Objective To establish a rapid and effective supercritical fluid extraction（SFE） and rapid resolution liquid chromatography method coupled with diode-array detector（RRLC-DAD） to quantify the chromones in a species...Objective To establish a rapid and effective supercritical fluid extraction（SFE） and rapid resolution liquid chromatography method coupled with diode-array detector（RRLC-DAD） to quantify the chromones in a species. Methods The effects of four parameters including ethanol concentration（50%-90%）, pressure（25-45 MPa）, temperature（40-60 ℃）, and time（30-90 min） on the chromones yields, namely prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, and sec-O-glucosylhamaudol, were investigated using SFE system with orthogonal array design（OAD）. Furthermore, the extracts were analyzed using rapid resolution liquid chromatography coupled with diode-array detector（RRLC-DAD） system to confirm the results. Results Under the optimized conditions, i.e., 35 MPa of pressure, 60 ℃ of temperature, 70% ethanol, and 60 min of time, the yields of prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, sec-O-glucosylhamaudol, and total chromones were 3.514, 0.132, 6.242, 0.342, and 10.231 mg/g, respectively. In comparison with ultrasonic assisted extraction（UAE）, SFE was able to yield a 20.7% increase in the total chromones from Saposhnikoviae Radix. Conclusion SFE is an alternative and promising method to extract chromones from this species, and the established RRLC-DAD method could serve as a rapid and effective method for the identification of chromones from Saposhnikoviae Radix.展开更多
基金National Natural Science Foundation of China(Grant No.31300270)the Technology Key Project of Jilin Province(Grant No.20130522048JH)the twelfth five-year science and technology plan project of Jilin Education Department(2015205)
文摘Objective To establish a rapid and effective supercritical fluid extraction（SFE） and rapid resolution liquid chromatography method coupled with diode-array detector（RRLC-DAD） to quantify the chromones in a species. Methods The effects of four parameters including ethanol concentration（50%-90%）, pressure（25-45 MPa）, temperature（40-60 ℃）, and time（30-90 min） on the chromones yields, namely prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, and sec-O-glucosylhamaudol, were investigated using SFE system with orthogonal array design（OAD）. Furthermore, the extracts were analyzed using rapid resolution liquid chromatography coupled with diode-array detector（RRLC-DAD） system to confirm the results. Results Under the optimized conditions, i.e., 35 MPa of pressure, 60 ℃ of temperature, 70% ethanol, and 60 min of time, the yields of prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, sec-O-glucosylhamaudol, and total chromones were 3.514, 0.132, 6.242, 0.342, and 10.231 mg/g, respectively. In comparison with ultrasonic assisted extraction（UAE）, SFE was able to yield a 20.7% increase in the total chromones from Saposhnikoviae Radix. Conclusion SFE is an alternative and promising method to extract chromones from this species, and the established RRLC-DAD method could serve as a rapid and effective method for the identification of chromones from Saposhnikoviae Radix.
