the highly volatile oil AKPO oilfield, a new model of water-cut rising was established, and the timing and strategy of water injection were put forward. The water-cut rising shapes of producers after water breakthrough can be divided into three types, and their water-cut rising mechanism is mainly controlled by reservoir connectivity. For the producers which directly connect with injectors in the single-phase sand body of the single-phase channel or lobe with good reservoir connectivity, the water-cut rising curve is "sub-convex". For the producers which connect with injectors through sand bodies developed in multi-phases with good inner sand connectivity but poorer physical property and connectivity at the overlapping parts of sands, the response to water injection is slow and the water-cut rising curve is "sub-concave". For the producers which connect with injectors through multi-phase sand bodies with reservoir physical properties, connectivity in between the former two and characteristics of both direct connection and overlapping connection, the response to water injection is slightly slower and the water-cut rising curve is "sub-S". Based on ratio relationship of oil and water relative permeability, a new model of water cut rising was established. Through the fitting analysis of actual production data, the optimal timing and corresponding technology for water injection after water breakthrough were put forward. Composite channel and lobe reservoirs can adopt water injection strategies concentrating on improving the vertical sweep efficiency and areal sweep efficiency respectively. This technology has worked well in the AKPO oilfield and can guide the development of similar oilfields.

Consistency in <i>Z</i>-<i>R</i>Relationship Variability Regardless Precipitating Systems, Climatic Zones Observed from Two Types of Disdrometer

Data from rain Drop Size Distributions gathered on five sites in Africa as well as those of the pilot site in Kourou (French Guyana, South America), located in different climatic zones, and collected by two types of disdrometer (the impact JW RD-69 disdrometer and the Optical Spectro-Pluviometer, OSP) are used to study the consistency of the reflectivity factor-rain rate at the ground (Z-R) relationship variability. The results clearly confirm that the relationship Z-R knows a large spatial variability, from a type of precipitation to another and within the same precipitation regardless the type of disdrometer used for DSD measurements. Base on the similarity of the relations reflectivity factor-rain rate and ratio median volume diameter over the total number of drops-rain rate, the variability of the Z-R coefficients (A, b) through the simultaneously implication of the size and number of drops which characterize the DSD was exhibited. It was shown that the relationships A-α and b-β designed to understand the involvement of parameters D0 and NT of DSD in the variability of the relationship Z-R are similar regardless the types of disdrometer used. However, the relations A-α in the Sahelian region appear to deviate from those of Guinean, equatorial and Soudanian zones. The plausible reasons were discussed.

优化Z-R关系及其在北京地区面雨量估测中的应用

运用最优化方法,选取北京市新一代天气雷达2007年5场降雨的基数据资料与对应的地面雨量计资料进行Z-R关系研究,对比由雨量计观测值和雷达估测值插值得到的面雨量分布情况。结果表明:采用缺省的Z-R关系估算出的区域面雨量普遍偏小很多,优化后雷达估测的雨强分布与雨量站点测量的雨强分布更接近,且雷达定量估测区域面雨量的精度有了较大提高。

Accurate Registration of Remote Sensing Images Based on Local Optimal Transformation

As the basic work of image stitching and object recognition,image registration played an important part in the image processing field.Much previous work in registration accuracy and realtime performance progressed very slowly,especially in registrating images with line feature.An innovative method for image registration based on lines is proposed,it can effectively improve the accuracy and real-time performance of image registration.The line feature can deal with some registration problems where point feature does not work.Our registration process is divided into two parts.The first part determines the rough registration transformation relation between reference image and test image.Then the similarity degree among different transformation and modified nonmaximum suppression(MNMS)algorithms are obtained,which produce local optimal solution to optimize the rough registration transformation.The final optimal registration relation can be obtained from two registration parts according to the match scores.The experimental results show that the proposed method makes a more accurate registration relation and performs better in real-time situation.

基于系统结构的水利高质量发展内涵与结构解析

准确把握水利高质量发展内涵,是实施新时代水利发展战略的重要前提。通过系统梳理高质量发展和水利发展的研究进展,基于系统观,分析了水利高质量发展的基本概念体系和核心要义;基于系统结构及系统优化视角,构建了由水利高质量发展目标、发展途径、发展条件三要素组成的水利高质量发展系统的关系结构。结果表明:水利高质量发展既是水利系统优化的发展理念,也是实现系统治水的发展措施;将水利高质量发展目标解析为水旱灾害防御安全、水资源供给安全、水生态保护安全和水环境保护安全保障以及高质量水利共享;五大新发展理念内化于水利高质量发展系统的关系结构之中,其中水资源禀赋和绿色发展是前提基础和约束条件,开放是其外在环境条件和内在体现,水利科技创新、水资源优化配置、水资源集约节约利用、水利系统现代化管理、水利基础设施建设和水安全系统功能协调是发展的重要优化途径,高质量水安全保障与满足人民需求的高质量水利共享是发展目标的核心内容。基于系统结构及系统优化问题视角深入剖析水利高质量发展的系统结构及其内在关系,可进一步深化对水利高质量发展的内涵认识,为区域水利高质量发展综合定量评价奠定基础。

