Three novel acidic polysaccharide fractions(OFPP-1,OFPP-2,OFPP-3)with different m olecular weights(803.7,555.1 and 414.5 k Da)were isolated from the peeled Opuntia dillenii Haw.fruits by alkali-extraction,graded alcoh...Three novel acidic polysaccharide fractions(OFPP-1,OFPP-2,OFPP-3)with different m olecular weights(803.7,555.1 and 414.5 k Da)were isolated from the peeled Opuntia dillenii Haw.fruits by alkali-extraction,graded alcohol precipitation and column chromatography.Structural analysis indicated that OFPPs were pectic polysaccharides consisting of rhamnose,arabinose and galactose residues.The backbone of OFPP-1 consisted of a repeating unit→6-α-D-Galp A-(1→2)-α-L-Rhap-(1→with T-α-D-Galp A-(1→6)-α-D-Galp A-(1→4)-α-D-Glcp-(1→,T-β-D-Xylp-(1→6)-α-D-Galp A-(1→4)-α-D-Glcp-(1→or T-α-D-Galp A-(1→3)-α-L-Araf-(1→as the side chains.The backbone of OFPP-2 consisted of a disaccharide repeating unit→2)-α-L-Rhap-(1→4)-β-D-Galp A-(1→with T-β-L-Araf-(1→as the branches substituted at the O-4 position of→2,4)-α-LRhap-(1→.Whereas the backbone of OFPP-3 was→2,4)-α-L-Rhap-(1→2)-α-L-Rhap-(1→3)-β-L-Araf-(1→or→2,4)-α-L-Rhap-(1→2)-α-L-Rhap-(1→4)-β-D-Galp A-(1→,which was branched at the O-4 position of→2,4)-α-L-Rhap-(1→.Moreover,these three polysaccharide fractions could protect Huh-7 cells against H2O2-induced oxidative stress to different extents by decreasing the MDA content and increasing the SOD,CAT,GSH-Px activities and the GSH level in the Huh-7 cells.These results suggest that OFPPs have the potential to be used as natural antioxidants.展开更多
该文采用微波辅助法提取米邦塔仙人掌多糖,并与热回流提取方法进行比较。通过设计正交试验,得出优化的工艺条件:以水为提取剂,料液比1∶10(w/v),提取2次,每次提取3 m in,微波炉功率700 W,选用70%乙醇沉淀。微波提取粗多糖的得率和含量...该文采用微波辅助法提取米邦塔仙人掌多糖,并与热回流提取方法进行比较。通过设计正交试验,得出优化的工艺条件:以水为提取剂,料液比1∶10(w/v),提取2次,每次提取3 m in,微波炉功率700 W,选用70%乙醇沉淀。微波提取粗多糖的得率和含量分别为6.8%和8.5%,高于热回流提取粗多糖的得率4.7%和含量8.3%。微波提取不仅缩短了提取时间,降低提取剂用量,而且提高了仙人掌多糖得率。展开更多
目的分离、纯化具有降血糖作用的仙人掌多糖组分。方法经热水提取、乙醇沉淀、DEAE Sepharose fast flow离子交换色谱和Sephadex G系列凝胶滤过色谱纯化得到5种仙人掌多糖组分。醋酸纤维薄膜电泳检测多糖纯度,凝胶色谱测定相对分子质量...目的分离、纯化具有降血糖作用的仙人掌多糖组分。方法经热水提取、乙醇沉淀、DEAE Sepharose fast flow离子交换色谱和Sephadex G系列凝胶滤过色谱纯化得到5种仙人掌多糖组分。醋酸纤维薄膜电泳检测多糖纯度,凝胶色谱测定相对分子质量,高效液相色谱测定糖的组成。结果5种多糖都已达到电泳纯。它们的相对分子质量依次为2.0×103、1.0×106、4.0×103、9.2×105、5.0×103。ODP1由鼠李糖组成,ODP2可能由鼠李糖和葡萄糖组成,ODP4可能由鼠李糖和D-半乳糖组成。ODP3和ODP5分别由鼠李糖和一未知糖组分组成。结论仙人掌多糖的提取没有必要采用脱蛋白、脱色素步骤,本实验所提取的仙人掌多糖是均一的。展开更多
对热水提取米邦塔仙人掌(Opuntia milpa alta Haw)粗多糖的提取工艺进行研究。以新鲜米邦塔仙人掌为原料,采用单因素试验考察料液比、提取温度、提取时间和提取次数对米邦塔仙人掌粗多糖得率的影响,并通过响应面法优化工艺参数。结果表...对热水提取米邦塔仙人掌(Opuntia milpa alta Haw)粗多糖的提取工艺进行研究。以新鲜米邦塔仙人掌为原料,采用单因素试验考察料液比、提取温度、提取时间和提取次数对米邦塔仙人掌粗多糖得率的影响,并通过响应面法优化工艺参数。结果表明,影响粗多糖得率的因素主次顺序为提取温度>料液比>提取时间。确定最佳提取工艺参数为提取温度83℃,料液比1∶28(g∶m L),提取时间为3 h,在此条件下,仙人掌粗多糖的得率为2.05%。展开更多
