Objective： To observe the effect of Gengnianchun Recipe （更年春方, GNC） on bone mineral density （BMD）, bone biomechanical parameters and serum lipid level in the bilaterally ovariectomized （OVX） rats and to exp...Objective： To observe the effect of Gengnianchun Recipe （更年春方, GNC） on bone mineral density （BMD）, bone biomechanical parameters and serum lipid level in the bilaterally ovariectomized （OVX） rats and to explore the prophylactic and therapeutic action of GNC on ovariectomy induced osteoporosis and hyperlipidemia. Methods： OVX SD rats, 10- 12 months old, were divided into different groups and fed with GNC 2 g/d, GNC 1 g/d and Nilestriol 0. 125 mg/week, respectively for 4 months to observe the change of BMD and bone biomechanical parameters of the lumbar vertebrae, and the serum levels of total cholesterol （TO）, triglyceride（TG）, high-density lipoprotein cholesterol （HDL-C） and low-density lipoprotein cholesterol （LDL-C）, and to compare the effect of the two drugs on the morphology of the uterus. Results： There was marked reduction in BMD and biomechanical parameters in lumbar vertebrae （ P〈0. 01 ） and increase of serum TO and LDL-C levels （ P〈0. 01 ） in rats after OVX. GNC or Nilestriol significantly improved the decreased BMD and biomechanical parameters of the lumbar vertebrae （ P〈0.05 or P〈0. 01 ）, and reduced the serum TO and LDL-C levels （ P〈0. 01 ）. In the Nilestriol group, the wet weight of uterus got increased obviously （ P〈0.01 ）, the number of uterine glands increased, uterine columnar epithelium thickened, and the mitotic figures in the epithelial stroma and myointimal cells augmented. But no such effect in wet weight and morphology of uterus was found in the GNC group. Conclusion： GNC could increase the BMD and biomechanical parameters of the lumbar vertebrae, reduce the serum TO and LDL-C levels, yet produce no adverse reaction in stimulating proliferation and hypertrophy of uterus.展开更多
目的：研究铸骨胶囊对去势大鼠骨质疏松症的治疗作用.方法：选用雌性6月龄SD大鼠60只,随机分为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ组（分别为假手术组,模型组,模型＋骨疏康颗粒组,模型＋铸骨胶囊高、中、低剂量组）,Ⅰ组在双侧卵巢位置切除与卵巢...目的：研究铸骨胶囊对去势大鼠骨质疏松症的治疗作用.方法：选用雌性6月龄SD大鼠60只,随机分为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ组（分别为假手术组,模型组,模型＋骨疏康颗粒组,模型＋铸骨胶囊高、中、低剂量组）,Ⅰ组在双侧卵巢位置切除与卵巢同等大小的脂肪块,其他各组（Ⅱ～Ⅵ）切除双侧卵巢造成绝经后骨质疏松症动物模型.Ⅰ、Ⅱ组按1 ml/100 g体重灌胃（1.33%的β-环糊精水溶液）,Ⅲ组按2.7g/kg体重灌胃（骨疏康颗粒水溶液）,Ⅳ、Ⅴ、Ⅵ组分别按400、200、100 mg/kg体重灌胃（铸骨胶囊水溶液）.给药3个月后,检测各组动物的左侧股骨及第2腰椎骨密度（bone mineral density,BMD）,股骨生物力学强度（用三点弯曲实验检测）,对胫骨切片进行骨组织形态计量学分析,采用放免法测血清中雌二醇（estradiol,E2）、降钙素（calcitonin,CT）、骨钙素（bone gal protein,BGP）含量.结果：切除雌性大鼠双侧卵巢3个月后,腰椎及股骨BMD明显降低,与Ⅰ组比差异有显著性统计意义（P＜0.05）,提示绝经后骨质疏松症动物模型复制成功.铸骨胶囊各组（Ⅳ、Ⅴ、Ⅵ）与Ⅱ组比较,Ⅳ组腰椎和股骨BMD增加,差异有显著性统计意义（P＜0.05,P＜0.01）.Ⅳ组股骨生物力学强度各项指标及胫骨骨组织形态计量参数明显改善,差异有显著性统计意义（P＜0.05）;CT、BGP含量升高,差异有显著性统计意义（P＜0.001,P＜0.01）.Ⅳ组与Ⅲ组比较,最大桡度增加,差异有显著性统计意义（P＜0.05）;骨小梁形成表面（trabeculaformation surface,TFS）百分比增加,差异有显著性统计意义（P＜0.01）;CT、BGP含量升高,差异有显著性统计意义（P＜0.05,P＜0.01）.结论：铸骨胶囊可以增加切除双侧卵巢大鼠的BMD,提高骨的力学性能,降低骨折的发生率,对去势大鼠有一定的抑制骨丢失作用.展开更多
