Recommendations for Using Crop Straws to Produce Organic Fertilizers in Liaoning Province

This paper introduces in detail the current situation of crop straw resources and comprehensive utilization in Liaoning Province and the basic situation of enterprises using crop straw to produce organic fertilizers.In addition,it analyzes in detail the problems existing in the production of organic fertilizers in Liaoning Province,and finally comes up with some recommendations for using crop straw to produce organic fertilizers.

Local Knowledge of Farmer Communities in the Use of Organic Fertilizers to Increase Cashew Production in Buton Utara Regency, Southeast Sulawesi Province, Indonesia

Local knowledge has an important role in agricultural practices.This study aims to describe the local knowledge of farming communities about the use of organic fertilizers as a strategy to increase cashew production in Buton Utara Regency,Southeast Celebes,Indonesia.The research method used is a qualitative approach with data collection techniques using in-depth interviews and observation.The research results showed that the farming community in this area has in-depth local knowledge of how to make and apply effective organic fertilizers sourced from the surrounding natural environment.However,there are challenges in access to the organic materials needed and constraints in the widespread implementation of organic fertilizers.This study recommends strengthening education and training programs to increase farmers’awareness and skills in using organic fertilizers.

Combined Application of Organic and Inorganic Fertilizers on Black Soil Fertility and Maize Yield

By two years （2007-2008） located fertilizer experiment, the effect of long-term combined application of organic and inorganic fertilizers on black soil fertility and crop yield was investigated in Shuangcheng City, Heilongjiang Province. The results showed that the combined application of organic and inorganic fertilizers could increase the organinc matter, alkaline nitrogen, available phosphorus and available potassium. At the same time, the increasing application of organic fertilizer could reduce the soil bulk density and improve the field moisture capacity. Field moisture capacity and organic matter of the combined application of high quantities of organic manure and inorganic fertilizers AtB5 treatment increased the fhstest, organic matter increased by 3.33 g. kg and field moisture capacity increased by 11.25% than the beginning of the experiment. Under the same fertilization, the combined application of organic and inorganic fertilizers＇ increasing production range was higher than the single chemical fertilizers＇ which was from 0.8% to 9.4%. The results showed that the combined application of organic and inorganic fertilizers could increase the nutrient contents of soil and also was the highest productivity contribution to black soil fertility. It was the best fertilization structure of increasing productivity level and improving the soil fertility.

Effects of Organic Fertilizers Made from Distiller＇s Grains on the Growth and Development of Flue-cured Tobacco

Pot experiments were conducted to study the effects of different organic fertilizers made from distiller＇s grains on the physiological characteristics and growth of flue-cured tobacco. The results showed that the agronomic traits and biomass of flue-cured tobacco in the three treatments were higher than that of the control group,and the mixed application of different organic fertilizers made from distiller＇s grains and compound fertilizer could promote the growth and development of flue-cured tobacco significantly.

Changes in Grain Yield of Rice and Emission of Greenhouse Gases from Paddy Fields after Application of Organic Fertilizers Made from Maize Straw

A field experiment was conducted at the farm of Yangzhou University, Yangzhou, China, to study the effects of organic fertilizers made from maize straw on rice grain yield and the emission of greenhouse gases. Four organic fertilizer treatments were as follows： maize straw （MS）, compost made from maize straw （MC）, methane-generating maize residue （MR）, and black carbon made from maize straw （BC）. These organic fertilizers were applied separately to paddy fields before rice transplanting. No organic fertilizer was applied to the control （CK）. The effects of each organic fertilizer on rice grain yield and emission of greenhouse gases were investigated under two conditions, namely, no nitrogen （N） application （ON） and site-specific N management （SSNM）. Rice grain yields were significantly higher in the MS, MC and MR treatments than those in CK under either ON or SSNM. The MS treatment resulted in the highest grain yield and agronomic N use efficiency. However, no significant difference was observed for these parameters between the BC treatment and CK. The changes in the emissions of methane （CH4） carbon dioxide （CO2）, or nitrous oxide （N20） from the fields were similar among all organic fertilizer treatments during the entire rice growing season. The application of each organic fertilizer significantly increased the emission of each greenhouse gas （except N20 emission in the BC treatment） and global warming potential （GWP）. Emissions of all the greenhouse gases and GWP increased under the same organic fertilizer treatment in the presence of N fertilizer, whereas GWP per unit grain yield decreased. The results indicate that the application of organic fertilizer （MS, MC or MR） could increase grain yield, but also could enhance the emissions of greenhouse gases from paddy fields. High grain yield and environmental efficiency could be achieved by applying SSNM with MR.

