An efficient procedure was described for the transformation of the monocotyledonous oriental hybrid lily, Lilium cv. Siberia. by Agrobacterium-mediated genetic transformation via leaves regeneration, The leaves of lea...An efficient procedure was described for the transformation of the monocotyledonous oriental hybrid lily, Lilium cv. Siberia. by Agrobacterium-mediated genetic transformation via leaves regeneration, The leaves of leaflets which derived from bulbs were sliced into 1.0 cm long and were co-cultivated with A. tumefaciens strain LBA4404/pB2AE12, which harbored a vector carrying the neomycin phosphotransferase, DREB2A genes in the T-DNA region. The suitable genetic transformation condition was determined as follows： the bacterial concentration reached 0.5-0.6 （OD600）, 15 min infection time, 20 mg.L^-1 acetosyingone, and 10.6 mmol.L^-1 NH4NO3 medium was used for co-cultivation 3 days, delayed 7 days for selecting by 30 mg.L^-1 kanamycin containing regeneration medium. Efficient shoot regeneration was observed on MS medium supplemented with 1.0 mg.L^-1 naphthaleneacetic acid, 0.5 mg.L^-1 benzyladenine and 0.1 mg.L^-1 Kinetin after about 6 weeks culture. The presence ofDREB2A gene in the genomic DNA of regenerated plants was detected by means of PCR analysis.展开更多
The present study was to test the hypothesis that the plant growth retardants chlorocholine chloride(CCC) and paclobutrazol(PBZ)could improve the carbohydrate accumulation in lily bulbs by enhancing photosynthetic cap...The present study was to test the hypothesis that the plant growth retardants chlorocholine chloride(CCC) and paclobutrazol(PBZ)could improve the carbohydrate accumulation in lily bulbs by enhancing photosynthetic capacity and changing endogenous hormones.Plants of Lilium Oriental hybrids'Sorbonne'were treated with a foliar spray of CCC or PBZ(both at 300 mg/L)solution,at six weeks after planting(6 WAP).The morphological parameters,endogenous hormone contents(gibberellic acid(GA),abscisic acid(ABA),and indole-3-acetic acid(IAA)),and carbohydrate contents were measured from 6 to 18 WAP,at 2-week intervals.The results showed that CCC increased the biomass of leaves and stems which might produce more photoassimilates available for transportation and utilization.However,PBZ treatment suppressed vegetative growth and favored photoassimilate transportation into bulbs.A slight delay of bud and anthesis formation was observed in both treated plants.CCC and PBZ treatments substantially enhanced the sucrose contents in leaves probably due to the increase of chlorophyll contents.Treatment with CCC or PBZ decreased GA but increased IAA contents in lily bulbs which might stimulate starch accumulation and formation of new scales.Our experiment suggested that CCC or PBZ treatment is an effective method to promote carbohydrate accumulation in lily bulbs.展开更多
To understand the ecophysiological adapta-tion of Lilium"Oriental Hybrids",which are grown for their commercial bulbs,the gas exchange,leaf N and chlorophyll content of the three varieties were investigated ...To understand the ecophysiological adapta-tion of Lilium"Oriental Hybrids",which are grown for their commercial bulbs,the gas exchange,leaf N and chlorophyll content of the three varieties were investigated in the central areas of the Yunnan Province.Among the three varieties,light-saturated photosynthetic rate at ambient CO_(2)(Amax)of Tiber was the highest,while that of Siberia was the lowest.The difference in the Amax was related to the carboxylation efficiency(CE),leaf mass per unit area and leaf N content per mass,which indicated that their photosynthetic capacity was influenced by the activity and/or the quantity of Rubisco.The three vari-eties had lower photosynthetic saturation points and pho-tosynthetic compensation points,but the photosynthetic rates were not decreased up to 2000 μmol·m^(-2)·s^(-1) of the light intensity.This indicates that the three varieties had broad adaptability to light intensity.There were signifi-cant differences in the photosyn thetic optimum temper-ature among the three varieties.Siberia had the highest photosynthetic optimum temperature(25.5℃-34.9℃),and is likely to grow well in warm areas.Sorbonne had the lowest photosynthetic optimum temperature(19.3℃-25.6℃),and its growth is favored in cool areas.Tiber can maintain a high photosynthetic rate within a wide range of temperature.Therefore,Tiber is the most suitable variety for the climate in the central areas of the Yunn an Province,China.展开更多
