The effect of Radix Salviae Miltiorrhizae (RSM) on the gene expression of nitric oxide synthase (NOS) in rat brains during ischemia was studied with in situ hybridization and the results were analyzed with IBAS 2000 I...The effect of Radix Salviae Miltiorrhizae (RSM) on the gene expression of nitric oxide synthase (NOS) in rat brains during ischemia was studied with in situ hybridization and the results were analyzed with IBAS 2000 Image Analysis System. It was found that NOS gene expression of cerebral cortex and caudate-putamen was markedly increased in 24 hours in ischemia group (P展开更多
AIM:In order to explore whether the member of Bcl-2 gene family,for example,B cl-2 and Bax,are induced after cerebral ischemia,and whether expression of gene s can be modulated by calcium-antagonist.METHODS:The rat ce...AIM:In order to explore whether the member of Bcl-2 gene family,for example,B cl-2 and Bax,are induced after cerebral ischemia,and whether expression of gene s can be modulated by calcium-antagonist.METHODS:The rat cerebral ischemic mode ls were made by Nagasawa and Zea Longa improvement method,by occluding left midd le cerebral artery;the expression of Bcl-2 and Bax mRNA were measured by RT-PC R method.RESULTS:After administration of nimodipine, Bcl-2 mRNA was up-regulat ed in the hippocampus at 6 and 24 h after ischemia,while Bax mRNA was down-regu lated at 6 and 24h after ischemia.CONCLUSION:Calcium-antagonist can up-regulat e Bcl-2 mRNA and down-regulate Bax mRNA.Increasing the ratio of Bcl-2 and Bax mRNA may contribute to the anti-apoptic effect of nimodipine.展开更多
Objective:To explore the protective mechanism of acupuncture plus mild hypothermia for cerebral ischemia-reperfusion injury(CIRI)by observing the effects of acupuncture plus mild hypothermia on miRNA-204 and its targe...Objective:To explore the protective mechanism of acupuncture plus mild hypothermia for cerebral ischemia-reperfusion injury(CIRI)by observing the effects of acupuncture plus mild hypothermia on miRNA-204 and its target gene expressions in CIRI rat brain tissues.Methods:Sixty Sprague-Dawley rats were divided into a blank control group,a sham operation group,a model group,an acupuncture group,a mild hypothermia group and an acup un cture plus mild hypothermia group accordi ng to the random nu mbertable method(n=10).Except for the blank c on trol group an dthe sham operati on group,rats in the other 4 groups received CIRI modeling.After the model was successfully established,rats in the blank control group were bred routinely for 72 h without any interventions;rats in the sham operation group and the model group were bred routinely for 72 h,and only received bin ding without other interve ntions after surgery;rats in the acup un cture group were bred routinely for 72 h,and received acupuncture at Dazhui(GV 14),Baihui(GV 20)and Shuigou(GV 26)after binding;rats in the mild hypothermia group were bred routinely for 72 h,and received mild hypothermia intervention for 72 h after binding;rats in the acupuncture plus mild hypothermia group were bred routinely for 72 h,followed by receiving acupuncture as in the acup uncture group and mild hypothermia therapy as in the mild hypothermia group after bin ding.The n eurological impairme nt score,cerebral infarcti on area ratio,the expressions of miRNA-204 and its target genes in eluding Map3k8,Ntrk2 and Ppp3rl in the ischemic hippocampus of each group were observed after 72 h of intervention.Results:Before intervention,compared with the bgnk control group and the sham operation group,the neurological impairment scores and the infarction area ratios of the modelled rats were statistically significa ntly increased(all P<Q.Ql)f indicating that the model was successful.After intervention,compared with the model group,the neurological impairment scores of the three in tervention groups were sign ifica ntly reduced(all P<0.01);compared with the acupuncture group and the mild hypothermia group,the infarction area ratio in the acupuncture plus