Objective:To investigate effect of orthodontic force on inflammatory periodontal tissue remodeling and expression of IL-6 and IL-8 in rats.Methods:Eighty SD rats were randomly divided into 4 groups,blank control group...Objective:To investigate effect of orthodontic force on inflammatory periodontal tissue remodeling and expression of IL-6 and IL-8 in rats.Methods:Eighty SD rats were randomly divided into 4 groups,blank control group(group A)with 5 rats,treatment normal group(group B)with 25 rats,inflammation control group(group(group C)with 25 rats,inflammation treatment group(group D)with 25 rats.Immunohistochemistry and histomorphometric analysis was performed to measure the expression of IL-6,IL-8 and the firet molar to the recent movement in the distance.Results:The expression of IL-8 reached a maximum on day 5 and declined thereafter in group B;the expression of IL-6 reached a maximum on day 5 in group B.The expression of IL-6 and IL-8 was gradually weakened with time in group C.The expression of IL-6 and IL-8 were high,and reached a maximum on day 5 and declined thereafter in group D.AD of positive cells in group D were higher than group B at each time point(P<0.05).The time which 0.49 N orthodontic force was loaded was longer,orthodontic tooth movement distance was greater.Movement distance in group D were longer than group B(P<0.05).Conclusions:Orthodontic force as well as inflammatory stimulus can evoke the expression of IL-6 and IL-8.Under the combined effects of inflammation and orthodontic force,the expression of IL-6,IL-8will increase.展开更多
A simple overview of daily orthodontic practice involves use of brackets, wires and elastomeric modules. However, investigating the underlying effect of orthodontic forces shows various molecular and cellular changes....A simple overview of daily orthodontic practice involves use of brackets, wires and elastomeric modules. However, investigating the underlying effect of orthodontic forces shows various molecular and cellular changes. Also, orthodontics is in close relation with dentofacial orthopedics which involves bone regeneration. In this review current and future applications of stem cells(SCs) in orthodontics and dentofacial orthopedics have been discussed. For craniofacial anomalies, SCs have been applied to regenerate hard tissue(such as treatment of alveolar cleft) and soft tissue(such as treatment of hemifacial macrosomia). Several attempts have been done to reconstruct impaired temporomandibular joint. Also, SCs with or without bone scaffolds and growth factors have been used to regenerate bone following distraction osteogenesis of mandibular bone or maxillary expansion. Current evidence shows that SCs also have potential to be used to regenerate infrabony alveolar defects and move the teeth into regenerated areas. Future application of SCs in orthodontics could involve accelerating tooth movement, regenerating resorbed roots and expanding tooth movement limitations. However, evidence supporting these roles is weak and further studies are required to evaluate the possibility of these ideas.展开更多
背景:正畸力通过多种信号通路激活牙周组织自噬,进一步增强或减弱相关细胞(牙周膜细胞、骨细胞、破骨细胞和成骨细胞等)的活性来促进牙周重塑。目的:综述目前正畸力介导牙周组织自噬的研究进展和其对正畸牙齿移动的影响。方法:在PubMed...背景:正畸力通过多种信号通路激活牙周组织自噬,进一步增强或减弱相关细胞(牙周膜细胞、骨细胞、破骨细胞和成骨细胞等)的活性来促进牙周重塑。目的:综述目前正畸力介导牙周组织自噬的研究进展和其对正畸牙齿移动的影响。方法:在PubMed、Web of Science、中国生物医学文献数据库和中国知网数据库进行文献检索,设置检索时限为2010-2023年,总结了2010年以来正畸与自噬相关研究进展,最终纳入76篇文献进行分析讨论。结果与结论:(1)正畸力可通过牙周机械感受器和其造成的无菌性炎症引发一系列生物化学信号的转变,进而引起牙周组织自噬。(2)自噬通过级联放大的信号通路如磷脂酰肌醇-3-激酶/蛋白激酶B通路、河马通路及丝裂原活化蛋白激酶通路等,产生相应反馈从而促进牙周组织改建,最终实现牙齿的移动与稳定。正畸力诱导的自噬可差异性调节牙齿压力侧骨吸收和张力侧骨形成,相关靶点在正畸临床治疗的应用中具有良好前景。(3)然而,正畸力与自噬的机制较为复杂,现有研究仅停留在探究自噬对正畸牙齿移动的作用,自噬与正畸牙齿移动过程中的相互调节作用、涉及相关通路上游机械受体及信号通路间的交互作用均需要进一步的探究。展开更多
基金supported by Department of Science and Technology in Henan Province(No 122300410407)
文摘Objective:To investigate effect of orthodontic force on inflammatory periodontal tissue remodeling and expression of IL-6 and IL-8 in rats.Methods:Eighty SD rats were randomly divided into 4 groups,blank control group(group A)with 5 rats,treatment normal group(group B)with 25 rats,inflammation control group(group(group C)with 25 rats,inflammation treatment group(group D)with 25 rats.Immunohistochemistry and histomorphometric analysis was performed to measure the expression of IL-6,IL-8 and the firet molar to the recent movement in the distance.Results:The expression of IL-8 reached a maximum on day 5 and declined thereafter in group B;the expression of IL-6 reached a maximum on day 5 in group B.The expression of IL-6 and IL-8 was gradually weakened with time in group C.The expression of IL-6 and IL-8 were high,and reached a maximum on day 5 and declined thereafter in group D.AD of positive cells in group D were higher than group B at each time point(P<0.05).The time which 0.49 N orthodontic force was loaded was longer,orthodontic tooth movement distance was greater.Movement distance in group D were longer than group B(P<0.05).Conclusions:Orthodontic force as well as inflammatory stimulus can evoke the expression of IL-6 and IL-8.Under the combined effects of inflammation and orthodontic force,the expression of IL-6,IL-8will increase.
