Oryza sativa L. ssp. japonica and indica exhibit different sensitivity to photoinhibition and they show different stability of their core proteins D1 in the chloroplast photosystem Ⅱ. Using in situ hybridization, psb...Oryza sativa L. ssp. japonica and indica exhibit different sensitivity to photoinhibition and they show different stability of their core proteins D1 in the chloroplast photosystem Ⅱ. Using in situ hybridization, psbA, the gene encoding D1 protein of O. sativa ssp. japonica cv. 9516, and that of O. sativa ssp. indica cv. Shanyou 63 was cloned. As revealed by homology comparison of their sequences, the sequences are identical in the regions of promoter and 5′-UTR; differences are found in individual bases in the coding region all of which, being in the third position of respective codons, however, do not affect the amino acids coded finally; a difference is noted in the length of the oligo-U sequence in the region of 3′-UTR. It is thus apparent that, rather than a result of any difference in the amino acid sequences, the differences in the sensitivity to photoinhibition of D1 proteins between japonica and indica rice may be related to the upstream factors that regulate expression of psbA or to differences of photoprotective mechanisms.展开更多
We discovered 528 putative cytochrome P450s (P450s) in Oryza sativa L. ssp. indica using Arabidopsis thaliana P450s as database. Those putative rice P450s are thought to belong to 40 families classified in Arabidopsis...We discovered 528 putative cytochrome P450s (P450s) in Oryza sativa L. ssp. indica using Arabidopsis thaliana P450s as database. Those putative rice P450s are thought to belong to 40 families classified in Arabidopsis thaliana. We compared distributions of Arabidopsis thaliana and Oryza sativa P450s and found the two species have similar distribution patterns. However, family distributions of two species also have some differences. For example, in rice, the gene number in families of CYP71, CYP72, CYP76, CYP89, CYP94 and CYP709 is more than twice that in Arabidopsis thaliana; and there are 33 CYP705 members in Arabidopsis thaliana but none in rice. We also found gene members in CYP71 and CYP81 are organized as tandem arrays repeated in the rice genome; maybe they are duplications in the evolutionary event. Furthermore, we accumulated expression sequence tag (EST) evidence for 263 putative rice P450s, which are expressed at transcriptional level and more likely to be true P450s.展开更多
文摘Oryza sativa L. ssp. japonica and indica exhibit different sensitivity to photoinhibition and they show different stability of their core proteins D1 in the chloroplast photosystem Ⅱ. Using in situ hybridization, psbA, the gene encoding D1 protein of O. sativa ssp. japonica cv. 9516, and that of O. sativa ssp. indica cv. Shanyou 63 was cloned. As revealed by homology comparison of their sequences, the sequences are identical in the regions of promoter and 5′-UTR; differences are found in individual bases in the coding region all of which, being in the third position of respective codons, however, do not affect the amino acids coded finally; a difference is noted in the length of the oligo-U sequence in the region of 3′-UTR. It is thus apparent that, rather than a result of any difference in the amino acid sequences, the differences in the sensitivity to photoinhibition of D1 proteins between japonica and indica rice may be related to the upstream factors that regulate expression of psbA or to differences of photoprotective mechanisms.
文摘We discovered 528 putative cytochrome P450s (P450s) in Oryza sativa L. ssp. indica using Arabidopsis thaliana P450s as database. Those putative rice P450s are thought to belong to 40 families classified in Arabidopsis thaliana. We compared distributions of Arabidopsis thaliana and Oryza sativa P450s and found the two species have similar distribution patterns. However, family distributions of two species also have some differences. For example, in rice, the gene number in families of CYP71, CYP72, CYP76, CYP89, CYP94 and CYP709 is more than twice that in Arabidopsis thaliana; and there are 33 CYP705 members in Arabidopsis thaliana but none in rice. We also found gene members in CYP71 and CYP81 are organized as tandem arrays repeated in the rice genome; maybe they are duplications in the evolutionary event. Furthermore, we accumulated expression sequence tag (EST) evidence for 263 putative rice P450s, which are expressed at transcriptional level and more likely to be true P450s.