mild hypothermia group was signtiicantly reduced(both P<0.01);compared with the model group,the three intervention groups showed significant inhibition of miRNA-204 expression in brain tissues(all P<0.05),which was most significant in the acupuncture plus mild hypothermia group(P<0.01);compared with the acupuncture group and the mild hypothermia group,the Map3k8 expression in the acupuncture plus mild hypothermia group was significantly increased(both P<0.01),but there were no sign ificant d iff ere nces in Ntrk2 and Ppp3rl expressions between groups(all P>0.05).Conclusion:Acupuncture,mild hypothermia,and acupuncture plus mild hypothermia reduced the neurological impairment score and the cerebral infarction area in CIRI rats,while acupuncture plus mild hypothermia showed the most significant effect.In regulating miRNA-204 target gene expressions,acupuncture plus mild hypothermia showed the same effect on Ntrk2 and Ppp3rl expressions,while better effect on Map3k8 expression compared with either acupuncture or hypothermia.展开更多
OBJECTIVE:To observe the regulation of electroacupuncture on gene expression at calcium signaling pathways in mice with cerebral ischemia reperfusion.METHODS:Sixty male, inbred Kunming mice were randomly assigned to t...OBJECTIVE:To observe the regulation of electroacupuncture on gene expression at calcium signaling pathways in mice with cerebral ischemia reperfusion.METHODS:Sixty male, inbred Kunming mice were randomly assigned to three groups:repeated cerebral ischemia reperfusion group(RG, n = 24),sham-operated group(SG, n = 12), and electroacupuncture group(EG, n = 24).Mice in RG and EGgroups were modeled by repeated cerebral ischemia reperfusion surgery, and EG mice were treated with electroacupuncture for 30 min after recovery from anesthesia.Changes in gene expression profile of mice hippocampi were analyzed by global expression profile microarray.Genes that were up-regulated or down-regulated greater than 1.5folds were considered to be biologically meaningful.Real-time quantitative polymerase chain reaction(q-PCR) method was used to verify the expression of selected genes based on the algorithm [2^(ΔΔCt)].RESULTS:Compared with SG mice, 242 genes showed different in expressions in RG mice:107down-regulated and 135 up-regulated.Compared with RG mice, 609 genes showed a difference of expression in EG mice:315 down-regulated and 375up-regulated.Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses indicated two pathways:calcium signaling and long-term potentiation in which 11 differentially expressed genes selected.Six of the 11 genes in the calcium signaling pathway were verified after real-time q-PCR testing.CONCLUSION:Electroacupuncture treatment of cerebral ischemia reperfusion appears to regulate Atp2a2, Cacna1 e, Camk2 a, Gnas, Grm1, Rapgef3 genes in the calcium signaling pathway.展开更多
文摘The effect of Radix Salviae Miltiorrhizae (RSM) on the gene expression of nitric oxide synthase (NOS) in rat brains during ischemia was studied with in situ hybridization and the results were analyzed with IBAS 2000 Image Analysis System. It was found that NOS gene expression of cerebral cortex and caudate-putamen was markedly increased in 24 hours in ischemia group (P
文摘AIM:In order to explore whether the member of Bcl-2 gene family,for example,B cl-2 and Bax,are induced after cerebral ischemia,and whether expression of gene s can be modulated by calcium-antagonist.METHODS:The rat cerebral ischemic mode ls were made by Nagasawa and Zea Longa improvement method,by occluding left midd le cerebral artery;the expression of Bcl-2 and Bax mRNA were measured by RT-PC R method.RESULTS:After administration of nimodipine, Bcl-2 mRNA was up-regulat ed in the hippocampus at 6 and 24 h after ischemia,while Bax mRNA was down-regu lated at 6 and 24h after ischemia.CONCLUSION:Calcium-antagonist can up-regulat e Bcl-2 mRNA and down-regulate Bax mRNA.Increasing the ratio of Bcl-2 and Bax mRNA may contribute to the anti-apoptic effect of nimodipine.