考虑水土互馈关系的区域水土资源联合优化配置

针对水土资源空间不匹配、水资源总量不足及土地资源利用程度不高等问题,以二元水循环理论为基础,构建考虑水土互馈关系的区域水土资源联合优化配置模型。该模型包括产水模块、水土联合配置模块和土地模拟模块,以GDP最大和基于生态绿当量的区域植被覆盖率(EGE-RVC)最高为目标函数,并提出了一种耦合约束法、逐次逼近法和非线性规划的三层嵌套算法进行求解。以河南省洛阳市为例对模型和方法进行验证,结果表明,洛阳市可利用水量增加了4463万m^(3),GDP提高了12.5%,EGE-RVC增加了2.6%,各用地类型缺水率不同程度下降。研究成果能够为区域水土资源优化配置研究提供新的思路和技术参考。

森林生态系统服务多功能性:概念、指标和经营模拟模型

森林生态系统同时提供多种功能和服务的能力,被称为森林生态系统多功能性,可分为生态系统功能多功能性和生态系统服务多功能性。近年来,生态学领域对生物多样性与多功能性关系机理、多功能性驱动因子等进行了大量研究,这些研究持续成为热点。然而,这些研究成果在实际应用,尤其是在指导森林经营实践方面,尚显不足。森林经营一直以实现多种功能或服务的最大化为目标,但目前还缺乏与生态学中多功能性理论研究的有机联系,迫切需要将二者结合。本文对国际国内森林生态系统服务多功能性的概念、指标、评价方法、经营模拟和优化模型进行了综述,认为预测森林生态系统服务及多功能性、在森林经营单位层面实现多功能性最大化是将来的研究方向。因此,需要加强森林经营模型模拟研究,寻找减少不同服务间冲突、增加协同(即增加多功能性)的经营策略,回答森林经营和气候变化如何影响生态系统服务间的权衡和协调关系及其时空变化等理论和实际问题。

宽体飞机地面主轮协同转弯控制律设计

主轮协同转弯技术能有效提高飞机的地面机动性,降低主起落架在转弯过程中受到的附加侧向扭矩,减小重载飞机地面转弯半径,但目前实现此技术的前主轮转角控制律设计方法尚不明确。提出以主起落架受力为约束条件,计算不同滑行速度下的前主轮转角关系,在全速度范围内进行分段选择并离散化,据此设计前主轮协同转弯控制律,以原理样机为对象进行仿真分析,验证了该控制律能有效降低两侧主起落架扭矩和的峰值。提出的设计方法对多轮系地面运载装备的复合转弯控制律设计具有一定的理论指导意义。

立德树人背景下导学关系现实困难与优化对策

导师是研究生培养的第一责任人,导学关系和谐程度直接关系着研究生育人成效。新时代立德树人背景下,导学关系正面临严峻考验。该文从优化研究生的招生和培养制度,提升导师育人能力,探索导学对话机制,强化立德树人根本任务意识方面,对克服导学关系现实困难提出应对策略。

基于帕累托前沿关系求解约束多目标优化问题

为解决约束多目标优化问题中的平衡约束满足与目标函数优化以及可行域复杂等挑战,提出了基于不同帕累托前沿关系的分类搜索方法。提出一种双种群双阶段框架:进化一个辅助种群Pa和一个主种群Pm,并将进化过程分为学习阶段和搜索阶段。学习阶段,种群Pa向UPF(unconstrained Pareto front)进行搜索,而种群Pm向CPF(constrained Pareto front)进行搜索,旨在探索UPF与CPF之间的关系;完成学习后,对不同问题的UPF与CPF关系进行分类,以指导后续搜索策略;在搜索阶段,根据不同的分类关系,调整种群Pa的搜索策略,旨在使种群Pa为种群Pm提供更有效的辅助信息。基于此算法框架,对不同类型约束多目标优化问题的帕累托前沿关系进行了分类,实现了对CPF更有效的搜索。实验结果表明:所提算法与其他7种先进的约束多目标优化算法相比具有更显著的性能优势。通过学习与利用UPF与CPF的关系,能够选择更合适的搜索策略去应对具有不同特性的约束多目标优化问题,以获得更具优势的最终解集。