文摘Objective： To observe the effect of Gengnianchun Recipe （更年春方, GNC） on bone mineral density （BMD）, bone biomechanical parameters and serum lipid level in the bilaterally ovariectomized （OVX） rats and to explore the prophylactic and therapeutic action of GNC on ovariectomy induced osteoporosis and hyperlipidemia. Methods： OVX SD rats, 10- 12 months old, were divided into different groups and fed with GNC 2 g/d, GNC 1 g/d and Nilestriol 0. 125 mg/week, respectively for 4 months to observe the change of BMD and bone biomechanical parameters of the lumbar vertebrae, and the serum levels of total cholesterol （TO）, triglyceride（TG）, high-density lipoprotein cholesterol （HDL-C） and low-density lipoprotein cholesterol （LDL-C）, and to compare the effect of the two drugs on the morphology of the uterus. Results： There was marked reduction in BMD and biomechanical parameters in lumbar vertebrae （ P〈0. 01 ） and increase of serum TO and LDL-C levels （ P〈0. 01 ） in rats after OVX. GNC or Nilestriol significantly improved the decreased BMD and biomechanical parameters of the lumbar vertebrae （ P〈0.05 or P〈0. 01 ）, and reduced the serum TO and LDL-C levels （ P〈0. 01 ）. In the Nilestriol group, the wet weight of uterus got increased obviously （ P〈0.01 ）, the number of uterine glands increased, uterine columnar epithelium thickened, and the mitotic figures in the epithelial stroma and myointimal cells augmented. But no such effect in wet weight and morphology of uterus was found in the GNC group. Conclusion： GNC could increase the BMD and biomechanical parameters of the lumbar vertebrae, reduce the serum TO and LDL-C levels, yet produce no adverse reaction in stimulating proliferation and hypertrophy of uterus.
文摘目的：研究铸骨胶囊对去势大鼠骨质疏松症的治疗作用.方法：选用雌性6月龄SD大鼠60只,随机分为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ组（分别为假手术组,模型组,模型＋骨疏康颗粒组,模型＋铸骨胶囊高、中、低剂量组）,Ⅰ组在双侧卵巢位置切除与卵巢同等大小的脂肪块,其他各组（Ⅱ～Ⅵ）切除双侧卵巢造成绝经后骨质疏松症动物模型.Ⅰ、Ⅱ组按1 ml/100 g体重灌胃（1.33%的β-环糊精水溶液）,Ⅲ组按2.7g/kg体重灌胃（骨疏康颗粒水溶液）,Ⅳ、Ⅴ、Ⅵ组分别按400、200、100 mg/kg体重灌胃（铸骨胶囊水溶液）.给药3个月后,检测各组动物的左侧股骨及第2腰椎骨密度（bone mineral density,BMD）,股骨生物力学强度（用三点弯曲实验检测）,对胫骨切片进行骨组织形态计量学分析,采用放免法测血清中雌二醇（estradiol,E2）、降钙素（calcitonin,CT）、骨钙素（bone gal protein,BGP）含量.结果：切除雌性大鼠双侧卵巢3个月后,腰椎及股骨BMD明显降低,与Ⅰ组比差异有显著性统计意义（P＜0.05）,提示绝经后骨质疏松症动物模型复制成功.铸骨胶囊各组（Ⅳ、Ⅴ、Ⅵ）与Ⅱ组比较,Ⅳ组腰椎和股骨BMD增加,差异有显著性统计意义（P＜0.05,P＜0.01）.Ⅳ组股骨生物力学强度各项指标及胫骨骨组织形态计量参数明显改善,差异有显著性统计意义（P＜0.05）;CT、BGP含量升高,差异有显著性统计意义（P＜0.001,P＜0.01）.Ⅳ组与Ⅲ组比较,最大桡度增加,差异有显著性统计意义（P＜0.05）;骨小梁形成表面（trabeculaformation surface,TFS）百分比增加,差异有显著性统计意义（P＜0.01）;CT、BGP含量升高,差异有显著性统计意义（P＜0.05,P＜0.01）.结论：铸骨胶囊可以增加切除双侧卵巢大鼠的BMD,提高骨的力学性能,降低骨折的发生率,对去势大鼠有一定的抑制骨丢失作用.
