[Objectives]To explore the effects of injection of oregano oil submicron emulsion on lipopolysaccharide-induced pneumonia in rats.[Methods]Rats induced by lipopolysaccharide were used as animal models of acute pneumon...[Objectives]To explore the effects of injection of oregano oil submicron emulsion on lipopolysaccharide-induced pneumonia in rats.[Methods]Rats induced by lipopolysaccharide were used as animal models of acute pneumonia.The experiment was divided into blank group,model group,administration group and positive drug control group.The morphology of lung tissue and the changes of cells and inflammatory factors in each group were observed,and the anti-inflammatory effects of injection of oregano oil submicron emulsion.[Results]The injection of oregano oil submicron emulsion can improve the pathological injury of rat lung tissue,inhibit the release of IL-6,IL-10,TNF-αcytokines induced by lipopolysaccharide,and significantly reduce the value of CRP.[Conclusions]Oregano oil submicron emulsion has a certain therapeutic effect on lipopolysaccharide-induced pneumonia in rats,and its mechanism may be related to reducing the release of cytoinflammatory factor IL-6,IL-10,and TNF-αand alleviating the injury to tissues and organs.展开更多
This experiment was conducted to investigate the effects of benzoic acid,Bacillus coagulans and oregano oil combined supplementation on growth performance,immune status and intestinal barrier integrity of piglets.In a...This experiment was conducted to investigate the effects of benzoic acid,Bacillus coagulans and oregano oil combined supplementation on growth performance,immune status and intestinal barrier integrity of piglets.In a 26-d experiment,25 piglets were randomly assigned to 5 treatments:1)a basal diet,negative control(NC),2)NC added with antibiotics,positive control(PC);3)NC added with benzoic acid at 3,000 g/t and Bacillus coagulans at 400 g/t(AB);4)NC added with benzoic acid at 3,000 g/t and o regano oil at 400 g/t(AO);5)NC added with 3,000 g/t benzoic acid and Bacillus coagulans at 400 g/t and oregano oil at 400 g/t(ABO);On d 27,all piglets were euthanized to obtain jejunal mucosa to measure immune status and intestinal barrier integrity.Results showed that pigs fed AB diet increased the final body weight and average daily body weight gain and decreased the ratio of feed to gain co mpared with NC group(P<0.05).Co mpared with NC group,AB,AO and ABO decreased serum tumor necrosis factor-a concentration and ABO decreased interleukin-1βconcentration in serum and jejunal mucosa(P<0.05).Compared with NC group,AB upregulated mRNA expressions of sodium-glucose cotransportel,claudin-1,occludin and mucin2 in jejunal mucosa and the populations of Bifidobacterium and Bacillus in cecal digesta(P<0.05).Compared with NC group,ABO increased jejunal mucosal occludin mRNA abundance and Bifidobacterium population in cecal digesta,and decreased Escherichia coli population in cecal digesta(P<0.05).Furthermore,AB and ABO increased Bacillus population in cecal digesta compared with PC group(P<0.05).These results indicated that dietary AB supplementation could improve growth performance and intestinal barrier integrity of piglets when fed antibiotic-free diets,which was possibly associated with the improvement of immune status and intestinal microflora.Dietary ABO supplementation is also beneficial to improve immune status and intestinal barrier integrity and micro flora of piglets.展开更多
This study was designed to investigate the effects of dietary oregano essential oils on 150 30-week-old Hy-Line Layers’ productive performance, egg quality characteristics, cecal microbiota, digestive enzyme activiti...This study was designed to investigate the effects of dietary oregano essential oils on 150 30-week-old Hy-Line Layers’ productive performance, egg quality characteristics, cecal microbiota, digestive enzyme activities, mucosa structure of the duodenum and jejunum and glucose and small peptides transporters expression in the duodenum and jejunum. All hens were allocated into one of five different groups: negative control (NC;basal diet only), antibiotics control (CS;basal diet plus 100 mg/kg of 10% colistin sulfate) and treatments I, II, and III (basal diet supplemented with 50, 100 and 150 mg/kg oregano essential oils, respectively). The results showed that the laying rate, average egg weight, feed conversion ratio and the activities of amylase and trypsin were significantly improved by a diet supplemented with 100 mg/kg oregano essential oils (P 0.05), significantly increased duodenum villus-height-to-crypt-depth ratios (P 0.05).展开更多
