The effects of substrate mingling ratio(SMR)(1:1,1:2,1:3,3:1,and 2:1)and organic loading rate(OLR)(50-90 g total solids per liter per day)on anaerobic co-digestion performance and microbial characteristics were invest...The effects of substrate mingling ratio(SMR)(1:1,1:2,1:3,3:1,and 2:1)and organic loading rate(OLR)(50-90 g total solids per liter per day)on anaerobic co-digestion performance and microbial characteristics were investigated for pig manure(PM)and pretreated/untreated corn stover in batch and semicontinuous anaerobic digestion(AD)system.The results showed that SMR and pretreatment affected co-digestion performance.The maximum cumulative methane yield of 428.5 ml·g^(-1)(based on volatile solids(VS))was obtained for PCP13,which was 35.7%and 40.0%higher than that of CSU and PM.In the first 5 days,the maximum methane yield improvement rate was 378.1%for PCP13.The daily methane yield per gram VS of PCP13 was 11.4%-18.5%higher than that of PC_(U)13.Clostridium_sensu_stricto_1,DMER64,and Bacteroides and Methanosaeta,Methanobacterium,and Methanospirillum had higher relative abundance at the genus level.Therefore,SMR and OLR are important factor affecting the AD process,and OLR can affect methane production through volatile fatty acids.展开更多
The performance and rnicrobial community structure in an upflow anaerobic sludge blanket reactor (UASB) treating sugar refinery wastewater were investigated. The chemical oxygen demand (COD) removal reached above ...The performance and rnicrobial community structure in an upflow anaerobic sludge blanket reactor (UASB) treating sugar refinery wastewater were investigated. The chemical oxygen demand (COD) removal reached above 92.0% at organic loading rates (OLRs) of 12.0-54.0 kgCOD/(m^3· d). The volatile lhtty acids (VFAs) in effluent were increased to 451.1 mg/L from 147.9 mg/L and the specific methane production rate improved by 1.2 2.2-1bid as the OLR increased. The evolution of microbial comnmnities in anaerobic sludge at three different OLRs was investigated using pyrosequencing. Operational taxonomic units (OTUs) at a 3% distance were 353,337 and 233 for OLRI2, OLR36 and OLR54, respectively. When the OLR was increased to 54.0 kgCOD/(m^3· d) from 12.0 kgCOD/ (m^3· d) by stepwise, the microbial community structure were changed significantly. Five genera (Bacteroides, Trichococcus, Cho,seobacterium, Longilinea and Aerococcus) were the dominant fermentative bacteria at the OLR 12-0 kgCOD/(m^3· d). However, the sample of OLR36 was dominated by Lacmcoccus, Trichococcus, Anaer-arcus and Veillonella. At the last stage (OLR = 54.0 kgCOD/ (m^3· d), the diversity and percentage of femlentative bacteria were markedly increased. Apart from fermentative bacteria, an obvious shift was observed in hydrogen-producing acetogens and non- acetotrophic methanogens as OLR increased. Svntrophohacter, Geobacter and Methanomethylovor- ans were the dominant hydrogen-producing acetogens and methylotrophic methanogens in the samples of OLRI2 and OLR36. When the OLR was increased to 54.0 kgCOD/(m^3· d), the mare hydrogen-producing acetogens and hydrogenotrophic methanogens were substituted with Destd/bvi- brio and Methanospillum. However, the composition of acetotrophic methanogens (Methanosaeta) was relatively stable during the whole operation period of the UASB reactor.展开更多
treatability of synthetic sago wastewater was investigated in a laboratory anaerobic tapered fluidized bed reactor (ATFBR) with a mesoporous granular activated carbon (GAC) as a support material. The experimental ...treatability of synthetic sago wastewater was investigated in a laboratory anaerobic tapered fluidized bed reactor (ATFBR) with a mesoporous granular activated carbon (GAC) as a support material. The experimental protocol was defined to examine the effect of the maximum organic loading rate (OLR), hydraulic retention time (HRT), the efficiency of the reactor and to report on its steady- state performance. The reactor was subjected to a steady-state operation over a range of OLR up to 85.44 kg COD/(m^3·d). The COD removal efficiency was found to be 92% in the reactor while the biogas produced in the digester reached 25.38 m^3/(m^3·d) of the reactor. With the increase of OLR from 83.7 kg COD/(m^3·d), the COD removal efficiency decreased. Also an artificial neural network (ANN) model using multilayer perceptron (MLP) has been developed for a system of two input variable and five output dependent variables. For the training of the input-output data, the experimental values obtained have been used. The output parameters predicted have been found to be much closer to the corresponding experimental ones and the model was validated for 30% of the untrained data. The mean square error (MSE) was found to be only 0.0146.展开更多
