Mitochondrial genome has been widely used in species identification and gene conservation.In the present study,the complete mitochondrial genome of Oula sheep(Ovis aries)was determined using next-generation sequencing...Mitochondrial genome has been widely used in species identification and gene conservation.In the present study,the complete mitochondrial genome of Oula sheep(Ovis aries)was determined using next-generation sequencing.This genome was16 618 bp(NCBI accession number:KU575248)and contained 13 protein coding genes,22 transfer RNA genes,two ribosomal RNA genes,and a typical control region.The overall nucleotide composition was 33.7%A,27.4%T,25.8%C,and 13.1%G,with a total A+T content of 61.1%.The phylogenetic analysis of selected sheep breeds showed that Oula sheep were clustered within branch A and originated from approximately 6 ka.This mitochondrial genome will provide valuable information for molecular genetic research of Oula sheep.展开更多
探讨Double sex和mab-3相关转录因子7(Double sex and mab-3related transcription factor7,Dmrt7)在不同发育期绵羊睾丸组织中的表达规律和定位情况,本试验选取0、2、6、12和24月龄健康绵羊15只,每组3只,采集睾丸组织,运用实时荧光定量...探讨Double sex和mab-3相关转录因子7(Double sex and mab-3related transcription factor7,Dmrt7)在不同发育期绵羊睾丸组织中的表达规律和定位情况,本试验选取0、2、6、12和24月龄健康绵羊15只,每组3只,采集睾丸组织,运用实时荧光定量PCR、Western blot和免疫组织化学染色方法检测不同月龄睾丸组织中Dmrt7的表达与定位情况。结果显示,在不同月龄绵羊睾丸组织中均检测到Dmrt7mRNA和蛋白,并且表达趋势较为一致,但不同月龄睾丸中表达量存在差异;在0、2和6月龄绵羊睾丸中,Dmrt7mRNA和蛋白表达量很低,而在12和24月龄表达量较高,并且极显著高于0、2和6月龄(P<0.01);0、2和6月龄绵羊睾丸组织中免疫阳性反应定位于少量的精原细胞,而在12和24月龄睾丸组织中免疫阳性反应主要定位于次级精母细胞和部分初级精母细胞及精子细胞。研究结果表明,Dmrt7可能对各级生精细胞的分裂过程尤其是初级精母细胞通过两次减数分裂形成精子细胞的过程起着重要的调控作用。展开更多
基金Supported by the Central Public-Interest Scientific Institution Basal Research Fund(1610322016006)Science and Technology Support Projects in Gansu Province(1504NKCA052)the Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2014-LIHPS-01)
文摘Mitochondrial genome has been widely used in species identification and gene conservation.In the present study,the complete mitochondrial genome of Oula sheep(Ovis aries)was determined using next-generation sequencing.This genome was16 618 bp(NCBI accession number:KU575248)and contained 13 protein coding genes,22 transfer RNA genes,two ribosomal RNA genes,and a typical control region.The overall nucleotide composition was 33.7%A,27.4%T,25.8%C,and 13.1%G,with a total A+T content of 61.1%.The phylogenetic analysis of selected sheep breeds showed that Oula sheep were clustered within branch A and originated from approximately 6 ka.This mitochondrial genome will provide valuable information for molecular genetic research of Oula sheep.
文摘探讨Double sex和mab-3相关转录因子7(Double sex and mab-3related transcription factor7,Dmrt7)在不同发育期绵羊睾丸组织中的表达规律和定位情况,本试验选取0、2、6、12和24月龄健康绵羊15只,每组3只,采集睾丸组织,运用实时荧光定量PCR、Western blot和免疫组织化学染色方法检测不同月龄睾丸组织中Dmrt7的表达与定位情况。结果显示,在不同月龄绵羊睾丸组织中均检测到Dmrt7mRNA和蛋白,并且表达趋势较为一致,但不同月龄睾丸中表达量存在差异;在0、2和6月龄绵羊睾丸中,Dmrt7mRNA和蛋白表达量很低,而在12和24月龄表达量较高,并且极显著高于0、2和6月龄(P<0.01);0、2和6月龄绵羊睾丸组织中免疫阳性反应定位于少量的精原细胞,而在12和24月龄睾丸组织中免疫阳性反应主要定位于次级精母细胞和部分初级精母细胞及精子细胞。研究结果表明,Dmrt7可能对各级生精细胞的分裂过程尤其是初级精母细胞通过两次减数分裂形成精子细胞的过程起着重要的调控作用。
