Proliferation and adipogenic differentiation of human adipose-derived stem cells isolated from middle-aged patients with prominent orbital fat in the lower eyelids

Aim:To evaluate the age-related effects on the adipogenic differentiation and proliferation potentials of human orbital adipose-derived stem cells(OASCs).Methods:Orbital adipose samples were harvested from the central fat compartment in the lower eyelids of 10 young and middle-aged patients during routine blepharoplasty surgery.After assessment of the morphological changes of adipocytes with aging,OASCs were isolated from the fat samples and expanded in vitro.Differences in the stem cell colony number(fibroblast colony-forming unit),growth rate and phenotype characterization(flow cytometry analysis)were evaluated.The ability of OASCs to differentiate into adipocytes was determined by oil red O staining and the mRNA expression level of peroxisome proliferator-activated receptorγ.Results:Fat cell size showed a decreasing trend with advancing age.Although no difference was found in the expression of cell surface markers,the colony number and proliferative rate of OASCs from middle-aged donors were significantly lower than those from the young donors.The adipogenic differentiation capacity of middle-aged OASCs was also reduced.These differences were statistically significant(P<0.001).Conclusion:The data showed that the progenitor cell number,proliferation capacity and adipogenic potential of OASCs decreased with aging,suggesting that using OASCs from elderly patients for therapeutic purposes might be restricted.

球后脂肪体积测定对甲状腺相关性眼病治疗时机的判定价值

目的探讨商业化软件计算球后脂肪体积,分析其与甲状腺相关性眼病(TAO)进展及预后的关系。方法收集2016年1月~2016年12月在我院内分泌科确诊的35例(70个眼眶)TAO患者的临床资料。测量1.5T眼眶MRI球后脂肪体积及眼外肌信号强度比值(SIR)分析其与临床各项指标的相关性,并收集12例(24个眼眶)健康人测量球后脂肪体积,初步比较TAO组及健康组体积的差异。结果脂肪体积与病程成正相关(r=0.480,P<0.01),病程6个月以内组与6~12个月组相比,脂肪体积差异不显著(P=0.084)。病程6个月以内组及病程6~12个月组球后脂肪体积均显著低于病程大于12个月组(P<0.01,P<0.05)。脂肪体积与突眼度存在相关性(r=0.622,P<0.01),突眼度每增加1 mm,球后脂肪体积将增加0.88 m L。临床活动性评分(CAS)与SIR值及促甲状腺素受体抗体(TRAb)存在相关性(r=0.536,r=0.416,P<0.01)。TAO组球后脂肪体积显著高于正常组(P<0.01)。结论 TAO病程1年以上可能是球后脂肪组织增多的高峰阶段,球后脂肪体积结合SIR值的测量有助于最佳激素治疗时机的探索及预后分析。

眼轮匝肌瓣联合眶隔脂肪重置矫治上睑凹陷效果观察

目的:探讨眼轮匝肌瓣联合眶隔脂肪重置治疗上睑凹陷的临床效果。方法:笔者科室对收治的30例上睑凹陷就医者,采用常规重睑成形的手术切口,但将重睑成形术中本应去除的组织(眼轮匝肌瓣)作为充填物,使其反折向上或自然回缩,同时释放眶隔脂肪填充至凹陷区域,合并轻中度上睑下垂者采用联合腱膜缩短术,最后以常规切开重睑成形法缝合手术切口。结果:术后3、6个月,总有效率均达96%(29/30),其中1例严重上睑凹陷就医者,因眼轮匝肌菲薄,睁眼时凹陷改善效果不太理想,后期行颗粒脂肪填充,最终达到满意效果。结论:对于上睑凹陷的就医者,眼轮匝肌瓣充填和眶隔脂肪重置能够增加上睑凹陷区域的组织容量,一期进行上睑下垂和内眦赘皮的矫正,眼部外形美观自然,术后无明显并发症,优于常规上睑凹陷的解决方案,值得临床推广应用。

眼轮匝肌瓣联合眶隔脂肪瓣矫正上睑凹陷的临床应用及美学效果分析

目的:探讨眼轮匝肌瓣联合眶隔脂肪瓣矫正上睑凹陷的临床应用及美学效果。方法:选择2016年7月-2017年7月本院收治的100例上睑凹陷患者随机分为对照组(50例)和观察组(50例)。对照组给予传统眼睑肌瓣矫正术治疗,观察组给予眼轮匝肌瓣联合眶隔脂肪瓣矫正术治疗。观察比较两组患者治疗效果及治疗满意度。结果:治疗后,观察组患者重睑线自然流畅,上睑凹陷明显改善,美学效果显著提高。观察组总有效率为94.0%显著高于对照组的78.0%,差异有统计学意义(P<0.05);观察组总满意度为96.0%明显高于对照组的78.0%,差异具有统计学意义(P<0.05)。结论:眼轮匝肌瓣联合眶隔脂肪瓣矫正上睑凹陷效果显著,可明显提高患者满意度及美学效果,值得在临床中应用推广。