The present study deals with the performance evaluation of the UASB reactor under varied organic loading rate(OLR) for the treatment of paper mill wastewater. The sludge granulation process started after 120 days from...The present study deals with the performance evaluation of the UASB reactor under varied organic loading rate(OLR) for the treatment of paper mill wastewater. The sludge granulation process started after 120 days from the start-up period. Sludge granules size was found to be 0 8 mm at OLR of 1 72 kgCOD/(m 3·d), which reached maximum size of about 1 0 to 1 2 mm at OLR of 2 1 kgCOD/(m 3·d). At the end of initial OLR of 1 0 kgCOD/(m 3·d) the VSS concentration was 12 86 gVSS/L, which got increased to 38 05 gVSS/L at the end of an OLR 2 1 kgCOD/(m 3·d). Most of the times VFA recorded were well within the limit of VFA reported in anaerobic fermentation process. Many times the pH observed was between 6 5 and 7 8, which is more favorable for any anaerobic process. It is also found that pH within the reactor increases along with the height of reactor. The total maximum biogas production was found to be 0 40 L/gCOD removals at OLR of 2 1 kgCOD/(m 3·d) and the maximum BOD removal at this stage was observed to be 90%.展开更多
The investigation of the biodegradability and methane potential of bacterial pre-treated miscanthus sinensis has been carried out.One percent solution of Bacta-sile:A silage promoter was used to pre-treat miscanthus s...The investigation of the biodegradability and methane potential of bacterial pre-treated miscanthus sinensis has been carried out.One percent solution of Bacta-sile:A silage promoter was used to pre-treat miscanthus sinensis at 25℃.The anaerobic digestion experiments were carried out at 25℃ and 35℃ in batch experiments.The organic loading rates(OLR)varied between 1.25 g and 7 g in different batch reactors.The results showed that the highest methane concentration was 57% from digester 1 while the lowest methane produced was 38% from digester 3.The low methane production from digester 3 was attributed to temperature changes and poor organic loading rate.Bacterial pretreatment aided biodegradation of miscanthus at 25℃.Operating temperature of 25℃ had a great effect on digestion experiments resulting to longer required Hydraulic Retention Time(HRT).展开更多
Aerobic granules, pre-cultivated at the organic loading rate (OLR) of 3.0 kg COD/(m3 ·day), were used to treat low-strength wastewater in two sequencing batch reactors at low OLRs of 1.2 and 0.6 kg COD/(m3 &...Aerobic granules, pre-cultivated at the organic loading rate (OLR) of 3.0 kg COD/(m3 ·day), were used to treat low-strength wastewater in two sequencing batch reactors at low OLRs of 1.2 and 0.6 kg COD/(m3 ·day), respectively. Reactor performance, evolution of granule morphology, structure and microbial community at low OLRs under long-term operation (130 days) were investigated. Results showed that low OLRs did not cause significant damage to granule structure as a dominant granule morphology with size over 540 μm was maintained throughout the operation. Aerobic granules at sizes of about 750 μm were finally obtained at the low OLRs. The granule reactors operated at low OLRs demonstrated effective COD and ammonia removals (above 90%), smaller granule sizes and less biomass. The contents of extracellular polymeric substances in the granules were decreased while the ratios of exopolysaccharide/exoprotein were increased (above 1.0). The granules cultivated at the low OLRs showed a smoother surface and more compact structure than the seeded granules. A significant shift in microbial community was observed but the microbial diversity remained relatively stable. Confocal Laser Scanning Microscopy observation showed that the live cells were spread throughout the whole granule, while the dead cells were mainly concentrated in the outer layer of the granule, and the proteins, polysaccharides and lipids were mainly located in the central regime of the granule. In conclusion, granules cultivated at high OLRs show potential for treating low-strength organic wastewater steadily under long-term operation.展开更多