Study on the Activation Effect of Fermented Organic Fertilizers on Selenium in the Soil of Southern Dragon Fruit Orchards

[Objectives]This study was conducted to explore the effects of using fermented organic fertilizers such as cow manure on the activity of selenium in the soil.[Methods]The project team applied the organic fertilizer prepared by fermenting cow manure and other raw materials to activate selenium in the soil at two dragon fruit orchard bases in Shilong Town,Guiping City and Dingdang Town,Long an County,and carried out many years of experiments.[Results]The contents of available selenium in the two experimental orchards in Shilong Town and Dingdang Town increased by 10%and 5%,respectively,and the selenium contents in dragon fruit increased by 228.6%and 200.0%,respectively.[Conclusions]The application of fermented organic fertilizers such as cow manure can increase available selenium content in the soil,thereby increasing the selenium content of dragon fruit.

Improvement of Wolfberry(Lycium barbarum L.)Fruit Yield and Quality and Enhancement of Soil Fertility by Nitrogen Reduction Combined with Organic Fertilizers

Excessive amounts of nitrogen(N)fertilizers are applied during wolfberry production,resulting in some soil problems as well as potential environmental risks in the Qinghai-Tibet Plateau.In this study,organic fertilizers were used to replace part of the N fertilizer in wolfberry fields with different fertility levels.N fertilizer rates had 0,50,100,150,200,and 250 g N/plant.Organic fertilizer rates had 0,2,4,6,8,and 10 kg organic fertilizer/plant.The experimental treatments included 6 combinations of N0M10,N50M8,N100M6,N150M4,N200M2,and control was N250M0.The results showed that in the high-fertility soils,combinations of N150M4,N100M6 and N50M8 treatments were increased in yields,fruit shape index,flavonoid content,total phenol content,mineral nutrient content,and antioxidant activity of wolfberry fruits.Also they were improved in soil fertility and decreased in residual nitrate through the soil depth of 0-300 cm.In the soil with less fertility,fruit yield,amino acid contents,flavonoids,total phenols,mineral nutrients and antioxidant activity of fruits were increased by the N200M2,N150M4 and N100M6 treatments and soil fertility was improved as well.Also more residual nitrate was found in the depth of 0-100 cm of soil with both chemical and organic fertilizer compared with the control.Therefore,in the Qinghai-Tibet Plateau,combining decreased N fertilizer with organic fertilizer rather than chemical fertilizer alone could help farmers achieve satisfactory yields and quality of wolfberry fruits and reduce the risk of nitrate leaching.In conclusion,50-150 g/plant of N fertilizer combined with 4-8 kg/plant of organic fertilizer in high-fertility gardens and 100-200 g/plant of N fertilizer combined with 2-6 kg/plant of organic fertilizer in low-fertility gardens are recommended for wolfberry cultivation.

Coupling of reduced inorganic fertilizer with plant-based organic fertilizer as a promising fertilizer management strategy for colored rice in tropical regions

Colored rice is a type of high-quality,high-added-value rice that has attracted increasing attention in recent years.The use of large amounts of inorganic nitrogen fertilizer in rice fields results in low fertilizer use efficiency and high environmental pollution.Organic fertilizer is a promising way to improve soil quality and sustain high yields.However,most studies focus on the effect of animal-based organic fertilizers.The effects of different ratios of plantbased organic fertilizer and inorganic fertilizer on the grain yield and quality of colored rice have rarely been reported.Therefore,a two-year field experiment was conducted in 2020 and 2021 to study the effects of replacing inorganic N fertilizers with plant-based organic fertilizers on the yield,nitrogen use efficiency(NUE),and anthocyanin content of two colored rice varieties in a tropical region in China.The experimental treatments included no nitrogen fertilization(T1),100% inorganic nitrogen fertilizer(T2),30%inorganic nitrogen fertilizer substitution with plant-based organic fertilizer(T3),60%inorganic nitrogen fertilizer substitution with plant-based organic fertilizer(T4),and 100% plantbased organic fertilizer(T5).The total nitrogen provided to all the treatments except T1 was the same at 120 kg ha-1.Our results showed that the T3 treatment enhanced the grain yield and anthocyanin content of colored rice by increasing nitrogen use efficiency compared with T2.On average,grain yields were increased by 9 and 8%,while the anthocyanin content increased by 16 and 10% in the two colored rice varieties under T3 across the two years,respectively,as compared with T2.Further study of the residual effect of partial substitution of inorganic fertilizers showed that the substitution of inorganic fertilizer with plant-based organic fertilizer improved the soil physiochemical properties,and thus increased the rice grain yield,in the subsequent seasons.The highest grain yield of the subsequent rice crop was observed under the T5 treatment.Our results suggested that the application of plantbased organic fertilizers can sustain the production of colored rice with high anthocyanin content in tropical regions,which is beneficial in reconciling the relationship between rice production and environmental protection.