文摘A simple overview of daily orthodontic practice involves use of brackets, wires and elastomeric modules. However, investigating the underlying effect of orthodontic forces shows various molecular and cellular changes. Also, orthodontics is in close relation with dentofacial orthopedics which involves bone regeneration. In this review current and future applications of stem cells(SCs) in orthodontics and dentofacial orthopedics have been discussed. For craniofacial anomalies, SCs have been applied to regenerate hard tissue(such as treatment of alveolar cleft) and soft tissue(such as treatment of hemifacial macrosomia). Several attempts have been done to reconstruct impaired temporomandibular joint. Also, SCs with or without bone scaffolds and growth factors have been used to regenerate bone following distraction osteogenesis of mandibular bone or maxillary expansion. Current evidence shows that SCs also have potential to be used to regenerate infrabony alveolar defects and move the teeth into regenerated areas. Future application of SCs in orthodontics could involve accelerating tooth movement, regenerating resorbed roots and expanding tooth movement limitations. However, evidence supporting these roles is weak and further studies are required to evaluate the possibility of these ideas.
文摘目的：观察兔实验性正畸牙齿移动过程中VEGFR-1在牙周组织中的表达。方法：选用体重在2.0 kg左右的日本大耳白兔35只,分为正常组与实验1、3、5、7、14、21天组。建立兔正畸牙齿移动模型,对实验标本进行VEGFR-1免疫组化染色。通过组织学观察,计算机图像分析系统对兔牙周组织中VEGFR-1的表达变化进行平均灰度分析,进行统计学处理与分析。结果：在正常牙周组织中,VEGFR-1有所表达,局限分布于新生血管内皮细胞。牙周组织受到正畸压力后,压力区1-7 d VEGFR-1的表达与正常牙周组织相比,差异显著（P〈0.01）;在张力区在3-14 d时,VEGFR-1阳性反应灰度积分与正常牙周组织相比,差异显著（P〈0.01）。高峰值压力区出现在牙齿受力后第5天,张力区出现在第5天。结论：正常兔牙周组织中存在VEGFR-1;VEGFR-1参与了兔实验性正畸牙齿移动牙周组织的改建过程。
文摘背景:正畸力通过多种信号通路激活牙周组织自噬,进一步增强或减弱相关细胞(牙周膜细胞、骨细胞、破骨细胞和成骨细胞等)的活性来促进牙周重塑。目的:综述目前正畸力介导牙周组织自噬的研究进展和其对正畸牙齿移动的影响。方法:在PubMed、Web of Science、中国生物医学文献数据库和中国知网数据库进行文献检索,设置检索时限为2010-2023年,总结了2010年以来正畸与自噬相关研究进展,最终纳入76篇文献进行分析讨论。结果与结论:(1)正畸力可通过牙周机械感受器和其造成的无菌性炎症引发一系列生物化学信号的转变,进而引起牙周组织自噬。(2)自噬通过级联放大的信号通路如磷脂酰肌醇-3-激酶/蛋白激酶B通路、河马通路及丝裂原活化蛋白激酶通路等,产生相应反馈从而促进牙周组织改建,最终实现牙齿的移动与稳定。正畸力诱导的自噬可差异性调节牙齿压力侧骨吸收和张力侧骨形成,相关靶点在正畸临床治疗的应用中具有良好前景。(3)然而,正畸力与自噬的机制较为复杂,现有研究仅停留在探究自噬对正畸牙齿移动的作用,自噬与正畸牙齿移动过程中的相互调节作用、涉及相关通路上游机械受体及信号通路间的交互作用均需要进一步的探究。