Parallel pilot-scale anaerobic digestion systems were conducted to evaluate the influence of system temperatures(30℃and 35℃)on digestion performance,greenhouse gas control and economic efficiency.Biogas productions(...Parallel pilot-scale anaerobic digestion systems were conducted to evaluate the influence of system temperatures(30℃and 35℃)on digestion performance,greenhouse gas control and economic efficiency.Biogas productions(6.64-12.96 m3/d)and methane yields(0.46-0.61 m3/kg VS)of 35℃digestion system were significantly higher than those of 30℃digestion system with the organic loading rate(OLR)of 2.0-4.5 kg VS/m3·d.Two regression equations of methane yields with increasing OLRs were fitted at 30℃and 35℃to predict the methane production of practical food waste(FW)digestion plants.By analyzing process stability,the optimal operating OLRs of 35℃digestion system(4.0 kg VS/m3·d)was found to be higher than that of 30℃digestion system(3.0 kg VS/m3·d),indicating that the 35℃digestion system had better processing capacity.The greenhouse gas emission under corresponding optimal operating OLR of 35℃digestion system was also calculated to be better than that of 30℃digestion system.Even the system temperature of 30℃was found to be more suitable for the digestion where OLR was less than 3.0 kg VS/m3·d,a higher operational temperature of 35℃was still a better choice for conventional high-solid digestion.展开更多
This paper describes the feasibility of fresh leachate treatment by an upflow blanket filter(UBF).Through dilution and partial effluent recycling,the organic loading rates increased from 0.51 to 14.56 kg COD/(m^(3)...This paper describes the feasibility of fresh leachate treatment by an upflow blanket filter(UBF).Through dilution and partial effluent recycling,the organic loading rates increased from 0.51 to 14.56 kg COD/(m^(3)·d),meanwhile the corresponding hydraulic retention time decreased from 9.0 to 3.6 d.The reactor was able to achieve steady-state within 80 d.Based on the distribution of COD fluxes in the process,it was concluded that anabolism was the main pathway of COD removal in the initial phase(1–33 d),accounting for 57%–85%of total COD removed.As the anaerobic consortium of bacteria reached steady-state(after 70–86 d),the majority of COD removed was transformed into methane,because the specific methane yield was close to the theoretical value(0.36 L CH4/(g CODdeg)).展开更多
基金the fund supports from the Fundamental Research Funds for the Central Universities(JD2326).
文摘The effects of substrate mingling ratio(SMR)(1:1,1:2,1:3,3:1,and 2:1)and organic loading rate(OLR)(50-90 g total solids per liter per day)on anaerobic co-digestion performance and microbial characteristics were investigated for pig manure(PM)and pretreated/untreated corn stover in batch and semicontinuous anaerobic digestion(AD)system.The results showed that SMR and pretreatment affected co-digestion performance.The maximum cumulative methane yield of 428.5 ml·g^(-1)(based on volatile solids(VS))was obtained for PCP13,which was 35.7%and 40.0%higher than that of CSU and PM.In the first 5 days,the maximum methane yield improvement rate was 378.1%for PCP13.The daily methane yield per gram VS of PCP13 was 11.4%-18.5%higher than that of PC_(U)13.Clostridium_sensu_stricto_1,DMER64,and Bacteroides and Methanosaeta,Methanobacterium,and Methanospirillum had higher relative abundance at the genus level.Therefore,SMR and OLR are important factor affecting the AD process,and OLR can affect methane production through volatile fatty acids.
基金This work was supported by the National Natural Science Foundation of China (Nos. 51508316 and 51708341 ), Open Project of State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (No. QA201523), HIT Environment and Ecology Innovation Special Funds (No. HSCJ201614). Research Project for Young Sanjin Scholarship of Shanxi, Program for the Outstanding Innovative Team of Higher Learning Institutions of Shanxi, and Research Fund of Tianjin Key Laboratory of Aquatic Science and Technology (No. TJKLAST- ZD-2016- 05).
