OBJECTIVE To investigate the significance and relationship between matrix metalloproteinase 9 (MMP-9) and infiltration of macrophages in the process of invasion and metastasis in oral squamous cell carcinoma (OSCC...OBJECTIVE To investigate the significance and relationship between matrix metalloproteinase 9 (MMP-9) and infiltration of macrophages in the process of invasion and metastasis in oral squamous cell carcinoma (OSCC). METHODS The immunohistochemical SABC method was used to detect the expression of MMP-9 and CD68 (for labeling macrophages) in 42 cases of OSCC and in 10 normal tissues. RESULTS The expression of MMP-9 and macrophage counts in the OSCC cases were significantly higher compared to normal tissues (P〈0.05). The expression of MMP-9 and macrophage counts were related to lymphnode metastasis and the TNM stage (P〈0.05), showing that there was a positive correlation among these parameters (y = 0.443, ,0〈0.01). CONCLUSION Both MMP-9 and macrophages may play an important role in the process of invasion and metastasis in OSCC, and this cellular activity may relate to the macrophages which affect the tumor cells and upregulate the expression of MMP-9.展开更多
目的探讨基质金属蛋白酶(matrix metalloproteinases,MMP)在口腔鳞状细胞癌(oral squamous cell carci noma,OSCC)中的表达及对其生物学行为的影响。方法免疫组化检测口腔癌和正常口腔组织中M M P-2和M M P-9的表达情况;分析高表达MMP-2...目的探讨基质金属蛋白酶(matrix metalloproteinases,MMP)在口腔鳞状细胞癌(oral squamous cell carci noma,OSCC)中的表达及对其生物学行为的影响。方法免疫组化检测口腔癌和正常口腔组织中M M P-2和M M P-9的表达情况;分析高表达MMP-2和MMP-9和临床颈淋巴结转移之间的关联;划痕实验检测MMP-2和MMP-9的表达对口腔鳞状细胞癌细胞迁移能力的影响;Transwell试验检测MMP-2和MMP-9的表达对口腔鳞状细胞癌细胞侵袭能力的影响。结果与正常口腔组织相比,口腔癌组织中MMP-2和MMP-9的表达明显上调;MMP-2和MMP-9的高表达与临床患者颈淋巴结转移有正相关关系;抑制MMP-2和MMP-9的表达可以抑制对口腔鳞状细胞癌细胞的迁移侵袭能力。结论 MMP-2和MMP-9在口腔癌中表达明显升高,抑制MMP-2和MMP-9的表达可以调控口腔鳞状细胞癌的生物学行为。展开更多
目的探讨宫颈鳞癌(SCC)中巨噬细胞移动抑制因子(MIF)、金属蛋白酶‐9(MMP‐9)和血管内皮生长因子(VEGF)的表达及意义。方法选择SCC组织标本51份(SCC组)、宫颈上皮内瘤样病变(CIN)标本41份(CIN组),正常宫颈组织(NCE)标...目的探讨宫颈鳞癌(SCC)中巨噬细胞移动抑制因子(MIF)、金属蛋白酶‐9(MMP‐9)和血管内皮生长因子(VEGF)的表达及意义。方法选择SCC组织标本51份(SCC组)、宫颈上皮内瘤样病变(CIN)标本41份(CIN组),正常宫颈组织(NCE)标本32份(NCE组),采用免疫组化SP法检测各组MIF 、MMP‐9和VEGF的表达并比较分析。结果 NCE组、CIN组、SCC组MIF、MMP‐9、VEGF阳性率及微血管密度(MVD)值依次升高,组间比较有统计学差异( P <0.05),且SCC组显著高于CIN组、NCE组;MIF、MMP‐9和VEGF在SCC组织中的阳性表达均与组织分化程度、有无淋巴结转移、临床分期有关( P <0.05);MIF、MMP‐9和VEGF阳性表达的M VD值与SCC临床分期、分化程度、淋巴结转移显著相关( P <0.05);在SCC中M IF和M M P‐9的表达呈显著正相关( P <0.05),而VEGF和MIF、MMP‐9的表达无相关性( P >0.05)。结论 MIF、MMP‐9和 VEGF在SCC的发生、发展、浸润及转移中起重要作用;SCC组织学分化越差,MIF、MMP‐9和VEGF阳性表达率及MVD值越高,提示三者联合检测可为评估SCC严重程度。展开更多
基金the National Natural Science Foundation of China (No. 30660200)the Provincial Governor’s Special Fund of Guizhou Province, China (No. 005-160).
文摘OBJECTIVE To investigate the significance and relationship between matrix metalloproteinase 9 (MMP-9) and infiltration of macrophages in the process of invasion and metastasis in oral squamous cell carcinoma (OSCC). METHODS The immunohistochemical SABC method was used to detect the expression of MMP-9 and CD68 (for labeling macrophages) in 42 cases of OSCC and in 10 normal tissues. RESULTS The expression of MMP-9 and macrophage counts in the OSCC cases were significantly higher compared to normal tissues (P〈0.05). The expression of MMP-9 and macrophage counts were related to lymphnode metastasis and the TNM stage (P〈0.05), showing that there was a positive correlation among these parameters (y = 0.443, ,0〈0.01). CONCLUSION Both MMP-9 and macrophages may play an important role in the process of invasion and metastasis in OSCC, and this cellular activity may relate to the macrophages which affect the tumor cells and upregulate the expression of MMP-9.
文摘目的探讨宫颈鳞癌(SCC)中巨噬细胞移动抑制因子(MIF)、金属蛋白酶‐9(MMP‐9)和血管内皮生长因子(VEGF)的表达及意义。方法选择SCC组织标本51份(SCC组)、宫颈上皮内瘤样病变(CIN)标本41份(CIN组),正常宫颈组织(NCE)标本32份(NCE组),采用免疫组化SP法检测各组MIF 、MMP‐9和VEGF的表达并比较分析。结果 NCE组、CIN组、SCC组MIF、MMP‐9、VEGF阳性率及微血管密度(MVD)值依次升高,组间比较有统计学差异( P <0.05),且SCC组显著高于CIN组、NCE组;MIF、MMP‐9和VEGF在SCC组织中的阳性表达均与组织分化程度、有无淋巴结转移、临床分期有关( P <0.05);MIF、MMP‐9和VEGF阳性表达的M VD值与SCC临床分期、分化程度、淋巴结转移显著相关( P <0.05);在SCC中M IF和M M P‐9的表达呈显著正相关( P <0.05),而VEGF和MIF、MMP‐9的表达无相关性( P >0.05)。结论 MIF、MMP‐9和 VEGF在SCC的发生、发展、浸润及转移中起重要作用;SCC组织学分化越差,MIF、MMP‐9和VEGF阳性表达率及MVD值越高,提示三者联合检测可为评估SCC严重程度。