IMPACTS OF TECHNICAL ENVIRONMENT ON THE ADOPTION OF ORGANIC FERTILIZERS AND BIOPESTICIDES AMONG FARMERS:EVIDENCE FROM HEILONGJIANG PROVINCE,CHINA

Excessive application of mineral fertilizers and synthetic pesticides poses a substantial threat to the soil and water environment and food security.Organic fertilizer and biopesticides have gradually become essential technology for reducing mineral fertilizer and pesticide inputs.In the process,the technical environment is critical for promoting farmer behavior related to the adoption of organic fertilizer and biopesticides.This paper analyzes the influence of the technical environment on farmer behavior related to the adoption of organic fertilizer and biopesticides based on a survey of 1282 farmers in Heilongjiang Province,China,using the bivariate probit model.The results indicate that(1)farmer behavior related to the adoption applying organic fertilizer and biopesticides were positively correlated;(2)the technical environment had a significant positive impact on farmer behavior related to the adoption of organic fertilizer and biopesticides;and(3)the technical environment had a heterogeneous effect across different groups of farmers.This research provides insights useful for promoting organic fertilizer and biopesticides to farmers.It can be helpful to bundle relevant environmental technologies,conduct technology training for farmers and strengthen the construction of rural information networks.

Combined application of organic fertilizer and chemical fertilizer alleviates the kernel position effect in summer maize by promoting post-silking nitrogen uptake and dry matter accumulation

Adjusting agronomic measures to alleviate the kernel position effect in maize is important for ensuring high yields.In order to clarify whether the combined application of organic fertilizer and chemical fertilizer(CAOFCF)can alleviate the kernel position effect of summer maize,field experiments were conducted during the 2019 and 2020 growing seasons,and five treatments were assessed:CF,100%chemical fertilizer;OFCF1,15%organic fertilizer+85%chemical fertilizer;OFCF2,30%organic fertilizer+70%chemical fertilizer;OFCF3,45%organic fertilizer+55%chemical fertilizer;and OFCF4,60%organic fertilizer+40%chemical fertilizer.Compared with the CF treatment,the OFCF1 and OFCF2 treatments significantly alleviated the kernel position effect by increasing the weight ratio of inferior kernels to superior kernels and reducing the weight gap between the superior and inferior kernels.These effects were largely due to the improved filling and starch accumulation of inferior kernels.However,there were no obvious differences in the kernel position effect among plants treated with CF,OFCF3,or OFCF4 in most cases.Leaf area indexes,post-silking photosynthetic rates,and net assimilation rates were higher in plants treated with OFCF1 or OFCF2 than in those treated with CF,reflecting an enhanced photosynthetic capacity and improved postsilking dry matter accumulation(DMA)in the plants treated with OFCF1 or OFCF2.Compared with the CF treatment,the OFCF1 and OFCF2 treatments increased post-silking N uptake by 66.3 and 75.5%,respectively,which was the major factor driving post-silking photosynthetic capacity and DMA.Moreover,the increases in root DMA and zeatin riboside content observed following the OFCF1 and OFCF2 treatments resulted in reduced root senescence,which is associated with an increased post-silking N uptake.Analyses showed that post-silking N uptake,DMA,and grain yield in summer maize were negatively correlated with the kernel position effect.In conclusion,the combined application of 15-30%organic fertilizer and 70-85%chemical fertilizer alleviated the kernel position effect in summer maize by improving post-silking N uptake and DMA.These results provide new insights into how CAOFCF can be used to improve maize productivity.