文摘The performance and rnicrobial community structure in an upflow anaerobic sludge blanket reactor (UASB) treating sugar refinery wastewater were investigated. The chemical oxygen demand (COD) removal reached above 92.0% at organic loading rates (OLRs) of 12.0-54.0 kgCOD/(m^3· d). The volatile lhtty acids (VFAs) in effluent were increased to 451.1 mg/L from 147.9 mg/L and the specific methane production rate improved by 1.2 2.2-1bid as the OLR increased. The evolution of microbial comnmnities in anaerobic sludge at three different OLRs was investigated using pyrosequencing. Operational taxonomic units (OTUs) at a 3% distance were 353,337 and 233 for OLRI2, OLR36 and OLR54, respectively. When the OLR was increased to 54.0 kgCOD/(m^3· d) from 12.0 kgCOD/ (m^3· d) by stepwise, the microbial community structure were changed significantly. Five genera (Bacteroides, Trichococcus, Cho,seobacterium, Longilinea and Aerococcus) were the dominant fermentative bacteria at the OLR 12-0 kgCOD/(m^3· d). However, the sample of OLR36 was dominated by Lacmcoccus, Trichococcus, Anaer-arcus and Veillonella. At the last stage (OLR = 54.0 kgCOD/ (m^3· d), the diversity and percentage of femlentative bacteria were markedly increased. Apart from fermentative bacteria, an obvious shift was observed in hydrogen-producing acetogens and non- acetotrophic methanogens as OLR increased. Svntrophohacter, Geobacter and Methanomethylovor- ans were the dominant hydrogen-producing acetogens and methylotrophic methanogens in the samples of OLRI2 and OLR36. When the OLR was increased to 54.0 kgCOD/(m^3· d), the mare hydrogen-producing acetogens and hydrogenotrophic methanogens were substituted with Destd/bvi- brio and Methanospillum. However, the composition of acetotrophic methanogens (Methanosaeta) was relatively stable during the whole operation period of the UASB reactor.
文摘treatability of synthetic sago wastewater was investigated in a laboratory anaerobic tapered fluidized bed reactor (ATFBR) with a mesoporous granular activated carbon (GAC) as a support material. The experimental protocol was defined to examine the effect of the maximum organic loading rate (OLR), hydraulic retention time (HRT), the efficiency of the reactor and to report on its steady- state performance. The reactor was subjected to a steady-state operation over a range of OLR up to 85.44 kg COD/(m^3·d). The COD removal efficiency was found to be 92% in the reactor while the biogas produced in the digester reached 25.38 m^3/(m^3·d) of the reactor. With the increase of OLR from 83.7 kg COD/(m^3·d), the COD removal efficiency decreased. Also an artificial neural network (ANN) model using multilayer perceptron (MLP) has been developed for a system of two input variable and five output dependent variables. For the training of the input-output data, the experimental values obtained have been used. The output parameters predicted have been found to be much closer to the corresponding experimental ones and the model was validated for 30% of the untrained data. The mean square error (MSE) was found to be only 0.0146.
文摘The present study deals with the performance evaluation of the UASB reactor under varied organic loading rate(OLR) for the treatment of paper mill wastewater. The sludge granulation process started after 120 days from the start-up period. Sludge granules size was found to be 0 8 mm at OLR of 1 72 kgCOD/(m 3·d), which reached maximum size of about 1 0 to 1 2 mm at OLR of 2 1 kgCOD/(m 3·d). At the end of initial OLR of 1 0 kgCOD/(m 3·d) the VSS concentration was 12 86 gVSS/L, which got increased to 38 05 gVSS/L at the end of an OLR 2 1 kgCOD/(m 3·d). Most of the times VFA recorded were well within the limit of VFA reported in anaerobic fermentation process. Many times the pH observed was between 6 5 and 7 8, which is more favorable for any anaerobic process. It is also found that pH within the reactor increases along with the height of reactor. The total maximum biogas production was found to be 0 40 L/gCOD removals at OLR of 2 1 kgCOD/(m 3·d) and the maximum BOD removal at this stage was observed to be 90%.
