Objective： To investigate the immunological function of a yeast expression system for thymosin α1 （Tα1）. Methods： A constructed Tetl yeast expression system was used to investigate the immunological function of ...Objective： To investigate the immunological function of a yeast expression system for thymosin α1 （Tα1）. Methods： A constructed Tetl yeast expression system was used to investigate the immunological function of orally administered Tα1. Dried yeast containing three different concentration of Tα1 was fed to normal Balb/c mice and other Balb/c mice whose immunities were inhibited in advance by cyclophosphamide. Synthesized Tα1 peptide was used as positive control and dried yeast with empty plasmid was used as negative control. CD4^＋ and CD8^＋ levels were detected by flow cytometry assay. TNF-α, IFN-γ, IL-2, IL-6 and IL-10 levels were detected by liquid chip. Results： In normal Balb/c mice or immune inhibition Balb/c mice, CD8^＋ levels were significantly increased. Especially in immune inhibition Balb/c mice, CD8^＋ levels in synthesized Tα1 group （18.77%±4.72%）, small dose group （13.48%±6.17%） and large dose group （22.74%±1.09%） were significantly higher than that in empty yeast control group （7.49%±2.14%）. Conclusion： Orally administered Tα1 has its certain immunomodulatory function.展开更多
基金Project (No. 2003C13015) supported by the Key Project from Sci-ence and Technology Department of Zhejiang Province, China
文摘Objective： To investigate the immunological function of a yeast expression system for thymosin α1 （Tα1）. Methods： A constructed Tetl yeast expression system was used to investigate the immunological function of orally administered Tα1. Dried yeast containing three different concentration of Tα1 was fed to normal Balb/c mice and other Balb/c mice whose immunities were inhibited in advance by cyclophosphamide. Synthesized Tα1 peptide was used as positive control and dried yeast with empty plasmid was used as negative control. CD4^＋ and CD8^＋ levels were detected by flow cytometry assay. TNF-α, IFN-γ, IL-2, IL-6 and IL-10 levels were detected by liquid chip. Results： In normal Balb/c mice or immune inhibition Balb/c mice, CD8^＋ levels were significantly increased. Especially in immune inhibition Balb/c mice, CD8^＋ levels in synthesized Tα1 group （18.77%±4.72%）, small dose group （13.48%±6.17%） and large dose group （22.74%±1.09%） were significantly higher than that in empty yeast control group （7.49%±2.14%）. Conclusion： Orally administered Tα1 has its certain immunomodulatory function.