Application of organic fertilizer for improving soybean production under acidic stress

The presence of acidic soil in rural areas poses difficulties for agricultural production.One factor regulating soil pH is the overuse of inorganic fertilizer.The increased use of fertilizers in soybean production not only raises sustainability concerns but also contributes to soil acidity.Therefore,the use of organic fertilizer could offer a solution for addressing both issues related to soil acidity and sustainability.The purpose of this study was to investigate the manipulation of soil pH using organic fertilizer for soybean production under acidic stress.The planting medium,consisting of a mixture of topsoil,rice husk charcoal,and organic fertilizer(in a ratio of 2:1:1),was supplemented with 0.5 g of NPK fertilizer as a basal treatment in each planting medium.To regulate the soil acidity to pH 4,we added FeSO_(4) and allowed the mixture to incubate for 30 days.The results demonstrate that the application of three types of organic fertilizers chicken manure(P1),oil palm empty bunch fertilizer(P2),and vermicompost(P3)positively impacts the growth of three soybean varieties.The findings indicate that the application of P2 organic fertilizer can increase vegetative growth almost 50%in soybeans on acidic soil,including plant height,leaf count,and root length.Meanwhile,applying P3 organic fertilizer can boost reproductive growth responses in soybeans on acidic soil,such as pod number(from around 0-4 unit to 42-51 unit),grain number(from around 0-5 unit to 88-90 unit),and grain weight(from around 0-0.37 g to 12-25 g).Organic fertilizer has the potential to regulate soil pH,promoting higher yields of soybeans under acidic stress.

Growth, Yield and Yield Characteristics of Three Pepper Cultivars to Fertilizers Application in the Mount Cameroon Region

This study determined the effects of organic and inorganic fertilizers on growth and yield of three pepper cultivars in the Mount Cameroon Region. Pepper seedlings were treated with six treatments (T1 = no fertilizers (control), T2 = 250 Kg/ha NPK 20:10:10, T3 = 350 Kg/ha NPK 20:10:10, T4 = 450 Kg/ha NPK 20:10:10, T5 = 20 Mg/ha poultry manure (PM), T6 = 30 Mg/ha PM). The effects of fertilizers had a significant effect on vegetative growth and yield of Capsicum chinense cv. “Big Sun” plants had the maximum plant height (39.09 cm) and mean collar diameter (6.02 mm) in plots treated with poultry manure at 20 Mg/ha (T5) and 30 Mg/ha (T6) respectively. The highest yield (33.63 Mg/ha) was in Capsicum chinense cv. Safi plants supplied with 30 Mg/ha poultry manure (T6) and the lowest yield (10.44 Mg/ha) was in Capsicum frutescens in control plots (T1). The highest fruit diameter (11.08 mm) and longest fruits (18.39 mm) were observed in Capsicum chinense cv. Big Sun plants supplied with 30 Mg/ha poultry manure. Capsicum frutescens fruits had the highest concentration of N (2.39%), P (0.42%) and K (3.06%). Essential oils analysis shows that there were 179 essential oils from pepper fruits of Capsicum chinense cv. Safi, with major essential oil been 3,4-dimethyl-benzaldehyde and main essential oils were alpha Terpinene and alpha Pinene based on their abundance. Fruits of Capsicum chinense cv. Big Sun had two-hundred and eight essential oils with major essential oil been 1,3,5-Trimethyl-1-H-pyrazol-4-amine and main essential oils were Caryophyllene oxide and Cymene, based on their abundance. Fruits of Capsicum frutescens had one-hundred and eighty-one essential oils with major essential oil been Limonene, and main essential oils were Carvacrol and D-limonene with respect to abundance. Results show that 20 Mg/ha poultry manure was the most cost effective in the production of pepper in Buea Cameroon.

Determining the Effects of Selected Organic Fertilizer on Growth and Yields of Tomato (Lycopersicon esculentum: Var. Rio Grande Tomatoes) in Mundri West County, Western Equatoria State, South Sudan