目的分离纯化仙人掌多糖主要组分,观察仙人掌多糖主要组分(ODP-Ⅰa)对红细胞脂质过氧化损伤的影响,探讨其抗氧化机制。方法从野生仙人掌Opuntia dilleniiHaw.中采用超声提取法并用DEAE-Sepharose Fast Flow柱层析纯化得到仙人掌多糖组分...目的分离纯化仙人掌多糖主要组分,观察仙人掌多糖主要组分(ODP-Ⅰa)对红细胞脂质过氧化损伤的影响,探讨其抗氧化机制。方法从野生仙人掌Opuntia dilleniiHaw.中采用超声提取法并用DEAE-Sepharose Fast Flow柱层析纯化得到仙人掌多糖组分ODP-Ⅰa、ODP-Ⅰb和ODP-Ⅱ′。以活性氧(H2O2)作用于离体正常大鼠红细胞,观察ODP-Ⅰa对红细胞溶血率、高铁血红蛋白含量、丙二醛含量、超氧化物歧化酶和谷胱甘肽过氧化物酶活性、红细胞膜蛋白巯基含量和膜蛋白质高分子聚合物的影响。结果 ODP-Ⅰa能够抑制H2O2诱导的红细胞溶血和高铁血红蛋白的产生;提高H2O2氧化损伤红细胞中超氧化物歧化酶活性、谷胱甘肽过氧化物酶活性和降低丙二醛含量;同时,能够抑制H2O2引起的膜蛋白巯基含量的降低和红细胞膜蛋白高分子聚合物的形成。结论 ODP-Ⅰa对活性氧引起的红细胞脂质过氧化损伤具有保护作用。展开更多
比较了超声破碎时间、料液比、pH值对提取率仙人掌粗多糖(Polysaccharides from Opuntia dillenii Haw.,OPS)及活性的影响.结果表明,各因子对OPS的影响程度依次为pH值、超声破碎时间、料水比,影响OPS活性的主要因子是pH值,在酸性或碱性...比较了超声破碎时间、料液比、pH值对提取率仙人掌粗多糖(Polysaccharides from Opuntia dillenii Haw.,OPS)及活性的影响.结果表明,各因子对OPS的影响程度依次为pH值、超声破碎时间、料水比,影响OPS活性的主要因子是pH值,在酸性或碱性环境下提取到的仙人掌粗多糖活性较低.据回归模型及其期望函数途径进行模拟选优,并进行验证,得到OPS提取率的优化条件为超声破碎时间38 min左右,料液比1:36(g.mL-1),pH值7.16左右.展开更多
基金supported by the National Natural Science Foundation of China(No.31972977)。
文摘Three novel acidic polysaccharide fractions(OFPP-1,OFPP-2,OFPP-3)with different m olecular weights(803.7,555.1 and 414.5 k Da)were isolated from the peeled Opuntia dillenii Haw.fruits by alkali-extraction,graded alcohol precipitation and column chromatography.Structural analysis indicated that OFPPs were pectic polysaccharides consisting of rhamnose,arabinose and galactose residues.The backbone of OFPP-1 consisted of a repeating unit→6-α-D-Galp A-(1→2)-α-L-Rhap-(1→with T-α-D-Galp A-(1→6)-α-D-Galp A-(1→4)-α-D-Glcp-(1→,T-β-D-Xylp-(1→6)-α-D-Galp A-(1→4)-α-D-Glcp-(1→or T-α-D-Galp A-(1→3)-α-L-Araf-(1→as the side chains.The backbone of OFPP-2 consisted of a disaccharide repeating unit→2)-α-L-Rhap-(1→4)-β-D-Galp A-(1→with T-β-L-Araf-(1→as the branches substituted at the O-4 position of→2,4)-α-LRhap-(1→.Whereas the backbone of OFPP-3 was→2,4)-α-L-Rhap-(1→2)-α-L-Rhap-(1→3)-β-L-Araf-(1→or→2,4)-α-L-Rhap-(1→2)-α-L-Rhap-(1→4)-β-D-Galp A-(1→,which was branched at the O-4 position of→2,4)-α-L-Rhap-(1→.Moreover,these three polysaccharide fractions could protect Huh-7 cells against H2O2-induced oxidative stress to different extents by decreasing the MDA content and increasing the SOD,CAT,GSH-Px activities and the GSH level in the Huh-7 cells.These results suggest that OFPPs have the potential to be used as natural antioxidants.
文摘该文采用微波辅助法提取米邦塔仙人掌多糖,并与热回流提取方法进行比较。通过设计正交试验,得出优化的工艺条件:以水为提取剂,料液比1∶10(w/v),提取2次,每次提取3 m in,微波炉功率700 W,选用70%乙醇沉淀。微波提取粗多糖的得率和含量分别为6.8%和8.5%,高于热回流提取粗多糖的得率4.7%和含量8.3%。微波提取不仅缩短了提取时间,降低提取剂用量,而且提高了仙人掌多糖得率。
文摘目的分离、纯化具有降血糖作用的仙人掌多糖组分。方法经热水提取、乙醇沉淀、DEAE Sepharose fast flow离子交换色谱和Sephadex G系列凝胶滤过色谱纯化得到5种仙人掌多糖组分。醋酸纤维薄膜电泳检测多糖纯度,凝胶色谱测定相对分子质量,高效液相色谱测定糖的组成。结果5种多糖都已达到电泳纯。它们的相对分子质量依次为2.0×103、1.0×106、4.0×103、9.2×105、5.0×103。ODP1由鼠李糖组成,ODP2可能由鼠李糖和葡萄糖组成,ODP4可能由鼠李糖和D-半乳糖组成。ODP3和ODP5分别由鼠李糖和一未知糖组分组成。结论仙人掌多糖的提取没有必要采用脱蛋白、脱色素步骤,本实验所提取的仙人掌多糖是均一的。