The product of plant essential oil, oregano carvacrol, was added in the diet of broiler, to study the effect of oregano carvacrol on growth performance of broilers. Using single factor test, 190 663 individuals of hea...The product of plant essential oil, oregano carvacrol, was added in the diet of broiler, to study the effect of oregano carvacrol on growth performance of broilers. Using single factor test, 190 663 individuals of healthy AA broilers at 0 day old were randomly divided into two groups, eight repeats in control group and four repeats in experimental group. The broilers in control group were fed with basal diet （containing 10 mg/kg cnramycin and 60 mg/kg salinomycin sodium）, and those in experimental groups were fed with the basal diet adding with 100 mg/kg oregano carvacrol. Compared with control group, the daily gain of broilers in exper- imental group was increased noticeably by 3.41 g/broiler （P 〈 0. 05） ; the slaughter quality was increased remarkably by 143 g/broiler （P 〈 0.05 ） ; the survival rate was increased significantly by 3.01% （ P 〈 0.05 ） ; and the feed gain ratio was reduced markedly by 0.08 （ P 〈 0.05 ）. The paper provided a theoretical basis for application of oregano carvacrol in broiler production.展开更多
An essential oil is the volatile lipophilic component extracted from plants. Microencapsulation systems protect the essential oil from degradation and evaporation, and, at the same time, allow a sustained release. Thi...An essential oil is the volatile lipophilic component extracted from plants. Microencapsulation systems protect the essential oil from degradation and evaporation, and, at the same time, allow a sustained release. This work analyses and characterizes the oregano and sage essential oil microcapsules prepared by interfacial polymerization technique, using polyurea as wall material. Several instrumental techniques are used: optical microscopy, size particle, Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), Termo gravimetric analysis (TGA), spectrophotometry, antimicrobial test and chromatography. Results show that oregano and sage oil have antimicrobial properties, and their microencapsulation allows knowing that these properties remain inside the microcapsules.展开更多
基金Supported by National Natural Science Foundation of China(81860771)Science and Technology Project of the Education Department of Jiangxi Province(GJJ201219,GJJ170722)+2 种基金Students Innovative Entrepreneurial Training Plan Program of Jiangxi University of Traditional Chinese Medicine(202010412006,202010412143)Program of Key Discipline Training of Young Teachers of Jiangxi University of Traditional Chinese Medicine(2017jzzdxk001)Scientific Research Foundation of Jiangxi University of Traditional Chinese Medicine(2018WBZR011).
文摘[Objectives]To explore the effects of injection of oregano oil submicron emulsion on lipopolysaccharide-induced pneumonia in rats.[Methods]Rats induced by lipopolysaccharide were used as animal models of acute pneumonia.The experiment was divided into blank group,model group,administration group and positive drug control group.The morphology of lung tissue and the changes of cells and inflammatory factors in each group were observed,and the anti-inflammatory effects of injection of oregano oil submicron emulsion.[Results]The injection of oregano oil submicron emulsion can improve the pathological injury of rat lung tissue,inhibit the release of IL-6,IL-10,TNF-αcytokines induced by lipopolysaccharide,and significantly reduce the value of CRP.[Conclusions]Oregano oil submicron emulsion has a certain therapeutic effect on lipopolysaccharide-induced pneumonia in rats,and its mechanism may be related to reducing the release of cytoinflammatory factor IL-6,IL-10,and TNF-αand alleviating the injury to tissues and organs.