Worldwide, the demand for agro-organic foods that are healthy, nutritious, and environmentally friendly is increasing dramatically across all nations among consumers. Tomatoes being one of the dietary requirements in almost every meal is not exceptional and its availability in the market all year round is very important to farmers as well as consumers because it is highly demanded as a vegetable par excellence;which is either eaten raw in salads, cooked or processed into liquid ingredients. This study investigates the impact of chicken, goat, and cow manure treatments on tomato plant growth response to height, leaf length, and width, as well as fruit yield. The experimental field trials were conducted over two planting seasons in Mundri West County. It followed a Complete Randomized Design (CRD) approach, consisting of four blocks. Each block contained three treatments replicated four times and a control group. The data of measured parameters from all 16 plots were subjected to one-way Analysis of Variance (ANOVA) using the Gen Stat 14th Edition software. The findings indicate significant differences (P < 0.05) among all the different organic manure applications on tomato plant growth parameters compared to the control group. Chicken manure resulted in the tallest tomato plants (30.1 and 37.9 cm), longest leaves (9.9 and 10.4 cm), and widest leaves (2.1 and 2.5 cm) in both seasons respectively. The study showed plots treated with chicken manure had a highly significant impact (P < 0.05) on the prevalence of aphids (1.0) and white flies (1.4) with the lowest value compared to those with cow and goat manure applied. Additionally, chicken manure led to the highest yields (39.30 and 49.49 tons/ha) in both seasons. Based on these findings, it can be concluded that using chicken manure effectively improves the performance of Rio Grande Tomatoes, and thus, farmers are encouraged to utilize chicken manure to maximize their tomato yields.

A Study on Rice Growth and Soil Environments in Paddy Fields Using Different Organic and Chemical Fertilizers

Currently, the majority of paddy fields in Japan are grown using chemical fertilizers and synthetic chemical pesticides, since chemical fertilizers can provide the nutrients necessary for plant growth. However, there are concerns regarding the environmental impact of chemical fertilizer and pesticides production, such as reduction of soil microorganisms and water pollution due to the runoff of fertilizer components from the soil caused by excessive fertilizer application. In this study, we investigated the effects of the application of organic and chemical fertilizers on the plant growth of paddy fields, in addition to their effects on the chemical and biological properties of the soil. The panicle numbers of rough and brown rice, the 1000-grain weight of the rough and brown rice, and the percentages of ripened grains were significantly higher in paddy soils grown with organic fertilizers than in those grown with chemical fertilizers. In addition, the total carbon (TC) contents and pH values were significantly higher in the soils of paddy fields grown with organic fertilizers. Furthermore, the soils of paddy fields grown with organic fertilizers exhibited greater bacterial biomasses, N circulation activity, and P circulation activity than the soils of paddy fields grown using chemical fertilizers, although the differences were not significant. In this study, the difference in plant growth was appeared in fertilizer application such as organic and chemical fertilizers. It was indicated that the organic fertilizer and pesticide reduction management increased the soil bacterial biomass and activated the material cycle such as N circulation activity.

Interactions between lead(Ⅱ) ions and dissolved organic matter derived from organic fertilizers incubated in the field

This work was to study composition characteristics and the subsequent effect on the lead(Pb) binding properties of dissolved organic matter(DOM) derived from seaweed-based(SWOF) and chicken manure organic fertilizers(CMOF) during a one-year field incubation experiment using the excitation-emission matrix-parallel factor(EEM-PARAFAC) and twodimensional correlation spectroscopy(2DCOS) analysis. Results showed that high aromatic and hydrophobic fluorescent substances were enriched in CMOF-derived DOM and SWOFderived DOM and enhanced over time. And phenolic groups in the fulvic-like substances for SWOF-derived DOM and carboxyl groups in the humic-like substances for CMOF-derived DOM had the fastest responses over time, respectively. Moreover, both non-fluorescent polysaccharides and fluorescent humic-like substances or fulvic-like substances with aromatic(C=C) groups first participated in the binding process of Pb to SWOF-derived DOM on day 0 and 180 during the lead binding process. In contrast, humic-like substances associated with aromatic(C=C) and phenolic groups gave a faster response to Pb binding on day 360. Regarding CMOF-derived DOM, the fulvic-like substances associated with aromatic(C=C) and carboxylic groups displayed a faster response to Pb ions on day 0. Nonetheless,polysaccharides and humic-like associated with phenolic groups had a faster response on days 180 and 360. It is noteworthy that the polysaccharides, which participated in Pb binding to CMOF-derived DOM, posed a higher risk of Pb in the environment after 360 days. Therefore, these findings gave new insights into the long-term applications of commercial organic fertilizers for the amendment of soil.

