It is suggested that the isolates of Oryctes Nudivirus (OrNV), cultured for decades in cells of Heteronychus arator (F.) (HA), be checked to verify genomic changes have not occurred which adapt them to culture but red...It is suggested that the isolates of Oryctes Nudivirus (OrNV), cultured for decades in cells of Heteronychus arator (F.) (HA), be checked to verify genomic changes have not occurred which adapt them to culture but reduce or cancel their ability to infect the target pest, the coconut rhinoceros beetle (CRB), Oryctes rhinoceros (L.). Full genomes of field-caught OrNV isolates, and their infectivity against larvae and adults, could be compared with those of HA-cultured isolates. Further data to correlate OrNV dosage indices with doses in number of virions/ml could be advantageous so as to explore if CRB larvae or adults may resist infection by a sub-threshold dose. Also the possibility of changes in the HA culture cells which alter the outer coat of the resulting virion, hence perhaps its infectivity towards CRB cells, could be checked. Might it be possible to move beyond HA-culture and develop tissue culture of Oryctes rhinoceros cells for mass production of OrNV as this beetle species is the target? Nuclear genomes of OrNV-resistant and OrNV-susceptible strains of the CRB could be examined for changes perhaps correlated with resistance. The possibility of endosymbiotic bacteria affecting CRB susceptibility to OrNV might be checked.展开更多
Laboratory and field studies were conducted on some aspects of biology and control methods of fruit stalk borer Oryctes elegans Prell., the study was conducted in date palm orchards in south Baghdad during the years 2...Laboratory and field studies were conducted on some aspects of biology and control methods of fruit stalk borer Oryctes elegans Prell., the study was conducted in date palm orchards in south Baghdad during the years 2009 and 2010. The laboratory rearing result ofO. elegans indicated that the larval stage period was very long reached 6 months, the body weight of the mature larva was 7.61 gm, the length and body circumference were 7.58 and 5.23 cm respectively. The period of pupa stage was 19 day and 2.97 gm body weight, while the length and body circumference were 3.74 and 3.21 cm respectively .While the weight of adult stage was 1.67 gm and 3.14 cm, 2.71 cm for the length and circumference body respectively. Results of hand collection larvae in palm trees during the regular annual service, showed reduction in population density of adults to 1.4 adult/trap per week compared with 3.3 and 14.0 adult/trap per week in the orchards were larvae collected partially and without hand collection by using the light traps with solar energy （Magna Traps, Russell IPM） respectively. Also, the study proved the existence of an inverse relation between the moon light and adult activity, which affected on the adult number cough in light traps. These results indicated that the hand collection of larvae works during months January, February, March and April at synchronized with the larval stage on palm trees, and use Magna Traps as an effective method to control O. elegans through IPM programs in palm orchards.展开更多
The European rhinoceros beetle, Oryctes nasicornis (L.), is a large flying beetle in the subfamily Dynastinae of family Scarabaeidae and order Coleoptera. It is well-known to be a Palaearctic species, inhabiting Europ...The European rhinoceros beetle, Oryctes nasicornis (L.), is a large flying beetle in the subfamily Dynastinae of family Scarabaeidae and order Coleoptera. It is well-known to be a Palaearctic species, inhabiting Europe and Asia up to North Africa, with no reported material from the Sub-Saharan African region including Tanzania. The present work reports an occurrence of O. nasicornis in Tanzania specifically in Iringa region in the Southern Highlands zone of the country. The report is accompanied by description of the key morphological characters of the insect validating its diagnosis. The findings facilitate a basis for intervention of the existing records about O. nasicornis, including its biogeographical affiliation and the related undertakings. It is most likely that the species also ranges in other localities in Tanzania and the Sub-Saharan Africa region in general because of the existing proximity and interactions.展开更多
文摘It is suggested that the isolates of Oryctes Nudivirus (OrNV), cultured for decades in cells of Heteronychus arator (F.) (HA), be checked to verify genomic changes have not occurred which adapt them to culture but reduce or cancel their ability to infect the target pest, the coconut rhinoceros beetle (CRB), Oryctes rhinoceros (L.). Full genomes of field-caught OrNV isolates, and their infectivity against larvae and adults, could be compared with those of HA-cultured isolates. Further data to correlate OrNV dosage indices with doses in number of virions/ml could be advantageous so as to explore if CRB larvae or adults may resist infection by a sub-threshold dose. Also the possibility of changes in the HA culture cells which alter the outer coat of the resulting virion, hence perhaps its infectivity towards CRB cells, could be checked. Might it be possible to move beyond HA-culture and develop tissue culture of Oryctes rhinoceros cells for mass production of OrNV as this beetle species is the target? Nuclear genomes of OrNV-resistant and OrNV-susceptible strains of the CRB could be examined for changes perhaps correlated with resistance. The possibility of endosymbiotic bacteria affecting CRB susceptibility to OrNV might be checked.
文摘Laboratory and field studies were conducted on some aspects of biology and control methods of fruit stalk borer Oryctes elegans Prell., the study was conducted in date palm orchards in south Baghdad during the years 2009 and 2010. The laboratory rearing result ofO. elegans indicated that the larval stage period was very long reached 6 months, the body weight of the mature larva was 7.61 gm, the length and body circumference were 7.58 and 5.23 cm respectively. The period of pupa stage was 19 day and 2.97 gm body weight, while the length and body circumference were 3.74 and 3.21 cm respectively .While the weight of adult stage was 1.67 gm and 3.14 cm, 2.71 cm for the length and circumference body respectively. Results of hand collection larvae in palm trees during the regular annual service, showed reduction in population density of adults to 1.4 adult/trap per week compared with 3.3 and 14.0 adult/trap per week in the orchards were larvae collected partially and without hand collection by using the light traps with solar energy （Magna Traps, Russell IPM） respectively. Also, the study proved the existence of an inverse relation between the moon light and adult activity, which affected on the adult number cough in light traps. These results indicated that the hand collection of larvae works during months January, February, March and April at synchronized with the larval stage on palm trees, and use Magna Traps as an effective method to control O. elegans through IPM programs in palm orchards.
文摘The European rhinoceros beetle, Oryctes nasicornis (L.), is a large flying beetle in the subfamily Dynastinae of family Scarabaeidae and order Coleoptera. It is well-known to be a Palaearctic species, inhabiting Europe and Asia up to North Africa, with no reported material from the Sub-Saharan African region including Tanzania. The present work reports an occurrence of O. nasicornis in Tanzania specifically in Iringa region in the Southern Highlands zone of the country. The report is accompanied by description of the key morphological characters of the insect validating its diagnosis. The findings facilitate a basis for intervention of the existing records about O. nasicornis, including its biogeographical affiliation and the related undertakings. It is most likely that the species also ranges in other localities in Tanzania and the Sub-Saharan Africa region in general because of the existing proximity and interactions.