基金supported by the National Key Research and Development Program of China(2018YFD0500605)the Science and Technology Support Program of Sichuan Province(2014NZ0043,2016NZ0006)
文摘This experiment was conducted to investigate the effects of benzoic acid,Bacillus coagulans and oregano oil combined supplementation on growth performance,immune status and intestinal barrier integrity of piglets.In a 26-d experiment,25 piglets were randomly assigned to 5 treatments:1)a basal diet,negative control(NC),2)NC added with antibiotics,positive control(PC);3)NC added with benzoic acid at 3,000 g/t and Bacillus coagulans at 400 g/t(AB);4)NC added with benzoic acid at 3,000 g/t and o regano oil at 400 g/t(AO);5)NC added with 3,000 g/t benzoic acid and Bacillus coagulans at 400 g/t and oregano oil at 400 g/t(ABO);On d 27,all piglets were euthanized to obtain jejunal mucosa to measure immune status and intestinal barrier integrity.Results showed that pigs fed AB diet increased the final body weight and average daily body weight gain and decreased the ratio of feed to gain co mpared with NC group(P<0.05).Co mpared with NC group,AB,AO and ABO decreased serum tumor necrosis factor-a concentration and ABO decreased interleukin-1βconcentration in serum and jejunal mucosa(P<0.05).Compared with NC group,AB upregulated mRNA expressions of sodium-glucose cotransportel,claudin-1,occludin and mucin2 in jejunal mucosa and the populations of Bifidobacterium and Bacillus in cecal digesta(P<0.05).Compared with NC group,ABO increased jejunal mucosal occludin mRNA abundance and Bifidobacterium population in cecal digesta,and decreased Escherichia coli population in cecal digesta(P<0.05).Furthermore,AB and ABO increased Bacillus population in cecal digesta compared with PC group(P<0.05).These results indicated that dietary AB supplementation could improve growth performance and intestinal barrier integrity of piglets when fed antibiotic-free diets,which was possibly associated with the improvement of immune status and intestinal microflora.Dietary ABO supplementation is also beneficial to improve immune status and intestinal barrier integrity and micro flora of piglets.
文摘This study was designed to investigate the effects of dietary oregano essential oils on 150 30-week-old Hy-Line Layers’ productive performance, egg quality characteristics, cecal microbiota, digestive enzyme activities, mucosa structure of the duodenum and jejunum and glucose and small peptides transporters expression in the duodenum and jejunum. All hens were allocated into one of five different groups: negative control (NC;basal diet only), antibiotics control (CS;basal diet plus 100 mg/kg of 10% colistin sulfate) and treatments I, II, and III (basal diet supplemented with 50, 100 and 150 mg/kg oregano essential oils, respectively). The results showed that the laying rate, average egg weight, feed conversion ratio and the activities of amylase and trypsin were significantly improved by a diet supplemented with 100 mg/kg oregano essential oils (P 0.05), significantly increased duodenum villus-height-to-crypt-depth ratios (P 0.05).
文摘The product of plant essential oil, oregano carvacrol, was added in the diet of broiler, to study the effect of oregano carvacrol on growth performance of broilers. Using single factor test, 190 663 individuals of healthy AA broilers at 0 day old were randomly divided into two groups, eight repeats in control group and four repeats in experimental group. The broilers in control group were fed with basal diet （containing 10 mg/kg cnramycin and 60 mg/kg salinomycin sodium）, and those in experimental groups were fed with the basal diet adding with 100 mg/kg oregano carvacrol. Compared with control group, the daily gain of broilers in exper- imental group was increased noticeably by 3.41 g/broiler （P 〈 0. 05） ; the slaughter quality was increased remarkably by 143 g/broiler （P 〈 0.05 ） ; the survival rate was increased significantly by 3.01% （ P 〈 0.05 ） ; and the feed gain ratio was reduced markedly by 0.08 （ P 〈 0.05 ）. The paper provided a theoretical basis for application of oregano carvacrol in broiler production.
文摘An essential oil is the volatile lipophilic component extracted from plants. Microencapsulation systems protect the essential oil from degradation and evaporation, and, at the same time, allow a sustained release. This work analyses and characterizes the oregano and sage essential oil microcapsules prepared by interfacial polymerization technique, using polyurea as wall material. Several instrumental techniques are used: optical microscopy, size particle, Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), Termo gravimetric analysis (TGA), spectrophotometry, antimicrobial test and chromatography. Results show that oregano and sage oil have antimicrobial properties, and their microencapsulation allows knowing that these properties remain inside the microcapsules.