Effects of Organic and Medium and Trace Element Fertilizers on Yield and Quality of Panax notoginseng

[Objectives]This study aimed to investigate the effects of organic and medium and trace element fertilizers(Ca,Zn,B)on yield and quality of Panax notoginseng to provide theoretical support for rational fertilization in cultivation of P.notoginseng.[Methods]Five fertilization treatments,control(CK),organic fertilizer(OM),zinc fertilizer(ZF),boron fertilizer(BF)and lime(LF),were designed.A two-consecutive-year field plot trail was conducted.The biological traits,yield and saponin content of P.notoginseng were determined.[Results]The application of organic fertilizer had no significant effect on the biological traits of P.notoginseng.Trace element fertilizers significantly increased the scape length of P.notoginseng.Among the treatments,ZF significantly increased the single flower weight but reduced the inflorescence diameter,while the effects of BF were opposite to those of ZF;LF significantly increased the stem thickness and reduced the plant height.All treatments significantly increased the seedling rate of three-year-old P.notoginseng,and the increase in the LF group(20.49%)was the largest,followed by those in the ZF(16.80%)and OM(16.40%)groups,and the increase in the BF group(13.08%)was the smallest.Although OM,ZF and BF treatments caused the root weight of individual plants to decrease,the final yield of each treatment was higher than that of the control group,and the increases in the BF and LF groups exceeded 17%(P<0.05).The total saponin outputs of all the treatments except OM were significantly higher than that of the control group.[Conclusions]Under the conditions of this test,the supplementation of organic and medium and trace element fertilizers on the basis of conventional fertilization will help to increase the yield of P.notoginseng.However,the reduction of the total saponin output of P.notoginseng caused by organic fertilizer cannot be ignored.

Effects of Organic Compound Fertilizers on Soil Water Infiltration Characteristics

In order to study the effects of organic compound fertilizers on soil water infiltration characteristics,an indoor one-dimensional soil column water infiltration test was carried out.Six soil sample treatment groups were set,namely marshy solonchak(control check,CK for short),bamboo charcoal-mixed marshy solonchak,Difuyuan-mixed marshy solonchak,salined flavo-aquic soil(CK),bamboo charcoal-mixed salined flavo-aquic soil and Difuyuan-mixed salined flavo-aquic soil.After 120 min of infiltration,compared with the CK groups of marshy solonchak and salined flavo-aquic soil,the cumulative infiltration volumes of the bamboo charcoal and difuyuan treatment groups increased by -18.78%,-3.93% and 25.77%,6.53%,respectively;and the displacement increased by -18.93%,1.64% and 22.6%,12.5%,respectively.The relationship between the wetting front displacement and time conformed to a linear function.The effects of organic compound fertilizers on the initial infiltration rates of marshy solonchak and salined flavo-aquic soil were significant,and the relationship between infiltration rate and time conformed to a power function.The vertical distribution of soil moisture under the application of organic compound fertilizers showed that the moisture content changed relatively small within 5 cm of the surface soil layer,then decreased slowly within 5-10 cm of the soil layer,and decreased drastically below 10 cm of soil layer;and relative to the CK group,the application of organic compound fertilizers was beneficial to increase the moisture content of salined flavo-aquic soil.The organic compound fertilizers reduced the water infiltration capacity of marshy solonchak and improved the water infiltration capacity of salined flavo-aquic soil.Compared with Difuyuan,bamboo charcoal reduced the water infiltration capacity of marshy solonchak better and improved the water permeability of salined flavo-aquic soil more significantly.

Effects of Combined Application of Biochar-based Organic Fertilizer and Reduced Nitrogen Fertilizer on Soil Enzyme Activity and Yield of Purple Cabbage(Brassica oleracea var.capita rubra)in Yuanmou County