文摘The investigation of the biodegradability and methane potential of bacterial pre-treated miscanthus sinensis has been carried out.One percent solution of Bacta-sile:A silage promoter was used to pre-treat miscanthus sinensis at 25℃.The anaerobic digestion experiments were carried out at 25℃ and 35℃ in batch experiments.The organic loading rates(OLR)varied between 1.25 g and 7 g in different batch reactors.The results showed that the highest methane concentration was 57% from digester 1 while the lowest methane produced was 38% from digester 3.The low methane production from digester 3 was attributed to temperature changes and poor organic loading rate.Bacterial pretreatment aided biodegradation of miscanthus at 25℃.Operating temperature of 25℃ had a great effect on digestion experiments resulting to longer required Hydraulic Retention Time(HRT).
基金supported by the National Natural Science Foundation of China (No. 21077012, 51178049)
文摘Aerobic granules, pre-cultivated at the organic loading rate (OLR) of 3.0 kg COD/(m3 ·day), were used to treat low-strength wastewater in two sequencing batch reactors at low OLRs of 1.2 and 0.6 kg COD/(m3 ·day), respectively. Reactor performance, evolution of granule morphology, structure and microbial community at low OLRs under long-term operation (130 days) were investigated. Results showed that low OLRs did not cause significant damage to granule structure as a dominant granule morphology with size over 540 μm was maintained throughout the operation. Aerobic granules at sizes of about 750 μm were finally obtained at the low OLRs. The granule reactors operated at low OLRs demonstrated effective COD and ammonia removals (above 90%), smaller granule sizes and less biomass. The contents of extracellular polymeric substances in the granules were decreased while the ratios of exopolysaccharide/exoprotein were increased (above 1.0). The granules cultivated at the low OLRs showed a smoother surface and more compact structure than the seeded granules. A significant shift in microbial community was observed but the microbial diversity remained relatively stable. Confocal Laser Scanning Microscopy observation showed that the live cells were spread throughout the whole granule, while the dead cells were mainly concentrated in the outer layer of the granule, and the proteins, polysaccharides and lipids were mainly located in the central regime of the granule. In conclusion, granules cultivated at high OLRs show potential for treating low-strength organic wastewater steadily under long-term operation.
基金We acknowledge that this work was financially support by University Doctorial Foundation(No.20120010110004)Beijing Natural Science Foundation(No.8142030).
文摘Parallel pilot-scale anaerobic digestion systems were conducted to evaluate the influence of system temperatures(30℃and 35℃)on digestion performance,greenhouse gas control and economic efficiency.Biogas productions(6.64-12.96 m3/d)and methane yields(0.46-0.61 m3/kg VS)of 35℃digestion system were significantly higher than those of 30℃digestion system with the organic loading rate(OLR)of 2.0-4.5 kg VS/m3·d.Two regression equations of methane yields with increasing OLRs were fitted at 30℃and 35℃to predict the methane production of practical food waste(FW)digestion plants.By analyzing process stability,the optimal operating OLRs of 35℃digestion system(4.0 kg VS/m3·d)was found to be higher than that of 30℃digestion system(3.0 kg VS/m3·d),indicating that the 35℃digestion system had better processing capacity.The greenhouse gas emission under corresponding optimal operating OLR of 35℃digestion system was also calculated to be better than that of 30℃digestion system.Even the system temperature of 30℃was found to be more suitable for the digestion where OLR was less than 3.0 kg VS/m3·d,a higher operational temperature of 35℃was still a better choice for conventional high-solid digestion.
基金the National High-Tech Research and Development Program(863 Program)of China(Grant No.2008AA062101)the Key Project of Chinese Ministry of Education(No.107122)。
文摘This paper describes the feasibility of fresh leachate treatment by an upflow blanket filter(UBF).Through dilution and partial effluent recycling,the organic loading rates increased from 0.51 to 14.56 kg COD/(m^(3)·d),meanwhile the corresponding hydraulic retention time decreased from 9.0 to 3.6 d.The reactor was able to achieve steady-state within 80 d.Based on the distribution of COD fluxes in the process,it was concluded that anabolism was the main pathway of COD removal in the initial phase(1–33 d),accounting for 57%–85%of total COD removed.As the anaerobic consortium of bacteria reached steady-state(after 70–86 d),the majority of COD removed was transformed into methane,because the specific methane yield was close to the theoretical value(0.36 L CH4/(g CODdeg)).