Terahertz time-domain spectroscopy(THz-TDS)is an effective nondestructive and noninvasive tool for investigating sulfur-containing pigments.Combined with Raman spectroscopy and vibrational mode analysis,it is signific...Terahertz time-domain spectroscopy(THz-TDS)is an effective nondestructive and noninvasive tool for investigating sulfur-containing pigments.Combined with Raman spectroscopy and vibrational mode analysis,it is significant for artifact identification and conservation.In this work,the terahertz absorption spectra of pararealgar(As_(4)S_(4))and orpiment(As_(2)S_(3))samples mixed with polytetrafluoroethylene(PTFE)are characterized in a range from 0.2 THz to 2.2 THz,and their distinctive peaks are observed,respectively.Meanwhile,qualitative analysis is also implemented by using Raman spectroscopy as a complementary technique.The lattice vibrations are simulated by using solid-state density functional theory(ss-DFT),illustrating different characteristic absorption peaks for specific crystalline structures and dynamic properties.This work provides a reliable database of sulfur-containing pigments for using the THz technology to actually analyze and diagnose cultural relics.展开更多
China's realgar/orpiment deposits may be classified into three types, the stratabound, hot-water sedimentary and hydrothermal, according to their mineralizing processes, geological occurrences, tectonic and geoche...China's realgar/orpiment deposits may be classified into three types, the stratabound, hot-water sedimentary and hydrothermal, according to their mineralizing processes, geological occurrences, tectonic and geochemical features. The three types may be further distinguished into seven subtypes, namely, the Xiaguan, Shuiluo, Jiepaiyu, Songpan, Shixia, Wangzhuang and Ninghshan ones. On this basis three minerogenic models are established, and based on studies of their geochemistry and minerogenic mechanisms the prerequisites for prospecting for these types of deposits are given in the paper.展开更多
Objective :To investigate PML. gene and protein expression and localization in leukemia cell lines. Methods Cell morphology was assayed by Wright’s stain and fluorescence stain, and PML mRNA expression by RT-PCR, PML...Objective :To investigate PML. gene and protein expression and localization in leukemia cell lines. Methods Cell morphology was assayed by Wright’s stain and fluorescence stain, and PML mRNA expression by RT-PCR, PML protein localization by immuno-fluorescence. Results NB4 and HL-60 cells differentiated morphologically after treatment with anti-retinoic acid (ATRA ) while K562 cells did not differentiate. Typical apoptosis was found in each cell line after treatment with red orpiment. Immuno-fluorescence analysis showed, after treatment with ATRA, the fusion protein disappeared in NB4 cells and the PML protein relocated, while HL-60 and K562 cells had no difference from control cells. After treatment with red orpiment, the fusion protein disappeared in NB4 cells, then degraded, which was also seen in HL-60 cells and K562 cells. The expression of PML mRNA was not changed in all three cell lines after treatment with ATRA or red orpiment. Conclusion PML plays the role of differentiation and apoptosis inducer in leukemia cells at the translational level. PML in POD plays the role of apoptosis inducer and the growth control of leukemia cells.展开更多
Objective To investigate the effects of red orpiment on cell morphology, expression of promyelocytic leukemia (PML) mRNA and its protein localization in NB4 and HL-60 cell lines.Methods Cell morphology was assayed b...Objective To investigate the effects of red orpiment on cell morphology, expression of promyelocytic leukemia (PML) mRNA and its protein localization in NB4 and HL-60 cell lines.Methods Cell morphology was assayed by Wright's staining and fluorescence staining, while PML mRNA expression was determined by RT-PCR. PML protein localization by evaluated by immunofluorescence staining. Results The typical apoptosis was found in NB4 and HL-60 cells after treatment with red orpiment. The fusion protein was no longer observed in NB4 cells, PML protein was relocated, and then degraded. In HL-60 cells, PML protein underwent a similar progress. The expression of promyelocytic leukemia (PML) mRNA was not changed in the treated cells.Conclusion Red orpiment inhibits the proliferation of leukemia cells by inducing them to undergo apoptosis.展开更多
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61805129 and 11874245)the National Key Research and Development Program of China(Grant No.2021YFB3200100)the Yungang Special Fund of Shanxi Datong University,China(Grant No.2020YGZX005)。
文摘Terahertz time-domain spectroscopy(THz-TDS)is an effective nondestructive and noninvasive tool for investigating sulfur-containing pigments.Combined with Raman spectroscopy and vibrational mode analysis,it is significant for artifact identification and conservation.In this work,the terahertz absorption spectra of pararealgar(As_(4)S_(4))and orpiment(As_(2)S_(3))samples mixed with polytetrafluoroethylene(PTFE)are characterized in a range from 0.2 THz to 2.2 THz,and their distinctive peaks are observed,respectively.Meanwhile,qualitative analysis is also implemented by using Raman spectroscopy as a complementary technique.The lattice vibrations are simulated by using solid-state density functional theory(ss-DFT),illustrating different characteristic absorption peaks for specific crystalline structures and dynamic properties.This work provides a reliable database of sulfur-containing pigments for using the THz technology to actually analyze and diagnose cultural relics.
文摘China's realgar/orpiment deposits may be classified into three types, the stratabound, hot-water sedimentary and hydrothermal, according to their mineralizing processes, geological occurrences, tectonic and geochemical features. The three types may be further distinguished into seven subtypes, namely, the Xiaguan, Shuiluo, Jiepaiyu, Songpan, Shixia, Wangzhuang and Ninghshan ones. On this basis three minerogenic models are established, and based on studies of their geochemistry and minerogenic mechanisms the prerequisites for prospecting for these types of deposits are given in the paper.
基金Partially supported by the National Natural Science Foundation of China(36970328)
文摘Objective :To investigate PML. gene and protein expression and localization in leukemia cell lines. Methods Cell morphology was assayed by Wright’s stain and fluorescence stain, and PML mRNA expression by RT-PCR, PML protein localization by immuno-fluorescence. Results NB4 and HL-60 cells differentiated morphologically after treatment with anti-retinoic acid (ATRA ) while K562 cells did not differentiate. Typical apoptosis was found in each cell line after treatment with red orpiment. Immuno-fluorescence analysis showed, after treatment with ATRA, the fusion protein disappeared in NB4 cells and the PML protein relocated, while HL-60 and K562 cells had no difference from control cells. After treatment with red orpiment, the fusion protein disappeared in NB4 cells, then degraded, which was also seen in HL-60 cells and K562 cells. The expression of PML mRNA was not changed in all three cell lines after treatment with ATRA or red orpiment. Conclusion PML plays the role of differentiation and apoptosis inducer in leukemia cells at the translational level. PML in POD plays the role of apoptosis inducer and the growth control of leukemia cells.
基金ThisstudywaspartiallysupportedbytheNationalNaturalScienceFoundationofChina (No 36 970 32 8)
文摘Objective To investigate the effects of red orpiment on cell morphology, expression of promyelocytic leukemia (PML) mRNA and its protein localization in NB4 and HL-60 cell lines.Methods Cell morphology was assayed by Wright's staining and fluorescence staining, while PML mRNA expression was determined by RT-PCR. PML protein localization by evaluated by immunofluorescence staining. Results The typical apoptosis was found in NB4 and HL-60 cells after treatment with red orpiment. The fusion protein was no longer observed in NB4 cells, PML protein was relocated, and then degraded. In HL-60 cells, PML protein underwent a similar progress. The expression of promyelocytic leukemia (PML) mRNA was not changed in the treated cells.Conclusion Red orpiment inhibits the proliferation of leukemia cells by inducing them to undergo apoptosis.