文摘Objective:To explore the protective mechanism of acupuncture plus mild hypothermia for cerebral ischemia-reperfusion injury(CIRI)by observing the effects of acupuncture plus mild hypothermia on miRNA-204 and its target gene expressions in CIRI rat brain tissues.Methods:Sixty Sprague-Dawley rats were divided into a blank control group,a sham operation group,a model group,an acupuncture group,a mild hypothermia group and an acup un cture plus mild hypothermia group accordi ng to the random nu mbertable method(n=10).Except for the blank c on trol group an dthe sham operati on group,rats in the other 4 groups received CIRI modeling.After the model was successfully established,rats in the blank control group were bred routinely for 72 h without any interventions;rats in the sham operation group and the model group were bred routinely for 72 h,and only received bin ding without other interve ntions after surgery;rats in the acup un cture group were bred routinely for 72 h,and received acupuncture at Dazhui(GV 14),Baihui(GV 20)and Shuigou(GV 26)after binding;rats in the mild hypothermia group were bred routinely for 72 h,and received mild hypothermia intervention for 72 h after binding;rats in the acupuncture plus mild hypothermia group were bred routinely for 72 h,followed by receiving acupuncture as in the acup uncture group and mild hypothermia therapy as in the mild hypothermia group after bin ding.The n eurological impairme nt score,cerebral infarcti on area ratio,the expressions of miRNA-204 and its target genes in eluding Map3k8,Ntrk2 and Ppp3rl in the ischemic hippocampus of each group were observed after 72 h of intervention.Results:Before intervention,compared with the bgnk control group and the sham operation group,the neurological impairment scores and the infarction area ratios of the modelled rats were statistically significa ntly increased(all P<Q.Ql)f indicating that the model was successful.After intervention,compared with the model group,the neurological impairment scores of the three in tervention groups were sign ifica ntly reduced(all P<0.01);compared with the acupuncture group and the mild hypothermia group,the infarction area ratio in the acupuncture plus mild hypothermia group was signtiicantly reduced(both P<0.01);compared with the model group,the three intervention groups showed significant inhibition of miRNA-204 expression in brain tissues(all P<0.05),which was most significant in the acupuncture plus mild hypothermia group(P<0.01);compared with the acupuncture group and the mild hypothermia group,the Map3k8 expression in the acupuncture plus mild hypothermia group was significantly increased(both P<0.01),but there were no sign ificant d iff ere nces in Ntrk2 and Ppp3rl expressions between groups(all P>0.05).Conclusion:Acupuncture,mild hypothermia,and acupuncture plus mild hypothermia reduced the neurological impairment score and the cerebral infarction area in CIRI rats,while acupuncture plus mild hypothermia showed the most significant effect.In regulating miRNA-204 target gene expressions,acupuncture plus mild hypothermia showed the same effect on Ntrk2 and Ppp3rl expressions,while better effect on Map3k8 expression compared with either acupuncture or hypothermia.
基金Supported by the 2013 National Natural Science Foundation of China:The Effect of Electro-acupuncture for Mediating Jnk Mitochondrial Pathway in Bax-gene Knockout Mice With the Cerebral Ischemia Reperfusion(No.81373731)
文摘OBJECTIVE:To observe the regulation of electroacupuncture on gene expression at calcium signaling pathways in mice with cerebral ischemia reperfusion.METHODS:Sixty male, inbred Kunming mice were randomly assigned to three groups:repeated cerebral ischemia reperfusion group(RG, n = 24),sham-operated group(SG, n = 12), and electroacupuncture group(EG, n = 24).Mice in RG and EGgroups were modeled by repeated cerebral ischemia reperfusion surgery, and EG mice were treated with electroacupuncture for 30 min after recovery from anesthesia.Changes in gene expression profile of mice hippocampi were analyzed by global expression profile microarray.Genes that were up-regulated or down-regulated greater than 1.5folds were considered to be biologically meaningful.Real-time quantitative polymerase chain reaction(q-PCR) method was used to verify the expression of selected genes based on the algorithm [2^(ΔΔCt)].RESULTS:Compared with SG mice, 242 genes showed different in expressions in RG mice:107down-regulated and 135 up-regulated.Compared with RG mice, 609 genes showed a difference of expression in EG mice:315 down-regulated and 375up-regulated.Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses indicated two pathways:calcium signaling and long-term potentiation in which 11 differentially expressed genes selected.Six of the 11 genes in the calcium signaling pathway were verified after real-time q-PCR testing.CONCLUSION:Electroacupuncture treatment of cerebral ischemia reperfusion appears to regulate Atp2a2, Cacna1 e, Camk2 a, Gnas, Grm1, Rapgef3 genes in the calcium signaling pathway.