基金Supported by Postdoctoral Fund of Settling Down in Heilongjiang Province (LBH-Z08259)Program for Dr. Research Fund of Northeast Agricultural University
文摘An efficient procedure was described for the transformation of the monocotyledonous oriental hybrid lily, Lilium cv. Siberia. by Agrobacterium-mediated genetic transformation via leaves regeneration, The leaves of leaflets which derived from bulbs were sliced into 1.0 cm long and were co-cultivated with A. tumefaciens strain LBA4404/pB2AE12, which harbored a vector carrying the neomycin phosphotransferase, DREB2A genes in the T-DNA region. The suitable genetic transformation condition was determined as follows： the bacterial concentration reached 0.5-0.6 （OD600）, 15 min infection time, 20 mg.L^-1 acetosyingone, and 10.6 mmol.L^-1 NH4NO3 medium was used for co-cultivation 3 days, delayed 7 days for selecting by 30 mg.L^-1 kanamycin containing regeneration medium. Efficient shoot regeneration was observed on MS medium supplemented with 1.0 mg.L^-1 naphthaleneacetic acid, 0.5 mg.L^-1 benzyladenine and 0.1 mg.L^-1 Kinetin after about 6 weeks culture. The presence ofDREB2A gene in the genomic DNA of regenerated plants was detected by means of PCR analysis.
文摘The present study was to test the hypothesis that the plant growth retardants chlorocholine chloride(CCC) and paclobutrazol(PBZ)could improve the carbohydrate accumulation in lily bulbs by enhancing photosynthetic capacity and changing endogenous hormones.Plants of Lilium Oriental hybrids'Sorbonne'were treated with a foliar spray of CCC or PBZ(both at 300 mg/L)solution,at six weeks after planting(6 WAP).The morphological parameters,endogenous hormone contents(gibberellic acid(GA),abscisic acid(ABA),and indole-3-acetic acid(IAA)),and carbohydrate contents were measured from 6 to 18 WAP,at 2-week intervals.The results showed that CCC increased the biomass of leaves and stems which might produce more photoassimilates available for transportation and utilization.However,PBZ treatment suppressed vegetative growth and favored photoassimilate transportation into bulbs.A slight delay of bud and anthesis formation was observed in both treated plants.CCC and PBZ treatments substantially enhanced the sucrose contents in leaves probably due to the increase of chlorophyll contents.Treatment with CCC or PBZ decreased GA but increased IAA contents in lily bulbs which might stimulate starch accumulation and formation of new scales.Our experiment suggested that CCC or PBZ treatment is an effective method to promote carbohydrate accumulation in lily bulbs.
文摘To understand the ecophysiological adapta-tion of Lilium"Oriental Hybrids",which are grown for their commercial bulbs,the gas exchange,leaf N and chlorophyll content of the three varieties were investigated in the central areas of the Yunnan Province.Among the three varieties,light-saturated photosynthetic rate at ambient CO_(2)(Amax)of Tiber was the highest,while that of Siberia was the lowest.The difference in the Amax was related to the carboxylation efficiency(CE),leaf mass per unit area and leaf N content per mass,which indicated that their photosynthetic capacity was influenced by the activity and/or the quantity of Rubisco.The three vari-eties had lower photosynthetic saturation points and pho-tosynthetic compensation points,but the photosynthetic rates were not decreased up to 2000 μmol·m^(-2)·s^(-1) of the light intensity.This indicates that the three varieties had broad adaptability to light intensity.There were signifi-cant differences in the photosyn thetic optimum temper-ature among the three varieties.Siberia had the highest photosynthetic optimum temperature(25.5℃-34.9℃),and is likely to grow well in warm areas.Sorbonne had the lowest photosynthetic optimum temperature(19.3℃-25.6℃),and its growth is favored in cool areas.Tiber can maintain a high photosynthetic rate within a wide range of temperature.Therefore,Tiber is the most suitable variety for the climate in the central areas of the Yunn an Province,China.