[Objectives]In response to the issue of soil improvement in Yuanmou County,the effects of combined application of biochar-based organic fertilizer and reduced nitrogen fertilizer on soil nutrients,soil enzyme activity,and yield of purple cabbage(Brassica oleracea var.capita rubra)were investigated in the field base of Institute of Thermal Zone Ecological Agriculture,Yunnan Academy of Agricultural Sciences in Yuanmou County.[Methods]A total of 13 treatments were set up by applying biochar-based organic fertilizer at three levels of 15,30 and 45 t/hm^(2)(T_(1),T_(2),T_(3)),combined with top application of nitrogen fertilizer(urea)at four levels:375(N_1),300(N_(2)),225(N_(3))and 0 kg/hm^(2),with non-fertilizing treatment as control check(CK),in order to explore the optimal ratio for the combined application of biochar-based organic fertilizer with nitrogen fertilizer.[Results]The application of biochar-based organic fertilizer could significantly improve soil nutrients,enzyme activity,and purple cabbage yield.The improvement effect of combined application with nitrogen fertilizer was higher than that of single application of biochar-based organic fertilizer,and the improvement effect was enhanced with the application amount of biochar-based organic fertilizer increasing.The contents of organic matter and total nitrogen were the highest in treatment T_(3)N_(3),of which the values increased by 81.39%and 56.09%compared with the CK,respectively.The contents of soil hydrolyzable nitrogen,available phosphorus,and available potassium were all the highest under treatment T_(3)N_(2),with increases of 92.76%,171.01%and 235.50%,respectively.There was a significant positive correlation between the activity of soil catalase,urease,and sucrase and organic matter,total nitrogen,and available nutrients.The overall soil enzyme activity was relatively higher in treatment T_(3)N_(2).The yield of purple cabbage treated with biochar-based organic fertilizer combined with nitrogen fertilizer could reach 85750 kg/hm^(2),which was 94.78%higher than that treated with biochar-based organic fertilizer alone.Based on comprehensive analysis,the optimal combination ratio was 45 t/hm^(2)of biochar-based organic fertilizer and 300 kg/hm^(2)of urea(T_(3)N_(2)).[Conclusions]This study provides data support for the promotion of biochar-based organic fertilizers and reduced fertilizer in agricultural soil in the Dam area of Yuanmou County.

Determination of Egg Shell Structure and Mineral Composition Using SEM-EDS and Identification the Possibility to Produce Fertilizer

Throwing out egg shells without using them depreciates a vital source of calcium. As an egg shell contains twice the amount of calcium a person needs a day, it is considered as the richest source of calcium of natural origin. Egg shells have been traditionally and widely used in medicine, beauty science and food production for decades. In spite of that, there is a lack of new solutions that profoundly study mineral elements and beneficial components contained in egg shells for further usage as a product. Assuming we consume 2 eggs a day, the yearly consumption for a person is 730 eggs. As beneficial component, the egg shell structure and mineral elements, were determined in 3 types of samples (Mongolian eggs, Russian eggs, and iodized eggs) by SEM-EDS (Scanning electron microscopy with energy dispersive spectroscopy), a state-of-the-art research method. Consequently, it was established that egg shells consists of Ca (72.6% - 85.7%), Mg (2.7% - 4.5%), Si (0.3% - 0.6%), P (7.0% - 18.1%), S (0.5% - 2.0%), K (0.4% - 0.9%), I (2.6% - 3.0%), respectively. Additionally, the D3.8 × 120, 250, 500, and 1000 times zoomed images of shell structure (SEM) of each sample were examined, and the results were compared and evaluated. The reverse titration method examination has demonstrated that the calcium carbonate (CaCO3) content in egg shells is 91% - 92.5%, and pH is 8.41 - 8.75. Ultimately, fertilizer containing 97.7% Ca without chemical additives has been extracted by grounding the eggshells to 4.4 μm, then preparing the mixture at the rate of 20:0.5 (shell: mix, enriched with mandarin and lemon peel) and adjusting its pH to 7.5 - 8.0. Further research on the impact of fertilizer on growing process of indoor flowers has been commenced.

Isolation and Characterization of Beneficial Microorganisms in Organic, Semi-Organic and Conventional Fertilizer Treated Agricultural Field Soil and Comparison of Bacterial Richness

The effect of different farming systems on microbial communities in agricultural environment was investigated in the present study. Depending on the present farming trend, the microbial distribution in agricultural soils treated with organic, semi-organic and conventional fertilizers was analyzed. A total of 20 soil samples were collected from different types of agricultural fields of Bangladesh Agricultural Research Institute (BARI, Gazipur). Microorganisms playing beneficial roles in soil such as nitrogen fixation (e.g. Rhizobium sp., Azotobacter sp.), phosphate solubilization (e.g. Bacillus sp., Pseudomonas sp., Phosphobacteria) and auxin production (e.g. Pseudomonas sp., Serratia sp. and Bacillus sp.) were evaluated from each of the samples. The results revealed that agricultural fields treated with chemical fertilizers showed lower microbial count than that of organic fertilizer treated agricultural fields’ soil samples. In addition, organic fertilizers amended field soils have higher phytohormone (Auxin) activities, phosphate solubilization bacteria and other bacterial richness compared to chemical fertilizer applied field soil.