Objective： To study possible immunobiological potential of Osmunda japonica Thunb. Methods： Immunomodulatory effects of ethanol extracts prepared from rhizomes of O. japonica and phenolic compounds isolated from the...Objective： To study possible immunobiological potential of Osmunda japonica Thunb. Methods： Immunomodulatory effects of ethanol extracts prepared from rhizomes of O. japonica and phenolic compounds isolated from the extracts were investigated under the in vitro conditions using the rat peritoneal cells （2 × 10_6/mL; 24 h culture）. Biosynthesis of nitric oxide （NO） was assayed by Griess reagent, production of prostaglandin E2 （PGE2） and secretion of cytokines were determined by enzyme-linked immunoabsorbent assay. Resalts： The extracts activated dose dependently, with the onset at 2.5-5 μ mol/L concentrations, the high output NO production, and secretion of interferon-r, （IFN-r）, tumor necrosis factor-oL （TNF-α） and interleukin-1 （IL-1 13 ）. Mild enhancement of NO was produced by the aldehyde-type phenolics 4-hydroxybenzaldehyde and 3,4-hydroxybenzaldehyde. In contrasts, the acetone-type phenolics 4-hydroxybenzalacetone and 3,4-hydroxybenzalacetone inhibited production of immune mediators including cytokines （TNF-α, IL-β, IL-6）, NO, and PGE2. The 3,4-hydroxybenzalacetone was more effective than 4-hydroxybenzaldehyde. The ICsoS estimates ranged within the interval of 5-10 μ mol/L. No signs of cytotoxicity were observed up to the 50 μ-mol/L concentration of the compounds. Coaclasiea： Phenolic compounds contained in medicinal herb Osmunda japonica possess distinct immunomodulatory activity.展开更多
基金Supported by the International Science and Technology Cooperation Program of the People's Republic of China(No.2011DFA30870)the bilateral grant from the Ministry of Education,Youth and Sports of the Czech Republic(No.ME10116)
文摘Objective： To study possible immunobiological potential of Osmunda japonica Thunb. Methods： Immunomodulatory effects of ethanol extracts prepared from rhizomes of O. japonica and phenolic compounds isolated from the extracts were investigated under the in vitro conditions using the rat peritoneal cells （2 × 10_6/mL; 24 h culture）. Biosynthesis of nitric oxide （NO） was assayed by Griess reagent, production of prostaglandin E2 （PGE2） and secretion of cytokines were determined by enzyme-linked immunoabsorbent assay. Resalts： The extracts activated dose dependently, with the onset at 2.5-5 μ mol/L concentrations, the high output NO production, and secretion of interferon-r, （IFN-r）, tumor necrosis factor-oL （TNF-α） and interleukin-1 （IL-1 13 ）. Mild enhancement of NO was produced by the aldehyde-type phenolics 4-hydroxybenzaldehyde and 3,4-hydroxybenzaldehyde. In contrasts, the acetone-type phenolics 4-hydroxybenzalacetone and 3,4-hydroxybenzalacetone inhibited production of immune mediators including cytokines （TNF-α, IL-β, IL-6）, NO, and PGE2. The 3,4-hydroxybenzalacetone was more effective than 4-hydroxybenzaldehyde. The ICsoS estimates ranged within the interval of 5-10 μ mol/L. No signs of cytotoxicity were observed up to the 50 μ-mol/L concentration of the compounds. Coaclasiea： Phenolic compounds contained in medicinal herb Osmunda japonica possess distinct immunomodulatory activity.
